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Beckman Couler UniCel DxC 600 In-Lab Training Manual

Synchron clinical systems
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A24183-AA
May 2005
®
In-Lab Training Manual
This manual is intended for use with
®
UniCel
DxC 600 and DxC 800
®
Synchron
Clinical Systems
© Copyright 2005 Beckman Coulter, Inc.
BECKMAN COULTER, INC. • 4300 N. Harbor Blvd., Fullerton, CA 92835 U.S.A.

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  • Page 1 A24183-AA May 2005 ® In-Lab Training Manual This manual is intended for use with ® UniCel DxC 600 and DxC 800 ® Synchron Clinical Systems © Copyright 2005 Beckman Coulter, Inc. BECKMAN COULTER, INC. • 4300 N. Harbor Blvd., Fullerton, CA 92835 U.S.A.

  • Page 3: Table Of Contents

    Procedures and Processes ................3-1 Daily Start Up Procedures ................3-1 Check Status and Clear Sample Racks ............3-2 When to Do ....................3-2 Result ......................3-2 UniCel DxC 600/800 In-Lab Training Manual A24183 Table of Contents May 2005 Page 1 of 4...
  • Page 4 Quick Tips ....................3-18 Additional References ................3-18 Procedure ....................3-18 Programming and Processing Patient Samples Manually ......3-19 When to Do ....................3-19 Result ....................... 3-19 Table of Contents UniCel DxC 600/800 In-Lab Training Manual A24183 Page 2 of 4 May 2005...
  • Page 5 Instrument Commands ................4-8 Answer Key ....................4-9 Reagents ..................... 4-9 Calibration ....................4-10 Samples ....................4-10 UDR ......................4-10 Instrument Commands ................4-10 UniCel DxC 600/800 In-Lab Training Manual A24183 Table of Contents May 2005 Page 3 of 4...
  • Page 6 Table of Contents UniCel DxC 600/800 In-Lab Training Manual A24183 Page 4 of 4 May 2005...

  • Page 7: Chapter 1 System Components

    DxC 800 A007386P.EPS Figure 1.1 DxC 600/800 Analyzers System Components Both UniCel DxC 600 and DxC 800 can be divided into the following components: • Sample Handling Components, • Modular Chemistry (MC) System, • Cartridge Chemistry (CC) System, • Hydropneumatic System, •...

  • Page 8: Sample Handling Components

    1. Pushers 5. Sample gate 2. Autoloader 6. Shuttle 3. Run button 7. Offload track 4. Priority load button 8. Stop button Figure 1.2 Sample Loading Area System Components UniCel DxC 600/800 In-Lab Training Manual A24183 Page 1-2 May 2005...

  • Page 9: Sample Racks

    The STOP button is located near the Off Load Tray and it is used to mechanically stop all instrument movements. UniCel DxC 600/800 In-Lab Training Manual A24183 System Components May 2005 Page 1-3...

  • Page 10: Pushers Gate And Shuttle

    Home position. The interior of the probe is rinsed and the waste is collected while at the Home position. System Components UniCel DxC 600/800 In-Lab Training Manual A24183 Page 1-4 May 2005...

  • Page 11: Obstruction Detection Assembly

    Detailed information regarding the Obstruction Detection Assembly can be found in the UniCel DxC Synchron Clinical Systems Instructions For Use manual, CHAPTER 2, System Description. UniCel DxC 600/800 In-Lab Training Manual A24183 System Components May 2005 Page 1-5...

  • Page 12: Modular Chemistry (Mc) System

    DxC 600 systems have one-cup module for the rapid Module(s) analysis of glucose. DxC 800 systems have 6 individual cup modules for the rapid analysis of glucose, BUN/UREA, creatinine, phosphorus, albumin and total protein. System Components UniCel DxC 600/800 In-Lab Training Manual A24183 Page 1-6 May 2005...
  • Page 13 3. PHOSm (DxC 800 only) 7. ALBm (DxC 800 only) 4. GLUCm Figure 1.4 DxC 800 Modular Chemistry System A007388P.EPS Figure 1.5 DxC 600 Flow Cell Assembly UniCel DxC 600/800 In-Lab Training Manual A24183 System Components May 2005 Page 1-7...

  • Page 14: Electrolyte Injection Cup (Eic)

    The EIC mixes the sample with Electrolyte Buffer reagent prior to the delivery (of sample) to the flow cell. 1. Waste Outlet 4. Reference Inlet 2. Flow Cell Outlet 5. DI H 0 Inlet 3. Buffer Inlet Figure 1.6 System Components UniCel DxC 600/800 In-Lab Training Manual A24183 Page 1-8 May 2005...

  • Page 15: Flow Cell Assembly

    • Stir bar and mixer motor assembly - Effectively mixes the contents of the reaction cup • A detection system - (Chemistry dependent) serves to detect light transmitted or electrical changes UniCel DxC 600/800 In-Lab Training Manual A24183 System Components May 2005 Page 1-9...

  • Page 16: Cartridge Chemistry (Cc) System

    (Optional) Note: Also known as Near Infrared Particle Immuno Assay (NIPIA). Cuvette Wash Station Washes cuvettes at the completion of analysis in preparation for the next chemistry. System Components UniCel DxC 600/800 In-Lab Training Manual A24183 Page 1-10 May 2005...

  • Page 17: Reagent Cartridges

    Both reagent probes on the UniCel DxC work in tandem to provide maximum throughput and efficient reagent delivery. UniCel DxC 600/800 In-Lab Training Manual A24183 System Components May 2005...

  • Page 18: Reaction Carousel

    Photometer assembly. Additional information about the LPIA assembly may be found in the UniCel DxC Synchron Clinical Systems Instructions For Use manual, CHAPTER 2, System Description, Cuvette Reaction System. System Components UniCel DxC 600/800 In-Lab Training Manual A24183 Page 1-12 May 2005...

  • Page 19: Cuvette Wash Station

    Water. The last probe removes DI water from the cuvette, leaving it dry and ready for the next chemistry. 1. Probe #1 4. Probe #4 2. Probe #2 5. Wash station 3. Probe #3 Figure 1.10 Cuvette Wash Station UniCel DxC 600/800 In-Lab Training Manual A24183 System Components May 2005 Page 1-13...

  • Page 20: Hydropneumatics System

    1. Wash concentrate solution 4. Dilute wash solution reservoir 2. No foam reagent 5. Wash concentrate reservoir 3. DI water reservoir 6. Auto-Gloss Figure 1.11 DxC Hydropneumatics System Components UniCel DxC 600/800 In-Lab Training Manual A24183 Page 1-14 May 2005...

  • Page 21: System Console

    DxC System. For detailed information on the operation of these components, please refer to the individual console computer, monitor or printer’s Operations Manual. UniCel DxC 600/800 In-Lab Training Manual A24183 System Components May 2005...
  • Page 22 System Console Cuvette Wash Station System Components UniCel DxC 600/800 In-Lab Training Manual A24183 Page 1-16 May 2005...

  • Page 23: Chapter 2 Software Overview

    5. Sample Status Icons 2. Host Indicator 6. Rack Status 3. System Status Indicator 7. Function Bar 4. Function Select Icons Figure 2.1 UniCel DxC Main Operator Screen UniCel DxC 600/800 In-Lab Training Manual A24183 Software Overview May 2005 Page 2-1...

  • Page 24: Main Operator Screen

    These icons provide access to each of the major functional areas of the system. Table 2.1 Function Select Icons Icon Functional Area Main Screen Sample Programming Results Recall Reagents/Calibration Set Up Utilities Status Instrument Commands (1 of 2) Software Overview UniCel DxC 600/800 In-Lab Training Manual A24183 Page 2-2 May 2005...

  • Page 25: Sample Status Icons

    Carousel. As each rack is loaded, the rack number is displayed at the top of the Rack Status box. All samples on the rack are listed below the rack number. UniCel DxC 600/800 In-Lab Training Manual A24183 Software Overview May 2005...
  • Page 26 Main Operator Screen Rack Status Software Overview UniCel DxC 600/800 In-Lab Training Manual A24183 Page 2-4 May 2005...

  • Page 27: Chapter 3 Procedures And Processes

    • Program (if necessary) and process controls. • Review control results to verify system operation. • Program (if necessary) and process patient samples. • Review patient results. UniCel DxC 600/800 In-Lab Training Manual A24183 Procedures and Processes May 2005 Page 3-1...

  • Page 28: Check Status And Clear Sample Racks

    For more information about this procedure, refer to the UniCel DxC Synchron Clinical Systems Instructions For Use manual, CHAPTER 3, Preparing Samples for Analysis, How to Use Reserved Racks, Clearing Samples. Procedures and Processes UniCel DxC 600/800 In-Lab Training Manual A24183 Page 3-2 May 2005...

  • Page 29: Procedure

    Highlight the Racks(s) Field and type the rack number(s) to be cleared. Select <OK> to clear the racks. Select <OK> to confirm the rack(s) to be cleared. UniCel DxC 600/800 In-Lab Training Manual A24183 Procedures and Processes May 2005 Page 3-3...

  • Page 30: Check Status And Reagent Load Of Mc Reagents

    • Adequate # of tests remaining for CC reagents. • Reagents do not have a reagent status of "Reagent Expired" or "Days Exceeded." Unload/Load reagents as indicated. Procedures and Processes UniCel DxC 600/800 In-Lab Training Manual A24183 Page 3-4 May 2005...
  • Page 31 • Load the new reagent bottle in the MC Bulk Reagent Compartment. Repeat Steps 5–7/8 for unloading and loading of additional MC reagents. Select Done [F10]. Before processing patient samples, calibrate and run QC. UniCel DxC 600/800 In-Lab Training Manual A24183 Procedures and Processes May 2005 Page 3-5...

  • Page 32: Check Status And Reagent Load Of Cc Reagents

    • UniCel DxC Synchron Clinical Systems Instructions For Use manual, CHAPTER 5, Reagent Load/Calibration. • SYNCHRON Clinical Systems Chemistry Information Manual, refer to chemistry specific Chemistry Information Sheet. • Reagent Preparation Quick Reference. Procedures and Processes UniCel DxC 600/800 In-Lab Training Manual A24183 Page 3-6 May 2005...

  • Page 33: Procedure

    Clear [F1]. • Do not close the reagent carousel door. Continue with the next step to load a reagent. (1 of 2) UniCel DxC 600/800 In-Lab Training Manual A24183 Procedures and Processes May 2005 Page 3-7...
  • Page 34 If multiple positions were selected for unloading/loading, repeat Step 5 and Step 6. Before processing patient samples, calibrate and run QC if needed. (2 of 2) Procedures and Processes UniCel DxC 600/800 In-Lab Training Manual A24183 Page 3-8 May 2005...

  • Page 35: Calibration

    Select the chemistries to be calibrated. Use the <Page Up> and <Page Down> buttons to select more chemistries. Select Cal [F4]. Select List [F5] to review the Calibrator Load List. (1 of 2) UniCel DxC 600/800 In-Lab Training Manual A24183 Procedures and Processes May 2005 Page 3-9...
  • Page 36 Place the rack(s) in the autoloader with the rack’s bar code label to the right. Press the RUN button on the analyzer. (2 of 2) Procedures and Processes UniCel DxC 600/800 In-Lab Training Manual A24183 Page 3-10 May 2005...

  • Page 37: Sample Preparation And Sample Containers

    For more information about this procedure, refer to the UniCel DxC Synchron Clinical Systems Instructions For Use manual, CHAPTER 3, Preparing Samples for Analysis. All trademarks are the property of their respective owners. UniCel DxC 600/800 In-Lab Training Manual A24183 Procedures and Processes May 2005...

  • Page 38: Procedure

    • Remove the cap, if present. • Determine if volume is adequate. • Check for fibrin. • Ensure that container is placed in a reserved or non-reserved rack. (1 of 3) Procedures and Processes UniCel DxC 600/800 In-Lab Training Manual A24183 Page 3-12 May 2005...
  • Page 39 Unreadable Bar Code Patient Samples" damaged and cannot be in this manual. replaced Not programmed • Go to "Programming and Processing Patient Samples Manually" in this manual. (2 of 3) UniCel DxC 600/800 In-Lab Training Manual A24183 Procedures and Processes May 2005 Page 3-13...
  • Page 40 A014600L.EPS Figure 3.1 Bar Code Label Placement (3 of 3) All trademarks are the property of their respective owners. Procedures and Processes UniCel DxC 600/800 In-Lab Training Manual A24183 Page 3-14 May 2005...

  • Page 41: Program/Process Qc

    Additional References For more information about this procedure, refer to the UniCel DxC Synchron Clinical Systems Instructions For Use manual, CHAPTER 6, Quality Control. UniCel DxC 600/800 In-Lab Training Manual A24183 Procedures and Processes May 2005 Page 3-15...

  • Page 42: Procedure

    Pour the control material into a 0.5 mL or 2.0 mL sample cup. Place the sample cup in the assigned rack/cup position. Place the rack in the autoloader tray. Press the RUN button on the analyzer. Procedures and Processes UniCel DxC 600/800 In-Lab Training Manual A24183 Page 3-16 May 2005...

  • Page 43: Processing Bar Coded Patient Samples

    Load rack in the Autoloader tray. Press the RUN button on the analyzer. UniCel DxC 600/800 In-Lab Training Manual A24183 Procedures and Processes May 2005 Page 3-17...

  • Page 44: Processing Unreadable Bar Code Patient Samples

    • Enter the Rack number. • Enter the SAMPLE IDs. • Select <OK>. Load rack in the Autoloader tray. Press the RUN button on the analyzer. Procedures and Processes UniCel DxC 600/800 In-Lab Training Manual A24183 Page 3-18 May 2005...

  • Page 45: Programming And Processing Patient Samples Manually

    If provided, enter Demographics: • Select Demog [F2]. • Enter in the information provided. • To return to the Program Sample screen, select Next [F10]. (1 of 2) UniCel DxC 600/800 In-Lab Training Manual A24183 Procedures and Processes May 2005 Page 3-19...
  • Page 46 If the sample does not have a readable bar code label: • Refer to "Processing Unreadable Bar Code Patient Samples" in this manual. To program additional samples, repeat Steps 2–8. (2 of 2) Procedures and Processes UniCel DxC 600/800 In-Lab Training Manual A24183 Page 3-20 May 2005...

  • Page 47: Power Down And Power Up

    Press the OFF power switch at the instrument. Press the OFF power switch at the console (i.e., computer). UniCel DxC 600/800 In-Lab Training Manual A24183 Procedures and Processes May 2005...

  • Page 48: Procedure

    To safely restore power to the system follow these steps: Step Action Press the ON power switch at the console (i.e., computer). Press the ON power switch at the instrument. Procedures and Processes UniCel DxC 600/800 In-Lab Training Manual A24183 Page 3-22 May 2005...

  • Page 49: Chapter 4 Training

    Cal Time Left and Days Left. Request and process calibration for all ISE Chemistries, GLUCm and MG. Using the Cal Load List, determine Calibrator IDs and Calibrator Lot #/Level. UniCel DxC 600/800 In-Lab Training Manual A24183 Training May 2005 Page 4-1...
  • Page 50 Program and process 2 levels of Quality Control using bar code labels. Manually program and process 5 samples without bar code labels (included information: routine/stat, patient ID #, sample type, tests and demographics). (1 of 2) Training UniCel DxC 600/800 In-Lab Training Manual A24183 Page 4-2 May 2005...
  • Page 51 Prepare the instrument to "clean flow cell and cups." Perform "CC Probe Cleaning" procedure. Determine Chloride’s Calibration Span and frequency for replacement of chloride’s electrode tip. (1 of 2) UniCel DxC 600/800 In-Lab Training Manual A24183 Training May 2005 Page 4-3...
  • Page 52 What is Beckman Coulter’s Call Center phone number? _____________________________ Describe the laboratory’s procedure for restarting the Host Communications. Status Tech’s Trainer’s Initials / Initials/Date Date View Current Temperature status. View Current Hydropneumatics status. Training UniCel DxC 600/800 In-Lab Training Manual A24183 Page 4-4 May 2005...

  • Page 53: Unicel Dxc Competency Exercise

    9. Replace any reagent that is <10 tests remaining or has < 10% volume remaining. 10. Which MC reagent is replaced monthly? 11. What is the purpose of "No Foam" reagent? UniCel DxC 600/800 In-Lab Training Manual A24183 Training May 2005 Page 4-5...

  • Page 54: Calibration

    Bar Code Label ID? 21. Process 2 levels of control for all general chemistries on the instrument. Samples 22. Describe a scenario wherein the PRIORITY LOAD button is used. Training UniCel DxC 600/800 In-Lab Training Manual A24183 Page 4-6 May 2005...

  • Page 55: Review Results

    28. Recall results for Sample ID # 2222. Print another report for this sample. 29. Request a re-run for GLUCm on Sample ID # 1111. For this exercise, inform the DxC analyzer that you had performed 1:2 dilutions on the sample. UniCel DxC 600/800 In-Lab Training Manual A24183 Training May 2005...

  • Page 56: Udr

    30. Give two differences between a "Beckman Coulter" chemistry and a "UDR" chemistry. Instrument 31. List the steps that you would perform (on the DxC Console) to Home the Commands instrument. Training UniCel DxC 600/800 In-Lab Training Manual A24183 Page 4-8 May 2005...

  • Page 57: Answer Key

    15. IgG, IgA, IgM, and TRF require entering calibration parameters with every new lot of reagent. The parameter card is included with each reagent cartridge. The parameters may be entered manually or with the bar code reader. False True UniCel DxC 600/800 In-Lab Training Manual A24183 Training May 2005 Page 4-9...

  • Page 58: Calibration

    31. List the steps that you would perform (on the DxC Console) to Home the Commands instrument. • At the DxC Console, select the Instrument Commands icon • Select Option 1: Home Training UniCel DxC 600/800 In-Lab Training Manual A24183 Page 4-10 May 2005...
  • Page 59 ® ___________________________________________________________ Has satisfactorily completed the In-Lab Training Course on the operation of the UniCel DxC Clinical System DxC 600 DxC 800 A007386P.EPS _____________________________________ ______________________ Beckman Coulter Representative Date...

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