Figure 8 "Checking Stroke Volume - UGO BASILE 7025 Series Instruction Manual

Attach a clean, freely moving 10ml (or 30ml) glass syringe via a short (5cm)
B.
length of tubing to the stem of a "Y" connector. Adjust the plunger so that 1-2
ml (or 3-6 ml) of air is in the syringe, before attaching the tube.
Attach the two arms of a "Y" to the "room air" and "to animal" ports of the valve
C.
block. Use short lengths of tubing. This will establish a closed system between
the pump cylinder and the syringe, so that the air will move back and forth from
pump to syringe.
Set the speed at about 30 S.P.M. and observe the plunger excursion as it
D.
moves back and forth. Hold the syringe horizontally to minimize gravity effect
on the plunger travel. The limits of its travel will indicate the actual amount of
air being supplied by the pump at each stroke.
To check stroke to stroke volume, use a freely moving 50 ml glass syringe and
connect it with a short length of Tygon® or rubber tubing to the "to animal" port.
With the piston in the max. stroke position, run the pump at about 40 S.P.M.
and observe how many strokes it takes to get 50 ml. On the 5ml setting, for in-
stance, it should take 10 strokes.
Should the foregoing test show an error in the volume output of the pump, the most
probable cause is air leakage, see paragraph 7.1-Air Leakage.
6.2 Frequency
To check the frequency (strokes per minute), use a stopwatch. Run the pump at 60
S.P.M., i.e., one stroke per second. Count 10 piston strokes then stop the watch.
7025 Instruction Manual (Rev. 2)

Figure 8 "Checking Stroke Volume"

Figure 9 "Checking Stroke to Stroke Volume"
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