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Holbach MBASS30V3 Operating Manual

Microbiological air sampling system
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Mikrobiologisches Luftprobenahmesystem
MBASS30
V3
Bedienungsanleitung
Operating Manual

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Summary of Contents for Holbach MBASS30V3

  • Page 1 Mikrobiologisches Luftprobenahmesystem MBASS30 Bedienungsanleitung Operating Manual...
  • Page 2 The rubbish bin symbol stands for: batteries and rechargeable batteries may not be disposed of in household waste. The batteries in this device do not contain lead, cadmium or mercury. Umweltanalytik Holbach GmbH Sperberweg 3, DE-66687 Wadern Phone: +49 (0) 6874 / 182277...

  • Page 3: Table Of Contents

    Air Sampling System MBASS30 Contents Contents Section Page Introduction The Air Sampling System MBASS30 The Features of MBASS30 Package Contents Operating Elements Connection Sockets Charger Operating MBASS30 Configuring Presettings Sampling History Configuring MBASS30 (Setup) The Sampling Heads for MBASS30 The Coated Slide Information Service Functions Cleaning and Maintenance Instructions...

  • Page 4: Introduction

    Air Sampling System MBASS30 Introduction Thank you very much for choosing our air sampling system MBASS30 Please read this manual and the safety instructions carefully before using the device. , including the sampling heads and other accessories, is a laboratory device and may only be used by trained personnel. MBASS30 may only be used for the purposes described in this operating manual.

  • Page 5: The Air Sampling System Mbass30

    Air Sampling System MBASS30 1. The Air Sampling System MBASS30 The MicroBiological Air Sampling System MBASS30 has been developed for different sampling methods: Microbiological air sampling with the sampling heads LKS100 and LKS 30 for loading of culture media in 90 mm standard petri dishes for cultivat- ing.

  • Page 6: The Features Of Mbass30 V3

    Air Sampling System MBASS30 2. The Features of MBASS30 Figure 1 with sampling head LKS100 MBASS30 10 individual presettings (description text, volume, volume flow, start delay, logging interval of the sampling process) Volume flow can be preset in the range from 30 to 100 l/min Sampling volume can be preset from 10 liters to 10,000 liters Start delay from 1 second to 24 hours 4.3 inch (109 mm) color display with additional glass cover...

  • Page 7: Package Contents

    Air Sampling System MBASS30 3. Package Contents 3. Package Contents The delivery of the microbiological air sampling system contains the following: MBASS30 the robust MBASS30 base device quick-charger for operation at 100 - 240 V, 50 - 60 Hz this operating manual a USB connection cable for connection to a PC the software MBASSControl for configuration, remote control and adjustment of MBASS30...

  • Page 8: Operating Elements

    Air Sampling System MBASS30 4. Operating Elements 4. Operating Elements 4.1 Overview of the Operating Elements Locking springs for the sampling heads 4.3 inch color display with touchpanel Carrying handle Figure 2 MBASS30 Locking springs for the sampling heads Outlet for the sampling air On/Off button Release...
  • Page 9 Air Sampling System MBASS30 4. Operating Elements 4.2 Carrying Handle The handle for comfortable carrying of MBASS30 locks into place every 30 degrees. To adjust the handle, please push both metallic re- lease buttons (violet arrows in figure 3) at once. Rotate the handle into the desired position and let go the release buttons.
  • Page 10 Air Sampling System MBASS30 4. Operating Elements 4.6 Tripod Sockets There are two tripod sockets in the MBASS30 device base: UNC 1/4 inch (photo) and UNC 3/8 inch (microphone) Only use tripods that can support the weight of MBASS30 (approx. 3 kg) and ensure that they provide a secure standing.
  • Page 11 Air Sampling System MBASS30 4. Operating Elements Centering pin Position marks Recessed grips Settings disc Centering pin Centering pin Figure 5 Centering mechanism wide open, disc in position 0 Figure 6 Centering mechanism narrow, disc in position 12 Operating Manual MBASS30 LKS100 LKS 30 PS 30 FA 30...

  • Page 12: Connection Sockets

    Air Sampling System MBASS30 5. Connection Sockets 5. Connection Sockets In the back of MBASS30 there are 4 connection sockets: 5.1 Battery Charger Socket Connect the charger plug of the delivered quick-charger into the round battery charger socket (low voltage plug EIAJ5). Only use the charger that is included in the delivery for charging.
  • Page 13 Air Sampling System MBASS30 5. Connection Sockets Figure 7 The temperature/humidity sensor rotronic HC2A-S 5.3 USB Socket H A FAT formatted memory stick can be connected to the USB socket H (host). Sampling data can be exported in CSV format from the history view to this memory stick.

  • Page 14: Charger

    Air Sampling System MBASS30 6. Charger 6. Charger A quick-charger for charging the integrated Lithium-Polymer battery pack (14.4 Volt / 3800 mAh) is part of the delivery of MBASS30 Status indicator Figure 10 The quick-charger for MBASS30 The charger can be operated at a voltage of 100 - 240 volts and a mains frequency of 50 - 60 Hz.
  • Page 15 Air Sampling System MBASS30 6. Charger Manufacturer logo Figure 11 The rear of the charger contains the status colors, certification mark and manufacturer logo Note: The charger can also be used as power supply. The available current is greater than the current required for the operation of MBASS30 Please note the safety instructions in section 16! Operating Manual MBASS30...

  • Page 16: Operating Mbass30

    Air Sampling System MBASS30 7. Operating MBASS30 7. Operating MBASS30 Operating MBASS30 is simple and consists of the following steps: Prior to sampling Minimum distance of Place MBASS30 safely rear to a wall: 10 cm Switch on MBASS30 Section 7.1 Only for temperature differences of >...
  • Page 17 Air Sampling System MBASS30 7. Operating MBASS30 7.1 Switching on MBASS30 To switch on MBASS30 , press the on/off button on the back of the device (Figure 3). When switching on, two short signal tones will sound and the display shows the personalization text, the firmware version and the device’s serial number for 5 seconds: Personalization Figure 12 Display when switching on...
  • Page 18 Air Sampling System MBASS30 7. Operating MBASS30 Each preset program contains: a description text (editable using the MBASSControl software) the sampling volume in liters, range: 10 up to 10,000 l the volume flow in liters per minute, range: 30 up to 100 l/min the start delay, range: 1 second up to 24 hours the logging interval in seconds, range: 0 up to 240 s 7.2 Selecting a Preset Program...
  • Page 19 Air Sampling System MBASS30 7. Operating MBASS30 7.3 Starting the Sampling Prior to sampling, the corresponding sampling head has to be prepared and the sampling medium is to be inserted. Please note the following sections describing the sampling heads LKS100 / LKS 30 (Sec- tion 11.1), PS 30 (Section 11.2) and FA 30 (Section 11.3) for this.
  • Page 20 Air Sampling System MBASS30 7. Operating MBASS30 If a start delay is configured, the sampling starts after this delay. While this time is running, the remaining delay time is displayed instead of the remaining time of the sampling. Indicator fo the current delay until start Figure 17...
  • Page 21 Air Sampling System MBASS30 7. Operating MBASS30 Note: While the delay time is running and during a pause, the (climate) data is still being recorded, if the logging of the sampling process is enabled. 7.5 Finishing the Sampling When the target sampling volume is reached, MBASS30 shows a message box in the display, plays a 3-tone sequence and stops the sampling.
  • Page 22 Air Sampling System MBASS30 7. Operating MBASS30 Figure 20 Dialog to confirm the abortion of a sampling 7.7 Sampling Disruptions The following errors are detected and displayed by MBASS30 dur- ing sampling: Low battery voltage The air flow is blocked. The preselected volume flow cannot be reached A sensor fault (pressure or temperature) has occurred The fan motor is faulty...
  • Page 23 Air Sampling System MBASS30 7. Operating MBASS30 A hint on how to resolve the error can be displayed by tapping the button. The error can be acknowledged by tapping the button. As long as an error is present, no new samplings can be started. In that case, the button will be displayed instead of the button...
  • Page 24 Air Sampling System MBASS30 7. Operating MBASS30 Note: When the on/off button on the back of the device (Figure 3) is pressed for more than 10 seconds, a reset of the internal microcontroller is per- formed. 7.9 Battery Voltage too Low If the battery voltage falls below 13 Volts, a running sampling will be aborted and acoustic and visual signals will be given.

  • Page 25: Configuring Presettings

    Air Sampling System MBASS30 8. Configuring Presettings 8. Configuring Presettings 8.1 Selecting Presettings To configure and modify the 10 available sampling presets (programs), the respective program has to be selected first by tapping on the but- tons on the left and right of the screen (Figure 14). In the following example, the preset (program no.
  • Page 26 Air Sampling System MBASS30 8. Configuring Presettings 8.2 Editing the Presettings After tapping the pencil symbol , the volume can be modified. Figure 26 The editor shows the current value The current value is cleared by tapping the button. Afterwards, the new value can be entered.
  • Page 27 Air Sampling System MBASS30 8. Configuring Presettings To change the start delay, the input mask for time entries is shown by tapping the corresponding pencil symbol Figure 29 The editor for time entries Time entries have to be input in the format Hours:Minutes:Seconds. The new value is accepted by tapping the button.
  • Page 28 Air Sampling System MBASS30 8. Configuring Presettings Figure 31 Selection mask for the volume flow 8.3 Saving the Presettings The changes to the presettings are saved by tapping the button in the main edit mode screen (Figure 25). Tapping the button discards the changes.
  • Page 29 Air Sampling System MBASS30 8. Configuring Presettings The start delay can be set to the second in the range from 0 seconds (no start delay) up to 24 hours. 8.4.6 Logging interval The sampling process can be logged in the set interval. Apart from the volume flow, the climate parameters are also recorded.

  • Page 30: Sampling History

    Air Sampling System MBASS30 9. Sampling History 9. Sampling History The data of the recent 100 samplings is stored for displaying in a ring- buffer. A sequential sampling number in the range from 0 to 65535 is assigned to each sampling. The history data contains: Sampling number Start operation (date and time)
  • Page 31 Air Sampling System MBASS30 9. Sampling History The two buttons on the sides can be used to select the other entries. (Figure 32). 9.2 Exporting History Data By tapping the button, the currently displayed data can be ex- Export ported in CSV format to a connected USB memory stick into the direc- tory “\sampling_data”.

  • Page 32: Configuring Mbass30 (Setup)

    Air Sampling System MBASS30 10. Configuring MBASS30 10. Configuring MBASS30 10.1 Device Settings All configuration settings can be changed directly on MBASS30 also using the MBASSControl software. By tapping the button on the main screen (Figure 13), the set- Settings tings menu is displayed: Figure 34 The settings menu...
  • Page 33 Air Sampling System MBASS30 10. Configuring MBASS30 The other settings can be changed by tapping the pencil button One of four different input masks will be shown: 1. Slider Tap and move the blue knob to change the value. Save with button OK, Discard with button Cancel.
  • Page 34 Air Sampling System MBASS30 10. Configuring MBASS30 10.2 Sampling Settings In the sampling settings, the number of displayed presettings (programs) can be set in the range from 1 to 10. To change the value, the input field has to be cleared first with the button.

  • Page 35: The Sampling Heads For Mbass30

    Air Sampling System MBASS30 11. The Sampling Heads for MBASS30 11. The Sampling Heads for MBASS30 The preparation of the sampling heads, except for AS100, is described in this section. 11.1 Air Samplers LKS100 / LKS 30 For air sampling, the two efficient sampling heads LKS100 with a vol- ume flow of 100 l/min and LKS 30 with a volume flow of 30 l/min are available.
  • Page 36 Air Sampling System MBASS30 11. The Sampling Heads for MBASS30 Spores Culture medium Figure 36 Functional principle of the air samplers Note: The following describes the sampling process for the LKS100 air sampler. Sampling with the LKS 30 air sampler is identical with the exception that it is operated with a volume flow rate of 30 l/min.
  • Page 37 Air Sampling System MBASS30 11. The Sampling Heads for MBASS30 Figure 39 Cleaning the middle part Figure 40 Taking off the sampling head After the cleaning agent has dried, LKS100 can be assembled again. Place a closed petri dish with culture media in the center of the setting disk.
  • Page 38 Air Sampling System MBASS30 11. The Sampling Heads for MBASS30 11.1.3 After sampling Open the locking mechanism. Lift the top and middle parts from the base and close the petri dish with its lid. Remove the petri dish from the sampler and put the middle and top parts back to the base.
  • Page 39 Air Sampling System MBASS30 11. The Sampling Heads for MBASS30 11.2 Particle Sampler PS 30 The particle sampler PS 30: samples spores, pollen, bacteria, fibers and other airborne micro- particles on adhesively coated standard slides (76 mm x 26 mm x 1 mm) permits microscopic analysis of all micro-organisms (including those that can not be cultivated) immediately after sampling fully utilizes the coating with three parallel samplings (no.
  • Page 40 Air Sampling System MBASS30 11. The Sampling Heads for MBASS30 11.2.2 Preparation for Sampling Open the centering mechanism of MBASS30 by turning the setting disk counter-clockwise (Section 4.7). Clean the individual components (slide mount and top part) using a ger- micidal and fungicidal agent (e.g.
  • Page 41 Air Sampling System MBASS30 11. The Sampling Heads for MBASS30 Remove the protective cap from the top of PS 30. Place the top part of PS 30 on MBASS30 in such a way, that the sample numbers on the top part match the sample numbers of the slide mount.
  • Page 42 Air Sampling System MBASS30 11. The Sampling Heads for MBASS30 Figure 44 Completely overloaded particle tracks (cannot be evaluated) 11.2.4 After Sampling Another sampling can take place after moving the slot jet to the next position. 11.2.5 Removing the Slide After all samples have been taken on the inserted slide, remove the loaded slide in the following sequence: Open the sampler by lightly pushing the locking springs at the black...
  • Page 43 Air Sampling System MBASS30 11. The Sampling Heads for MBASS30 11.3 The Filter Adapter FA 30 The filter adapter FA 30: samples spores, pollen, bacteria, fibers and other airborne microparticles by filtration using round 80 mm gelatine filters in single-use units is suited for air sampling and evaluation according to the BIA ) worksheet 9420, Berufsgenossenschaftliches Institut für Arbeitsschutz...
  • Page 44 Air Sampling System MBASS30 11. The Sampling Heads for MBASS30 11.3.2 After Sampling Remove the filter with the single-use unit and store it according to the (handling) instructions of the manufacturer. 11.3.3 Notes Avoid wedging FA 30 and MBASS30 when installing. Clean FA 30 before and after each use.

  • Page 45: The Coated Slide

    We recommend the adhesively coated slides for PS 30. Dimensions: 76 mm x 26 mm (Article no.: 02-155). Probe-Nr. / Sample no. Spur Spur www.holbach.biz Art. Nr.: 02-155 Track Track Datum / Date Figure 46 Coated slide 76 mm x 26 mm x 1 mm...
  • Page 46 Air Sampling System MBASS30 12. The Coated Slide 12.1 The Shipping Container The adhesively coated slide is delivered in a shipping container which is sealed in a film tubing. After taking the shipping con- tainer out of the film tubing, it can be opened by lifting the cap on one side.

  • Page 47: Information

    Air Sampling System MBASS30 13. Information 13. Information stores information on the operating time, the drawn MBASS30 volume, etc. This information is shown under the menu item in the Info settings menu. Figure 50 Example for the first information page Figure 51 Example for the second information page Tap the...

  • Page 48: Service Functions

    Air Sampling System MBASS30 14. Service Functions 14. Service Functions Three service functions are implemented in MBASS30 14.1 Fan This service function allows to manually control the fan to test the func- tion of the fan and the difference pressure sensor. Tap the but- Service ton in the settings menu (Figure 34).
  • Page 49 Air Sampling System MBASS30 14. Service Functions To invoke the function, tap the button in the service Tightness Test menu. Close the inlet of the sampling head and start the test with the button. The fan will be run for 15 seconds. The maximum and the average of the difference pressure at the measuring orifice is measured.

  • Page 50: Cleaning And Maintenance Instructions

    Air Sampling System MBASS30 15. Cleaning and Maintenance Instructions 15. Cleaning and Maintenance Instructions Only clean MBASS30 with a damp and lint-free cloth. Do not use abrasives. Do not exert pressure on the display glass. 15.1 Calibration Interval The recommended calibration and maintenance interval is at every 150 operating hours of the high-performance fan or at least every 2 years.

  • Page 51: Safety Instructions

    Air Sampling System MBASS30 16. Safety Instructions 16. Safety Instructions Non-compliance with these safety instructions may lead to damages of the device and even to dangerous personal injury! may only be used for the intended purpose! MBASS30 may only be used by qualified personnel! MBASS30 Prevent fluids from entering MBASS30 and the charger!

  • Page 52: Technical Data

    Air Sampling System MBASS30 17. Technical Data 17. Technical Data Sampling heads: Round jet impactor LKS100, Round jet impactor LKS 30, Slot jet impactor PS 30, filter adapter FA 30 and allergen sampling head AS100 Volume flow: 30 l/min up to 100 l/min, controlled Tolerance: ±...
  • Page 53 Air Sampling System MBASS30 17. Technical Data Sampling Head Air Sampler LKS 30: Sample air volume: 50 up to 400 l, depending on the task Volume flow: 30 l/min Validation: according to EN ISO 14698-1:2003 Vacuum: approx. 0.1 bar at 30 l/min volume flow Operating position: arbitrary during sampling Sampling medium:...
  • Page 54 Air Sampling System MBASS30 17. Technical Data Sampling Head Particle Sampler PS 30: Sample air volume: 200 l (recommended for usual contamination) Also note the recommendations in Section 11.2.3 Volume flow: 30 l/min Slot jet dimensions: 16.0 mm x 1.1 mm Operating position: arbitrary during sampling Method:...

  • Page 55: Conditions Of Warranty

    We reserve the right to repair or replace. The parts used for this are new or as good as new. Returned parts become property of Umwelt- analytik Holbach GmbH. A repair under warranty does not extend the warranty of the parts or the products itself. Excluded from warranty are...

  • Page 56: The Mbasscontrol Software

    Air Sampling System MBASS30 19. The MBASSControl Software 19. The MBASSControl Software Delivery includes the MBASSControl software, compatible to Microsoft Windows 7 and above, on a data medium. is connected with the USB socket. MBASS30 19.1 Overview The MBASSControl software can be used to: remotely control MBASS30 configure MBASS30 change the presettings (programs)
  • Page 57 Air Sampling System MBASS30 19. The MBASSControl Software 19.2 Installation of MBASSControl The installation of MBASSControl is done by executing the installation program MBASSControl_Setup. Start MBASSControl_Setup by double-clicking and follow the installa- tion instructions. When the software is installed for the first time on a system, please make sure that the USB drivers are installed as well.

  • Page 58: Statistical Correction

    Air Sampling System MBASS30 Appendix A: Statistical Correction of the CFU Why statistical correction of the colony forming units (CFU)? With round jet impactors, the particles hit the so-called germination spot below the jet hole and germinate there. The visible colonies are counted and projected to a standard of 1 m³...
  • Page 59 Air Sampling System MBASS30 Appendix A: Statistical Correction of the CFU On 1: Depending on the spore concentration in the air, more than one spore per jet hole is will be accelerated and will hit the corresponding germination spot during a sampling. The more the germination spots are filled, the lower the probability for a spore to hit a spot that is not occupied and still “available”.
  • Page 60 Air Sampling System MBASS30 Appendix A: Statistical Correction of the CFU Corrected values Number of CFU Limits of the statistical correction A correction of the number of CFUs with the number of particles deter- mined statistically is possible only on the total spore count. A projection of the CFU numbers with the individual identified species of the CFU is not possible with this statistical procedure.

  • Page 61: Appendix B Correction Table For Lks 30

    Air Sampling System MBASS30 Appendix B: Table for Statistical Corr. of LKS 30 Corr Corr Corr Corr Corr Corr Operating Manual MBASS30 LKS100 LKS 30 PS 30 FA 30...

  • Page 62: Appendix C Correction Table For Lks100

    Air Sampling System MBASS30 Appendix C: Table for Statistical Corr. of LKS100 Corr Corr Corr Corr Corr Corr Corr Corr Corr Corr Operating Manual MBASS30 LKS100 LKS 30 PS 30 FA 30...

  • Page 63: Appendix D Troubleshooting

    Air Sampling System MBASS30 Appendix D: Troubleshooting This appendix lists possible MBASS30 faults along with cor- rective solutions. Fault Possible solution Charge battery, cannot be switched on. press the on/off button for more than MBASS30 10 seconds (reset will be performed), have the fuses in the device checked does not Check the settings whether the auto-...
  • Page 64 Air Sampling System MBASS30 Appendix D: Troubleshooting Error messages after power-on and when idle (continued) Error message Possible solution Temperature sensor The integrated temperature sensor is faulty faulty. Use an external temperature sensor. Send in for repair. Error messages during a sampling Error message Possible solution Motor failure!

  • Page 65: Declaration Of Ce Conformity

    Air Sampling System MBASS30 Appendix E: Declaration of Conformity EG – Konformitätserklärung DECLARATION OF CONFORMITY (DoC) Mikrobiologisches Probenahmesystem MBASS30 Umweltanalytik Holbach GmbH Name und Anschrift des Herstellers Sperberweg 3 Name and address of the manufacturer MBASS30 66687 Wadern Germany +49 (0)6874/182277...

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