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Summary of Contents for Schambeck RI 2012
- Page 1 RI 2012 Schambeck SFD GmbH Drieschweg 13A · D – 53604 Bad Honnef, Germany Telephone: +49 (0) 2224 9239 0 · Telefax: +49 (0) 2224 9239 20 http://www.schambeck-sfd.com...
- Page 2 Version 1.01 Printed: 28.10.2013 File: RI2012 V1.0 english 10-2013..doc Last modified: Makowski...
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Page 3: Table Of Contents
Schambeck SFD GmbH Refractive Index Detector RI2012 ABLE OF ONTENTS TABLE OF CONTENTS 1 CONTACT ADDRESS 2 PRECAUTION AND WARNINGS 3 ELECTRICAL WARNINGS 4 GENERAL WARNINGS 5 SPECIFICATIONS 6 PHYSICAL SPECIFICATIONS 7 GUARANTEE CONDITIONS 8 PARTS INCLUDED IN THE DELIVERY... - Page 4 Schambeck SFD GmbH Refractive Index Detector RI2012 13 FLOW PATHS IN THE RI2012 13.1 M ASCO V EASURING ODE WITH ALVE 13.2 P ASCO V URGE ODE WITH ALVE 14 OPERATING THE RI2012 DETECTOR 14.1 O VERVIEW IRMWARE ERSION 14.2 T...
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Page 5: Contact Address
Schambeck SFD GmbH Refractive Index Detector RI2012 ONTACT DDRESS In case of problems with your RI2012 refractive index detector please contact your local distributor or Schambeck SFD GmbH. Schambeck SFD GmbH Drieschweg 13 A D – 53604 Bad Honnef Germany... -
Page 6: Precaution And Warnings
■ NOTE Please read this manual carefully before working with the RI2012 refractive index detector. In case of any question please do not hesitate to contact you local distributor or Schambeck SFD GmbH directly. LECTRICAL ARNINGS ... -
Page 7: General Warnings
Schambeck SFD GmbH Refractive Index Detector RI2012 ENERAL ARNINGS To prevent damages of the RI2012 all capillaries and cables have to be checked for damages and leakages. For the disposal of inflammable and/or toxic solvents a plan for waste management has to be created. - Page 8 Schambeck SFD GmbH Refractive Index Detector RI2012 Solvents should be degassed before use with a refractive index detector. After use of salt containing solvents (such as buffers) the detector should be purged with distilled water. Make sure that the 6 bar (0.6 MPa) pressure limit of the flow cell is not exceeded.
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Page 9: Specifications
Schambeck SFD GmbH Refractive Index Detector RI2012 PECIFICATIONS Type of detection: Refractive Index 1.00 – 1.75 RIU Effective range for refractive index: 1000 µRIU Effective range: Optical null balance: In the whole effective range by adjusting the mirror Signal null balance:... -
Page 10: Physical Specifications
Schambeck SFD GmbH Refractive Index Detector RI2012 HYSICAL SPECIFICATIONS Dimensions: 300 mm * 175 mm * 440 mm (W * H * D) Weight: 9,6 kg Voltages: Mains adaptor with voltage selector 100 – 120 V 220 – 240 V... -
Page 11: Guarantee Conditions
Schambeck SFD GmbH Refractive Index Detector RI2012 UARANTEE CONDITIONS The term of guarantee depends on you local law. Beside this Schambeck SFD GmbH affords guarantee for at least 12 months beginning after purchase of the RI2012 refractive index detector. All instruments are tested and certified by Schambeck SFD GmbH quality control. -
Page 12: Parts Included In The Delivery
Schambeck SFD GmbH Refractive Index Detector RI2012 ARTS INCLUDED IN THE DELIVERY Quantity Description Refractive Index Detector RI2012 Stainless steel capillary, 0.25 mm inner diameter, 1.59 mm outer diameter (for sample entry) Stainless steel capillary, 1.00 mm inner diameter, 1.59 mm outer diameter (for sample exit) PTFE tube, 1.59 mm inner diameter, 2.80 mm outer diameter... -
Page 13: Front Panel And Keyboard
Schambeck SFD GmbH Refractive Index Detector RI2012 RONT ANEL AND EYBOARD 9.1 T HE DISPLAY The RI2012 refractive index detector is equipped with a liquid crystal display to show the current detector signal as well as system parameters. This display contains four lines with 20 characters each. -
Page 14: The Auto Zero Button
Schambeck SFD GmbH Refractive Index Detector RI2012 UTTON By pressing the AutoZero button the detector signal is internally set to Zero. To return to the formel (unmodified) signal, press the AutoZero button for approx. three seconds. URGE UTTON To flush the reference chamber of the flow cell with mobile phase press the... -
Page 15: The Enter Button
Schambeck SFD GmbH Refractive Index Detector RI2012 NTER UTTON Press the ENTER button to open the selected sub menu and to accept changes you performed on a parameter. PORT This is the entrance for the mobile phase coming from the column. Connect the capillary coming from the column to this port using screw connectors. -
Page 16: The Back Side Of The Ri2012
Schambeck SFD GmbH Refractive Index Detector RI2012 10 T RI2012 IDE OF THE 10.1 O UTPUT ONNECTORS The RI2012 refractive index detector has two analog and one digital signal output. 10.1.1 T NTEGRATOR IGNAL UTPUT If you want to record your data using an analog integrator device, connect the integrator using a shielded signal cable to the integrator output (Int. -
Page 17: Input -Connectors
Schambeck SFD GmbH Refractive Index Detector RI2012 10.2 I NPUT ONNECTORS The RI2012 refractive index detector has three digital (TTL) inputs. These can be used to control the system externally. 10.2.1 T XTERNAL TART IGNAL Use the digital input connector (Dig.) to connect an external start signal (e. g. generated by an autosampler or a manual injector) to the instrument. -
Page 18: Mass Screw
Schambeck SFD GmbH Refractive Index Detector RI2012 10.5 M CREW Use the mass screw to connect the shielding of the analog signal cable to the detector’s chassis. This will result in a smoother detector signal. 10.6 O PTICAL DJUST Remove the cover from RI2012. Inside the RI2012 you find the optical system in an extra housing. -
Page 19: Installation Of The Ri2012
Schambeck SFD GmbH Refractive Index Detector RI2012 11 I RI2012 NSTALLATION OF THE 11.1 C ONNECTING THE DETECTOR TO THE CHROMATOGRAPHY SYSTEM Connect the INLET on the front of the detector to the capillary which comes from the column of you chromatography system. To attach the column to the detector use a 1/16” stainless steel capillary with 0.25 mm inner diameter. -
Page 20: Connection To An Analog Data System
Schambeck SFD GmbH Refractive Index Detector RI2012 11.2 C ONNECTION TO AN NALOG YSTEM Use a small screwdriver to connect the signal cable to one of the two pin connectors which are delivered with your instrument. Connect the signal cable either to re integrator output (Int. -
Page 21: Principle Of Detection
Schambeck SFD GmbH Refractive Index Detector RI2012 12 P RINCIPLE OF ETECTION The RI2012 refractive index detector acts as a differential refractometer which measures the deflection of a light beam as a result of different refractive indices of solutions in the reference and the sample chamber of the flow cell. - Page 22 Schambeck SFD GmbH Refractive Index Detector RI2012 13 F RI2012 ATHS IN THE 13.1 M ASCO V EASURING ODE WITH ALVE If the purge mode is disabled (red LED is off) the valve is switched that way that the mobile phase does not pass the reference chamber of the flow cell.
- Page 23 Schambeck SFD GmbH Refractive Index Detector RI2012 13.2 P ASCO V URGE ODE WITH ALVE The purge mode is used to flush the reference chamber or the flow cell with fresh mobile phase (solvent). If the purge mode is activated the red LED will light up. The mobile phase now passes the reference chamber of the flow cell.
- Page 24 Schambeck SFD GmbH Refractive Index Detector RI2012 14 O RI2012 D PERATING THE ETECTOR 14.1 O VERVIEW IRMWARE ERSION RI2012 V1.0 06-2013.EN / 28.10.2013 http://www.schambeck-sfd.com...
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Page 25: The Button
Schambeck SFD GmbH Refractive Index Detector RI2012 14.2 T NITIALIZATION TAGE The RI2012 refractive index detector does not consume any energy when it is switched off. The mains switch can be found on the back side of the instrument. After switching on the detector starts to initialize the internal micro controller and a testing procedure starts to check several system parameters. - Page 26 Schambeck SFD GmbH Refractive Index Detector RI2012 14.3 T ORMAL EASURING After performing the internal test procedure the detector will switch automatically to the normal measuring mode (NormMode). Now internally several tasks are running: Data acquisition Data processing ...
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Page 27: The Menu
Schambeck SFD GmbH Refractive Index Detector RI2012 The following parameters are shown: SetHeating,°C Temperature control SigSMOOTH Signal smoothing, internally RecRANGE Recorder output settings RecOFFSET, mV Signal offset for recorder output RecMARKER Marker settings ComRS232 Settings for serial RS232 port Another function of the status line is the input of new parameter settings by the user. -
Page 28: The Enter
Schambeck SFD GmbH Refractive Index Detector RI2012 14.4 T EMPERATURE ETTINGS Since the refractive index is highly depending on the temperature the temperature of the optical bench of the RI2012 refractive index detector can be controlled by the micro controller. To set the desired temperature press the... - Page 29 Schambeck SFD GmbH Refractive Index Detector RI2012 The current temperature is below 9 °C or above 64 °C. In this case the error message “!” will be shown in the display and a periodic acoustic signal occurs. The acoustic signal will stop when the temperature reaches the range between 9 °C and 64 °C.
- Page 30 Schambeck SFD GmbH Refractive Index Detector RI2012 14.5 S ETTING IGNAL MOOTHING To adjust the grade of smoothing of the detector signal press the menu button until the following status line is shown in the display. There are four different modes for signal smoothing: ...
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Page 31: T He I Ntegrator
Schambeck SFD GmbH Refractive Index Detector RI2012 – S 14.6 S ETTING ECORDER ANGE IGNAL MPLIFICATION To adjust the signal amplification press the MENU button until you can see the following status line in the display. If the mode RecRANGE is activated it allows to amplify or attenuate the detector signal at the recorder output (RecOut). - Page 32 Schambeck SFD GmbH Refractive Index Detector RI2012 14.7 S ETTING ECORDER FFSET AND ECORDER XTENT In this sub menu you can adjust the recorder offset or the recorder extent. If the recorder adjust is activated a certain (defined) voltage will be added to each data point recorded by the detector.
- Page 33 Schambeck SFD GmbH Refractive Index Detector RI2012 14.7.2 R ECORDER XTENT To adjust the recorder extent press the ArrowUp button (▲) until the following status line is displayed. Using the ArrowUp button (▲) you can switch to higher maximum voltages, the ArrowDown (▼) button leads to smaller maximum voltages for the recorder output.
- Page 34 Schambeck SFD GmbH Refractive Index Detector RI2012 14.8 M ARKERS To modify settings concerning marker signals press the MENU button until the following status line is displayed: The function UseMarker can be used to send a marker signal to the recorder output. Is the parameter UseMarker set to NONE no marker signal can be sent to the recorder.
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Page 35: The Digital
Schambeck SFD GmbH Refractive Index Detector RI2012 14.9 S (RS232) ETTINGS FOR ERIAL To modify settings concerning the digital communication using the serial RS232 port press MENU button until the status line in the display changes to the following. Using this function ComRS232 you may adjust the parameters for the serial data interface. -
Page 36: Optical
Schambeck SFD GmbH Refractive Index Detector RI2012 14.10 M TRUCTURE IN ORMAL EASURING N o r m M o d e In this mode the user can adjust several parameters fort he operation of the RI2012 refractiv index detector. The Norm Mode is used to: - Display temperature [°C], optical balance [%%], and the current signal [mV]. - Page 37 Schambeck SFD GmbH Refractive Index Detector RI2012 15 T ERVICE Since version 3.x the internal firmware of the RI2012 refractive index detector contains the service mode. This mode allows the user to check more detailed system parameters for example in case of a malfunction of the instrument to get more detailed information to call the technical support.
- Page 38 Schambeck SFD GmbH Refractive Index Detector RI2012 15.1 F IRMWARE NFORMATION If you need detailed information about your instrument such as the firmware version number or the instrument’s serial number press the MENU button in service mode until the menu SwHwInfo is shown in the status line.
- Page 39 Schambeck SFD GmbH Refractive Index Detector RI2012 15.2 V To check internal settings of the refractive index detector the view fine mode can be activated. To access this mode press the MENU button until the sub menu ViewFine is shown in the status line.
- Page 40 Schambeck SFD GmbH Refractive Index Detector RI2012 The displayed shortcuts represent the following voltages: Diff Difference voltage. This is the difference between the voltages resulting from measuring light intensity on the sample and the reference side of the light sensor.
- Page 41 Schambeck SFD GmbH Refractive Index Detector RI2012 – A – C 15.3 A DJUSTING IGITAL NALOG ONVERTERS button until the sub menu “AdjuDACs” is shown in the display. Press the MENU Press the ENTER button to access the mode to adjust a digital to analog converter. The...
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Page 42: The Optical
Schambeck SFD GmbH Refractive Index Detector RI2012 Now the signal at the integrator output and the recorder output is exactly 0 mV. Press the ENTER button again to switch to the next voltage. The current voltage which is applied to the signal output is shown in the display. - Page 43 Schambeck SFD GmbH Refractive Index Detector RI2012 … 15.4.1 OW TO CALIBRATE button until the statement “Calibr’n” is To activate the calibration mode press the MENU shown in the status line. By pressing the ENTER button the calibration mode is opened. The following information will be shown in the display.
- Page 44 Schambeck SFD GmbH Refractive Index Detector RI2012 At this point flush the reference chamber and the sample chamber with at least 5 mL distilled water. It is recommended to use a disposable syringe during the calibration to press the solutions into the INLET port. When the first step is finished, press the ENTER button.
- Page 45 Schambeck SFD GmbH Refractive Index Detector RI2012 If the signal is stable the calibration factor is calculated and saved. The success of the calibration process is confirmed by a displayed message. Press the ENTER button to finish the calibration process. The new calibration factor is shown in the upper right corner of the display.
- Page 46 Schambeck SFD GmbH Refractive Index Detector RI2012 15.5 ServMode Overview This mode allows the user to get detailed information about the current status of RI2012. If the calibration mode is not entered no changes can be applied on the instrument.
- Page 47 Schambeck SFD GmbH Refractive Index Detector RI2012 15.6 Serial (RS232) Communication Refractive Index detector RI2012 – Serial RS232 Interface – Firmware V 5.02 The RS232 port is used for the communication of detector and computer. The following option can be used to acquire data and control RI2012 refractive index detector.
- Page 48 Schambeck SFD GmbH Refractive Index Detector RI2012 15.7 USB Communication USB -support The RI2012 - Interface supports USB 2.0 High speed. To use the RI2012 with USB you have to install a Silicon Labs driver. System Requirements Silicon Labs 8-bit microcontroller software requires one of the following operating systems: ...
- Page 49 Schambeck SFD GmbH Refractive Index Detector RI2012 16 M RI2012 AINTENANCE OF THE 16.1 I DENTIFYING OFTWARE If you need detailed information about your instrument such as the firmware version number or the instrument’s serial number you can get this information in the service mode of the detector.
- Page 50 Schambeck SFD GmbH Refractive Index Detector RI2012 16.2 RROR ESSAGES In case of some malfunction the RI2012 refractive index detector may display an error message. The most error will appear directly after switching on the instrument. 16.2.1 M ALFUNCTION OF THE...
- Page 51 Schambeck SFD GmbH Refractive Index Detector RI2012 16.2.2 ALFUNCTION OF THE NALOG IGITAL ONVERTER This error messages only occur directly after switching on the RI2012 refractive index detector. If this message “noAD” shows up the analog digital converter did not connect to the micro controller.
- Page 52 Schambeck SFD GmbH Refractive Index Detector RI2012 “S ” “S ” 16.2.3 RROR ESSAGE IGNAL TOO LOW IGNAL TOO LOW The intensity alarm message can show up directly after initialization of the instrument or during operation. There are two different ways of displaying this message.
- Page 53 Schambeck SFD GmbH Refractive Index Detector RI2012 16.2.4 IGNAL UT OF ANGE The error message of the type “OVER_ADxx” shows up if the analog digital converter gets a signal which is too high or too low. To localize the problem the “xx” in the error message is filled with a two letter code...
- Page 54 Schambeck SFD GmbH Refractive Index Detector RI2012 16.2.5 ESET ONFIGURATION This type of error might only occur when the instrument is switched on. If the error message “RC” is shown in the display the controller was not able to load the internal configuration during the initialization.
- Page 55 Schambeck SFD GmbH Refractive Index Detector RI2012 16.3 A DJUSTING THE OPTICAL SYSTEM By adjusting the optical system of the RI2012 refractive index detector it is possible to influence the light intensity reaching the two photodiodes of the light sensor.
- Page 56 Schambeck SFD GmbH Refractive Index Detector RI2012 Put the hex-wrench into one of the opened hole at the back side of optical system. ■ NOTE There are two adjustment screws for the mirror. Keep in mind that you might have to turn the other screw.
- Page 57 Schambeck SFD GmbH Refractive Index Detector RI2012 16.4 XCHANGE AND DJUSTMENT OF THE It might be possible that you need to re-adjust the light source due to some changes in the used light bulb due to the transport or you need to replace a burned out lamp.
- Page 58 Schambeck SFD GmbH Refractive Index Detector RI2012 Loosen the holding screw of the light source and remove the lamp. Loosen the screw connector of the lamp’s power supply at the pre-amplifier board (labelled: LAMPE) Place a new lamp in the holder and tighten the holding screw RI2012 V1.0 06-2013.EN / 28.10.2013...
- Page 59 Schambeck SFD GmbH Refractive Index Detector RI2012 Connect the power wires of the lamp with the screw connector on the pre-amplifier board. Connect the mains cable to the detector and switch on the instrument ■ NOTE The following steps need to be performed on the running instrument. There is the danger of electrical shock when touching electrical components.
- Page 60 Schambeck SFD GmbH Refractive Index Detector RI2012 Make sure that only 2/3 of the slit are illuminated by the light source. This is illustrated in the following figure. ■ NOTE The projected picture at the light sensor should be a sharp one. The full height of the photo diodes has to be illuminated by the light beam.
- Page 61 Schambeck SFD GmbH Refractive Index Detector RI2012 16.5 C HECKING IFFERENCE OLTAGES This chapter describes the check of the sum and difference voltages. These voltages result from different light intensities reaching the sample side and the reference side of the light sensor.
- Page 62 Schambeck SFD GmbH Refractive Index Detector RI2012 To find out which value is displayed in which column press the ENTER button. The display will change to the following view: To go back to the former view press the ENTER button again.
- Page 63 Schambeck SFD GmbH Refractive Index Detector RI2012 The voltages are calculated according to the following formulas Summ Smpl + Rfrn Smpl – Rfrn Diff Use a multimeter to check the lamp voltage at the test point on the circuit board. In both cases the lamp voltage should be set 3.7 ...
- Page 64 Schambeck SFD GmbH Refractive Index Detector RI2012 16.6 C HECK AND EPLACEMENT OF THE ALVE If the valve which is used to switch the path of the mobile phase in the normal measuring mode and purge mode needs to be replaces follow the following steps: ...
- Page 65 If you are not sure it the problems are caused by a contaminated flow cell or caused by other problem contact the technical service of you local distributor or Schambeck SFD GmbH directly.
- Page 66 If it is not possible to remove the contaminant from your flow cell it might be necessary to replace it with a new flow cell. Please contact your local distributor or Schambeck SFD GmbH directly for assistance.
- Page 67 Schambeck SFD GmbH Refractive Index Detector RI2012 16.8 T RI2012 EATING IRCUIT OF THE In the following chapter the complete specification of the RI2012 heating circuit is put together. In the normal measuring mode (NormMode) the temperature is displayed with one digit (e.
- Page 68 Schambeck SFD GmbH Refractive Index Detector RI2012 17 V OLTAGE OINTS The following list contains different test points on the main circuit board of the RI2012 refractive index detector. All voltages listed below are measured in relation to Ground GND or AGND. Both are connected to the instrument’s housing through the holding screws on the circuit board.
- Page 69 RI2012, analytical and micro , 0,25mm ID 90-ZBU1 Inlet port RI2012, preparativ, 0,75mm ID 90-ZBU1 Outlet port RI2012, analytical, 0,75mm ID Spare parts you can order from your local distributor or Schambeck SFD GmbH directly. RI2012 V1.0 06-2013.EN / 28.10.2013 http://www.schambeck-sfd.com...
- Page 70 Schambeck SFD GmbH Refractive Index Detector RI2012 ECLARATION OF ONFORMITY Konformitätserklärung Declaration of Conformity Declaration de Conformité Schambeck SFD GmbH Nous Anschrift Rhöndorfer Str. 51 Address D-53604 Bad Honnef Adress Germany erklären in alleiniger Verantwortung, dass das Produkt: declare under our sole responsibility, that the product: declarons sous notre seule responsibilité, que le produit:...
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