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Summary of Contents for Antec Scientific FlexCell
- Page 1 Antec Scientific Industrieweg 12 2382 NV Zoeterwoude The Netherlands FlexCell Electrochemical flow cell User manual 102.0010A, Edition 9, 2017 T +31 71 5813333 | info@ AntecScientific.com | https://AntecScientific.com...
- Page 2 Information in this publication is subject to change and does not represent a commitment on the part of Antec Scientific. Antec Scientific reserves the right to make changes to the design and specifications of the instrument and the contents of this manual without prior notice.
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Page 3: Symbols
Symbols The following symbols are used in this guide: The warning sign denotes a hazard. It calls attention to a procedure or practice which, if not adhered to, could result in severe injury or damage or destruction of parts or all of the equipment. - Page 4 FlexCell user manual, edition 9 Intended use The FlexCell is used in combination with an Antec Scientific Electrochemical Detector (for example DECADE, INTRO, DECADE II or DECADE Elite/Lite in (Ultra) High Performance Liquid Chromatography for the electrochemical detection of suitable analytes in liquid samples. (U)HPLC is a method for separating substance mixtures, determining substances and measuring their concentration.
- Page 5 Only use the device for applications that fall within the scope of the specified intended use. WEEE directive All equipment of Antec Scientific which are subjected to the WEEE directive shipped after August 13, 2005 are compliant with the WEEE marking requirements. Such products are labelled with the “crossed out wheelie”, depicted on the left site.
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Page 6: Safety Instructions
FlexCell user manual, edition 9 Safety instructions Adhere to the following guidelines when using the electrochemical flow cell. These safety practices are intended to ensure safe operation. This hardware should be used by trained laboratory personnel only with a completed degree as chemical laboratory technician or comparable vocational training. - Page 7 If necessary the flow cell must be decontaminated before decommissioning or shipment of the cell for repair to Antec Scientific or its representatives. When shipped to Antec Scientific every cell has to be accompanied with a decontamination form which should be completely filled in and signed by the customer.
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Page 8: Spare Parts And Service Availability
FlexCell user manual, edition 9 Disposal program. Never dispose of flammable and/ toxic solvents through the municipal sewage system. Applications: quality control It is recommended that you routinely run several quality control samples. Quality control samples should represent low, average and high levels of a compound. -
Page 9: Table Of Contents
CHAPTER 2 ....................17 General precautions ..................17 CHAPTER 3 ....................18 Installation ..................... 18 Connecting a FlexCell to an LC system ........... 18 CHAPTER 4 ....................20 Maintenance ....................20 Disassembly of the flow cell .............. 20 Working electrode cleaning .............. 22 Polishing a working electrode ............ - Page 10 FlexCell user manual, edition 9 CHAPTER 5 ....................29 Specifications....................29 CHAPTER 6 ....................30 Part list ......................30...
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Page 11: Chapter 1
The electrochemical flow cell Introduction The FlexCell™ (Fig. 1) has been developed for analysis in standard and microbore LC-EC with an effective volume of only 0.5 µL. A range of different working electrode materials are available for the FlexCell and they are easily exchangeable, offering maximum flexibility for running various applications. - Page 12 FlexCell user manual, edition 9 voltage can be processed by an integrator, recorder or AD convertor to generate the chromatogram. Fig. 2. Schematic representation of an electrochemical cell with a three- electrode configuration. Essentially, for the oxidation or reduction reaction it would be sufficient to use only two electrodes.
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Page 13: Requirements And Limitations
Ideally, a high signal is obtained at a low working potential as noise levels increase with potential. For the FlexCell, different working electrode materials are available: glassy carbon, gold, silver, platinum, Boron Doped Diamond (BDD), and copper. For most regular applications, glassy carbon will be the working electrode material of choice. - Page 14 Boron Doped Diamond (BDD) electrodes Boron Doped Diamond (BDD) is the most recent addition to the list of available FlexCell working electrode disks. The BDD electrode consisting of an ultra-thin film of boron doped diamond material deposited on a Si-wafer.
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Page 15: Reference Electrode: Hyref
CHAPTER 1 The electrochemical flow cell Reference electrode: HyREF™ The is most commonly used reference electrode for a FlexCell is the maintenance-free HyREF. This reference electrode is most suitable for use with high concentrations (>20%) of organic modifier in the mobile phase or when running alkaline mobile phase. -
Page 16: Reference Electrode: Salt Bridge Ag/Agcl
FlexCell user manual, edition 9 Reference electrode: salt bridge Ag/AgCl The salt bridge Ag/AgCl reference electrode consists of a small container, with a solid AgCl coated silver rod immersed in a solution of saturated KCl (Fig. 4). This type of reference electrode needs regular maintenance. -
Page 17: Reference Electrode: Isaac
CHAPTER 1 The electrochemical flow cell Reference electrode: ISAAC™ The In Situ Ag/AgCl (ISAAC) reference electrode is the low-maintenance version of the Ag/AgCl saltbridge reference electrode. The potential of an ISAAC is defined by the concentration of chloride ions in the mobile phase. - Page 18 FlexCell user manual, edition 9 Table II. Potential of the Ag/AgCl reference electrode. A saltbridge Ag/AgCl reference is loaded with saturated KCl and the ISAAC is in contact with the concentration of chloride added to the mobile phase. dE is the potential...
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Page 19: Chapter 2
5. If the flow cell is not in use and removed from the LC system, we recommend that you disassemble the cell and clean all surfaces. The construction of the FlexCell is such that both fluid connections can be used as in- or outlet. -
Page 20: Chapter 3
LC-EC trace analyses. Connecting a FlexCell to an LC system 1. Install a suitable length of sharply cut 1/16” OD PEEK tubing to the column outlet. Choose a tubing ID that matches the column ID: 0.25 mm ID for working with normal bore columns... - Page 21 CHAPTER 3 Installation Remove the saltbridge storage cap and tighten the black swivel a little bit extra, which should result in a small droplet appearing from the frit. This droplet indicates that the frit is not dried out or clogged. Leave the droplet on the tip when inserting it into the reference chamber, as it ensures proper contact of the REF with the mobile phase.
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Page 22: Chapter 4
Before disassembling the flow cell, read the General Precautions. Disassembly of the flow cell 1. Switch the flow cell off, set the flow rate to zero, and take the FlexCell out of the system after disconnecting both finger-tight connectors from the cell. - Page 23 (1) and held in place by a silicon electrode holder (2). Hy-REF reference electrode auxiliary electrode inlet block retaining ring 50 µm spacer working electrode disk Working electrode assembly electrode swivel nut Fig. 8. Exploded diagram of FlexCell.
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Page 24: Working Electrode Cleaning
FlexCell user manual, edition 9 Working electrode cleaning A typical reason to apply maintenance to the working electrode is when the flow cell performance decreases. In most cases, a simple cleaning is sufficient to remove the chemical deposition that is causing the problem: 1. -
Page 25: Flattening A Metal Working Electrode
Metal working electrodes may require a more thorough flattening step as some specific conditions result in the consumption of the wetted material. For metal working electrode disks of the FlexCell type, there is a dedicated flattening and polishing kit available (not standard provided with a FlexCell). -
Page 26: Salt Bridge Ag/Agcl Reference Electrode Check
FlexCell user manual, edition 9 Fig. 9. Salt bridge Ag/AgCl reference electrode, assembled (bottom) and disassembled (top). The yellow cap prevents drying out of the cotton frit that is mounted in the tip of the body. See Table III for numbers and descriptions. -
Page 27: Refilling A Salt Bridge Ag/Agcl Reference Electrode
CHAPTER 4 Maintenance 2. the background current will increase, 3. sensitivity for movements and pump noise will increase. Wet crystals can be visible as a transparent deposition in the body. Holding the REF upside down makes the crystals better visible, as the deposition will move downward through the REF body. -
Page 28: Refritting A Salt Bridge Ag/Agcl Reference Electrode
FlexCell user manual, edition 9 5. Completely fill up the body with saturated KCl solution 6. Place the O-ring on top of the open body 7. Insert the electrode, making sure not to trap air under the cap. It may be necessary to wiggle the electrode to accommodate it between the crystals. -
Page 29: Assembly Of The Flow Cell
CHAPTER 4 Maintenance Fig. 10. Pushing the cotton wool frit out with the sb REF tool. 5. Clean all parts with demi-water. Inserting a new cotton wool frit 1. Place the salt bridge with the frit-hole downwards on a glass plate and add a few drops of saturated KCl solution 2. -
Page 30: Storage
FlexCell user manual, edition 9 To prevent the spacer from slipping off the pins, keep the flow cell oriented as illustrated in Fig. 11 while mounting the retaining ring and WE assembly. 5. Install the working electrode assembly and check that the groove in the assembly aligns with the internal pin (which prevents it from turning). - Page 31 CHAPTER 5 Specifications C H A P T E R Specifications Cell type Three electrode, thin-layer flow cell approx. 0.7 μL (50 μm spacer), Cell volume Spacers 50 µm or 130 µm Working electrode diameter 8 mm Working electrode area 15 mm (wetted) Working electrodes (WE)
- Page 32 FlexCell user manual, edition 9 C H A P T E R Part list In the table below parts and spare parts are listed for the FlexCell: Description FlexCell parts 102.2205 FlexCell inlet block 102.0912 WE holder assembly for FlexCell 102.2217...
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