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Microgem PDQeX Product Manual

Nucleic acid extractor. prepgem,forensicgem,phytogem.
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Download this manual See also: Instructions for Use Manual
PDQeX Nucleic Acid Extractor
Product Manual
prepGEM
forensicGEM
phytoGEM

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Summary of Contents for Microgem PDQeX

  • Page 1 PDQeX Nucleic Acid Extractor Product Manual prepGEM forensicGEM phytoGEM...

  • Page 2: Table Of Contents

    Contents Introduction to the PDQeX Page 3 Process Overview Page 4 Device Set-up Page 5 Loading and Unloading Pages 6-8 Settings Page 9 Date and Time Page 10 UV Treatment Pages 10-11 Pre-defined Programs Page 12 Custom Programs Page 13...

  • Page 3: Introduction To The Pdqex

    PDQeX Nucleic Acid Extractor The simplicity and minimal sample manipulation requirement of the PDQeX make it an attractive option for obtaining DNA samples. Jo-Ann Stanton, PhD Department of Anatomy, University of Otago New Zealand MicroGEM's PDQeX Nucleic Acid Extractor brings together powerful enzymes with an innovative extractor cartridge and purification matrix.

  • Page 4: Process Overview

    This multi-stage process takes place inside the extractor cartridge. 1. The cells are lysed by a cocktail of enzymes. This cocktail varies for different substrates. For example, the lysis of leaf tissue requires a complex mixture of hydrolases and a specific program on the PDQeX to activate them.

  • Page 5: Device Set-Up

    On/Off Do not place the PDQeX close to a RF transmitter. A low power transmitter, such as a cordless or mobile phone, should be at least 2M from the PDQeX. A powerful transmitter, such as a commercial TV or radio broadcast antenna, should be at least 25M away.
  • Page 6 Remember where you have placed the collection tubes. You will need to place the cartridges in the same position in the cartridge block. Replace the rack into its slot in the PDQeX and firmly push into place. Loading and Unloading...

  • Page 7: Loading And Unloading

    - otherwise you will contaminate the device. WARNING: The lower end of the PDQeX cartridge is designed to fit inside the collector tube. If it is above the upper lip of the tube, there is a risk of cross contamination. This problem may occur when lower profile collection plates or tubes are used.
  • Page 8 Operating Instructions Loading and unloading the PDQeX After running the device, the DNA will be in the collection tubes. First remove the PDQeX extractor cartridges and dispose. Slide out the drawer. Remove and cap the collection tubes and store at -20 °...

  • Page 9: Settings

    Operating Instructions Running your program The PDQeX is controlled by a touch-screen. The following section provides an overview to help navigate through the controls. 1. Home Screen The home screen provides three options: 1. System settings 2. UV treatment (sterilization) 3.

  • Page 10: Date And Time

    3. UV Treatment The PDQeX is fitted with four UV strip lights to reduce DNA contamination. Three lights are situated below the collection tube drawer. The drawer should be removed before treatment to allow the light to access the lower side of the heating block.
  • Page 11 Operating Instructions Press 'UV Treatment' on the home screen. The procedure takes 30 minutes and can be interrupted at any time. Press Interrupt to stop the UV treatment. After interrupting the program, press Return to go back to the home screen. After the treatment is complete, press Return to go back to the home screen.
  • Page 12 Operating Instructions 4. Running Pre-defined Programs on the PDQeX On the home screen, press Extract. Select the program you want to run. The temperature regimen will be shown. Press Start. The PDQeX will switch to a display showing the progress of the program.
  • Page 13 Operating Instructions 5. Writing a Custom Program on the PDQeX From the home screen, press Extract. Instead of choosing one of the pre-defined programs, it is possible to create a custom program. This can be useful for optimizing the PDQeX for specfic sample types.

  • Page 14: Error Messages

    6. Error Messages A few common mistakes may be detected by the device. The PDQeX has a sensor at the back of the collection tube drawer. If the drawer is not in place or is crooked, the device will not run.
  • Page 15 UV light la lumière UV Inspection and Maintenance The PDQeX has been designed to require no operator maintenance. Disposal of Waste Used PDQeX cartridge tubes should be disposed as hazardous waste in accordance with laboratory protocols. Cleaning, Sterilization, Inspection, and Maintenance...
  • Page 16 MicroGEM are trademarks in use by the MicroGEM group. * Registered with the United States Patent and Trademark Office. For information regarding device compliance with various agency standards, contact info@microgembio.com. MicroGEM is committed to minimal packaging and a vibrant, sustainable world. info@microgembio.com +1-434-529-8212...