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Biocontrol CL-10 Plus User Manual

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Summary of Contents for Biocontrol CL-10 Plus

  • Page 1 CL-10 Plus User Manual...

  • Page 2: Table Of Contents

    Connection cables. _______________________________________________________________6 2.3.4 Software Installation and configuration______________________________________________7 2.3.5 Hydraulic connections. ____________________________________________________________9 2.3.6 Internal & peristaltic pump tubing’s _______________________________________________10 2.4. CL-10 Plus switching on. _____________________________________________________________11 2.5. Instrument Stabilisation._____________________________________________________________11 2.5.1 Materials.______________________________________________________________________11 2.5.2 Peristaltic starter tube sealing control._____________________________________________11 2.5.3 Electrodes regeneration. _________________________________________________________12 2.5.4...
  • Page 3 Unstable Measurements . _________________________________________________________66 Electrodes. ________________________________________________________________________66 6.3.1 Control of electrodes performances ________________________________________________66 6.3.2 Electrodes replacement. _________________________________________________________68 6.4. CL-10 Plus troubleshooting – rapid guide________________________________________________71 Materials. _________________________________________________________________________73 Using the Microman Pipette.__________________________________________________________73 7.2.1 How to Mount the Piston. ________________________________________________________73 7.2.2 How to mount the capillary. ______________________________________________________73 Pipetting.__________________________________________________________________________74...
  • Page 4 CL-10 Plus 1. Introduction CL-10 Plus instrument is an automatic multi-parametric analyser, working in differential-pH technique for chemical analyses in various type of matrices, from food industry to clinical applications. Technique characteristics are: - Operative simplicity on different matrices, also particularly difficult ones.

  • Page 5: Unpacking And Checking

    CL-10 Plus is delivered with the fluidics empty (free from liquid) and dry and with the peristaltic pumps unhooked (see § 5.5 figure; the detail of the pump tubing’s).

  • Page 6: System Installation

    The power fluctuation must be secured < 5%. If not, it is recommended to connect the CL-10 Plus instrument and the PC to a 500VA stabilizer. In any case, it is recommended to keep the CL10 Plus instrument under UPS (Uninterruptible Power Supply), for protection against accidental power loss.

  • Page 7: Software Installation And Configuration

    Point the mouse anywhere on the desktop Press the mouse right-hand button and select: new link. Once the new window appears, search for the following path: C:\cl10\ cl10plus Select Next, and then End. CL-10 Plus – User Manual 7 / 75 ECPUS vers 0.2 eng...
  • Page 8 15. Change properties of the pasted files; from read only into archive. 16. Unable the screen saver and the energy save setting, in order to avoid any interference during the working session. CL-10 Plus – User Manual 8 / 75 ECPUS vers 0.2 eng...

  • Page 9: Hydraulic Connections

    Careful that the ’L’ connections are not pushed to the far end of the silicon but only halfway. 3. Place the bottle in the waste position. ENZIMI/STARTER SCARICO/WASTE TAMPONE/BUFFER Fig. 2 CL-10 Plus – User Manual 9 / 75 ECPUS vers 0.2 eng...

  • Page 10: Internal & Peristaltic Pump Tubing's

    WCP678, only (pump tubing’s 5-6, Fig 2 § 2.3.5) SPP 315: product for WCP682, only (pump tubing’s 5-6, Fig 2 § 2.3.5) SPP 316: product for BCP 592-596-690, only (pump tubing’s 5-6, Fig 2 § 2.3.5) CL-10 Plus – User Manual 10 / 75 ECPUS vers 0.2 eng...

  • Page 11: Cl-10 Plus Switching On

    It is very important to connect it gently, taking care to the final position of the yellow ring embracing firmly the silicon of the starter tubing and the steel capillary on top of the mixing chamber. CL-10 Plus – User Manual 11 / 75...

  • Page 12: Electrodes Regeneration

    Clean cycles. Empty the waste bottle. 2.5.4 Standby mode (Rest) For the CL-10 Plus instrument there are two available different standby mode (REST mode): From the computer, REST mode program ( § 2.3.4). From Service window enter Enter Rest Mode command...

  • Page 13: Instrument Stabilisation With Wash Solution

    Check that the AUX tubing, the longer (connected to pump 5), is plugged to the top of the mixing chamber, too. Follow the instructions on the Package Insert of EC Wine COMBI test. CL-10 Plus – User Manual 13 / 75 ECPUS vers 0.2 eng...

  • Page 14: Starting And Calibrating

    Replace starter vial (and AUX if used) with a vial containing distilled water (min 3 mL). Run 2 Prime enzyme (F2) and 2 Clean cycles (F3). Empty the waste bottle. Leave the instrument in standby condition or Rest mode. CL-10 Plus – User Manual 14 / 75 ECPUS vers 0.2 eng...

  • Page 15: Changing Of Application Within The Same Working Session

    (starter) of the next chosen method. From File menu, Select the next method. Empty the waste bottle. Follow the instructions given in the package insert of the method to use. CL-10 Plus – User Manual 15 / 75 ECPUS vers 0.2 eng...

  • Page 16: The Principle

    (AUX) n°6: starter pump n°7: electrode 1 n°8: electrode 2 n°9: mixing chamber ENZIMI/STARTER SCARICO/WASTE TAMPONE/BUFFER Figure 2 § 2.3.5: CL-10 Plus – User Manual 16 / 75 ECPUS vers 0.2 eng...

  • Page 17: Titration Curve And Buffer Power Measurement

    The titration curve is a reversible reaction (adding H back to the system, acetate is converted back to the initial state). CL-10 Plus – User Manual 17 / 75 ECPUS vers 0.2 eng...
  • Page 18 BUFFER POWER measurement, using CL-10 Plus instrument. The working buffer, using CL-10 Plus, must provide a known and constant Buffer Power. Sensitivity of the method depends on sample volume, stoichiometry and Buffer Power. Sample:...
  • Page 19 2. The buffer solution is maintained in the electrode 1. The pH change due to HCL acidification is instantaneous. 15 seconds are allowed to electrodes stabilisation, then the result is displayed on the screen. CL-10 Plus – User Manual 19 / 75 ECPUS vers 0.2 eng...

  • Page 20: End Point Measurement

    PHASE ZERO: the mixing chamber and the electrodes are filled with buffer, ready to accept the sample. PHASE 1: using the micropipette, inject 25 µL of milk in the mixing chamber; the stirrer rotates and mixes buffer + milk for some seconds. CL-10 Plus – User Manual 20 / 75 ECPUS vers 0.2 eng...
  • Page 21 (in fact between ± 150 mpH). The signal measured will be subtracted during the following measure. Example: during the measurement the obtained ∆mpH is 20, the following graph ∆mpH/time will be displayed ∆mpH t (s) CL-10 Plus – User Manual 21 / 75 ECPUS vers 0.2 eng...
  • Page 22 (thus the difference EL 2 - EL 1) until all urea in the sample has been fully transformed. In the graph we following the reaction is displayed: CL-10 Plus – User Manual 22 / 75 ECPUS vers 0.2 eng...
  • Page 23 For these reasons, during routine, where not all the approximations made are necessarily true, the operator will run a calibration procedure, before any sample. Hereafter there is an explanation of the meaning of the calibration procedure, in detail: CL-10 Plus – User Manual 23 / 75 ECPUS vers 0.2 eng...
  • Page 24 So, if you remember the graph of our milk sample, not all the 10 ∆mpH are caused by the urea hydrolysis, 1 ∆mpH is in fact due to the enzyme contribution: ∆mpH (DL1) t (s) D1 time D2 time CL-10 Plus – User Manual 24 / 75 ECPUS vers 0.2 eng...
  • Page 25 The instrument, after calibration, will store in the method settings the OFFSET and SLOPE values and calculate the concentration (of the samples unknown) according to the formula [sample] = [∆mpH sample - offset] * slope CL-10 Plus – User Manual 25 / 75 ECPUS vers 0.2 eng...

  • Page 26: Kinetic Measurement (Kin)

    Pumps 1 and 2 rotate and both the electrodes are filled with buffer + blood. The electrodes contain exactly the same solution, the ∆pH should be zero. CL-10 Plus – User Manual 26 / 75 ECPUS vers 0.2 eng...
  • Page 27 5 sec. of D1 phase, period in which the reaction is not yet stable ), starting at second 45 until the end of measure phase (second 75). CL-10 Plus – User Manual 27 / 75 ECPUS vers 0.2 eng...
  • Page 28 (in method settings), to obtain the result in U/L. PHASE 4: the instrument is automatically washed with buffer and the mixer refilled with the new buffer. CL-10 Plus – User Manual 28 / 75 ECPUS vers 0.2 eng...

  • Page 29: Fixed Time Measurement

    + sample for some seconds and both electrodes are filled with buffer + sample. The electrodes contain the same solution; the ∆pH should be zero. CL-10 Plus – User Manual 29 / 75 ECPUS vers 0.2 eng...
  • Page 30 The result calculation (slope of ∆pH curve) is performed skipping D1 (5 sec.) and the Lag Phase (5 sec.), beginning at second 10 until the end of measure phase is reached. CL-10 Plus – User Manual 30 / 75 ECPUS vers 0.2 eng...
  • Page 31 Lag Phase with ∆mpH is lower than ∆mpH. PHASE 4: the instrument is automatically washed and the mixing chamber refilled with the new buffer. CL-10 Plus – User Manual 31 / 75 ECPUS vers 0.2 eng...

  • Page 32: Menu And Commands

    4. The CL-10 Plus Program 4.1. Menu and commands. The following list describes the sections of the figure above: A) Main menu and submenu If in the Menu or submenu the chose command name starts with an underlined letter, it is possible to activate the command pressing: ALT + the underlined letter, in order to enter the menu (ex.
  • Page 33 E) F) Parameter name It is a "non editable section" from this window (see method editing option at § 4.2.3); the name of the method described in the picture is COMBITEST giving three parameters ( pH, total acidity, glucose + fructose). CL-10 Plus – User Manual 33 / 75 ECPUS vers 0.2 eng...

  • Page 34: File Menu

    Attention: select a method only after choosing the kit intended to be used for the routine; in the package insert of the kit the name of the method (*.MD file) corresponding to the program is specified . CL-10 Plus – User Manual 34 / 75 ECPUS vers 0.2 eng...

  • Page 35: Create A New Method

    If the version number of the method (*.MD) does not correspond to the same in the package insert, contact costumer service. ∗ Modify the method parameters, as described, then select SAVE to confirm the changes. Figure 1 CL-10 Plus – User Manual 35 / 75 ECPUS vers 0.2 eng...
  • Page 36 Time (sec.): total measure time from the selection of GO command (or F5 function key) to the end of the measure and the start of cleaning (see § 3.2.3) CL-10 Plus – User Manual 36 / 75 ECPUS vers 0.2 eng...
  • Page 37 The slope is then used by the program itself during samples analysis, according to the following equation, to relate sample signal to its concentration: Sample concentration = Slope x Sample signal CL-10 Plus – User Manual 37 / 75 ECPUS vers 0.2 eng...
  • Page 38 This function can be used to increase the options of a method; in the next § 4.2.4 a very important example is made for using this function in Biochemical Chemistry applications CL-10 Plus – User Manual 38 / 75 ECPUS vers 0.2 eng...

  • Page 39: Add Method For Measure Unit Change; Biochemical Chemistry Application

    The default measure unit for erythrocyte enzymes concentration (G6PD, PK, ACHE, BUCHE) used by CL-10 Plus is U/L. However, it is possible to express the erythrocyte enzymes concentration also in U/g Hb, directly from CL-10 Plus Software, modifying the method (file MD).
  • Page 40 This value must be manually introduced by the operator at this stage for each sample, in order CL-10 Plus – User Manual 40 / 75...

  • Page 41: Clear Graph

    4.2.7 Method name It is possible to make a quick select method in the list of the last five methods used, accessing from File menu . CL-10 Plus – User Manual 41 / 75 ECPUS vers 0.2 eng...

  • Page 42: Start Up Menu

    The icon for the command is ; the function key is F2 CL-10 Plus – User Manual 42 / 75 ECPUS vers 0.2 eng...

  • Page 43: Clean Or F3

    If, at the end of the start up procedure, the conditions described in the Start up parameters are not fulfilled, an error message will appear, asking to repeat the startup procedure (see picture underneath). CL-10 Plus – User Manual 43 / 75 ECPUS vers 0.2 eng...
  • Page 44 ∗ First case (Maximum error =0.5): ∗ Second case (Maximum error =0.5): The startup procedure failed The startup procedure passed CL-10 Plus – User Manual 44 / 75 ECPUS vers 0.2 eng...

  • Page 45: Routine Menu

    Sample ID and Sample Name fields identify the sample, then select Start Measure to start the measurement and Accept to confirm the inserted data. CL-10 Plus – User Manual 45 / 75 ECPUS vers 0.2 eng...

  • Page 46: Blank (B Icon Or F6)

    This function allows to stop immediately the measurement, without waiting to the end of the time set. The icon for this command is This function is enabled only after the D1 reading. CL-10 Plus – User Manual 46 / 75 ECPUS vers 0.2 eng...

  • Page 47: Woklist Menu

    The paper layout of the printed results can be customised, in the following way: 1. Exit the CL-10 Plus program 2. Opne the CL-10 plus (C:\cl10 plus) folder 3. Open the ‘’rptstruct.ini’ file (open as text file .txt) rptstruct.ini 4.

  • Page 48: Edit Worklist

    This function is used to export data into text (.txt) or excel (.xls) format. This format (excel) enables the visualisation, editing and data transfer into other databases. To export into excel format, follow the procedure: 1. Select Export Worklist command: CL-10 Plus – User Manual 48 / 75 ECPUS vers 0.2 eng...
  • Page 49 10. Open the excel program from Start menu and open the saved file; The Auto- composition text Import will appear 11. Select the option limited and continue clicking on Next > CL-10 Plus – User Manual 49 / 75 ECPUS vers 0.2 eng...
  • Page 50 13. During the third and last step you can decide which column to transfer or delete, which format to give. After these steps you can select the Finish command to close the data formatting CL-10 Plus – User Manual 50 / 75 ECPUS vers 0.2 eng...

  • Page 51: Service Menu

    (§ 4.6.3). Enter rest mode command remains blinded in case it is not activate from Instrument parameters (§ 4.6.3). 4.6.3 Instrument parameters (Access password: default). This command defines working and Rest mode instrument configuration. CL-10 Plus – User Manual 51 / 75 ECPUS vers 0.2 eng...
  • Page 52 ∗ time interval between consecutive cycles: Period (min); normally set at 90 min ∗ the cycle type used in the rest mode is the same displayed on the program. CL-10 Plus – User Manual 52 / 75 ECPUS vers 0.2 eng...

  • Page 53: Calibrate Temperature

    4.6.4 Calibrate Temperature. This command is only used by technical personnel. CL-10 Plus contains in the hardware all information for the right temperature during the working session. To check that this setting is not idle: From Explorer Enter CL10 folder and open the text file (.txt): C:\cl10\cl10.ini: cl10.ini...

  • Page 54: Terminal Emulation

    Digit ‘+!‘ in sequence: all the numbers and respective commands appear. Digit ‘5’ (ref: tst Hall sensors); on the screen appear numbers in sequence, as descbed in figure 3. Figure 2 CL-10 Plus – User Manual 54 / 75 ECPUS vers 0.2 eng...
  • Page 55 Terminal Emulation and CL-10 working program, before accessing another Terminal Emulation command. Switch CL-10 Plus off and on again, then access the working program and enter Terminal Emulation mode; then procede as follows: Digit ’*’ (asterisk) and wait for the screen to be filled with symbols, as in figure 1 Digit ‘+!‘...

  • Page 56: Electrodes Test 1 And 2

    For proceeding to the test select TEST-EL R3.MD method from Select method and operate following instructions from an representative and from package insert instructions: Art. No. GEN 603. CL-10 Plus – User Manual 56 / 75 ECPUS vers 0.2 eng...

  • Page 57: Weekly Maintenance

    7. Run 1 Prime enzyme and 2 Clean. 8. Replace the distilled water with reconstituted wash solution. 9. Run 2 Clean cycles. 10. Leave the instrument in Rest mode (see § 4.6.2). CL-10 Plus – User Manual 57 / 75 ECPUS vers 0.2 eng...

  • Page 58: Maintenance – Rapid Guide

    If the line is flat but does not overlap to the see § 6.2.3 next cycle one, there is possibly a cloth in the fluidics or enzyme contamination in the reference electrode CL-10 Plus – User Manual 58 / 75 ECPUS vers 0.2 eng...

  • Page 59: Enzyme Contamination In The Reference Electrode

    The enzyme could be damaged or 2) a blockage in the system. expired; replace with a new vial from the same kit or another kit of the same Lot n°. CL-10 Plus – User Manual 59 / 75 ECPUS vers 0.2 eng...
  • Page 60 Figure 1 Figure 2. CL-10 Plus – User Manual 60 / 75 ECPUS vers 0.2 eng...

  • Page 61: Cl10 Preparation For A Period Of Halt (Over 2 Weeks)

    5. Unhook all 6 peristaltic pumps tubing’s from their seats around the peristaltic pumps and leave them as described in picture underneath. 6. Exit the CL-10 Plus program (select Exit from File menu). 7. Switch the instrument and the PC off.
  • Page 62 3. Switch CL-10 Plus on from the instrument rear panel. 4. Wait for about 2 minutes to the end of the first standby cycle. 5. Enter the CL-10 Plus working program. 6. Run three Clean cycles using the clean icon or F3 function key.

  • Page 63: Electrical Section

    (Fig. 1) (if traces of salts and dirt are found, remove using a moistened cloth). FIGURE 1: CL10 PERISTALTIC PUMP SET CHASSY LEFT PERISTALTIC PUMPS GROUP RIGHT PERISTALTIC PUMPS GROUP ALLEN STEEL SCREW MOTOR FLAT CABLE CL-10 Plus – User Manual 63 / 75 ECPUS vers 0.2 eng...

  • Page 64: The Analytical Unit Does Not Accept Commands From The Program

    COM 1, or select the COM 2 port from Communication Parameters in SERVICE menu (see following windows): • If the problem persists, ask for technical assistance. CL-10 Plus – User Manual 64 / 75 ECPUS vers 0.2 eng...

  • Page 65: The Mixer Does Not Fill-In Or Empty

    7. Repeat the same procedure with electrode 2 (pump 2). 8. Run 4 Clean cycles using clean icon or F3 function key and leave the instrument in standby until the following working session. CL-10 Plus – User Manual 65 / 75 ECPUS vers 0.2 eng...

  • Page 66: Absence Of Signal During The Measurement

    Gilson Microman M25 Injection volume: 24 µL Report: Electrodes performance CL-10 Plus Report Test Electrodes Procedure From File menu, trough Select method select: TEST-EL R3.MD Attention: Check the version number (R3= release 3) to be the same in the program as in the package insert of the kit, if not, call technical assistance before proceeding.
  • Page 67 (the same where also the other end C, is placed) 3. Follow the instructions of package insert and fill in the form: Report Electrodes performance CL-10 Plus (available upon request and included in the kit GEN603). FIGURE 2: STARTER line 4.

  • Page 68: Electrodes Replacement

    Push gently the electrodes holder (Fig. 3, part G) towards the electrodes; fix it using the screw. CL-10 Plus – User Manual 68 / 75 ECPUS vers 0.2 eng...
  • Page 69 MEASURING CHAMBER SILICON TIPS FLUIDIC’S GLASS ELECTRODES ELECTRODES HOLDER SCREW ELECTRODE CABLE ELECTRODES HOLDER FIGURE 3: Electrodes Installation STEEL COVER BLACK STOPPER O’RING FRONT PANEL PLEXIGLASS CL-10 Plus – User Manual 69 / 75 ECPUS vers 0.2 eng...
  • Page 70 FIGURE 4: MIXING CHAMBER CHASSIS LEFT PERUISTALTIC PUMP GROUP RIGHT PERISTALTIC PUMP GROUP EXHAGONAL SCREW FLAT CABLE FIGURE 5: PERISTALTIC PUMP GROUP CL-10 Plus – User Manual 70 / 75 ECPUS vers 0.2 eng...

  • Page 71: Cl-10 Plus Troubleshooting – Rapid Guide

    6.4. CL-10 Plus troubleshooting – rapid guide SYMPTOM CAUSE SOLUTION The switch on the back is in OFF Turn the switch on the back to the position position ON The green lamp on the front does not The power supply is disconnected...
  • Page 72 Damaged CPU mother board Replace CPU or ask technical assistance for repair . CL-10 Plus – User Manual 72 / 75 ECPUS vers 0.2 eng...

  • Page 73: Materials

    Slide the mounted piston into the capillary (colour matching the push button). Gently push the capillary back until it snaps onto the pipette shaft. IF THE CAPILLARY TENDS TO SLIP OFF THE SHAFT, CLEAN THE SHAFT WITH TISSUE PAPER AND ALCOHOL. CL-10 Plus – User Manual 73 / 75 ECPUS vers 0.2 eng...

  • Page 74: Pipetting

    RELATIVE ABSOLUTE RELATIVE µL µL S.D. C.V. µL ±0.15 ±5.0 ≤0.06 ≤2.0 ±0.15 ±3.0 ≤0.06 ≤1.2 ±0.17 ±1.7 ≤0.08 ≤0.8 ±0.20 ±1.0 ≤0.10 ≤0.5 ±0.25 ±1.0 ≤0.10 ≤0.4 CL-10 Plus – User Manual 74 / 75 ECPUS vers 0.2 eng...

  • Page 75: How To Inject The Sample

    - REPLACE THE CAPILLARY AND PISTON IF YOU NOTICE SOME LIQUID INTO THE CAPILLARY, BEYOND THE PISTON TIP. - AFTER EVERY INJECTION WIPE THE CAPILLARY, OTHERWISE YOU MAY CONTAMINATE THE NEXT SAMPLE. CL-10 Plus – User Manual 75 / 75 ECPUS vers 0.2 eng...

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