How The Leadcare Ii System Works - Magellan LeadCare II User Manual

How the LeadCare II System Works

The LeadCare II System uses an electrochemical technique called Anodic
Stripping Voltammetry (ASV) to determine the amount of lead in a blood sample.
The diagram below illustrates the methodology.
1
2
Pb
Pb
Reduction step
3
LeadCare ® II User's Guide
Blood is mixed with LeadCare Treatment Reagent and the red
blood cells (RBC) are lysed, which releases the lead that is
bound to the RBC wall.
Reagent
A negative potential is applied to the sensor to accumulate lead
atoms on the test electrode. The potential is rapidly reversed
releasing the lead ions.
Pb Pb
Pb Pb
Pb
The current produced is directly proportional to the amount of
lead in the sample. The area underneath the curve is used to
calculate a quantitative blood lead result.
Pb +2
Pb +2
Pb +2
Pb +2
Pb
Pb
+2
Oxidation (stripping) step
Analysis
Pb
+2
Pb +2
Pb +2
Pb +2
+2
Pb
+2
1-10