Invitrogen iBright CL1500 Series User Manual
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Invitrogen iBright CL1500 Series User Manual

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™
iBright
Imaging Systems
USER GUIDE
For use with the iBright
CL1500 Imaging System and the iBright
™
™
FL1500 Imaging System
Catalog Numbers A43678, A44114, A44240 (CL1500) and A43679, A44115, A44241 (FL1500)
Publication Number MAN0018592
Revision A.0
For Research Use Only. Not for use in diagnostic procedures.

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  • Page 1 ™ iBright Imaging Systems USER GUIDE For use with the iBright CL1500 Imaging System and the iBright ™ ™ FL1500 Imaging System Catalog Numbers A43678, A44114, A44240 (CL1500) and A43679, A44115, A44241 (FL1500) Publication Number MAN0018592 Revision A.0 For Research Use Only. Not for use in diagnostic procedures.
  • Page 2 Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256 For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition. The information in this guide is subject to change without notice. DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC.

  • Page 3: Table Of Contents

    Contents ■ Product information ............6 Product description .
  • Page 4 Contents ■ Export and analyze images ..........22 Export images .
  • Page 5 Contents Safety and electromagnetic compatibility (EMC) standards ......42 Safety compliance ............42 EMC .

  • Page 6: Product Information

    Product information IMPORTANT! Before using this product, read and understand the information in the “Safety” appendix in this document. Product description ™ The iBright CL1500 Imaging System allows users to image chemiluminescent Western blots, DNA and RNA gels stained with fluorescent nucleic acid stains, and ™...

  • Page 7: Start, Sign On, And Configure The Instrument

    Start, sign on, and configure the instrument Create an instrument profile 1. Touch to sign in to your instrument user account. 2. Touch Get started. 3. Touch Create a Profile. 4. Enter a Screen name. 5. Enter and confirm a four-digit pin. 6.

  • Page 8: Mobile Device

    Start, sign on, and configure the instrument Create an instrument account and link to Connect using a cloud account Mobile device 1. Touch Mobile Device. 2. Launch instrument Connect mobile app. 3. Sign into your Connect account on the mobile app. 4.

  • Page 9: Link To Connect Using An Existing Instrument Account

    Start, sign on, and configure the instrument Link to Connect using an existing instrument account Link to Connect using an existing instrument account Linking to Connect after account creation can only be done during the export process. 1. Click on 2.

  • Page 10: If The Unit Is On And In Active Mode

    Start, sign on, and configure the instrument If the unit is on and in active mode 3. Enter a Screen name. 4. Enter and confirm a 4-digit pin. 5. From the Welcome screen, choose the appropriate Mode from the drop-down menu, touch Open Drawer, and place sample in the center of the transilluminator glass.

  • Page 11: Operate The Ibright ™ Imager

    Operate the iBright imager ™ Choose imaging mode and start image capture On the Welcome screen: 1. Use the drop-down menu to select the desired mode. • Chemi Blots to image chemiluminescent substrates. • Fluorescent Blots to image fluorescent substrates. •...

  • Page 12: Chemi Blots

    Operate the iBright imager ™ Chemi Blots Chemi Blots Mode to image chemiluminescent Western blots. 1. Touch Smart Exposure. Image using Chemi Blots The imager acquires a series of short exposures, then renders a preview image and recommends an exposure time. This is not a real image. 2.

  • Page 13: Fluorescent Blots (Fl1500 Model Only)

    Operate the iBright imager ™ Fluorescent Blots (FL1500 model only) Fluorescent Blots (FL1500 model only) Mode to image fluorescent Western blots using a single channel or up to 4 channels using excitation wavelengths (FWHM) of approximately 455-485 nm, 515-545 nm, 610-635 nm, 610-660 nm, and 750-768 nm;...

  • Page 14: Nucleic Acid Gels

    Operate the iBright imager ™ Nucleic Acid Gels Nucleic Acid Gels Mode to image DNA and RNA gels stained with fluorescent nucleic acid stains such ™ ™ ™ as Ethidium Bromide, SYBR Safe, SYBR Green (I or II), SYBR Gold and similar products.

  • Page 15: Protein Gels

    Operate the iBright imager ™ Protein Gels Protein Gels • Protein Gels Visible: USE WHITE SCREEN BETWEEN SAMPLE AND TRANSILLUMINATOR GLASS for samples including Coomassie-stained protein gels and silver-stained protein gels. • Protein Gels Fluorescent: PLACE SAMPLE DIRECTLY ON ™ TRANSILLUMINATOR GLASS for samples including No-Stain -lableled protein ™...

  • Page 16: Universal Mode

    Operate the iBright imager ™ Universal Mode Universal Mode Mode to image samples containing one or more signal types (chemi, fluor, and/or visible). Table 3 Illumination sources, excitation filters, and emission filters for the iBright ™ CL1500 Instrument. Illumination Excitation Filters Emission Filters White Epi-LED 455-485 nm...
  • Page 17 Operate the iBright imager ™ Universal Mode Channel refers to the type of signal you want to capture and the imaging conditions you want to use. • Chemi: Channel for measuring chemiluminescent signals. • Fluor [Epi-LED]: Channel for measuring fluorescent signals that are excited using the appropriate Epi-LED/excitation filter combination and the appropriate emission filter.

  • Page 18: Options Available In Modes

    Operate the iBright imager ™ Options available in modes Options available in modes Each mode offers More Options on certain workflow screens to provide detailed camera and image adjustment. Table 5 Camera Suboption Detail Changes binning setting to increase resolution/decrease Resolution/Sensitivity sensitivity or decrease resolution/increase sensitivity.
  • Page 19 Operate the iBright imager ™ Options available in modes Suboption Detail Increases or decreases digital zoom to select an area of the Zoom image. Produces a negative or positive (white to black or black to Invert white) of the displayed image. False-colors saturated white pixels (65,536) as red to Saturation differentiate from non-saturated pixels.
  • Page 20 Operate the iBright imager ™ Options available in modes Suboption Detail After a Smart Exposure or Capture, images can also be displayed in a 1UP view. Image displays the grayscale and false-color composite images by toggling Gray or Color. User can switch between Individual channels by touching the dye name you want to display.

  • Page 21: Image Gallery

    Operate the iBright imager ™ Image Gallery Image Gallery The Image Gallery is an on-board storage location where your image files are kept for future viewing. In the gallery you can easily retrieve, analyze and manage your image files. Any image acquired on the imager is automatically saved into a user's individual image gallery.

  • Page 22: Export And Analyze Images

    Export and analyze images Export images Export new From the "Welcome" screen: images 1. Capture an image. See “Choose imaging mode and start image capture“ on page 11. 2. Touch Export. 3. (Optional) Touch Edit to enter a new file name or comments. Touch Save. 4.

  • Page 23: Select An Export File Type

    Export and analyze images Export images Note: The instrument requires a path for connection to a network drive or printer. Consult your IT department for questions regarding your network. The following information may be required to map a network drive: ·...

  • Page 24: Analyze Images

    Export and analyze images Analyze images Analyze images Analyze new 1. Capture an image. See “Choose imaging mode and start image capture“ on page 11. images 2. Touch Analyze. The system automatically finds and displays analysis frames, lanes and bands for the selected image file.

  • Page 25: Analyze Gallery Images

    Export and analyze images Analyze images b. (Optional) Touch Next to display the analysis table. At the top of the table, touch to change the table output by: Output Units Description (Lane and Band Table) Volume Intensity Sum of pixel intensities contained in an identified band.
  • Page 26 Export and analyze images Analyze images 4. Touch Analyze. The system automatically finds and displays analysis frames, lanes and bands for the selected image file. Multi-channel images are displayed as individual channels that can be toggled or swiped between using the touchscreen. The system can identify 1-4 different analysis frames.

  • Page 27: Maintain The Instrument

    Maintain the instrument Settings Access Settings from the "Home" screen. Instrument Settings About Instrument Provides all instrument details including serial number and firmware version. Date and Time Select a Time Zone, Date Format, and Time Format for your region. Sleep Mode Enable or disable a sleep mode by toggling the button.
  • Page 28 Maintain the instrument Settings Event Log Displays instrument error history with sortable columns for Date, User, Event Type, and Cloud Status. Software Update Updates the instrument software package by downloading via USB dongle or connection to the cloud. Note: Cloud option requires a connection to your Connect account. Network Configuration Wired Searches for an ethernet connection.

  • Page 29: Manage Accounts

    Maintain the instrument Run instrument diagnostics Manage accounts Manage accounts allows for editing of user types and PINs and deleting profiles. 1. Touch and log in to your user account. Once you log in, you are automatically taken to the Welcome screen. Note: You must be an Admin logged into your profile to manage accounts.

  • Page 30: Clean The Instrument

    Maintain the instrument Clean the instrument Clean the instrument Repeated instrument use can cause spots and smudges on the sample stage, which can then decrease image quality. Clean the sample stage as needed. Materials required • Safety glasses • Powder-free gloves •...

  • Page 31: Move The Instrument

    Maintain the instrument Move the instrument 3. Remove each fuse from its fuse holder and inspect it for damage. Carbon typically coats the inside of failed fuses. 4. Replace each failed fuse with a 1.6A, Time-Lag T, 250VAC, 5 × 20-mm Fuse. Note: The voltage and amperage ratings are on the fuse holder.
  • Page 32 Maintain the instrument Move the instrument 3. Touch Ship prep mode. Drawer opens. 4. Remove turntable. 5. Touch Next. 6. Power off the instrument. iBright Imaging Systems User Guide ™...

  • Page 33: Instrument Specifications

    Instrument specifications Instrument dimensions and specifications Specification iBright ™ Imager Dimensions (L × W × H) 68.0 × 38.2 × 59.8 cm Weight 52 kg (115 lbs) Instrument clearance (Back) 15.25 cm (6 in.) Electrical requirements WARNING! For safety, the power outlet used for powering the instrument must be accessible at all times.

  • Page 34: Network Requirements

    Instrument specifications Network requirements Condition Acceptable range • Humidity: 15–80% relative humidity (noncondensing) Operating conditions • Temperature: 15 to 30°C (59 to 86°F) Note: For optimal performance, avoid rapid or extreme fluctuations in room temperature. • Humidity: 20–80% relative humidity (noncondensing) Storage and transport conditions •...

  • Page 35: Instrument And Computer Connections

    Instrument specifications Instrument and computer connections Required materials to network the instrument: • Wired—Ethernet cable of sufficient length with RJ45 connectors – CAT5 cable for a 100 Mbps network connection –or– • Wireless—High Power USB WiFi Module (Cat. No. A26774, sold separately) Instrument and computer connections iBright Imager front view...
  • Page 36 Instrument specifications Instrument and computer connections iBright ™ Imager rear view USB port Ethernet port — RJ45 port for 10/100 Mbps ethernet communication with the instrument Fuse cover Power switch Power port — 100-240 VAC iBright Imaging Systems User Guide ™...

  • Page 37: Safety

    Safety WARNING! GENERAL SAFETY. Using this product in a manner not specified in the user documentation may result in personal injury or damage to the instrument or device. Ensure that anyone using this product has received instructions in general safety practices for laboratories and the safety information provided in this document.

  • Page 38: Safety Alerts On This Instrument

    Safety Symbols on this instrument Symbol English Français Protective conductor terminal (main Borne de conducteur de protection ground) (mise à la terre principale) Do not dispose of this product in Ne pas éliminer ce produit avec les unsorted municipal waste déchets usuels non soumis au tri sélectif.

  • Page 39: Location Of Safety Labels On The Instrument

    Safety Safety information for instruments not manufactured by Thermo Fisher Scientific Location of safety labels on the instrument Safety information for instruments not manufactured by Thermo Fisher Scientific Some of the accessories provided as part of the instrument system are not designed or built by Thermo Fisher Scientific.

  • Page 40: Led (Light-Emitting Diode)

    Safety Instrument safety LED (Light- CAUTION! LED (light-emitting diode) HAZARD. Removing the protective Emitting Diode) covers and (when applicable) defeating the interlock(s) may result in exposure to the internal LED. LEDs can burn the retina, causing permanent blind spots. To ensure safe LED operation: ·...

  • Page 41: Instrument Component And Accessory Disposal

    Safety Instrument safety Instrument To minimize negative environmental impact from disposal of electronic waste, do not dispose of electronic waste in unsorted municipal waste. Follow local municipal waste component and ordinances for proper disposal provision and contact customer service for information accessory about responsible disposal options.

  • Page 42: Safety And Electromagnetic Compatibility (Emc) Standards

    Safety Safety and electromagnetic compatibility (EMC) standards Safety and electromagnetic compatibility (EMC) standards The instrument design and manufacture complies with the following standards and requirements for safety and electromagnetic compatibility. Safety compliance Reference Description EU Directive European Union “Low Voltage Directive” 2014/35/EU Safety requirements for electrical equipment for measurement, IEC 61010-1...

  • Page 43: Environmental Design

    Safety Safety and electromagnetic compatibility (EMC) standards Environmental Reference Description design EU Directive European Union “WEEE Directive”—Waste electrical and electronic 2012/19/EU equipment EU Directive European Union “RoHS Directive”—Restriction of hazardous 2011/65/EU substances in electrical and electronic equipment EU Directive European Union “Battery Directive” 2006/66/EC GB/T 26572-2011 Requirements of concentration limits for certain restricted...

  • Page 44: Chemical Safety

    Safety Chemical safety Chemical safety WARNING! GENERAL CHEMICAL HANDLING. To minimize hazards, ensure laboratory personnel read and practice the general safety guidelines for chemical usage, storage, and waste provided below. Consult the relevant SDS for specific precautions and instructions: · Read and understand the Safety Data Sheets (SDSs) provided by the chemical manufacturer before you store, handle, or work with any chemicals or hazardous materials.

  • Page 45: Biological Hazard Safety

    Safety Biological hazard safety Biological hazard safety WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. Conduct all work in properly equipped facilities with the appropriate safety equipment (for example, physical containment devices).

  • Page 46: Documentation And Support

    Documentation and support Related documentation Document Publication number Description iBright Imaging Systems ™ 100085109 Provides instructions for Documentation obtaining service and technical support for the ™ iBright Imaging Systems. iBright Imaging Systems ™ 100085108 Provides basic instructions Quick Reference for performing imaging with the iBright ™...
  • Page 48 thermofisher.com/support | thermofisher.com/askaquestion thermofisher.com 13 June 2019...

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