Pump Base Features; Tubing Selection And Installation; Tubing Selection; Installation Of Precut Bio-Rad Tubing - BIO RAD EP-1 Econo Pump Instruction Manual

Auxiliary Output

4.3 Pump Base Features

The base of the Model EP-1 Econo Pump contains a 40-pin connector for
connection to the Model ES-1 Econo System Controller via a ribbon cable
located on the Model ES-1 Econo System Controller. When connected, the
two provide added features such as gradient proportioning and mixing, peak
detection, and control of a waste/collect diverter valve. See Section 6 for
details.
Section 5

Tubing Selection and installation

5.1 Tubing Selection

The Model EP-1 Econo Pump may be used with most flexible tubing
having an inner diameter less than or equal to 3.2 mm (l/8"), and a wall
thickness of 1.0 mm or less, including silicone, Tygon, and PharMed. Silicone
tubing, the most inert of the three, is suitable for aqueous and polar solu-
tions. Tygon is suitable for most aqueous solutions. It will generally have
the shortest lifetime of the three. PharMed is the longest lasting of the three,
and will provide the most consistent flow rate over time. Table 5.l shows
approximate flow rate ranges with different tubing sizes. Flow rates above 20
ml/min may be obtained by plumbing two channels of 3.2 mm tubing through
the pump and joining them at the output.
Table 5.1. Approximate Flow Rate Ranges
This 8-pin connector is for communi-
cation with the Model EM- l Econo
UV Monitor.
8

5.2 Installation of Precut Bio-Rad Tubing

Fig. 5.1. Tubing installation.
l. Referring to Figure 5.l, pull the platen cam lever away from the pump
head to unlock the platen and slide the platen away from the pump head
frame assembly, exposing the rollers.
2. Slip a lock-ring onto one end of the tubing. See below for the size and
color of each lock-ring. Insert a barbed female luer-fitting into the same
end until the tubing reaches the flange of the fitting. Clamp the luer-fit
ting into place by sliding the lock-ring along the tubing over the barbed
fitting (see Figure 5.2). Repeat on the other end of tubing.
Note: Use of lock-rings is required only when operating at pressures
above 10 psi.
Lock-Ring Color
Red
Orange
Yellow
9
Tubing ID
0.8 mm (1/32")
1.6 mm (1/16")
3.2 mm (1/8")