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CID CI-340 Instruction Manual

Handheld photosynthesis system
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Summary of Contents for CID CI-340

  • Page 1 4 9 0 1...

  • Page 2: Table Of Contents

    Care of the CI-340............................. 8-1 Leaf Chamber – Care and Use........................9-1 Recharging............................... 10-1 To Power your CI-340 From a Lab Power Socket ................10-1 To Disconnect the Charger/Adapter ....................10-2 To Charge the Li-ion Battery ......................10-2 Battery Charger Specifications ......................10-3 Equations ..............................
  • Page 3 Setup Procedures..........................D-1 Appendix E Canopy Chamber – Assembly Instructions ................E-1 Appendix F CI-510CF Chlorophyll Fluorescence Module ............... F-1 Connecting the CI-510CF Chlorophyll Fluorescence Module to the CI-340 Main Unit....F-1 CI-340 Fluorescence Saturation Pulse Measurement ................. F-1 Specifications............................F-3...

  • Page 4: Introduction

    CI-340 TECHNICAL SUPPORT If you have a question about the CI-340 features and functions, first look in the CI-340 Instruction Manual. If you cannot find the answer, you can contact a Technical Support Representative located in your country.
  • Page 5 Customers sometimes find that they need CID, Inc. to make a change to upgrade their system, or repair their system. In order for CID, Inc. to do these services, the customer must first contact us and obtain a Return Merchandise Authorization (RMA) number. Please contact a customer service representative for specific instructions when returning a product.

  • Page 6: Technical Information

    TECHNICAL INFORMATION TECHNICAL INFORMATION The CI-340 is a highly technologically advanced photosynthesis system. It contains a pump along with a mass airflow sensor. A built-in microprocessor regulates the airflow rate, which is set by the user. A technical diagram (Figure 2-1) illustrates a flow chart for this instrument. An illustration of the CI-340 is on the following page (Figure 2-2).
  • Page 7 TECHNICAL INFORMATION   Figure 2-2. Parts of the CI-340 3/31/2008 Rev. 4.3...

  • Page 8: General Operation Instructions

    GENERAL OPERATING INSTRUCTIONS GENERAL OPERATING INSTRUCTIONS This section will familiarize you with start-up procedures and guide you on how to move from one function to another. Specific instructions for each separate function will be found under their individual heading in the main body of the manual. For greatest accuracy, the instrument should be turned on 30 minutes prior to any calibrations to allow it to be fully warmed up.

  • Page 9: Using The Keypad

    GENERAL OPERATING INSTRUCTIONS USING THE KEYPAD The keypad for the CI-340 consists of 20 keys to enter commands and data. Figure 3-1 illustrates the keypad. Figure 3-1. CI-340 Keypad Pressing a key makes a “beeping” sound. A key should be pressed individually each time a command or letter is asked for.

  • Page 10: File Menu

      FILE MENU The CI-340 has a file system that allows a great range of data to be stored internally. It has been designed to emulate the familiar DOS of personal computers. Note that this menu will not be accessible when there are no files stored in memory; the message “no files” on the top display line will be briefly displayed when this situation occurs.

  • Page 11: Installation Of The Battery

    Deleting a file removes it permanently! Be sure to transfer important data before deleting it from the CI-340. Refer to the DATA TRANSFER section for related information.

  • Page 12: Interpretation Of Parameters

    INTERPRETATION OF PARAMETERS INTERPRETATION OF PARAMETERS (in) The amount of CO (in ppm) at the inlet of the analyzer (out) The amount of CO (in ppm) at the outlet of the leaf chamber (dif) The difference between the CO in and CO out values.
  • Page 13 INTERPRETATION OF PARAMETERS “Calibrate H O” Allows the user to calibrate the zero and span for H O measurements using external sources “Calibrate Temp” Allows the user to set the TSCAL1 and TSCAL2 parameters of the temperature sensor. “Calibrate Flow” Allows the user to calibrate the flow meter to external standards.

  • Page 14: System Setup / Calibration

    The system is shipped from the factory calibrated for immediate use. The user can perform most system calibrations if necessary. To start the CI-340, press . After a brief warm-up time, the instrument will display the basic display as follows:...

  • Page 15: Calendar And Time Setup

    ENTER to select Calibrate Temp No further calibration is allowed. ATMOSPHERIC PRESSURE The CI-340 pressure sensor is capable of measuring absolute atmospheric pressure. This value is used 3/31/2008 Rev. 4.3...

  • Page 16: Flow Rate

    FLOW RATE Figure 5-0 The CI-340 is capable of maintaining a steady airflow once the unit begins taking measurements. After the initial warm-up time (one minute), the instrument will generate the entered flow rate to regulate the accuracy of the measurements. The instrument is capable of automatically controlling the flow rate from 0.2 ~ 0.999 lpm.

  • Page 17: Co2 And H2O

    SYSTEM SETUP / CALIBRATION 5-0). A well-calibrated flow meter with little backpressure should be used as the standard. If for any reason an error was made during the calibration procedure pressing the key will also return back to the beginning of the flow calibration procedure without saving any changes made. Once the calibration procedure is competed, in a few moments the instrument will reset and return back to the above screen display.
  • Page 18 SYSTEM SETUP / CALIBRATION Figure 5-1. Configuration using compressed gas. After the system zero is established, use a gas with known concentration of CO to calibrate span. Use the key to skip the “calibrate the span” setting. Use known CO Press the key to proceed.
  • Page 19 SYSTEM SETUP / CALIBRATION NOTE O span setting should not be calibrated except once every six months. However, it is suggested that it be checked often by conducting a photosynthesis test with the “loop back” tube in place of a leaf chamber and sampling a known humidity of the atmosphere. Figure 5-2.

  • Page 20: Data Transfer

    DL.exe. Connect the CI-340 USB cable. Run the DL.exe. program to download code from the computer. Make certain that the the power is on to the CI-340. If the power does not stay on, then press and hold the key on the CI-340 (a constant ‘beep’...
  • Page 21 DATA TRANSFER you can stop pressing the key. The download of new code will take about 2 minutes. The computer screen will confirm that the procedure has been completed and the CI-340 will turn itself off. Press the key and the new software code is active.

  • Page 22: A Sample Data File

    DATA TRANSFER A SAMPLE DATA FILE Internal T Flow Pressure leaf 2out 27.5 100.09 2175 31.1 32.8 344.9 333.0 1.09 1.10 3.12 27.5 100.09 2011 31.2 32.9 339.9 328.2 1.23 1.25 2.78 27.5 100.09 2230 31.2 33.1 365.7 337.9 1.20 1.23 2.66 RHin...

  • Page 23: Photosynthesis, Transpiration And Stomatal Conductance

    It can be obtained by measuring the transpiration and leaf surface temperature (°C), and applying the calculation for Equation 4 found in the EQUATIONS chapter. The CI-340 is designed so that measurements can incorporate both absolute and differential readings simultaneously. This instrument can also be configured to operate both open and closed systems. It is important that the CI-340 uses the manufacturer’s current software.
  • Page 24 The instrument will then utilize all the same operating parameters as the last measurement. The CI-340 utilizes a shift key feature to accommodate many functions it has to offer. To register alpha-characters (such as A, H, R, etc.), use a sequence of the key followed by the respective key to obtain the letter.
  • Page 25 “3”. If both the CI-301CS and the CI-301LA are used, enter number “5”. If the CI-301AD and the CI- 301LA are used, enter number “6”. If all the three accessories are used, enter number “7”. To operate the CI-340 without any accessories, enter number “0”, or just press key. If one or more...

  • Page 26: Closed And Open Systems

    PHOTOSYNTHESIS, TRANSPIRATION AND STOMATAL CONDUCTANCE are using fully covers the leaf chamber window, enter the area of the window. Table 7-1 and 7-2 at the end of this chapter list the window sizes of all the leaf chambers CID, Inc. manufactures. Flow rate? (lpm) Enter the intended flow rate (in lpm).
  • Page 27 Determine the best size chamber (Table 7-1) for the measurements to be taken. Proceed to insert and attach the leaf chamber to the CI-340. Refer to the LEAF CHAMBER — CARE AND USE chapter for proper applications of the leaf chamber(s). Then press the key(s) to begin the measurement process.
  • Page 28 The closed system environment deals with chambers of variable volume. Each individual chamber is designed to interchange with the CI-340; however, they serve different sizes of the plant element. There are four available sizes of leaf chambers to conduct experiments for the closed system method (Table 7-2).

  • Page 29: Data Display Screens

    With the use of the CI-510LA (CI-301LA), the response curve of photosynthesis vs. light can be seen by pressing “L” when measuring photosynthesis (that is shift, shift, shift 4). The CI-340 will ask for the number of steps for light response and then will direct the CI-510LA to increase the light intensity from very low to very high in that number of steps.
  • Page 30 PHOTOSYNTHESIS, TRANSPIRATION AND STOMATAL CONDUCTANCE clockwise. Only the CI-510AD ordered with the CI-340 can be automatically controlled by the CI- 340 to generate the CO response curve. The response curve of photosynthesis vs. air temperature can be seen by pressing “T” (shift, shift, 7) when measuring photosynthesis.

  • Page 31: Care Of The Ci-340

    • Do not allow water or excessive moisture to get into the instrument. The CI-340 can measure air with high humidity, but it is highly recommended to avoid such circumstances. In addition, if used in the field environment, please avoid extremely dusty conditions.

  • Page 32: Leaf Chamber - Care And Use

    A chamber without O-rings on the tubes may demonstrate leakage at the CI-340 connection. To attach the chamber to the CI-340, carefully slide the tubes on the chamber into the “head” end of the instrument, aligning the locking screw (from the CI-340) with the mating hole in the chamber.
  • Page 33 We recommend working in temperature ranges between 5 and 45 ºC for greatest precision. Be sure both sensors are connected to the leaf chamber prior to using. The PAR sensor is extremely sensitive to even subtle changes in the light environment. Figure 9-2. Connecting a leaf chamber to the CI-340. 3/31/2008 Rev. 4.3...

  • Page 34: Recharging

    1. Connect the DC coupler to the CI-340. Align the front end of the DC coupler with the guides on the back of the CI-340, then press and slide the DC coupler into the CI-340 to set it in place.

  • Page 35: To Disconnect The Charger/Adapter

    RECHARGING TO DISCONNECT THE CHARGER/ADAPTER 1. Turn the CI-340 off and detach the DC coupler. 2. Disconnect the DC coupler from the charger/adapter. 3. Unplug the charger/adapter and disconnect the power cable from the charger/adapter. TO CHARGE THE Li-ion BATTERY Figure 10-2.

  • Page 36: Battery Charger Specifications

    DC to AC inverters, etc. • Use only specified CI-340 products with this charger/adapter. • Do not disassemble the charger/adapter (or DC coupler), and do not expose it to water, shock or vibration, or to direct sunlight.

  • Page 37: Equations

    EQUATIONS 3/31/2008 Rev. 4.3 11-1...
  • Page 38 EQUATIONS 3/31/2008 Rev. 4.3 11-2...
  • Page 39 EQUATIONS 3/31/2008 Rev. 4.3 11-3...

  • Page 40: Troubleshooting

    “blank” out the screen. Excessive glare may be the obvious problem • Make sure the accessory cable (if plugged into the CI-340) is in the “OFF” position, towards the instrument, or simply unplug the cable connector. The switch should only be in the “ON”...
  • Page 41 TROUBLESHOOTING • The sample(s) may not be a good source. • Ambient, internal operating temperatures may be too extreme. Check with the instrument accessory specifications for ideal operating conditions. Analyzer displays zero values for CO O concentrations . . . •...

  • Page 42: System Specifications

    O range: 0 to 100% RH Sensor: The CI-340 uses a humidity sensitive capacitor to measure the relative humidity. Its range is 0-100% with an accuracy of +/-2% at 10% RH and +/-3.5% at 90% RH. The response time of the sensor is about 15 seconds.
  • Page 43 SYSTEM SPECIFICATIONS PAR Sensor Type: Filtered GaAsP photodiode Range: 0 ~ 2500 µmol m Accuracy: 5 µmol m Response: 400 ~ 700 nm AIR Temperature Sensor Type: Thermocouple. Range: -15 ~ 50°C Accuracy: ± 0.1°C Leaf Temperature Sensor Type: Infrared. Range: -10 ~ 50°C Accuracy: ±...

  • Page 44: Power Pack / Accessory Bag

    POWER PACK / ACCESSORY BAG POWER PACK The CI-340 Battery Pack is necessary to operate the modules to control light, temperature, CO2 concentration, humidity level and chlorophyll fluorescence measurement. CI-340 Battery Pack (Figure 14-1) includes two rechargeable batteries, AC power supply charger (Figure 14-2), carrying bag, and cable connections for optional modules.

  • Page 45: Accessory Control Port And Cable

    POWER PACK / ACCESSORY BAG ACCESSORY CONTROL PORT AND CABLE Figure 14-4. CI-340 Accessory Control Port. The colored jacks on the accessory control cable (Figure 14Last printed 3/31/2008 9:13:00 AM-5) indicate the accessory / module to plug into. The red plug goes to the CI-510CS, the blue to the CI- 301LA, green to the CI-510CF, and the yellow plug to the CI-301AD.

  • Page 46: Ci-510Cs Temperature Control Module

    APPENDIX A APPENDIX A CI-510CS TEMPERATURE CONTROL MODULE The CI-510CS Temperature Control Module consists of two components (see Figure A-1 and A-2): A controller and Temperature Control Attachment. The CI-510CS allows you to increase or decrease the temperature ±25°C from ambient temperature. CAUTION: Avoid any obstruction or contact with the fan guards, especially small objects and fingers.

  • Page 47: Filling The Reservoir

    APPENDIX A FILLING RESERVOIR Attach one of the hoses from the Temperature Control Attachment to the “OUT” fitting (Figure A-4). There is no polarity; it does not matter which hose is used. A water connection kit is provided in a plastic bag.

  • Page 48: Emptying The Reservoir

    APPENDIX A Figure A-5 EMPTYING RESERVOIR Care for the CI-510CS Temperature Control System as you would any other sensitive instrument. The water in the hose needs to be completely drained after your experiment is finished. Empty the reservoir, by leaving the “IN” port on the controller panel open to air. Connect one hose from the Temperature Control Attachment to the “OUT”...

  • Page 49: Operating Temperature Control Module

    WARNING: Only the provided power source can be used for the CI-510CS. Using other power sources or power adapters may damage the CI-340 and the module. This will void the warranty. Figure A-8. Configuration for the CI-510CS Temperature Control Module.
  • Page 50 APPENDIX A To operate the CI-510CS, turn the power on. Temperature can be adjusted to a cooler or warmer state with the “Temperature” knob. You can vary the setting according to the readout of your CI-340 Photosynthesis System. There is a “Remote Input” socket on the Controller panel. This can be used by remote control for the purpose of automatically adjusting the system.
  • Page 51 PAR intensity emitted from the lamp or the external PAR sensor can be utilized. It is recommended that if the cable plugs into the CI-340, the external PAR sensor should not be plugged in.
  • Page 52 APPENDIX B REMINDER: The CI-301LA Light Attachment intake and exhaust vents must remain unobstructed during use to avoid overheating and possible damage to the light source. Do not place the lamp exhaust vents face down Figure B-2. Configuration for the CI-301LA Light Attachment. 3/31/2008 Rev.

  • Page 53: Appendix C Ci-301Ad Adjustable H 2 O And Co 2 Control Module

    APPENDIX C APPENDIX C CI-301AD ADJUSTABLE H2O AND CO2 CONTROL MODULE GENERAL DESCRIPTION The CI-301AD provides a gas source with a CO concentration adjustable from approximately 0 to 2000 ppm at flow rates up to 0.5 lpm. The unit also allows adjustment of the gas humidity level from approximately 5% relative humidity to 20-30% above ambient humidity levels up to 95%.
  • Page 54 Remove all moisture on the controller panel. Plug the power connector to the jack marked “12V DC” on the panel. Before operation, connect the “OUT” port on the CI-301AD to the “INTAKE” port on the CI-340 (see Figure C-1). Adjust both control knobs to their minimum (fully counterclockwise) positions.

  • Page 55: Operation

    To enable the CI-340 instrument to control the CO O levels remotely, the accessory cable must be connected to the CI-340 and the yellow color-coded plug must be inserted into the remote control jack of the CI-301AD. Turn the CO knob fully counterclockwise and the H O knob fully clockwise.

  • Page 56: Soda Lime

    APPENDIX C Soda Lime The soda lime should last for 8 to 24 hours of operation under most circumstances. Actual operating time will depend upon ambient CO levels. The soda lime supplied is an indicating variety: it will turn slightly blue as it becomes exhausted. The output of the CI-301AD may become unstable and show an increase in the CO level as the soda lime reaches exhaustion.

  • Page 57: Silica Gel

    APPENDIX C Use the same procedure described under Operating Instructions – “Adding Consumable” to refill the soda lime tube. Replace the filters (see Filters section below). Remember to gently tap the tube to help settle the material. Keep all containers of fresh soda lime tightly sealed to prevent absorption of ambient CO Silica Gel The silica gel should last from four to eight hours of operation under most circumstances.

  • Page 58: Appendix D Soil Respiration Chamber

    The CI-301SR Soil Respiration chamber allows easy measurements of soil respiration using the Closed System mode of the CI-340. Connect the CI-301SR (see Figure D-1) to the CI-340 in the Closed System configuration (refer to the CLOSED SYSTEM section if you need further assistance). Insert the PAR sensor firmly into its respective slot on top of the soil respiration chamber (refer to the LEAF CHAMBER - CARE AND USE section).
  • Page 59 APPENDIX D Figure D-1. Configuration for the CI-301SR Soil Respiration Chamber 3/31/2008 Rev. 4.3 D - 2...

  • Page 60: Appendix E Canopy Chamber - Assembly Instructions

    Now, attach the Canopy Chamber Attachment to the CI-340 by gently sliding the exposed tubes into the holes and tightening the locking screw of the CI-340. Place the IR Temperature sensor firmly into the opening located on Attachment ‘A’. Plug the IR Temperature sensor connector into its CI-340 port.
  • Page 61 APPENDIX E Figure E-1. Configuration for the Canopy Chamber Attachment 3/31/2008 Rev. 4.3 E - 2...

  • Page 62: Appendix F Ci-510Cf Chlorophyll Fluorescence Module

    CI-340 instrument and the CI-510CF module. Connect the eight-pin connector of the Accessory control cable to the Accessory control Port (see Figure 2-2) on the end of the CI-340. Insert the plug with green color band into the RS232 jack on the CI-510CF control unit.
  • Page 63 APPENDIX F Low Fluorescence value: Fo - dark, Fs - ambient High Fluorescence value: Fm dark, Fms - ambient Ratio of DHL:H: Fv/Fm - dark, Y - ambient The Ratio is the only value that is not in A/D counts. It should be interpreted as 0.xxx where xxx is the number displayed in the table.

  • Page 64: Specifications

    APPENDIX F SPECIFICATIONS: Power: 10 to 30 VDC, 75mA (175mA during saturation pulse) Fuse: 0.5A FB 3AG A/D converter: 2000 counts Chlorophyll fluorescence trace data Fo, Fm, Fv//Fm Measured parameters: (dark adapted) Fs, Fms, Y (ambient illumination) Modulated light intensity: 0.25 uE at 12 mm Saturation light intensity: 3,000 uE at 12 mm...
  • Page 65 Any defect must be called to the attention of CID, Inc., Camas, Washington, USA, in writing, within 90 days after receipt of the unit.

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