Table of Contents
Related Manuals for Gatan K2 Summit
Summary of Contents for Gatan K2 Summit
- Page 1 K2 Summit and K2 Base Direct Detection Camera User’s Guide Model Number 1000, 1000.S, 1000.B and 1000.U Document Part Number: 1000.40004 Revision 01 Gatan, Inc. 5794 W. Las Positas Blvd Tel. +1 925-463-0200 Pleasanton, A 94588 Fax. +1 925-463-0204...
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Page 2: Table Of Contents
K2 Summit Counted and K2 Summit Super-Resolution ..........24 Updating Summit Hardware Background Reference Image Only ......28 Updating Reference Images for K2 Summit Counted and Super-Resolution Modes ..28 Base Gain Reference Collection ................. 28 Key Points to Ensure Optimum Operation ..............28 ... - Page 3 Magnification Correction/Calibration ..................41 Low Magnification ......................42 High Magnification ......................44 Specifications ........................... 46 Compatibility with Other Gatan Cameras and GIF ............47 Addendum 48 Ensuring Validity of Defect Corrections ................. 48 K2 Summit and K2 Base Direct Detection Camera User's Guide...
- Page 4 ........................9 figure 1-2. Overview of the K2 Base and K2 Summit systems ..........10 figure 1-3. Overview of electron detection process in K2 Base and K2 Summit ....12 figure 1-4. K2 digitizer ......................14 figure 1-5. TEC Controller (top) and K2 Summit Processor (bottom) ........15 ...
- Page 5 High-resolution image of sample to be used in the magnification calibration ..44 figure 1-50. Distance between peaks in the calculated diffractogram ........45 figure 1-51. Calibration instructions ..................45 figure 1-52. Calibration settings ....................46 K2 Summit and K2 Base Direct Detection Camera User's Guide...
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Page 6: Preface
Copyright and Trademarks © 2012 Gatan, Inc. The Gatan logo is a registered trademark of Gatan, Inc. K2, K2 Summit, K2 Base, and DigitalMicrograph are trademarks of Gatan, Inc. All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of Gatan, Inc. -
Page 7: Returns
Support Gatan, Inc. provides free technical support via phone, fax, and electronic mail. To reach Gatan technical support, contact the facility nearest you, or send an email to service@gatan.com or info@gatan.com. -
Page 8: Introduction
Regulatory Pamphlet provided with this product. Review this document in full before installing and operating this product. The K2 Summit™ Direct Detection Camera is a new type of camera for TEM. The camera contains a direct detection transmission CMOS detector, which gives the highest resolution images of any electron-imaging sensor available today. -
Page 9: Figure 1-1. Monte Carlo Simulations Of Electron Interaction With Direct And Indirect Camera System
The el ectron counti ing mode of K K2 Summit re eplaces the an nalogue signa l from each p primary electro on with a disc crete count. Th... -
Page 10: K2 Base And K2 Summit Configurations
“Linear” m mode. figure e 1-2. Overv rview of the K K2 Base and d K2 Summit systems The K K2 Summit Dir rect Detection n camera syst tem also inclu udes the came era, the digitiz... - Page 11 /Å2 low dose image to be recorded in 1-2 s (at a magnification equivalent to 1 Å/pixel). To do so the K2 Summit reads out at a rate of 400 full frames/s (5.7 Gigapixels per second!) using a highly parallelized, high-speed architecture able to process image data at 80 Gb/s.
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Page 12: Figure 1-3. Overview Of Electron Detection Process In K2 Base And K2 Summit
K2 Base an nd K2 Summ Super r-Resolution Counting (K K2 Summit on nly): ach primary e lectron’s sign nal cloud is an nalyzed in the e Summit pro ocessor to dete ermine e electron’s la... -
Page 13: Linear Accumulation Setup Mode
CCD camera does. Individual frames can be summed for an effectively unlimited dynamic range. In K2 Base and K2 Summit linear mode charge is collected, integrated during the exposure, and read out to provide the image. Sensor electron charge proportional to the total energy deposited by the incoming primary electron is accumulated. -
Page 14: Getting Started With The K2 Direct Detection System
Launch DigitalMicrograph (DM). Click the setting button (hammer/wrench) (#1, Figure 1-7, page 16) in the K2 Direct Detection palette. Click the System ON button. Wait for the system to finish turning on. K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 15: Power Off Sequence For K2 Base
1-5. Controller (to op) and K2 S Summit Proc cessor (botto urn on the K2 2 digitizer (blu ue K2 box nea ar the camera a and TEM). K2 Summit and K2 Base Direct Detecti ion Camera U User's Guide... -
Page 16: Figure 1-6. K2 Digitizer
(#3). em ON figure e 1-7. Digita alMicrograph h interface d during startup p of K2 Sum mmit system K2 Summit and K2 Base Direct Detecti ion Camera U User's Guide... -
Page 17: Power Off Sequence For K2 Summit
DONE message in digitizer log window). figure 1-8. DigitalMicrograph interface during shutdown of K2 Summit system Close DM. Turn off K2 digitizer (blue box). Turn off K2 processing rack. Turn off the PC if desired. K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 18: Setting Temperature
This can be done as often as every evening or less frequently (say once a week) during extended periods of data collection. K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 19: K2 Summit Imaging Modes
When the Summit camera version is selected, the three modes available from the K2 Direct Detection palette are Linear, Counted, and Super-Resolution. Linear mode is the way that the K2 Summit camera operates in charge integration mode, while Counted and Super-Resolution are highly processed modes where individual electrons are detected. -
Page 20: Updating Reference Images
Reference must be re-acquired or uploaded from a saved image on disk after power cycling. DigitalMicrograph detects when this has happened and prompts you to upload or re-acquire the references. K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 21: K2 Summit Reference Images
In the next step, you have the option to choose gain reference collection parameters. You may see this warning: figure 1-15. Suggested gain reference exposure factor Click OK to proceed to the setup window. K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 22: Figure 1-16. Settings For K2 Summit Linear Mode Gain Reference
1-16. Settings for K2 Summit linear mode gain reference If you click the Expert Mode box, you have the option to restore default values or select and save your own values for linear mode gain reference collection (if you want a higher quality gain reference or your normal operating conditions are markedly different for instance). -
Page 23: Figure 1-18. Gain Reference Exposure Factor Recommendations
When you adjust the beam and select Done, the script calculates input values for DigitalMicrograph’s gain reference collection routine and fill them in. Do not change these values, and click OK. K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 24: K2 Summit Counted And K2 Summit Super-Resolution
1-22. Linear mode gain reference success message K2 Summit Counted and K2 Summit Super-Resolution Next, if working in K2 Summit mode you are asked if you want to collect the Super- Resolution references (which are also used for counted images). Preparing a gain reference for K2 Super-Resolution mode is optional if it has been completed recently. -
Page 25: Figure 1-24. Hardware Dark Reference Option
Counting modes as the counted reference image is derived from the super-resolution image. The dose rates in the dialog box, multiplied by four, correspond to the per-pixel recommended dose rate for reference images. K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 26: Figure 1-25. Settings For K2 Summit Linear Mode Gain Reference
10 from th he previous s tep. You may y need to chan nge spot size a few steps fo for instance. K2 Summit and K2 Base Direct Detecti ion Camera U User's Guide... -
Page 27: Figure 1-27. Beam Adjustment Reminder
. When it is d done, the refer rences are sav ved. figure e 1-30. Linea ar mode gain n reference s success mes ssage K2 Summit and K2 Base Direct Detecti ion Camera U User's Guide... -
Page 28: Updating Summit Hardware Background Reference Image Only
Base Gain Reference Collection Follow the instructions for K2 Base and K2 Summit Linear reference images, above. Key Points to Ensure Optimum Operation Counted and Super-Resolution Dose Rate: Unlike older cameras, for K2 Summit the DOSE RATE (instead of total dose) becomes the first concern in getting good images. -
Page 29: Other Recommendations
The PC requires approximately 7 minutes to boot. The screen may remain blank during this, do not force it to power down or restart. Do not quit and immediately restart DM. DM requires a few seconds to save data when closed. K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 30: Operation Of The K2 Direct Detection System
The dose recommended for high quality imaging is 10e /pixel/second (8-9 counts/pixel/sec.). In Super-Resolution mode, this dose rate corresponds to 2.5 electrons per Super-Resolution pixel per second. (~2 counts/Super-Resolution pixel/second) K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 31: Dose-Rate Monitor
This setting a applies to all acquisition m modes. figure e 1-35. Pre-D Dose Delay s setting K2 Summit and K2 Base Direct Detecti ion Camera U User's Guide... -
Page 32: Defect Correction
For a description of how to monitor changes in the defect corrections as a method to decide how frequently to prepare a new set of reference image, see Addendum. K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 33: Binning And Subareas
These frames can be viewed independently and used for different options such as frame alignment and drift correction or in some cases advanced usages such local drift correction within regions of a single frame. K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 34: Setup
Dose Fra actionation Se etup palette by y clicking the set tup icon on th he right of the e K2 Dose Fra actionation pa alette. K2 Summit and K2 Base Direct Detecti ion Camera U User's Guide... -
Page 35: Shutter Configuration
When the K2 shutter is in use and active, the beam is not present on the TEM screen. The override signal from the TEM provides an interlock signal to disable the shutter when the K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 36: Shutter Connections And Signal Configuration
Exposure tim mes are given for typical si gnal-particle and tomograp phy applicati ons. When K2 Summit e exposure time es exceed 10s s, K2 Base mo ode with an e exposure time e less than 1s is re ecommended... -
Page 37: Switching Between Base And Summit Modes
To switch to Summit mode (from Base mode): Open the K2 toolbox dialog. Press the Switch to Summit button. Follow onscreen instructions. K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 38: Care Of Detector
The camera cools down to +20°C after the annealing cycle is complete. DigitalMicrograph MUST CONTINUE RUNNING during the anneal cycle. WARNING: Auto Retraction Delay setup (must be longer than longest intended image) K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 39: Insertion Indicators
Camera a Inserted box is checked in th he Camera Vi iew pallet. figure e 1-42. Gatan n logo, and C Camera Inse erted setting Dynam mic Sen sor Prot tection (DSP) O... -
Page 40: Software
Cleaning: Do not attempt to clean the CMOS sensor yourself as damage can result. If the surface of the CMOS sensor becomes dirty or scratched, contact Gatan for service. Sensor replacement: Recommended to only be done by a qualified Gatan service engineer. -
Page 41: Overview: Control Of K2 Camera Within Dm
TEM M is establish hed, the magni fication is rea ad automatica ally. Otherwis se, make sure e DigitalMicro ograph is set t to prompt K2 Summit and K2 Base Direct Detecti ion Camera U User's Guide... -
Page 42: Low Magnification
= 10 x 0.463µm) in the Calibration window and select the units. Select the distance marked in the previous figure to perform the magnification calibration. K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 43: Figure 1-46. Calibration Settings
The calibration can be checked on the calibration table containing pairs of value, the nominal microscope magnification and the calibrated value. To view the magnification table, select Microscope > Calibrations. figure 1-48. Microscope Calibration dialog K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 44: High Magnification
High Magnification Record a lattice image of the crystalline sample. figure 1-49. High-resolution image of sample to be used in the magnification calibration Select Microscope > Calibrate image from Diffractogram. K2 Summit and K2 Base Direct Detection Camera User's Guide... -
Page 45: Figure 1-50. Distance Between Peaks In The Calculated Diffractogram
OK., then e enter the know wn spacing be etween eaks in the ma agnification c alibration in t the Calibratio on settings wi indow. K2 Summit and K2 Base Direct Detecti ion Camera U User's Guide... -
Page 46: Specifications
Nyqui > 0 0.50 at 0.5 of p physical Nyquis > 0 0.06 at 1.25 of physical Nyqu uist Software atan Microscop py Suite includ ding DigitalMic crograph K2 Summit and K2 Base Direct Detecti ion Camera U User's Guide... -
Page 47: Compatibility With Other Gatan Cameras And Gif
The K2 camera is retractable and is mounted below the TEM, in a standard Gatan camera housing, and allowing simultaneous fitting of other cameras, Gatan Imaging Filters (GIFs) or electron energy loss spectrometers, to cover the entire range of imaging requirements. -
Page 48: Addendum
This script can be used to confirm that the gain reference exposure factor has been set high enough (see the section on K2 Summit Counted and Super-resolution gain reference acquisition, page 24) and also to indicate when a fresh hardware dark reference needs to be acquired (page 28). - Page 49 = 128 image rednpssum = realimage("",4,ratsize,ratsize) image rednpsdiff = realimage("",4,ratsize,ratsize) getsize(npssum,width,height) rednpssum[trunc(ratsize*icol/width),trunc(ratsize*irow/height)]+=npssum rednpsdiff[trunc(ratsize*icol/width),trunc(ratsize*irow/height)]+=npsdiff image fpnrat = rednpssum/rednpsdiff - 1 fpnrat[ratsize/2-1,ratsize/2-1,ratsize/2+1,ratsize/2+1]=0 result("Average fixed pattern ratio = "+100*average(fpnrat)+"%\n") Showimage(fpnrat) K2 Summit and K2 Base Direct Detection Camera User's Guide...