Getting Started; Easy Installation; Starting The Software; User Profiles - Jenoptik PROGRES GRYPHAX SUBRA Safety & Operating Instructions

Quick-Start Guide for PROGRES GRYPHAX Microscope Cameras

Getting Started

Congratulations on purchasing your PROGRES GRYPHAX microscope camera! This Quick-Start Guide will help you to quickly install your
PROGRES GRYPHAX® microscope camera and imaging software, and will give you an overview over their main functions.
For more information and user training, please see our detailed software manual and use our video tutorials:
https://www.jenoptik.com/en-progres-gryphax-software
You can also find these video tutorials and software manual on the USB memory card included with your camera.
For the safe operation of your PROGRES GRYPHAX microscope camera, please observe the safety and operating instructions as well as the
hardware requirements described on the reverse of this page.
Important note: Only use accessories which are included with your camera or which are explicitly recommended by Jenoptik.

Easy Installation

• Mount your PROGRES GRYPHAX camera to the microscope using the C-Mount adapter
• Turn on the main power switch
• Open Windows Explorer and run the installation application, by following the USB path (USB memory card included with your camera)
and follow the wizard to install the PROGRES GRYPHAX software. (Administrator permission are necessary for successful installation)
• Connect the PROGRES GRYPHAX microscope camera to your computer using the USB 3.0 cable.
Power is supplied via this cable; so you do not need to connect to any further equipment.
• Ensure that the illumination of your microscope is switched on and the light path is opened

Starting the Software

Click on the Jenoptik application icon on your desktop to launch the PROGRES GRYPHAX software:
By default, a grey-balanced and well-exposed live image will appear in "Fit to screen mode" and you can immediately start working with
the application software.
Panorama / Image Stitching
(in live mode)
... captures multiple images and stitches these together automatically
into one single high resolution image. You can activate it by pressing this:
Then press "REC" to start recording a panorama image. The Image
window shows a ~60% scaled-down image version to help you to adjust
your sample. While you do this and select the area that you are interested
in, all single images will be automatically stitched together. If you are
satisfied with the stitched result, just press "STOP" to save the final
stitched image, which will then appear in the Gallery.
For more details see our software manual.
Z-stacking / Multifocus / EDF
(in live mode)
... records a series of single images automatically, each in a different
depth of focus, and stitches these together to one final image that is
clearly focused in all areas. You can activate it by pressing this:
Then press "REC" to record a z-stacking image. During "REC" mode you
can manually adjust the focus of your specimen in the z-axis at your
microscope. The single image and the image result preview are compared
and viewed side by side. If you are satisfied with the result, just press
"STOP" to save the compound image and the EDF image will appear in
the Gallery.
For more details see our software manual.
Time-lapse / Image Series
(in live mode)
... records an image series (a fixed number of images during a specified
time-period or within a given timeframe).
You can activate it by pressing this:
Then press "REC" to start recording the time-lapse image series.
Recording mode automatically stops after all images of the image series
that were previously defined have been recorded. The image series will be
stored in a separate sub folder under the used image destinations folder.
For more details see our software manual.
Video
(in live mode)
... records a video sequence of images.
You can activate it by pressing:
Then press "REC" to start recording the video sequence.
Clicking "STOP" will end the recording
(The video is saved to the user-defined storage location).
Video playback, to observe the recorded videos, are possible from the
Gallery. For more details see our software manual.
Multi-Fluorescence
... captures single or multi-coloured fluorescent images (through capturing
and automatic merging of monochrome and bright-field images).
You can activate it by pressing this:
The multi-fluorescence panel and a coloured grey-scaled version of
the live image will be opened and displayed within the Image window.
Note: You can adjust the current image using tools of Multi Fluorescence
widget. You can add and modify your fluorescence filters, with
predefined colours according to the list of emission wavelengths, just press
"Add Filter". With the colour selector besides each filter, the emission
wavelengths can be changed. To delete a filter, just select it and click on the
trash icon in the fluorescence tool.
To apply the created filters to the image, click on a blank filter, surrounded
only by a thin red border and the live image gets filled with the selected colour. After pressing "REC" the captured image will be
shown within the filter frame. Repeat the previous steps for all predefined filters. The composite preview image at the bottom of the
filter list will be updated on each filter capture. Please double click on the preview image to display it within the Image window. If you
are satisfied with the composite image, just press "REC" to save the final multi-fluorescent image to the Gallery. After the final version
is saved you can start capture new filter images. The predefined filters are available for a new multi-fluorescent capture.
For more details see our software manual.
Histogram
(in live mode )
... displays the colour and alpha value distribution within the currently
viewed image, in real-time. For more details see our software manual.
Effects / f(x)
(in live mode)
... enhances the live image in order to improve the final captured image.
You can individually adjust sharpness, as well as dynamic and noise
characteristics, just by moving the sliders from the left ("off"), over "soft"
and "middle" to the right ("strong"). For more details see our software
manual.

User Profiles

... users can save your individually created calibrations and settings in order
to make your microscopic imaging reproducible / trackable. To do this, just
choose "User Profile" in the menu bar and press "Save as". You can protect
your profiles by password. For more details see our software manual.
Preferences
Your software setting preferences can be adjusted according to your individual needs. (all advanced settings here are
described in our software manual or at the video tutorials):
 Language, Minimize gain during capture, GUI scaling, Magnifier zoom factor
 Set & Calibrate black shading, Activate cooling
 Auto Save, Image format, Image quality, File name, Destination folder
 Image resolution, Color temperature, Calibrate Measurement, Calibrate White shading
 Settings for the Status bar, Scale bar unit
 Font, Style, Colour, Width, Grid colour

Capturing a Microscope Image in just One Step (in single capture mode)
The following image capture parameters are preset using the one-click
image capture function:
 Auto exposure: activated by default. Exposure options can be
individually adjusted using the Exposure tool.
 White balance: preset / adjusted to halogen lamp HAL100 (Zeiss).
Shading options can be created via "Preferences".
 Image resolution: the settings are preset, but can be adjusted under
"Preferences" >"Device Configuration"
 Target folder / Image name / File format / Language: these settings
are preset but can be individually set under "Preferences"
Further image enhancements, settings and tools have to be applied
before pressing "REC". To do this open the Gallery bar / Tool bar by clicking here:
General note: After clicking "REC", the "REC" button instantly changes to "STOP" or "LIVE" to indicate, that the captured image
is saved and the Offline mode is activated. During Offline mode only a few additional tools can be activated.
Options: You can predefine the following three settings for Offline mode after pressing "REC"
 the captured image is displayed for 3 sec., afterwards it automatically changes back into Live image mode (this option is preset)
 you can change the length of time that the captured image is displayed
 you can predefine when you will reenter Live mode after pressing the "LIVE" button again and to capture additional images
Furthermore, PROGRES GRYPHAX software gives you the opportunity to further improve or modify the image before capturing it.
See the following instructions or for detailed information read software manual available on USB memory card included by camera.
Measurements
... creates different measurements in live and captured images.
You can activate it by pressing this:
You can choose which measurement element you would like to use
(distance, freeform, radius, diameter, angel) as well as the font, outline
width and colour settings of the measurement elements. The
measurement automatically appears after clicking in the relevant place.
Measurement calibration in advance is necessary!
For more details see our software manual.
Arrow Marking
... inserts arrow markings into the image to highlight points of interest.
You can activate it by pressing this:
You can choose the outline width and colour.
For more details see our software manual.
Text Labeling / Annotations
... inserts text labels into the live & captured image to highlight or to
comment on points of interest. You can activate it by pressing this:
You can also select the font size, font style and colour.
For more details see our software manual.
E-mail / Print / Cloud
(in offline mode, when the recorded Gallery image is selected)
You can select one or multiple images from the Gallery and then activate
"Send via e-mail" or "Print" or "Save in the cloud" for all selected Gallery
items by pressing these buttons:
Treeview
You can select media destination folder by simple double click on the
existing folder. Or you create your own entirely folder structure inside of
GRYPHAX be using "Treeview". Furthermore, you can rename or delete
existing folder. You can open Treeview by pressing arrow button:
Image Analyzing in External Programs
(in offline mode, when the recorded Gallery image is selected)
You can select one or multiple images from the Gallery and activate
external "Image Analysis" by going to "Preferences">"Additional
Services" to transfer and further process the selected Gallery items to an
external image analysis program. Note: The pre-configured external
software will launch with the files as pre-loaded.
For more details see our software manual.
Grid Scaling and Crosshair
... offers an overlay-frame / mask over the current view within the
Image window in order to correctly prepare the alignment of the
specimen. It can also support your Koehler set up. You have a crosshair
in the exact center of the image which can be activated by choosing the
option under "View":
The grid can be easily adjustable by scrolling in the mouse. A check mark
after the item label within the menu indicates that this mode is
activated. Pressing the "z" button switches back to the previous window
layout. Alternatively, you can close this mode via menu. Grid colour can
be changed under preferences. For more details see our sw manual.
Image Comparing / Side by Side mode
... compares two images in a split-screen side-by-side view. During this
mode all tools are disabled. This mode is not available when running a
multi-fluorescence procedure or during any other "REC" mode (single
capture, video or time-lapse). You can either compare two images from
the Gallery (just drag and drop one image over the other) or you can
compare the live image with an image from the Gallery. (drag and drop
the gallery image over the live image). The related images are arranged
side-by-side within the Image window.
You can be in either active Live mode or Offline mode, depending on the
selected image type. For more details see our software manual.
Exposure
... offers a choice of two modes: Automatic or Expert exposure.
Automatic exposure is activated by default.
You can adjust the auto exposure intensity, "Ocular view" value or set
the grey balance using the pipette. Use the „Ocular view" slider to get
on the monitor what you see in the microscope eyepieces.
The expert settings "White point", "Grey balance", "Black point",
"Exposure time", "Gain" and "Ocular view" are described detailed in our
software manual.
Monochrome mode
... enables you to view the current camera live stream in greyscale
images. If this tool is active all recorded media will be saved in greyscale.
For more details see our software manual.
Keyboard Shortcuts
F1 Open help
("Preferences">"General") F2 Start capturing in Capture Mode (single image, time-lapse, video)
F3 Go back to Live mode
("Preferences">"Camera")
F5 Live image freeze
("Preferences">"Storage options")
F11 Switch between full screen and standard mode
("Preferences">"Device Configuration")
DEL Delete selected images from gallery or the selected (marked) from CMD or CTRL + D Open / Close Treeview
("Preferences">"Status bar")
("Preferences">"Style")
Z
G
You can easily take a (single shot) image capture by pressing "REC":
(in live mode)
(in live mode)
(in live mode)
(in live mode)
(in live mode)
(in live mode)
(via context menu "Measure")
switch zoom between "1:1" & "fit to screen" view
Open / Close Grid
CMD or CTRL + A Select all (all thumbnails from the Gallery)
CMD or CTRL + G Open / Close Gallery
CMD or CTRL + T Open / Close Tools
CMD or CTRL + O Open Preferences storage options
CMD or CTRL + P Print selected image(s) from the Gallery
SPACE BAR activates Magnifier or Hand tool
"+" or "-" to zoom IN or OUT while Magnifier is active