Antec DECADE Elite User Manual page 117

CHAPTER 12
Noisy baseline
Possible cause
Salt bridge in REF not satu-
rated
Air bubble in REF or in cell
Slow temperature fluctua-
tions
Fouled WE
Leaking REF or cell
Decreased sensitivity (low S/N ratio)
Possible cause
Fouled WE by dirty sam-
ples
Cell potential too low
Contaminated buffer (high
I
Saturation of output
Possible cause
Damaged REF
Damaged WE
Cell incorrectly connected
Cell potential too high
Base line oscillations
Possible cause
Malfunctioning pump (regu-
lar pattern)
Over-tightened cell bolts
Air bubbles in cell or REF
Temperature oscillations
Contaminated buffer (high
I
Fouled WE
Fe
Troubleshooting
)
cell
)
cell
in buffer
++
Remedy
Refill with saturated KCl, add wetted KCl
crystals
Remove air bubble, continuously degas the
mobile phase
Isolate detector cell, set oven temperature
Clean WE
Tighten connections with care
Remedy
Clean WE, if possible: dilute samples
Optimise potential
Replace buffer, do not recycle the buffer
Remedy
Check with spare REF, replace if necessary
Replace cell block
Check connections (REF: black, WE: red,
AUX: blue)
Optimise cell potential
Remedy
Check pump (seals, valves)
Adjust cell bolts, check pump pressure
Maintenance REF
Set oven temperature
Replace buffer, do not recycle the buffer
Clean WE
Add EDTA, passivate metal parts with HNO
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