The Cartridge ® Using the Trueprep AUTO v2 ® Charging/Using the Trueprep AUTO v2 with the AC Adapter Plugging in the reagent bottles ® Switching ON/OFF the Trueprep AUTO v2 Placing the Cartridge in the device and starting the run...
Figure 1: TheTrueprep AUTO v2 Top View, with cartridge holder shown in the front panel ® Figure 2: The Trueprep AUTO v2 device Back Panel with Reagent Pack of the ® Trueprep AUTO v2 Universal Cartridge Based Sample Prep Kit ®...
The PCR process necessitates the extraction and purification of nucleic acids from clinical ® specimens to free it from potential PCR inhibitors. The Trueprep AUTO v2 Universal ® Cartridge Based Sample Prep Device together with Trueprep AUTO v2 Universal Cartridge Based Sample Prep Kit provides an easy method of nucleic acid extraction and purification.
Do not install the Trueprep AUTO v2 next to instruments that may cause vibrations or electromagnetic interference. ® Do not store the Trueprep AUTO v2 in the path of direct sunlight or use it close to any radiating or heating apparatus, such as a conventional oven, hot plate or infrared lamp. ®...
EJECT button Cartridge Holder Door ® Figure 1 : The Trueprep AUTO v2 Top View, with cartridge holder shown in the front panel ® The Power button switches on and switches off the Trueprep AUTO v2 The Eject button opens the cartridge holder The Start button is used to begin sample processing ®...
The Power/In Use LED Indicator will glow when theTrueprep AUTO v2 is switched on and when it is in use. ® It will glow red and blink continuously when the Trueprep AUTO v2 is switched on and waiting for user input ii) It will glow red continuously when it is in use ®...
The Charging/Low Battery LED Indicator will glow red and you will also hear a beeping ® sound. The Trueprep AUTO v2 device can not begin a new run if the battery is low. Fig 4: Low battery LED glowing red 7.1.3 If the battery charge is full or sufficient to carry out a run, the Charging/Low Battery LED Indicator...
Trueprep AUTO ® Figure 5: The Charging Port in right hand side of the Back Panel of the Trueprep AUTO v2 7.1.4 Connect the other end of the AC Adapter to a mains socket. 7.1.5 Switch On the mains power.
Page 10
7.2.2 Plugging in the reagent pack of 50T pack size: ® 1. Before starting a run for the first time, plug in the reagent pack from the Trueprep AUTO v2 Sample Prep Kit to the backside of the device (Figure 8). Discard the used reagent pack in a proper manner.
Page 11
® Trueprep AUTO 7.2.5 Buffer Resetting Procedure When buffer count is completed (buffer count=25/50), the device will prompt for changing Reagent pack and resetting buffer count. 7.2.5.1 When QR code scanning disabled: Following will be displayed on screen alternatively, once buffer count reaches 25/50. SHUTTING DOWN CHANGE REAGENT BY PRESSING START...
® 7.3 Switching ON/OFF the Trueprep AUTO v2 ® 7.3.1 Press the Power button to switch on the Trueprep AUTO v2. You will be prompted with the message ‘Buffer Count’. BUFFER COUNT 7.3.2 Later you will be prompted with the message for TRUEPREP AUTO 7.3.3 Press the Power button, when following screen will be displayed, release the power button.
Page 13
AUTO ® 7.4.2 Take a cartridge out of the Cartridge Pouch from the Trueprep AUTO v2 Universal Cartridge Based Sample Prep Kit Cartridge pack. The pouch also contains one Elute Collection Tube (ECT) for use at the end of extraction. Place the cartridge, after adding pre-treated sample (sample + lysis reagent) into the sample chamber (see procedure in corresponding Truenat™...
Page 14
Clean the cartridge holder with 70% Isopropyl Alcohol (IPA) as described in Section 8.3. iv. Replace the removed tray with a new tray and close the cartridge holder (25 Replaceable ® trays are provided with the Trueprep AUTO v2 device. More trays, if required, can be ordered from Molbio). Replaceable Tray...
The Screen displays the error message E1:CARTRIDGE “E1:CARTRIDGE VALVE ERROR” and VALVE ERROR instruction “Press Eject to exit” alternatively. Solution: Press Eject Contact Molbio’s support team to exit E2:CARTRIDGE The Screen displays the error message ERROR “E2:CARTRIDGE ERROR” and instruction “Press Eject to exit” alternatively.
Page 16
The Screen displays the error message E6:CARTRIDGE “E6:CARTRIDGE NOT LOADED” and NOT LOADED instruction “Press Eject to exit” alternatively. Press Eject Solution: Contact Molbio’s support team to exit Reagent Pack The Screen displays the error message E7:EXPIRED Errors and “E7:EXPIRED RESET CARD” and...
Page 17
The Screen displays the alert message Device W1:SBC Errors and “W1:SBC NOT RESPONDING” for 2 NOT Responding warnings seconds. Solution: Contact Molbio’s support team The Screen displays the error message “E11:RTD-L ERROR” E11:RTD- E11:RTD- Note: This is for Lysis Heater L ERROR L ERROR Solution: Contact Molbio’s support team...
8.2 User Menu 8.2.1 Accessing Menu ® Press and hold ‘Start Button’ and ‘Power Button’ on the Trueprep AUTO v2. Firmware Version Ÿ and Total run status counts will be displayed on the screen as given below. Firmware Ver: X.X...
Page 19
® Trueprep AUTO - EXIT- - EXIT- < < > > Note: Shutdown is disabled in menu; in order to shutdown the device, user must exit from menu and press Power Button. 8.2.3 Flushing Procedure Replace Reagent Pack for 25T or 50T with Distilled water, using provided Plug-in connector. Ÿ...
70% Isopropyl Alcohol (IPA). Wait for 5 minutes before inserting a new Replaceable tray into the holder. 8.3.4 Disposal of Instrumentation Do not Dispose off Instrument as unsorted municipal waste. Contact Molbio for collection of instrument. ®...
Page 21
® Trueprep AUTO In Vitro Diagnostic Medical Device ®...
Page 22
® Trueprep AUTO This Side Up THIS SIDE UP Medical Electronic Rush MEDICAL ELECTRONIC RUSH Do Not Stack DO NOT STACK Fragile FRAGILE Keep Dry KEEP DRY Fully automatic operation Portable Rapid sample prepping Mains / rechargeable battery powered ® True prep AUTO v2 Universal Cartridge...
Need help?
Do you have a question about the Trueprep AUTO v2 and is the answer not in the manual?
Questions and answers