HARVARD CMA 7 User Manual

CMA 7 55 kDa Microdialysis Prob e User's Man ual

Instructions for CMA 7 55 kDa Microdialysis Probe

1. Fill a microsyringe with perfusion fluid and mount it in the CMA Syringe Pump. The Perfusion Fluid must be clean, at
room temperature and preferably degassed.
2. Run the pump to make sure that liquid leaves the tip of the syringe cannula.
3. Connect the desired length of tubing to the inlet and outlet of the probe. Short cannula = inlet, long cannula = outlet.
Tubing Adaptors and FEP Tubing should be used for all connections. To facilitate the handling of Tubing Adaptors,
they should be pre-soaked in ethanol for a minimum of 10 minutes.
4. Mount the microdialysis probe CMA
vial filled with perfusion fluid.
5. Connect the inlet tubing of the microdialysis probe to the syringe cannula, by sliding the Tubing Adapter over the cannula.
Wait for 10 min. The Tubing Adapter must be dry before flushing.
6. Flush the probe with 8-10 µL/min in the Perfusion Fluid for 4-5 min to wash out air. While flushing, "tap" with a scissor on
the probe clip (not the probe) to remove air bubbles. The vibrations from the probe clip will in most cases remove the air
bubble. If possible, check for air bubbles under a stereomicroscope. If the air bubble is not gone, the flushing and "tap-
ping" must be repeated. The membrane is light blue when wetted, air bubbles occur as whiter spots. When flushing the
membrane it may appear to be "sweating" which is due to ultrafiltration of fluid through the membrane.
7. Set the pump to the required perfusion flow (usually 1-5 µL/min) and check for leaks.
The microdialysis probe is now ready for use.
8. When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL
INFORMATION). This causes a delay that must be calculated when using low perfusion rates and short sampling times.
9. After the experiment, put the microdialysis probe in a vial filled with deionized water. Perfuse with deionized water to
prevent salt crystal formation. The probe can be stored in deionized water.

TECHNICAL INFORMATION

Membrane
Material
Molecular Cut-Off
Outer Diameter
Length
Probe Shaft
Material
Diameter
Length
Internal Volume
Inlet Volume
Outlet Volume
200 mm Inlet Tubing (blue)
200 mm Outlet Tubing (transparent)
& Probe Clip on the CMA/130 in vivo Stand. Put the microdialysis probe in a
7 8
Polyethersulfone (PES)
Daltons
55,000
0.2 mm
6
1 and 2 mm
Stainless-steel
0. mm
40
7 mm
µL
0.06
0.3 µL
µL
3.5
3.5 µL