Biocontrol Assurance GDS Rotor-Gene Q User Manual

Genetic detection system

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User Manual
55190.R000.082010

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Summary of Contents for Biocontrol Assurance GDS Rotor-Gene Q

  • Page 1 User Manual 55190.R000.082010...
  • Page 2: Table Of Contents

    Assurance GDS User Manual ® Assurance GDS _____________________________________________________________ 3 Overview________________________________________________________________________ 3 Required Materials _______________________________________________________________ 3 Designated Assurance GDS Equipment ____________________________________________ 3 Assurance GDS Rotor-Gene ___________________________________________________ 4 Components_____________________________________________________________________ 4 Operating Conditions ____________________________________________________________ 4 Dimensions ______________________________________________________________________ 4 Installation ______________________________________________________________________ 4 Rotor-Gene (front view) __________________________________________________________ 5 Rotor-Gene (rear view) ___________________________________________________________ 5 Loading the Assurance GDS Rotor-Gene ___________________________________________ 6 Unloading the Assurance GDS Rotor-Gene _________________________________________ 6...
  • Page 3: Assurance Gds

    Assurance GDS Test Kit Polymerase Enzyme Instrumentation and Supplies Additional instrumentation and supplies are required to perform Assurance GDS assays. All instrumentation is included in the Assurance GDS start-up package. Supplies are available for purchase through BioControl Systems, Inc. Instrumentation Supplies...
  • Page 4: Assurance Gds Rotor-Gene

    The Assurance GDS Rotor-Gene can be connected to the communications port of the Assurance GDS lap top computer (provided by BioControl) via either the serial cable or USB cable included with the Assurance GDS Rotor-Gene. It is important to only use the authorized laptop provided by BioControl with the Assurance GDS Rotor-Gene.
  • Page 5: Rotor-Gene (Front View)

    Assurance GDS User Manual Rotor-Gene (front view) Air vents Lid handle Instrument status lights Rotor chamber Exhaust vent Rotor-Gene (rear view) Power switch Power supply port USB port Serial number Cooling fan Serial port 55190.R000.082010...
  • Page 6: Loading The Assurance Gds Rotor-Gene

    Assurance GDS User Manual Loading the Assurance GDS Rotor-Gene Turn on the Assurance GDS Rotor-Gene. Open the Assurance GDS Rotor-Gene by pushing the cover Locking ring Rotor back. Ensure that the rotor is correctly positioned in the Rotor- Gene by aligning the hole in the center circle of the rotor with the alignment pin on the Rotor-Gene.
  • Page 7: Assurance Gds Rotor-Gene Software

    Operators have the ability to generate a report that can be saved to a file, printed, or e- mailed directly from the GDS Rotor-Gene software. Necessary passwords can be obtained from BioControl Technical Services. 55190.R000.082010...
  • Page 8: Entering Sample Information

    Assurance GDS User Manual Entering Sample Information The Rotor-Gene software opens to the Setup menu shown below. A new run is automatically opened each time the Rotor-Gene software is launched. Operator: Enter name of user. Notes: Enter comments or other description of run. Name: Click in the desired cell to enter sample names.
  • Page 9: Viewing Results

    Assurance GDS User Manual Note: to select the same assay type, lot number, or description for multiple samples: Click the first cell within the corresponding column. Drag the pointer or use shift+arrow key to highlight the desired cells Type in the description or lot number for this series or for assay type, select the desired assay type from the drop-down menu.
  • Page 10 Assurance GDS User Manual Viewing Results as GDS Operator When logged in as a “GDS Operator” the Assurance GDS Rotor-Gene software will display results in the table format only. Viewing Results as GDS Analyst When logged in as a “GDS Analyst” the Assurance GDS Rotor-Gene software will display amplification curves for the “Test Samples”...
  • Page 11: Interpreting Results

    Negative: Samples test negative for target organism and internal control is positive Amp: Sample tests negative for target organism and internal control is negative. This means that amplification did not occur. User should go back to enriched sample and re-run assay. If problem persists, contact BioControl Technical Services. 55190.R000.082010...
  • Page 12: Virtual Mode / Analysis Only Mode

    Selecting the Reports button opens the report screen shown below. BioControl Analysis (Concise) – This report generates a results table for each assay. BioControl Analysis (Full Report) – This report generates a results table and amplification curves for each assay – Analyst level users only.
  • Page 13: Maintenance

    Keep the bench area clean and free from dust and sheets of paper. The air inlet of the Assurance GDS Rotor- Gene is at the bottom and loose material such as paper or dust may compromise performance. Service and Support For technical assistance or questions about the Assurance GDS Rotor-Gene or system please contact: BioControl Technical support at 1.800.245.0113 55190.R000.082010...
  • Page 14: Contamination Prevention

    Assurance GDS User Manual Contamination Prevention Assurance GDS™ is a highly sensitive and specific test based on the principles of DNA amplification and detection. Due to the sensitive nature of this assay certain precautions must be taken to avoid potential sample contamination and produce accurate test results.
  • Page 15 Assurance GDS User Manual Work Zone 3 – Amplification & Detection This area should contain a suitable work surface and power source for the Assurance GDS Rotor-Gene and lap top workstation. No cultures, food samples or disposable supplies should be stored or prepared in this area.
  • Page 16: Recommended Laboratory Practices For Contamination Control

    Assurance GDS User Manual Recommended Laboratory Practices for Contamination Control The equipment supplied with the Assurance GDS start-up package must be designated for use with Assurance GDS assays only. Use of any of these instruments including pipettors for other general microbiology work may lead to contamination of samples and/or reagent solutions.
  • Page 17: Routine Cleaning And Decontamination Of Assurance Gds

    Assurance GDS User Manual ® Routine Cleaning and Decontamination of Assurance GDS Equipment Wipe down laboratory surfaces and pipettes with a 10-20% bleach solution before and after use. To avoid corrosion of metal surfaces, wipe away bleach solution and wipe down surface with a 70% alcohol solution. At least once per week, perform preventive cleaning with bleach: Remove the rotor and locking ring and soak in 20% bleach solution for 10 minutes (no longer).
  • Page 18: Assurance Gds Equipment Warranty Terms

    (“Buyer”). BioControl agrees during the applicable warranty period to repair or replace, at BioControl’s option, all defective equipment within 5 days after date of return to BioControl and without cost to Buyer.
  • Page 19 Assurance GDS User Manual 55190.R000.082010...

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