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MicroCal iTC200 system Operating Instructions Original instructions...
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Worcestershire, WR14 1XZ United Kingdom. +44 1684 892456 +44 1684 892789 MicroCal, Malvern and the 'hills' logo are registered trademarks in the UK and/or other countries, and are owned by Malvern Instruments Ltd Windows is a registered trademark of Microsoft Corporation...
Replacing the syringe plunger tip ....................Replacing and cleaning the titration syringe ................Troubleshooting ....................How to get help ............................. Troubleshooting chart ........................Reference information ..................Index ........................MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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, and lists of associated documentation. In this chapter This chapter contains the following sections: Section See page 1.1 About this manual 1.2 Important user information 1.3 Regulatory information MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Software items are identified in the text by bold italic text. A colon separates menu levels, thus File:Open refers to the Open command in the File menu. Hardware items are identified in the text by bold text (e.g., Power switch). MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
• You should have a general understanding of the use of a personal computer running Microsoft™ Windows™ in the version provided with your product. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Notes and tips Note: A note is used to indicate information that is important for trouble-free and optimal use of the product. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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1 Introduction 1.2 Important user information Tip: A tip contains useful information that can improve or optimize your procedures. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
This product complies with the European directives listed in the table, by fulfilling the corresponding harmonized standards. A copy of the Declaration of Conformity is available on request. Directive Title 2006/95/EC Low Voltage Directive (LVD) 2004/108/EC Electromagnetic Compatibility (EMC) Directive MicroCal iTC200 System Operating Instructions MAN0558...
CE marked instruments, or • connected to other products recommended or described in the user documentation, • used in the same state as it was delivered from Malvern Instruments, except for alterations described in the user documentation. MicroCal iTC200 System Operating Instructions MAN0558...
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Any equipment connected to MicroCal iTC should meet the safety requirements of EN 61010-1/IEC 61010-1, or relevant harmonized standards. Within EU, connected equipment must be CE marked. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
In this chapter This chapter contains the following sections: Section See page 2.1 Safety precautions 2.2 Labels 2.3 Emergency procedures 2.4 Recycling information 2.5 Declaration of Hazardous Substances (DoHS) MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
• damage to the power cord or its plug • damage caused by dropping the equipment • damage caused by splashing liquid onto it MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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If there is any possibility that liquid may have leaked into the instru- ment case, contact Malvern Instruments immediately. Do not plug the instrument into any electrical outlet until the problem is resolved. WARNING The MicroCal iTC cells are constructed out of Hastelloy™.
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Follow local and/or national regulations for safe operation and maintenance of MicroCal iTC MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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2.1 Safety precautions Installing and moving WARNING MicroCal iTC must be installed and prepared by Malvern Instruments personnel or third party authorized by Malvern Instruments. WARNING Power cord. Only use power cords with approved plugs delivered or approved by Malvern Instruments.
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Do not place containers of liquid on top of the instrument, except those designed for the washing module. Spilled liquid is a fire, electrical and corrosion hazard. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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NOTICE The MicroCal iTC system should always be moved in its normal operating orientation. Other orientations will subject delicate sen- sors inside the instrument to stress. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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WARNING Only spare parts and accessories that are approved or supplied by Malvern Instruments may be used for maintaining or servicing MicroCal iTC MicroCal iTC200 System Operating Instructions MAN0558...
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Contrad 70™ (Decon 90™) is corrosive and therefore dangerous to health. When using hazardous chemicals, avoid spillage and wear protective glasses, gloves, and other suitable personal pro- tective equipment as specified in MSDS. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Labels on the MicroCal iTC The image below shows an example of identification labels attached the rear panel of the MicroCal iTC instrument. Malvern Instruments Malvern Instruments MicroCal iTC200 System Operating Instructions 28-9639-77 AD MicroCal iTC200 System Operating Instructions MAN0558...
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This symbol indicates that the product contains hazardous materials in excess of the limits established by the Chinese standard SJ/T11363-2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronics. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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Warning label specifying the type and rating of fuses for the instrument. Labels showing the company name, product, model number, serial number, and the power supply input for the instrument or accessory. MicroCal iTC200 System Operating Instructions 28-9639-77 AD MicroCal iTC200 System Operating Instructions MAN0558...
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2 Safety instructions 2.2 Labels MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
The data collected up to the time of the power failure is saved. Controller • The controller shuts down. • The MicroCal iTC run is interrupted immediately. Washing module • The washing module shuts down immediately. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Waste electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately. Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Product Maintenance Procedures. This product must not be disposed of as unsorted municipal waste, and must be collected separately and handled properly after decom- missioning. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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SJ/T11363-2006. Data listed in the table represents best information available at the • time of publication 表示该有毒有害物质至少在该部件的某一均质材料中的含量超出 SJ/T11363-2006 标准规定的 限量要求 此表所列数据为发布时所能获得的最佳信息 • MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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MicroCal iTC controller The product has not been tested as per the Chinese standard SJ/T11363-2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronic Information Product. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
This chapter provides a description of MicroCal iTC and an overview of its components. In this chapter This chapter contains the following sections: Section See page 3.1 MicroCal iTC system 3.2 Illustrations MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Data analysis is performed using Origin™ software, wherein the user obtains the stoi- chiometry (n), dissociation constant (K ), and enthalpy (ΔH) of the interaction. The Origin software can also be used to fit more complicated models. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
This section provides illustrations of the MicroCal iTC system. MicroCal iTC system The illustration below shows the MicroCal iTC instrument with the washing module and the controller. Part Description MicroCal iTC Washing module Controller MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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The primary components of MicroCal iTC are illustrated below. Part Description Reagent bottles Loading syringe Cleaning module Washing module Fill port adapter (FPA) Pipette Wash/load station Titrant loading station Calorimeter MicroCal iTC200 System Operating Instructions 28-9639-77 AD MicroCal iTC200 System Operating Instructions MAN0558...
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The illustration below shows the rear of the MicroCal iTC cell unit. Malvern Instruments Malvern Instruments Part Function Main power switch Power fuses IEC 320 inlet power receptacle USB connectors µP activity indicator MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Precautions WARNING MicroCal iTC must be installed and prepared by Malvern Instruments personnel or third party authorized by Malvern Instruments. WARNING Power cord. Only use power cords with approved plugs delivered or approved by Malvern Instruments. WARNING Always plug the instrument into a Ground Fault Circuit Interrupter (GFCI).
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In this chapter This chapter contains the following sections: Section See page 4.1 Site requirements 4.2 Transport 4.3 Hardware connections MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Room temperature fluctuations • Intense sunlight • Vibrations • Strong electrical or magnetic fields (as may be produced by an NMR, microwave oven, large motors or refrigeration units) MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
It is recommended that you test a power conditioner, at your location, before you purchase it. If you believe you are experiencing power source related problems, please contact a Malvern Instruments field engineer. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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Power supply requirements Specification Requirement Voltage Regulation 100-240 VAC Frequency 50/60 Hz Power 70 W Fuses 4.0 A, 250 V, time delay (2 fuses) Protective Earth Terminals Internal/external labeled MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
• Unpack with great care. • Check the equipment for damage before starting assembly and installation. Document any damage and contact your local Malvern Instruments representative. • MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
• Two type A-B (standard) USB connectors Part Description USB 2.0 4-port hub USB Connector type A USB Connector type B (mini) USB Connector type B (standard) MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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35. • The illustration below shows the washing module power supply unit. WARNING Always plug the instrument into a Ground Fault Circuit Interrupter (GFCI). MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
If accidental exposure occurs, flush the affected area with water. If methanol is swallowed, or there is significant skin or eye exposure, seek medical help. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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NOTICE The MicroCal iTC system should always be moved in its normal operating orientation. Other orientations will subject delicate sen- sors inside the instrument to stress. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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5.1 Procedure before an experiment 5.2 Creating a method 5.3 Loading the titration syringe 5.4 Loading the cell 5.5 Performing an experiment 5.6 Procedures after an experiment MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
MicroCal analysis. It is mentioned here, be- software. cause an instance of Origin may be opened during data collection for real time display, though is not necessary. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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Malvern Instruments recommends that the MicroCal iTC application be closed and the master power be turned off, when the system will not be used for extended periods of time. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Creating the method To create the example method, follow the steps described below: Step Action Click on the Advanced Experimental Design tab. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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Enter the following parameters for Injections Setup with the All Same radio button clicked under Edit Mode. Parameter Value Volume (μL) Duration (sec.) Spacing (sec.) Filter Period (sec.) MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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2 Select Unique under Edit Mode. 3 Change the following parameters under Injection Parameters: Parameter Value Volume (μL) Duration (sec.) Click the Save As button and save the method as Training MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Result: The software will prompt the user to move the pipette as necessary, and the Washing Module will fill the syringe. Tip: Avoid over-tightening of the FPA, excessive force can damage the titration syringe. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Finish filling with several small, abrupt spurts of sample to dislodge any bubbles. Lift the tip of the syringe to the point where the cell stem meets the cell port and remove the excess solution. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
User Manual for file naming recommendation). Click the Start button to begin analysis. Once a titration run starts, the real-time data is displayed in the Real Time Plot tab. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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5 Operation 5.5 Performing an experiment Note: Proper sample preparation is described in the MicroCal iTC system User Manual and will not be described here. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Move the pipette and firmly seat into the wash and dry station. Insert the cell cleaning module into the sample cell. Be sure to push down until the module is firmly seated. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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Remove the cell cleaning module and disconnect the FPA if all experiments are completed. Fill the sample cell with clean, distilled water. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
User Manual may be performed by the user. When performing any of those procedures, be mindful of the cautionary remarks displayed below. In addition, annual preventive maintenance should be performed by a trained Malvern Instruments service technician. Precautions WARNING...
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6 Maintenance WARNING Only spare parts and accessories that are approved or supplied by Malvern Instruments may be used for maintaining or servicing MicroCal iTC WARNING Disconnect power. Always disconnect power from the instrument before replacing any component on the instrument, unless stated otherwise in the user documentation.
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This chapter contains the following sections: Section See page 6.1 Cleaning the cell 6.2 Refilling the reference cell 6.3 Replacing the syringe plunger tip 6.4 Replacing and cleaning the titration syringe MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
WARNING Always use appropriate personal protective equipment (PPE) during cleaning and maintenance of the equipment. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
ITC Method. Procedure To refill the reference cell, follow the steps described in Section 5.4 Loading the cell, on page 53. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
To remove the syringe plunger tip, follow the steps described below: Step Action Place the pipette assembly in the (empty) titrant load location. Loosen and remove the securing nut from bottom of the pipette. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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In the MicroCal iTC software, click on the Instrument Controls tab and click 1. Remove Old Tip in the Maintenance section. The software prompts to remove the old tip. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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Grab the syringe bore firmly and pull straight down to remove the syringe from the pipette. The soft-grip tweezers can be used to help grip the syringe without damaging it. Set the syringe aside. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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Insert the tip puller into the pipette until the tip of the puller grabs the plunger tip. Gently extract the tip puller (along with the plunger tip) from the pipette. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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Insert a new plunger tip inside the tip pusher tool. The tip pusher tool has a hole on one side. Insert the plunger tip inside that hole with the plunger tip hole (1) facing outward. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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Do not push too hard. Resistance should be felt initially. Reinstalling the titration syringe To reinstall the syringe, follow the steps described in Installing the titration syringe, on page 71. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Always examine the fill port adaptor closely following the identification of a damaged syringe. • If the syringe will continue to be used, clean the top section carefully with the syringe brush. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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It may take a few attempts to thread the wire into the channel. Rinse the syringe well. Replace the syringe if any material remains in the syringe that cannot be removed by this method. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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The top portion of the syringe is keyed to slide into the pipette in only one way. Reinstallation procedure To insert a titration syringe, follow the steps described below: MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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Keep the small metal pin facing forward and centered in the opening in the pipette while sliding the syringe up and into the pipette. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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If it seats directly then there will be about 2 mm of glass exposed and the pipette and syringe can no longer be moved independently. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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When the syringe is properly seated, the syringe and the syringe holder in the pipette are locked together. The syringe is not properly seated if the syringe can spin without spinning the syringe holder. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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Replace and tighten the bottom nut. Be careful not to bend or otherwise damage the paddle when reinstalling the bottom nut. The bottom nut should be snug but easily removed. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
This section contains tips and information for troubleshooting the MicroCal iTC system. In this chapter This chapter contains the following sections: Section See page 7.1 How to get help 7.2 Troubleshooting chart MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Some problems can easily be corrected by the average user; some may be corrected by the more advanced user, and some require the expertise of a Malvern Instruments service technician. The Malvern Instruments service department is happy to provide any advice, parts or service that may be necessary.
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The water runs should be provided to the MicroCal service technician for evaluation. Following the evaluation, a representative from the service department will contact you with comments and recom- mendations. MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Section 7.1 How to get help, on page 77 Titration syringe is damaged See Section 6.4 Replacing and cleaning the titration syringe, on page 69 for replacement MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Physical specifications Property Value Cell material Hastelloy Alloy C-276 Weight (fully assembled): 9.4 kg Dimensions: Calorimeter (W x H x D) 21 × 33.7 × 34.9 cm MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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These include the clearances. Service functions will require an additional 31 cm (12 in.) overhead clearance. Clearance ≥ 15 cm (6 in.) behind the Calorimeter, • ≥ 40 cm (15 in.) in front. • MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
Storage (no liquid in cells): Temperature -25°C to 60°C (no liquid in cells) Humidity 10% to 90%, non-condensing Atmospheric pressure 500 to 1060 hPa) Additional information For additional information, visit: www.malvern.com/microcal MicroCal iTC200 System Operating Instructions MAN0558 MicroCal iTC200 System Operating Instructions 28-9639-77 AD...
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