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Semi-Automatic Sheet Heat Sealer 4s3™
Operation Manual
Version: 1.0
4titude Ltd. • The North Barn • Surrey Hills Business Park Damphurst Lane • Wotton • Surrey • RH5 6QT
T: +44 (0)1306 884885 • F: +44 (0)1306 884886 • E: info@4ti.co.uk • www.4ti.co.uk
4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0 • en1 • 11.2018
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Summary of Contents for 4titude 4s3
- Page 1 Operation Manual Version: 1.0 4titude Ltd. • The North Barn • Surrey Hills Business Park Damphurst Lane • Wotton • Surrey • RH5 6QT T: +44 (0)1306 884885 • F: +44 (0)1306 884886 • E: info@4ti.co.uk • www.4ti.co.uk 4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0 • en1 • 11.2018...
- Page 2 • 2006/42/EC Machinery Directive Copyright Notice No part of this manual may be reproduced or transmitted in any form or by any means, electronic or mechanical, for any purpose, without prior written permission of 4titude ® Ltd. 4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0...
- Page 3 Sealed sample plates may remain hot for some seconds after sealing and should be handled with care Warning - Indicates pinch, crush and cut hazard Biohazard - Indicates the possible presence of biological or hazardous substances 4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0...
- Page 4 • To guarantee sufficient ventilation, ensure that the sealer has at least 30 cm of free space on all sides, including the rear. • Repair should be carried out by authorized service personnel only. • Use original spare parts and accessories only. 4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0...
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Page 5: Table Of Contents
8 Appendix B: Ordering Information ....................21 9 Appendix C: Warranty ........................22 10 Appendix D: Shipping Instructions .....................23 11 Appendix E: Recycling and Disposal ..................24 11.1 Uninstalling Instructions …………………………………………………………………………………………… 24 11.2 Disposal Instruction ………………………………………………………………………………………………… 24 4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0... -
Page 6: 4S3™ Introduction
4s3™ Semi-Automatic Sheet Heat Sealer 1 4s3™ Introduction The 4s3™ is a compact semi-automatic sheet heat sealer which is compatible with a wide range of seals and plates of differing designs and heights. With variable temperature and time settings, sealing conditions are easily optimised to produce a 100% tight seal, eliminating sample loss. -
Page 7: Instrument Overview
Amber LED Status Indicator Operate, Open Drawer & Close Drawer & Incremental (up) Key Decremental (down) Key 1.2.3 Rear Power Interface Power Setting 115/230 Vac and Fuse Holder I/O Power Switch Mains Power Plug 4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0... -
Page 8: Installation
The manufacturer accepts no responsibility for damage incurred unless the unit is correctly packed and transported in this way. Remove the 4s3™ unit from its packaging. Insert the power cable and power up the instrument. Once powered up, press and the drawer will open. Remove the protective elastic bands and press The unit has to be placed on a level surface, away from direct sunlight ensuring access to the power switch at the back of the unit. -
Page 9: Voltage Check
4s3™ Semi-Automatic Sheet Heat Sealer 2.2 Voltage Check Be sure that the equipment is not connected to the mains power. At the rear of the 4s3™ is the connection panel with: • Power setting 115/230 Vac and Fuse Holder • I/O Power Switch •... -
Page 10: Settings And Operation
3.1 Set-Up Operating Mode Turn on the 4s3™ (switch located at back of the unit). The display will show the software version for a couple of seconds and then show the temperature to which the 4s3™ is currently set. The unit is delivered with the heater platen turned off. -
Page 11: Operation - "Run" Mode
When using heat sealing films that have a tendency to curl, e.g. 4titude Peel Heat Seal (4ti-0521), DMSO Resistant Peel Heat Seal (4ti-0587) and Clear Weld Heat Seal Mark II (4ti-0575), use the 4s3™ Sealing Frame (4ti-0612) to hold down the seal. -
Page 12: Power-Down" Mode
4 Optimising Seal Quality 4.1 Plate Requirements The 4s3™ accommodates plates made from a variety of materials and plate designs. For a complete list of the acceptable sealing materials, please reder to section 4.3, page 16. The 4s3™ is designed to accommodate plates which meet the standard established by the Society of Biomolecular Sciences (SBS). -
Page 13: Plate Support Adapters
4.2 Plate Support Adapters The 4s3™ is designed to seal microplates from 4 mm to 60 mm in height. All plates must first be placed in an appropriate adapter to bring the sealing surface into the correct height range for the sealer. In addition to this, 96 and 384 well PCR plates require an adapter to support the plate precisely enough to produce an even seal. -
Page 14: Heat Seal Materials
4s3™ Semi-Automatic Sheet Heat Sealer 4.3 Heat Seal Materials The 4s3™ is compatible with the widest range of heat sealing materials available on the market. Details of available ® ® materials can be found in the following table, 4titude literature, or by visiting the 4titude website www.4ti.co.uk. - Page 15 4.4.1 Sealing Parameters Note: These values are for guidance only. It is advisable to conduct trials depending on the plate type being sealed. Add 1 second to the sealing time when the 4s3™ Weighted Sealing Platen is used (4ti-0602) Sealing temperature (°C) / Dwell time (sec)
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Page 16: Troubleshooting
5 Troubleshooting A list of possible error messages generated by the 4s3™ are summarised within this chapter. If the solutions given below do not solve the problem, please contact 4titude at instruments@4ti.co.uk or their authorised local distributor 5.1 Error Messages... -
Page 17: Maintenance
• To allow better access to heating platen, lay the unit gently onto the side 4s3™ placed onto the side to give easier access to heating platen • Clean heating platen using a blunt plastic tool, such as a spatula. Gently scrape away the hardened plastic •... -
Page 18: Appendix A: Technical Specifications
4s3™ Semi-Automatic Sheet Heat Sealer 7 Appendix A: Technical Specifications Parameter Specification Mechanical Dimensions (H x D x W) 313 mm x 277mm x 181 mm Weight 10 kg Electrical Voltage 00-115/230 ±10% Vac HT121TS_4B_en_d.doc Page 12 of 35 Frequency 50/60 Hz Power at 100-115V... -
Page 19: Appendix B: Ordering Information
Includes: Power cord, manual, standard plate support adapter (4ti-0615), 12 months warranty 4ti-0602 4s3™ Weighted Sealing Platen 4ti-0612 4s3™ Sealing Frame For use with 4s3™ Plate Support Adapter, PCR 96 (4ti-0625) 4ti-0613 4s3™ Plate Support Adapter, Random Access, Low Profile For use of Random Access Seals with low profile plates 4ti-0614 4s3™... -
Page 20: Appendix C: Warranty
® Ltd‘s Quality Control System guarantees that the performance of the 4s3™ Heat Sealer you have purchased is within its specifications. The warranty covers all parts and labour costs associated with a repair of the unit within the first 12 months. The need for returning a unit for service must first be agreed with 4titude ®... -
Page 21: Appendix D: Shipping Instructions
5. Place the sealer between the two foam inserts inside the original shipping box. 6. Place the accessories in the smaller cardboard box and place on top of the foam inserts. 7. Close the box using a suitable adhesive tape. 4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0... -
Page 22: Appendix E: Recycling And Disposal
4s3™ Semi-Automatic Sheet Heat Sealer 11 Appendix E: Recycling and Disposal For what concerns sealer recycling and disposal, the following operations have to be carried out: switch off the sealer, remove the plate, eliminating any contam-ination on the instrument in accordance with national and international safety regulations.
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4S3(セミオート・ヒートシーラ―)はPCR Plate, 96-well, segmented, semi-skirted（AB0900, Thermo Scientific）を使えますでしょうか。
The 4titude 4S3 semi-automatic heat sealer can seal 96-well PCR plates, including those made from polypropylene and with semi-skirted designs, if used with the appropriate plate support adapter that fully supports each well. Therefore, it can be used with the Thermo Scientific AB0900 PCR Plate, 96-well, segmented, semi-skirted, provided a suitable adapter is used.
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