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Nikon C1si User Manual

Spectral laser scanning confocal microscope
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Spectral Laser Scanning Confocal Microscope
Contents:
C1Si Turn-On/ShutDown Procedures ............................................................................................. 2
Overview ......................................................................................................................................... 4
Setup for epi-illumination to view through the eyepieces: ............................................................... 5
Setup for confocal imaging:............................................................................................................. 5
Notes:.............................................................................................................................................. 6
Troubleshooting .............................................................................................................................. 7
Owners Consortium:
Department of Pathology
SABRE
Proctor Foundation
Nikon C1si
User Guide
Page 1 of 7
 
 
 
 
 
 

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Summary of Contents for Nikon C1si

  • Page 1: Table Of Contents

    Nikon C1si Spectral Laser Scanning Confocal Microscope User Guide Contents:   C1Si Turn-On/ShutDown Procedures ..................... 2   Overview ............................4   Setup for epi-illumination to view through the eyepieces: ............... 5   Setup for confocal imaging:......................5   Notes:.............................. 6  ...

  • Page 2: C1Si Turn-On/Shutdown Procedures

    C1Si Turn-On/ShutDown Procedures Turn On: 1. Turn on arc lamp (Nikon Intensilight on shelf above table). This should be left on for at least 30 minutes at a run and turned off for at least 30 minutes prior to turning on again, this prevents the arc from flickering and wearing out prematurely 2.
  • Page 3 7. Turn on computer: 8. Launch software (EZ-C1 3.80). a. Choose the colors you are going to use: 408/488/561, 488/561/638, or 457/514 (If you plan on doing spectral imaging it doesn’t matter what you choose in the software). 9. Choose standard or spectral detector. a.

  • Page 4: Overview

    32 different channels at the same time (they can be set to be 2.5nm, 5nm, and 10nm wide). It is on an upright Nikon FN1 microscope with a large electrophysiology stage. The max penetration depth into tissue is approximately 100–200 µm depending on the opacity of the sample.

  • Page 5: Setup For Epi-Illumination To View Through The Eyepieces

    Setup for epi-illumination to view through the eyepieces: 1. Choose dichroic for appropriate fluorophore. 2. Open shutter at epi source and shutter on front of scope (move to ‘O’ from ‘C’). 3. Turn off brightfield. 4. View sample through the eyepieces. Filters for the Eyes Fluorophore Cube D/F/R...

  • Page 6: Notes

    b. Set a reference point (any point in between your top and bottom) c. Set the top by either engaging the stepper motor and focusing up or entering in a number. d. Set the bottom same as the top. e. Set step size (the N button sets Nyquist-Shannon Sampling which is 2.7 times the theoretical resolution limit) Now click the green check box in the top window so when you click single it takes the whole z-stack (if you do this beforehand it will take a z-stack...

  • Page 7: Troubleshooting

    Troubleshooting The z stepper motor is not working •  The Make sure that the knob is pulled up on the rear left of the microscope. If   y ou cannot see anything in the eyes (epi) follow this checklist:     •...