Appendix A. Approximate Gel Drying Times................... Appendix B. Specifications ....................... 10 Appendix C. Warranty and Ordering Information ................11 Figures 1. HydroTech Vacuum Pump ......................Tables 1. Front View Physical Features......................2. Rear View Physical Features ......................3. HydroTech Set Up ..........................
Bio-Rad is not responsible for any injury or damage caused by the use of this instrument for purposes other than for which it is intended or by modifications of the instrument not performed by Bio-Rad or an authorized agent.
Introduction INTRODUCTION Bio-Rad’s HydroTech Vacuum Pump is designed for use with the Model 583 Gel Dryer or any other conven- tional slab gel dryer. It uses water, not vacuum pump oil, so it is easy to use, environmentally friendly, and practically maintenance free.
2. Vacuum Line Pre-assembled with 1/4” quick disconnect fitting and 1/4” vacuum tubing. 3. Two-way Prevents foaming when the HydroTech is operating but the gel dryer is not in use. Valve This valve is plumbed into the vacuum line. 4. Power On/ This switch can be set for 0 to 3 hours of operation or continuous operation.
4. AC Power Three-prong AC power input. Connection 5. Locking Casters allow the HydroTech to be moved. Once in position, the casters can Casters be locked. 6. Drain Line Pre-assembled with 3/8” quick disconnect fittings and 3/8” tubing for draining...
Set Up and Basic Operation SET UP AND BASIC OPERATION The HydroTech Vacuum Pump is shipped fully assembled. To use the pump, simply fill the reservoir with water and attach the vacuum tubing from the gel dryer to the HydroTech’s inlet port.
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Step Procedure Description Connect the AC power cord. Plug the AC power cord into the HydroTech power input module and into a nearby AC outlet. Turn the Power On/Off switch This ensures the pump is fully primed and air bubbles to run the pump for two minutes.
Turn the vacuum line’s two-way This prevents reservoir foaming when the HydroTech valve to the Closed position. is running but the gel dryer is not in use.
MAINTENANCE AND TROUBLESHOOTING 3.1 MAINTENANCE The HydroTech pump’s reservoir water should be changed at least once a month or if frequent use of the HydroTech has caused the water level to reach MAX. Regular reservoir maintenance helps maintain pump performance and reduces foaming. To change the water, follow the steps in Table 5.
Maintenance and Troubleshooting 3.2 TROUBLESHOOTING GUIDE Refer to Table 6, Troubleshooting Guide, for HydroTech Vacuum Pump troubleshooting. For further assis- tance, call 1-800-4BIORAD in the U.S.A.; or contact your local Bio-Rad representative. Table 6. Troubleshooting Guide Action Indication Problem Check electrical source, power connections, and fuse.
Approximate Gel Drying Times APPENDIX A. APPROXIMATE GEL DRYING TIMES The following table provides approximate drying times for various gel concentrations, given the gel dryer’s temperature and vacuum are kept at 80°C and 28” Hg, respectively. Table 7. Suggested Drying Times for Single Percentage Gels Acrylamide Concentration Slab Thickness Time at 80°...
APPENDIX C. WARRANTY AND ORDERING INFORMATION The HydroTech Vacuum Pump is warranted for 1 year against defects in materials and workmanship. If any defects should occur during this warranty period, Bio-Rad Laboratories will replace the defective parts with- out charge. However, the following defects are specifically excluded: 1.
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Number Description Gel Dryer and Vacuum Systems 165-1789 HydroTech Gel Drying System, 100/120 V, includes Model 583 Gel Dryer, HydroTech Vacuum Pump, tubing and connectors. 165-1790 HydroTech Gel Drying System, 220/240 V, includes Model 583 Gel Dryer, HydroTech Vacuum Pump, tubing and connectors.
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Laboratories Life Science Web site www.bio-rad.com Bio-Rad Laboratories Main Office 2000 Alfred Nobel Drive, Hercules, CA 94547, Ph. (510) 741-1000, Fx. (510) 741-5800 Group Also in: Australia Ph. 02 9914 2800, Fx. 02 9914 2889 Austria Ph. (01) 877 89 01, Fx. (01) 876 56 29 Belgium Ph. 09-385 55 11, Fx. 09-385 65 54 Brazil Ph.
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