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Related Manuals for Beckman Coulter Biomek 3000
Summary of Contents for Beckman Coulter Biomek 3000
- Page 1 ® Biomek 3000 Laboratory Automation Workstation Quick-Start Guide Beckman Coulter PN 987837 Revision AB September 2004 Beckman Coulter, Inc 4300 N. Harbor Boulevard, Fullerton, CA 92834-3100 Copyright © 2004 Beckman Coulter, Inc. Printed in U.S.A.
- Page 2 Except as expressly provided herein, Beckman Coulter, Inc. makes no other warranty, whether oral or written, expressed or implied, as to any matter whatsoever, including but not limited to those concerning merchantability and fitness for a particular purpose, nor is freedom from any patent owned by Beckman Coulter, Inc. or by others to be inferred.
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Page 3: Warranty And Returned Goods Requirements
Warranty and Returned Goods Requirements All standard Beckman Coulter, Inc. policies governing returned goods apply to this product. Subject to the exceptions and upon the conditions stated below, the Company warrants that the products sold under this sales agreement shall be free from defects in... -
Page 5: Table Of Contents
1.4.1 Turning On the Biomek 3000 ....... . . 1-7 1.4.2... - Page 6 Quick-Start Guide Summary ........3-19 Beckman Coulter, Inc.
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Page 7: Introducing The Biomek® 3000 Laboratory Automation Workstation
® • Getting to Know the Biomek 3000 Laboratory Automation Workstation (this chapter) — a general hardware overview on the Biomek 3000 Automated Laboratory Workstation and introduction to the Biomek Software. • Learning to Create Methods for the Biomek® 3000 (Chapter 2) —... -
Page 8: How To Use This Quick-Start Guide
Moving back and forth between this guide and the user’s manuals you will be able to begin to learn about the Biomek 3000 instrument and software and also become familiar with the manuals and how chapters are arranged. -
Page 9: Biomek® 3000 Laboratory Automation Workstation
The Biomek 3000 is a single head instrument with a series of interchangeable tools. Different tools provide options for performing a variety of functions, including liquid transfer and plate washing operations and moving labware around the deck. The... -
Page 10: Head Assembly
Labware positioners are used to hold microplates, tube racks, reservoirs, or tip racks on the Biomek 3000 deck. The gripper tool can move labware to and from these labware positioners to other labware positioners or devices integrated on the left or right side of the Biomek 3000 workstation. -
Page 11: Optional Devices
• Stacker Carousel — provides additional labware capacity for microplates and tip boxes to the Biomek 3000 workstation. It includes up to four stacks and a shuttle for transporting labware between the Biomek 3000 deck and the Stacker Carousel. A Stacker Carousel may be installed on either the left side, right side, or both sides (refer to the Biomek®... -
Page 12: Biomek Software
Allow you to take as much direct and precise control over the method- building process as you want. Note: A method is a series of steps controlling the operations of your Biomek 3000. The main editor that appears when the software is launched (Figure 1-2) is used to build and configure methods: •... -
Page 13: Starting The Biomek® 3000
Introducing the Biomek® 3000 Laboratory Automation Workstation ® Starting the Biomek 3000 To start the Biomek 3000, you will follow the instructions in this chapter to turn on Accounts & the instrument, launch Biomek Software, and home all axes. Permissions When enabled, Accounts 1.4.1... -
Page 14: Homing All Axes Of The Biomek® 3000 Pod
1.4.3 Homing All Axes of the Biomek 3000 Pod Every time the Biomek 3000 is turned on, you’ll have to Home All Axes. This Home All Axes automatic process initiates the pod and defines the home position from which all subsequent moves are determined. -
Page 15: Learning To Create Methods For The Biomek® 3000
Biomek 3000. The method you complete in this chapter represents basic operations and is intended simply to familiarize you with the Biomek 3000, and to create a basic understanding of method-building opportunities. Read the information in this chapter and complete the tutorial steps provided. After you have completed the instructions, or if you need additional information along the way, use the links provided to access the suite of user’s manuals provided during... -
Page 16: Creating A New Method
Move labware with the Gripper Tool using a Move Labware step. the Biomek Software User’s Manual, Chapter 2, Note: A gripper tool rack must be installed on the Biomek 3000 deck to Using Accounts & store a gripper tool when not in use. Permissions, for more information. - Page 17 Figure 2-1. Biomek Software main editor for a Biomek 3000 instrument Note: Your deck layout may differ slightly from the deck shown in Figure 2-1, depending on how your Biomek 3000 instrument is physically set up.
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Page 18: Configuring The Instrument Setup Step
If this is not done, the pod could collide into these objects as it moves around the deck during a method run. Figure 2-2. Instrument Setup step in the Method View Beckman Coulter, Inc. - Page 19 Learning to Create Methods for the Biomek® 3000 To configure the Instrument Setup step for your new method: 1. In Deck, make sure the default deck layout (Deck1) is selected. The Instrument Setup step uses a deck layout as a map for the placement of labware on the deck and external hardware devices, such as stacker carousels.
- Page 20 5. Drag the MP200 tool to either of the two remaining tool positions. 6. Choose OK to close Labware Properties. 7. From the Deck Layout Display, double-click the reservoir on P3 to display Labware Properties (Figure 2-5). Figure 2-5. Labware Properties for Reservoir on P3 Beckman Coulter, Inc.
- Page 21 Learning to Create Methods for the Biomek® 3000 8. In Name, enter Resv in Labware Properties. Naming Labware 9. In Labware contains, select Known from the pull-down menu. It is helpful to name 10. In volume, enter 100000. This means that you know you have 100000 labware, particularly microliters of liquid in the reservoir.
- Page 22 Instrument Setup Step. Figure 2-6. Instrument Setup step with labware named Next you will configure a Transfer step using an MP200 tool. If your instrument configuration does not include an MP200 tool, any pipetting tool may be substituted. Beckman Coulter, Inc.
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Page 23: Configuring A Transfer Step
Learning to Create Methods for the Biomek® 3000 Configuring a Transfer Step The Transfer step transfers liquid from a single source to one or more destinations. Transfer Step You are going to insert a Transfer step into the method and configure it to move liquid from your source reservoir (Resv) to your destination microplate (Dest1) A Transfer step loads using an MP200 Multi-Tip Pipette Tool. -
Page 24: Configuring Tip Handling
Transfer step, a tool can be required because the found on the Biomek loaded by inserting a Load Tool step prior to the Biomek 3000 locates and 3000 Step Palette and is Transfer step. For this quick-start method, it is not loads clean tips... -
Page 25: Configuring Source Labware
Learning to Create Methods for the Biomek® 3000 2-11 2.4.3 Configuring Source Labware To configure the labware named Resv as source labware in the Transfer step: 1. Click on Click here to add a source. 2. Click on Resv labware sitting on P2 in the Current Deck Display. Note: The information you supplied during Instrument Setup is displayed in the source labware configuration. -
Page 26: Configuring Destination Labware
Note: If you accidentally open too many destination configurations, all you have to do is right-click on the title above the graphic of the plate in the configuration. Click on Delete from the popup menu and the entire configuration goes away. Beckman Coulter, Inc. - Page 27 Learning to Create Methods for the Biomek® 3000 2-13 The destination labware is now configured, and the main editor looks like Figure 2- 10. The Transfer step is now configured to transfer 100 µL from the source reservoir Resv to the destination microplate Dest1. Caption changes to reflect the amount transferred...
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Page 28: Configuring A Move Labware Step
Biomek 3000 instrument deck to another position. the Biomek 3000 Move Labware can also be used to remove labware workstation to grasp and... -
Page 29: Using The Finish Step
Learning to Create Methods for the Biomek® 3000 2-15 Using the Finish Step Your liquid transfer is set up, so you can see how long it will take to run the entire method by using the Finish step. 1. Click on the Finish step in the Method View. 2. -
Page 30: Saving A Method
Files. 2. Enter Quick-Start Method and choose OK. The method editor now looks like Figure 2-14. Figure 2-14. Quick-Start method saved and named Move on to Section 2.8, Running a Method, to run your saved Quick-Start method. Beckman Coulter, Inc. -
Page 31: Running A Method
Learning to Create Methods for the Biomek® 3000 2-17 Running a Method Next you will run the method you just created using the simulator. The simulator allows you to watch the method on your screen without actually running the method on an instrument. - Page 32 4. Click on the green arrow button on the toolbar. The simulation display is automatically launched and the step configuration area is replaced with a three- dimensional model of your Biomek 3000 (Figure 2-16). The deck confirmation prompt (Figure 2-17) appears on top of the main editor.
- Page 33 Learning to Create Methods for the Biomek® 3000 2-19 5. Choose OK. The prompt closes and the method continues. View the method on screen as it proceeds in the simulator. The method can also be followed in the Method View as steps are highlighted in green as they are executed. Note: If you are running this method on your instrument instead of the simulator, make sure that the physical deck layout matches the expected deck layout shown in the deck confirmation prompt before you choose OK.
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Page 35: Using The Editors While Creating Methods
Using the Editors While Creating Methods Overview This chapter presents step-by-step information to help you learn how to use the editors. You can follow the instructions to use the editors or read through this chapter without performing the numbered steps. Links to specific sections offer complete information on all the editors and allow you to use this chapter in conjunction with the Biomek Software User’s Manual. -
Page 36: General Description Of Editors
Using the Editors While Creating Methods General Description of Editors Several editors are used to configure Biomek Software to control your Biomek 3000 If using Accounts & instrument. Editors are accessed from one of two menus: Permissions . . . -
Page 37: Project Menu
Managing the Deck). • Device Editor — external devices may be integrated to your Biomek 3000 instrument. In order to use the device, it must be configured through the Device Editor (refer to the Biomek Software User’s Manual, Chapter 22.4.1, Configuring Devices Using the Device Editor). - Page 38 Z-Max to raise it to the highest configured height. Import/Export Utility — allows you to import and export instrument settings • (decks, framing tools, pod settings) as import files (.imp) (refer to the Biomek Software User’s Manual, Chapter 3, Using Instrument Files and Settings). Beckman Coulter, Inc.
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Page 39: Creating New Labware In The Labware Type Editor
Using the Editors While Creating Methods Creating New Labware in the Labware Type Editor The Labware Type Editor is used to create labware definitions so you can use Labware labware that is not predefined in Biomek Software in your methods . Definitions Assume that you have new labware that is very similar to a BCFlat96, but some When creating a new... - Page 40 3. Enter NewLabwareQuickStart and choose OK. The copy of BCFlat96 is created with the specified name and appears in Labware Type Editor (Figure 3- Copy of BCFlat 96 renamed to NewLabwareQuickStart Figure 3-4. NewLabwareQuickStart in Labware Type Editor Beckman Coulter, Inc.
- Page 41 Using the Editors While Creating Methods 4. Double-click the NewLabwareQuickStart graphic. NewLabwareQuickStart appears (Figure 3-5). Figure 3-5. NewLabwareQuickStart Note: Each piece of labware in Labware Types has its own mini-editor similar to Figure 3-5 to allow specifications to be revised and saved. 5.
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Page 42: Creating A New Pattern
NewLabwareQuickStart labware you created in the Labware Type Editor. To create a new well pattern in the Well Pattern Editor: 1. From the Project menu, choose Well Pattern Editor. Well Patterns appears (Figure 3-6). Figure 3-6. Well Patterns Beckman Coulter, Inc. - Page 43 Using the Editors While Creating Methods 2. Choose New. New Pattern appears (Figure 3-7). Figure 3-7. New Pattern 3. Highlight NewLabwareQuickStart and choose OK . Figure 3-8 appears. Figure 3-8. Well Patterns based on Pattern 96 Biomek® 3000 Laboratory Automation Workstation Quick-Start Guide...
- Page 44 4. While holding down the Shift key, select the wells for the new pattern so it looks like Figure 3-9. Figure 3-9. Wells chosen for the new pattern 5. Choose Rename to rename the new pattern. Rename Pattern appears (Figure 3-10). Figure 3-10. Rename Pattern Beckman Coulter, Inc.
- Page 45 Using the Editors While Creating Methods 3-11 6. Enter LabwareQuickStart and choose OK. Figure 3-11 appears. Figure 3-11. New pattern renamed 7. Choose Save to save LabwareQuickStart in Well Patterns. Note: Refer to the Biomek Software User’s Manual, Chapter 11, Creating Well Patterns, for more information on creating well patterns.
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Page 46: Checking In A Method And Project File
This ensures that validated method runs are reproducible. Refer to the Biomek Software User’s Manual, Chapter 12.15, Checking Out a Method. Beckman Coulter, Inc. -
Page 47: Using Hardware Setup
3-13 Using Hardware Setup While a Beckman Coulter Service Engineer normally installs and configures new devices in Hardware Setup, it may be necessary for you to install, configure, and remove other devices using Hardware Setup if you change the configuration of your instrument. - Page 48 Note: The other fields may be used to modify the performance of the tool. However, Tool Conversion Slope and Capacity should only be changed if you are instructed to do so by a Beckman Coulter support or service representative. Refer to the Biomek® 3000 Laboratory Automation Workstation User’s Manual, Chapter 12, Configuring the Biomek®...
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Page 49: Creating A New Deck
A deck in the Biomek Software refers to the software representation of the physical Framing Biomek 3000 instrument deck. The Deck Editor is used to define and change deck configurations. A deck stores framing information and device associations for each Framing teaches Biomek position on the deck. - Page 50 Figure 3-18. Select a Deck 7. Choose Deck1 to make it the default deck since it is the deck you used when you created the Quick Start Method and choose OK. 8. Choose Save to exit the Deck Editor. Beckman Coulter, Inc.
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Page 51: Manually Controlling The Pod
3-17 Manually Controlling the Pod If you need to control the Biomek 3000 instrument outside of a method, including moving the bridge and head assembly outside of a method, you will use Manual Control. Manual Control is also used to control the pod when framing the gripper tool and when recovering from errors. - Page 52 2. Select Advanced Controls and highlight Pod1. Advanced Manual Control: Pod1 appears (Figure 3-20). Figure 3-20. Advanced Manual Control for the Biomek 3000 head assembly 3. Select Move Z-Max. The head assembly moves to the highest configured height. 4. Select Close. Advanced Manual Control: Pod1 closes.
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Page 53: Quick-Start Guide Summary
Biomek 3000. If you’ve used the links to other manuals, you’ve also learned where to find hardware information on the Biomek 3000 and more information on Biomek Software. Enjoy your new Biomek 3000 Laboratory Automation Workstation.