Turner Designs TD-700 Operating Manual
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Turner Designs TD-700 Operating Manual

Laboratory fluorometer
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TD-700
Laboratory
Fluorometer
Operating
Manual
Dated: 5/8/2002
Version 2.0
Part Number 7000-998
TD-700 Laboratory Fluorometer Operating Manual

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  • Page 1 Page 1 TD-700 Laboratory Fluorometer Operating Manual Dated: 5/8/2002 Version 2.0 Part Number 7000-998 TD-700 Laboratory Fluorometer Operating Manual...
  • Page 2 Page 1 TD-700 Laboratory Fluorometer Operating Manual Dated: 5/8/2002 Version 2.0 Part Number 7000-998 TD-700 Laboratory Fluorometer Operating Manual...

  • Page 3: Table Of Contents

    Introduction A. Description B. Inspection and Setup C. General Precautions D. Definition of Symbols Hardware Overview A. TD-700 Quick View Diagram B. TD-700 Controls III. Optical Filter Installation and Removal Lamp Installation and Removal A. The Mercury Vapor Lamp B. The Quartz Halogen Lamp Instrument Parameters (Firmware) A.
  • Page 4 APPENDIX 9 - Maintenance, Warranty, & Service A. Maintenance B. Warranty C. Obtaining Service APPENDIX 10 - Specifications & Features APPENDIX 11 - Principles of Fluorescence APPENDIX 12 - Instructions for Using the Solid Standard TD-700 Laboratory Fluorometer Operating Manual...
  • Page 5 Page 4 NOTES TD-700 Laboratory Fluorometer Operating Manual...

  • Page 6: Introduction

    Direct Concentration mode. The TD-700 can be set up to detect the specific compound you want to measure simply by changing the lamp and optical filters.

  • Page 7: General Precautions

    Do not defeat the lamp interlock switch. UV light may cause permanent damage to your eyes. Definition of Symbols Direct Current. 12 volts D.C. Easily touched higher temperature parts. Caution. Read instruction manual and refer to warning text. TD-700 Laboratory Fluorometer Operating Manual...

  • Page 8: Hardware Overview

    Alignment Mark Alignment Mark Filter Cylinder Sample Adapter Silver Alignment Mark Sample Chamber Display Keypad TD-700 Laboratory Fluorometer TD-700 Filter Cylinder and 25 mm Round Sample 10 mm Square Cuvette 13 mm Sample Adapter Adapter Adapter TD-700 Laboratory Fluorometer Operating Manual...

  • Page 9: Td-700 Controls

    TD-700 Front View 5. Sample Adapters — Adapters are available for various test tube or cuvette sizes. The sizes used with the TD-700 are: 25 mm round, 13 mm round, 10 mm square, or 100µl minicell. 6. Lamp Access Panel — To access the lamp, remove the panel by pulling on the two black quick-release latches on the lamp access panel.

  • Page 10: Iii. Optical Filter Installation And Removal

    Assembly The TD-700 Fluorometer must have two different optical filters to operate correctly: an excitation filter and an emission filter. TD-700 filters are one- inch diameter, round, colored glass or highly-reflective glass. The filters are mounted in the Filter Cylinder using flexible rubber o-rings. The Filter Cylinder is accessed through the sample chamber.
  • Page 11 Position the Filter Cylinder in the sample chamber so that the align- ment mark for the filter set you are using is aligned with the silver alignment mark on the inside rim of the sample chamber. TD-700 Laboratory Fluorometer Operating Manual...

  • Page 12: Iv. Lamp Installation And Removal

    Page 11 IV. Lamp Installation and Removal There are two types of lamps available for the TD-700: the Mercury Vapor and the Quartz Halogen The Mercury Vapor Lamp The low pressure mercury vapor lamp comes in various types depending Quick-release latches on the application of interest.

  • Page 13: The Quartz Halogen Lamp

    5. Turn on the power. Check to ensure the lamp is lit by viewing it through the fan opening. Also make sure the fan is operating. If the fan is not operating, check the connector plug. 6. You must recalibrate if you replace the lamp. TD-700 Laboratory Fluorometer Operating Manual...

  • Page 14: Instrument Parameters (Firmware)

    Page 13 Instrument Parameters (Firmware) Instrument parameters are set through the TD-700 firmware interface. Firmware screens are called up using the keypad. To see how thescreens fit together, see the firmware flowchart on pages 16 & 17. For details about alarms and instrument diagnostics, see Appendix 3, Section A, and Section B.

  • Page 15: Setup Screens

    Page 14 Setup Screen This screen is used to choose the calibration procedure for the TD-700. Simple Mode -Simple, Multi-Optional Raw Fluorescence, Multi-Optional Direct Concentration. This screen will have 1 of 3 looks depending upon how the 1. Mode instrument is currently configured. (See figure to the left).
  • Page 16 Page 15 NOTES TD-700 Laboratory Fluorometer Operating Manual...
  • Page 17 TURNER DESIGNS Power up Screen <ENT> T7-1B 10/96 Countdown from 600 1. Setup XXX (units) 2. Calibration Home Screen <ENT> Setup &Cal Raw Fluor. Direct conc. Simple Mode Mode Mode XXX (units) 1. Mode 1. Mode 1. Mode 3. Units <ENT>...
  • Page 18 Raw Fluor. Direct conc. Simple Mode Mode Mode Insert Typical Insert Typical Max range: XXXX Insert Blank Sample <ENT> Sample <ENT> 1. OK 9. Change and press < ENT> SETTING Sens Set Sample = XXX Max conc: XXX.X Press <0> when Send Factor: XX 1.

  • Page 19: Vi. Calibration Overview

    Page 18 VI. Calibration Overview A. Why Calibrate? The TD-700 calibration procedures set the instrument's sample range, Note: Calibration data will be (concentration range) and sensitivity based on a chosen fluorescent stored even after power to the standard or sample. In addition, calibration in the Direct Concentration instrument is turned off.
  • Page 20 In a multi-point calibration, the instrument generates a calibration curve for superior accuracy. The TD-700 will display the actual concentration of your samples. The display units are user-selectable and chosen in the setup menu.

  • Page 21: Vii. Calibration: Simple Mode

    Wipe the outside of the test tube or cuvette dry, and insert it into the sample adaptor in the sample chamber. Sensitivity Set The TD-700 will now set sensitivity, as indicated by the SENS FACTOR Sens Factor: XX (sensitivity factor), so that the sample you inserted will read 500 (half of maximum) on the HOME screen.
  • Page 22 Page 21 NOTES TD-700 Laboratory Fluorometer Operating Manual...

  • Page 23: Calibration: Multi-Optional Mode - Raw Fluorescence

    (sensitivity factor), based on the final sample value that you accepted. Once the sample is set, the TD-700 asks whether you want to run a blank. If you wish to have blank subtracted, press <1>. If not, press <9> and the...
  • Page 24 See Appendix 2 for value stable XXX details regarding the calibration printout. Errors: After reading the sample, the TD-700 will automatically adjust to the Reading Blank optimal range for sample measurement. If the sample used is too...

  • Page 25: Calibration: Multi-Optional Mode - Direct Concentration

    Press <ENT>. Enter highest conc Std first! 5. When the TD-700 calls for the “HiStd Conc,” press <1> to accept the current value and advance to the next screen. Or press <9> to change Hi Std Conc: XXX.X the value. Key in the actual concentration of the highest concentration 1.

  • Page 26: How Sample Concentrations Will Be Calculated

    Sens Factor: XX 7. If you are using only 1 standard, the TD-700 will prompt you to insert the Blank. If you are using 2 or more standards, the unit will prompt...

  • Page 27: To View The Last Calibration

    Press <ESC> to return to the HOME Screen. To Abort the Calibration <1> - Abort Cal <ESC> - Resume To abort the calibration, press <ESC> at any time during the calibration sequence. Press <1> to abort or <ESC> to resume. TD-700 Laboratory Fluorometer Operating Manual...
  • Page 28 Page 27 NOTES TD-700 Laboratory Fluorometer Operating Manual...

  • Page 29: Reading Samples

    3. Rinse the cuvette 3 times, preferably with the sample you wish to measure. Repeat steps 1 through 3 until all samples are read. Note: Do not leave highly concentrated samples in the instrument for an extended period of time. Unstable readings may result. TD-700 Laboratory Fluorometer Operating Manual...

  • Page 30: Resetting Blank To Zero

    Data Rate (sec) <1> 2 1. Press <D> from the HOME screen when the data stream feature is on. 2. Use the <↔> key to toggle between numbers. TD-700 Laboratory Fluorometer Operating Manual...

  • Page 31: Appendix 1 - Before Calibration Or Reading Samples

    2. Rinse the test tube or cuvette 3 times between samples (preferably with the sample to be read). The TD-700 is very sensitive and even small amounts of material from a previous sample may result in errors. Complete rinsing is especially important if you are using the same test tube or cuvette for samples and blank.

  • Page 32: Temperature Considerations

    0.1 ppm. At dye concentrations below 0.1 ppm, a single-point calibration (one standard and a blank) may be used to calibrate the TD-700. For concentra- tions between 0.1 and 0.5 ppm, use a multi-point calibration with 3 to 5 different standard concentrations.

  • Page 33: Positioning Samples

    Data Quality The TD-700 is only as accurate as the standards that are used to calibrate it. This is why it is important to take care when preparing standards, samples, and blanks. One should follow good laboratory practices when preparing these standards.

  • Page 34: Appendix 2 - The Calibration Printout

    Printout Capability with a Printer or Computer To use this function with a printer: 1. Connect the TD-700 to a serial printer via the RS-232 port on the back of the instrument. If you ordered a printer from Turner Designs, the appropriate serial cable is included.

  • Page 35: Calibration Printout Using The Direct Concentration Calibration Procedure

    Slope %RSD: 3.127 An Explanation of the Printout The first four lines of the printout display the TD-700's current parameters: the mode, the cal procedure, the units, and the instrument sensitivity level. The sensitivity level (a number between 1 and 100) is a measure of the photomultiplier voltage.

  • Page 36: Calibration Printout Using The Simple Mode

    Adj. Fluor. Blank = 0.000 An Explanation of the Printout The first four lines of the printout display the TD-700's current parameters: the mode, the cal procedure, the units, and the instrument sensitivity level. Following this is the calibration stored in the unit’s memory. The calibration stored consists of the raw and adjusted sample and blank value (if appli- cable), expressed in raw fluorescence units.

  • Page 37: Appendix 3 - Alarms & Diagnostics

    Page 36 APPENDIX 3 Alarms and Diagnostics A. Alarm Screen There are alarms built into the TD-700 to warn of an internal instrument malfunction or low power. Table 1. Alarms Alarm Delay Alarm Condition Normal High Voltage (HV Bad) 3 min.
  • Page 38 Page 37 Raw. The “raw” fluorescence signal output from the instrument’s light detector (the photomultiplier tube). This is the output the TD-700 uses (in conjunction with the actual value entered for the standards, and the fluorescence signal for standards and blank) to arrive at the readout on the HOME screen.

  • Page 39: Appendix 4 - Troubleshooting

    Low readings; Sensitivity was not adjusted adequately Section VII not enough during calibration; recalibrate. Or, make Section VIII resolution sure the proper lamp and filters are Section IX being used. Section III Section IV TD-700 Laboratory Fluorometer Operating Manual...

  • Page 40: Appendix 5 - Data Collection

    You can use various communications software programs to import data to your computer. The signal is sent from the TD-700 in ASCII format. Connect the 9-pin connector that extends from the TD-700 to the serial communications port of your computer (COM1 or COM2) using a 9-pin serial cable.

  • Page 41: Appendix 6 - Sample And Cuvette Adapters

    To install/use adapters 1. Open the lid to the sample chamber. 2. Align pin or point arrow on the adapter to the silver dot on the TD-700. 3. Slide the sample adaptor inside the sample chamber and push down until it is fully seated in the chamber.

  • Page 42: Minicell Adapter

    To install 1. Slide minicell adapter into the 10x10 mm cuvette adapter, where a normal cuvette would sit. 2. Insert the borosilicate glass minicell into the adapter. 3. Insert into the TD-700 as described earlier. TD-700 Laboratory Fluorometer Operating Manual...

  • Page 43: Appendix 7 - Glossary

    Discrete Sample Averaging A feature of the TD-700 which allows a sample reading to be averaged over a set period of time, which mini- mizes operator guesswork, temperature effects, and inconsistent sample handling. When <*> is pressed...
  • Page 44 (Max Range) during calibration in the Direct Concentration calibration procedure. Fluorescence The TD-700 Fluorometer measures the concentration of various analytes in samples of interest via fluorescence. A fluorescent molecule has the ability to absorb light at one wavelength and almost instantly emit light at a new and longer wavelength.
  • Page 45 Sens Factor These three terms are synonymous and are used to Sen % indicate the TD-700 fluorometer's sensitivity on a Sensitivity Level scale of 1 to 100. Sens Factor is the term used in the software during calibration, Sens % can be found on the diagnostic screen, and sensitivity level is the term used on the printout.

  • Page 46: Appendix 8 - Error Messages And Notes

    Page 45 APPENDIX 8 Error Messages and Notes During calibration, the TD-700 will display an error message if incorrect values are entered or if the readings may result in an inadequate calibration. Invalid Input This screen will appear when you enter a value that is outside the range for the parameter.

  • Page 47: Error6

    HOME screen, this error may occur. Most likely this error is a result of using the wrong solution to blank with. To remedy, use the correct blank solution. If the error persists, recalibrate. TD-700 Laboratory Fluorometer Operating Manual...

  • Page 48: Appendix 9 - Maintenance, Warranty, & Service

    4. Plug in the unit and turn on the power. Allow it to run for a few hours until completely dry inside. Periodically wipe off the outside of the instrument with a damp cloth. Do not use solvents or abrasive cleaners to clean the TD-700. Warranty Turner Designs warrants the TD-700 Fluorometer and accessories to be...

  • Page 49: Obtaining Service

    To obtain service during the warranty period, the owner shall take the following steps: 1. Write or call the Turner Designs service department and describe as precisely as possible the nature of the problem. 2. Carry out minor adjustments or tests as suggested by the Service Department.

  • Page 50: Appendix 10 - Specifications & Features

    UL-approved polyurethane. Sheet metal back and base. Readout: Direct Concentration or Raw Fluorescence. Environmental Conditions: Indoor use only. Altitude specification: 0- 2000m. Transient Overvoltages according to INSTALLATION CATEGORY II. Pollution Degree 1 and 2 in accordance with IEC 664. TD-700 Laboratory Fluorometer Operating Manual...
  • Page 51 Optional" for the advanced user. Blank: Reads and subtracts Blank. Discrete Sample Averaging: Sample readings can be averaged to improve accuracy (7-second delay; 12-second signal averaging; 5-second display readout). Warranty: One-year warranty. Approvals: CE, TUV, and UL. TD-700 Laboratory Fluorometer Operating Manual...

  • Page 52: Appendix 11 - Principles Of Fluorescence

    Lamp: The lamp or light source provides the light energy that "excites" the compound of interest. The lamp actually provides a broader range of light than that which excites the compound. This broad light range is illustrated by the "many wavelengths of light" shown in Figure 2. TD-700 Laboratory Fluorometer Operating Manual...
  • Page 53 Light Detector: The TD-700 light detector is a photomultiplier tube. The light allowed to pass through the emission filter, (the wavelengths of light specific to the compound) are detected by the photomultiplier tube. The light intensity, which is directly proportional to the compounds' concentration, is then registered as a digital readout.

  • Page 54: Appendix 12 - Instructions For Using The Solid Standard

    7. Remove solid standard and replace any cuvette adaptors that were removed in step 2. 8. Record instrument settings on TD-700 Calibration Record with Solid Standard sheet that has been included for future reference. 9. Proceed with analyzing your samples.
  • Page 55 Page 54 TD-700 Calibration Record with Solid Standard Instr. Calibration Mode Cal Procedure Maximum “L” “H” Date Std Conc(s) Units (Simple or Multi) (Raw or Direct) Range Reading Reading TD-700 Laboratory Fluorometer Operating Manual...

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