THORLABS OTKBFM-CAL User Manual
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THORLABS OTKBFM-CAL User Manual

THORLABS OTKBFM-CAL User Manual

Calibration and measurement module
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OTKBFM-CAL
Calibration and
Measurement Module for
OTKB with OTKBFM
User Guide

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Summary of Contents for THORLABS OTKBFM-CAL

  • Page 1 OTKBFM-CAL Calibration and Measurement Module for OTKB with OTKBFM User Guide...

  • Page 2: Table Of Contents

    7.1.  Shipping List ........................16  7.2.  Specifications ........................ 16  7.3.  Pin Diagrams ......................... 16  Chapter 8  CE compliance  ........................17  Chapter 9  Regulatory ...........................18  9.1.  Waste Treatment is Your Own Responsibility ............18  9.2.  Ecological Background ....................18  Chapter 10  Thorlabs Worldwide Contacts  ..................19 ...

  • Page 3: Chapter 1 Warning Symbol Definitions

    Calibration & Measurement Module for OTKB/OTKBFM Chapter 1: Warning Symbol Definitions Chapter 1 Warning Symbol Definitions Below is a list of warning symbols you may encounter in this manual or on your device. Symbol Description Direct Current Alternating Current Both Direct and Alternating Current Earth Ground Terminal Protective Conductor Terminal Frame or Chassis Terminal...

  • Page 4: Chapter 2 Safety

    Calibration & Measurement Module for OTKB/OTKBFM Chapter 2: Safety Chapter 2 Safety 2.1. Safety Information For the continuing safety of the operators of this equipment and the protection of the equipment itself, the operator should take note of the warnings, cautions, and notes throughout this user guide, and where visible, on the product itself.

  • Page 5: Chapter 3 Introduction

    Calibration & Measurement Module for OTKB/OTKBFM Chapter 3: Introduction Chapter 3 Introduction The capability of optical tweezers to exert measurable forces on micron-scale, dielectric particles offers a unique and valuable tool for studying cell components, such as biological polymers and molecular motors. In many investigations, optical tweezers need to apply precise force to functionalized microspheres that have been attached to molecules of interest.

  • Page 6: Position Calibration

    The data acquired from the QPD detector is given in volts. For quantitative force and position measurements it is necessary to determine the detector responsivity factor. The method used with the OTKBFM-Cal requires a stuck bead to be moved across the location of the trap while recording the detector voltage. Using the sample stage, steps of known size are used which then allow plotting of the position signal in volts versus the position signal in microns.

  • Page 7: Stiffness Calibration

    3.2. Stiffness Calibration The OTKBFM-CAL software determines the trap stiffness using two approaches: the so called PSD Roll-Off method and the equipartition theorem. The first approach is based on the frequency analysis of the thermal fluctuations of a trapped bead with a known damping. The equipartition theorem calibration on the other hand equates the known thermal energy per degree of freedom with the energy associated with the fluctuations of the particle.

  • Page 8: Chapter 4 Setup

    See Figure 3 below for details. 4. Connect the OTKBFM-CAL module via the USB cable to the PC and connect the power supply to the OTKBFM-CAL module. Ensure that the power supply is set to the correct voltage level for your region and connect the power supply to the OTKBFM-CAL control box.
  • Page 9 5. Start the “APT User” software to configure the controller set. The program should show you a window for each of the K-Cube controllers. The OTKBFM-CAL module requires two piezo controllers, two strain gauge controllers and the PSD controller cube.
  • Page 10 Calibration & Measurement Module for OTKB/OTKBFM Chapter 4: Setup 8. After the strain gauge controllers have been set to zero, you can set the piezo controllers to closed loop mode. Select each of the piezo controller Setting windows and adjust the settings as shown in Figure 5. Make sure that the “Persist Settings to Hardware”...
  • Page 11 “All Program” list. Right click on the “NI USB-6212” device and rename it to “OTKB”. See figure 6. Close “NI MAX.” 12. Start the OTKBFM-CAL software. You are now ready to calibrate your system and run force measurements.

  • Page 12: Chapter 5 Operation

    Calibration & Measurement Module for OTKB/OTKBFM Chapter 5: Operation Chapter 5 Operation Before quantitative measurements can be performed with the optical tweezers system, it is necessary to determine the factor to convert detector voltages to distances and to find the trap stiffness. Changing the trapping laser power during this procedure will affect the calibration parameters.
  • Page 13 Calibration & Measurement Module for OTKB/OTKBFM Chapter 5: Operation 4. Start the Stage Oscillation in the OTKBFM-CAL software, use an amplitude appropriate for the bead size, e.g. 3 µm for a 1 µm bead, and set the frequency to 1 Hz.

  • Page 14: Stiffness Calibration

    5.2. Stiffness Calibration The OTKBFM-CAL software provides two stiffness calibration methods, the so-called PSD Roll-Off and Equipartition calibration. The first approach uses the fact that the thermal motion of a spherical bead of known size suspended in water is well characterized. As the laser power is increased, the Brownian motion of the bead is constrained more and more by the increasing trap force restoring the bead to the center of the trap.

  • Page 15: Sample Preparation

    2. The sample solution can be loaded into the channel using a microscopy slide with built-in channel (offered via our optical trapping accessories kit, Thorlabs item number OTKBTK, sold separately), or you can build a simple channel by placing double-sided tape on a standard slide, and adding a cover glass on top.

  • Page 16: Chapter 6 Frequently Asked Questions

    Check that you have changed the default name of the DAQ card through NI MAX software. The default name the USB-6212 card used is “OTKB”. 2. How come I cannot control the NanoMax stage with OTKBFM-CAL software? a. Make sure that all cable connections are correct. Check the connection between calibration module and the K-Cube controllers.
  • Page 17 Check the connections of the cables from the PSD K-Cube to the OTKBFM-CAL module. d. Check the PSD K-Cube is set to “Monitor Mode” as shown in Fig.4 . Use the Thorlabs APT User software to access the panel shown and adjust this setting.

  • Page 18: Chapter 7 Specifications

    Pin Diagrams The following images describe the connections available on the back side of the OTKBFM-CAL unit. Name of the pins is based on the convention used by the manufacturer of the DAQ card used in the unit, which is part number USB-6212 from National Instruments.

  • Page 19: Chapter 8 Ce Compliance

    Calibration & Measurement Module for OTKB/OTKBFM Chapter 8: CE compliance Chapter 8 CE compliance Page 17 Rev C, August 2, 2018...

  • Page 20: Chapter 9 Regulatory

    Waste Treatment is Your Own Responsibility If you do not return an “end of life” unit to Thorlabs, you must hand it to a company specialized in waste recovery. Do not dispose of the unit in a litter bin or at a public waste disposal site.

  • Page 21: Chapter 10 Thorlabs Worldwide Contacts

    Calibration & Measurement Module for OTKB/OTKBFM Chapter 10: Thorlabs Worldwide Contacts Chapter 10 Thorlabs Worldwide Contacts USA, Canada, and South America UK and Ireland Thorlabs, Inc. Thorlabs Ltd. 56 Sparta Avenue 1 Saint Thomas Place Newton, NJ 07860 Ely CB7 4EX...
  • Page 22 www.thorlabs.com...

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