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Related Manuals for Raptor Eagle EA4240-CL
Summary of Contents for Raptor Eagle EA4240-CL
- Page 1 Eagle Models: EA4240-CL and EA4710-CL USER MANUAL...
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Page 2: Table Of Contents
CONTENT CONTENT ..............................2 INTRODUCTION ........................... 3 SPECIFICATION ..........................4 GETTING STARTED ........................6 EPIX XCAP ............................ 9 MICRO-MANAGER ........................19 EA4240-CL and EA4710-CL / USER MANUAL Rev1.3 February 2018... -
Page 3: Introduction
INTRODUCTION Scope This manual covers the Eagle V digital camera and all applicable components. Raptor recommends that this manual be used to optimize camera operation. Camera Care Raptor cameras require no regular maintenance except occasional external cleaning of the sensor window (the glass window between the camera sensor and the microscope or lens). -
Page 4: Specification
SPECIFICATION Camera Specification The EAGLE V digital camera is designed for high-resolution applications requiring visible imaging (300- 1050nm). The EAGLE V camera uses a choice of back-illuminated CCD sensors with a resolution of 1024x1024 or 2048x2048, both with 16-bit digital outputs. Low-noise electronics provide linear response and sensitivity. - Page 5 Mechanical Outline 3D drawings (STEP) are available upon request. EA4240-CL and EA4710-CL / USER MANUAL Rev1.3 February 2018...
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Page 6: Getting Started
GETTING STARTED System Overview F-mount Water taps Camera Link Connector/MDR Trig out/SMA - Trig in/SMA Exposure Trig out/SMA – Power Readout Lenses The EAGLE V has a standard Nikon F-Mount for standard lenses. It features a three-lug bayonet mount with a 44 mm throat and a flange to focal plane distance of 46.5 mm. Mounting to a tripod or optical table The camera has three (x3) ¼-20 BSW (Whitworth), threaded holes to mount to a tripod(s) or an optical table. - Page 7 PARTY SOFTWARE Raptor supports a range of 3 party software packages as per the matrix below. XCAP XCLIB NI Labview Micromanager Hawk 252 Falcon II Falcon III Eagle ...
- Page 8 Using a liquid re-circulator. The TEC and fan will allow the Eagle V to cool but if you want to run the camera at its optimum of - 95C, you will need to use a Liquid Re-circulator unit. This will help to remove the heat from the hot side of the sensor.
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Page 9: Epix Xcap
Figure 3: Open / Close 3. Select Close, and then Click on the Camera & Format button. 4. Using the dropdown menu scroll down and select Raptor Photonics Eagle V-from the list (Figure 4). Selecting OK when done. Figure 4: Camera Selection EA4240-CL and EA4710-CL / USER MANUAL Rev1.3... - Page 10 7. The control window on the right should reflect your chosen camera. For example, for the Eagle V, it should read - EPIX PIXCI E8DB: Raptor Photonics Eagle V. If not, you need to select a different camera. 8. Check that the camera is communicating, see animated icon (Figure 6) on bottom right of control screen.
- Page 11 Figure 7: Image Capture 12. You should now see an image. On the bottom of the screen, you should see something similar to the following: Frame: 0 Size: 2048 x 2048 Resize 3.4x3.4 Video: 0.4 fps Capture: 0.4 fps Display: 0.4fps. Figure 8a: Live Image Capture Window EA4240-CL and EA4710-CL / USER MANUAL Rev1.3 February 2018...
- Page 12 To adjust the image to upright, go to Resolution tab on the left and select left B-T from the drop down menu. Then click on buffer tab again. Figure 8b: Adjusted Live Image Capture Window Changing the settings on XCAP You have the ability to change several settings to control the camera through the tabs in the control window of the EPIX software.
- Page 13 Figure 9: TEC Tab Exposure Click on the Gain Tab. Figure 10: Gain Tab There is an option for high preamp gain, which is already pre-selected, offering the lowest noise. If you have a bright signal, then there is the option to deselect, offering more signal albeit with about x5 less counts.
- Page 14 Shutter Click on the shutter tab (as per Figure 11). Figure 11 Shutter Tab There are three options in the shutter control; closed, open or exposure. If you select exposure, you have to determine the shutter close delay and shutter open delay. A good test set-up to begin with is go to live, set exposure to 300msec and shutter control to exposure.
- Page 15 Use this tab to select the Trigger Mode (Live – ITR, Live - FFR, External Triggered or Button Triggered). If you select one of the triggered options, you then get a trigger polarity option (Falling edge or raising edge). To acquire a single image you use the button trigger. Click on unlive and hit the reset button. Then click on snap and then single exposure.
- Page 16 Figure 13b: AOI settings You can see how the image have focused on this particular area of interest in Figure 13b. Figure 13c: AOI Example Image EA4240-CL and EA4710-CL / USER MANUAL Rev1.3 February 2018...
- Page 17 4.10 Information Click on the Info tab (show in Figure 14) to view the cameras manufacturer data, for example Build date and serial number. Figure 14: Information Tab 4.11 Adjusting the image display (With XCAP Std only) The Viewing Pallet is not automatically adjusted in the XCAP software. This needs manually configured to give the best display of the RAW image data from the camera.
- Page 18 Figure 15: Contrast Modification Figure 16: Stretch Contrast EA4240-CL and EA4710-CL / USER MANUAL Rev1.3 February 2018...
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Page 19: Micro-Manager
MICRO-MANAGER Micro-Manager is software for control of microscopes. It works with almost all microscopes, cameras and peripherals on the market, and provides an easy to use interface that lets you run your microscopy- based experiments. Micro-Manager runs as a plugin to ImageJ, is Open Source, and is free. It is a complete image acquisition and microscope control package, available for Windows, Mac and Linux, it is easy to install and configure right "out-of-the-box", with built-in functionality and user interface for most common tasks performed in a Life Science laboratory. - Page 20 Configuring Micro-Manager When first opening Micro-Manager for the first time you will see the window shown in (figure 22). You need to make sure (none) is selected then click Ok. Figure 18: Hardware Configuration 1. Start the Hardware Configuration Wizard by going to tools then Hardware Configuration Wizard. Figure 19: Hardware Configuration Wizard EA4240-CL and EA4710-CL / USER MANUAL Rev1.3 February 2018...
- Page 21 2. Create a new configuration and add the raptor adapter from the list (shown in Figure 20), now click Next. Figure 20: Add a New Device 3. Continue to click Next until you are faced with the screen shown in Figure 20.
- Page 22 Figure 21: Save Configuration The connected camera should now be recognized. Next time you open Micro-Manager you can simply select the saved Raptor Eagle V.cfg from the drop down menu (figure 22) then click Ok. EA4240-CL and EA4710-CL / USER MANUAL Rev1.3...
- Page 23 Figure 22: Selecting Saved Camera Configurations Operating your camera using Micro-Manager Figure 23: Opening Screen EA4240-CL and EA4710-CL / USER MANUAL Rev1.3 February 2018...
- Page 24 1. Click on snap (Figure 23) for Micro-Manager to open the viewing window as shown in Figure 24. Figure 24: Viewing Window EA4240-CL and EA4710-CL / USER MANUAL Rev1.3 February 2018...
- Page 25 Changing Camera settings All of the camera parameters are controlled within the Device/Property Browser; this can be accessed by selecting Tools from the menu bar then Device Property Browser… Figure 25: Camera Control Options From here, you can access all the camera settings. EA4240-CL and EA4710-CL / USER MANUAL Rev1.3 February 2018...
- Page 26 CORPORATE HEADQUARTERS Raptor Photonics LTD Willowbank Business Park Larne, Co Antrim BT40 2SF Northern Ireland PH: +44 2828 270141 www.raptorphotonics.com EA4240-CL and EA4710-CL / USER MANUAL Rev1.3 February 2018...