ASTEC AD-3100 Operation Manual
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ASTEC AD-3100 Operation Manual

Drawer type incubator
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Drawer Type Incubator
AD-3100
Operation Manual
ATTENTION
In order for you to use the product properly, please read this
instruction manual thoroughly before use and retain it for future
reference. Also, when using the product, keep this manual where
you can retrieve it easily.
Please note that the warranty we provide only covers the product
itself. We cannot compensate for any damage and/or loss to the
contents incurred by the malfunction of the product.

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Summary of Contents for ASTEC AD-3100

  • Page 1 Drawer Type Incubator AD-3100 Operation Manual ATTENTION In order for you to use the product properly, please read this instruction manual thoroughly before use and retain it for future reference. Also, when using the product, keep this manual where you can retrieve it easily.

  • Page 2: Table Of Contents

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. Index of Contents Index of Contexts Index of Contents………………………………………………………………………...2 Index of Contexts 1. Safety Precautions ..................... 3 2. Warranty and Liability ..................7 Index of Contexts 3. Important Notice ....................8 4.

  • Page 3: Safety Precautions

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. Thank you very much for purchasing ASTEC Drawer type incubator AD-3100. In order for you to use this instrument properly and safely, please read this instruction manual thoroughly prior to use and retain it for your future reference.
  • Page 4 AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. WARNING Do not use this instrument outdoors. It could cause an electrical short or a lethal shock. Ask the manufacturer or your sales agent for the setting up. This will prevent negative events such as gas leaks, water leaks or any other unexpected problems caused by mishandling.
  • Page 5 AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. Toxic, pathogenic and radioactive substances should be kept away from the instrument. If this instrument is to be used with toxic, pathogenic, radioactive, and/or other harmful specimens, be sure to conduct such procedures in an isolated facility in compliance with the safety regulations.
  • Page 6 AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. Pedestal, bench, desk or table where the instrument will be set up MUST be solid and leveled even to prevent leakage of the water. Power off and unplug the power cable when moving the instrument to different location.

  • Page 7: Index Of Contexts 2. Warranty And Liability

    We warrant the product you have purchased for two calendar year after the date of purchase. In case of any malfunctions caused by the manufacturer during this period of time, ASTEC will be responsible for repair or replacement of failed parts or even the instrument itself as circumstance allows for free of charge.

  • Page 8: Important Notice

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 3. Important Notice (Read this section to the end prior to use for your own safety) AD-3100 requires separate CO and N cylinders. Siphon type cylinders are not available.

  • Page 9: Index Of Contexts 4. Warning Labels/Bio Hazard/Copy Rights

    4. Warning Labels/Bio Hazard/Copy Rights Warning Labels AD-3100 has an intrinsic danger due to the structure designed for the purpose. For the safe use of the instrument, the following mark is labeled on the instrument. Please use this instrument under a caution indicated on the labels.

  • Page 10: Specifications/ Accessories

    AC220-240V 2.5A(Max.) 50/60Hz *Without adjustors NOTE: Be sure to maintain the room temperature between 24 – 28°C Accessories List of standard accessories associated with AD-3100 needs to be checked once unpacked. Dish Plate Plastic Gas Tube(3m) with a filter Warranty Card...

  • Page 11: Index Of Contexts 6. Dimensions

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 6. Dimensions (mm)

  • Page 12: Parts Description

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 7. Parts Description (12) (10) (11) Hand-Held Chamber (16) (13) (14) (15) (1) Incubation Drawer Chamber (10) Circuit Breaker (2) Incubation Drawer Chamber (11) Power Switch (3) Incubation Drawer Chamber...

  • Page 13: Index Of Contexts 8. Description Of Control Panel

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 8. Description of Control Panel (12) (10) (17) (18) (16) (15) (13) (14) (11) (1) Temperature Display Displays Temperature and setting value for Temperature (2) CO Display Displays CO...

  • Page 14: Prior To Installation

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 9. Prior to Installation  The place (bench, table or stand) where the incubator will be set up MUST be steady, firm and leveled. Make sure the surface supports the weight of the incubator in operational conditions.

  • Page 15: Index Of Contexts 10. Initial Cleaning And Disinfecting

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 10. Initial Cleaning and Disinfecting Initial Cleaning and Disinfecting Cleaning and disinfecting the incubator before its use are critical steps. The initial process and subsequent maintenance should be done properly and following the steps included in this manual.

  • Page 16: Humidity For The Incubation Chamber

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 11. Humidity for the Incubation Chamber Filling the Humidity Slot Water in the humidity water slot should be distilled water and supplied in the slot with approximately 50ml of the water. Humidity water, because it is stagnant, can be common source of contamination within the incubator.

  • Page 17: Index Of Contexts 12. Connecting Gas

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 12. Connecting Gas Connecting CO Gas Supply The Gas port is located on the back of the incubator as shown in the figure below. Connect plastic tubing (supplied with the incubator) and firmly tie them up with cable ties or clamps. The other end of the tubing needs to be connected to gas regulator of gas supply tank or gas manifold.

  • Page 18: Main Power

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 13. Main Power Main power switch is located in the back of the unit as shown in the figure below. Make sure that the AC Power cable is plugged into the power outlet assigned individually for the incubator, then, turn on the unit.

  • Page 19: Index Of Contexts 14. Gas Stop Mode

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 14. Gas Stop Mode Gas-Stop Mode The Gas Stop Mode is recommended to use for setting up the incubator at the initial start-up, or setting it up again after a complete cleaning and disinfection process, or after a long-time unused period.

  • Page 20: Daily Checkup (Gas Concentration)

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 15. Daily Checkup Gas (CO ) Concentration Measurement <Before the measurement> ＊Please be sure to use a calibrated Analyzer with circulating function. ＊Please make sure that supply gas pressures are 0.03Mpa(0.3 bar / 4.35 psi) for CO and 0.05 Mpa...

  • Page 21: Index Of Contexts 16. Gas Circulation / Automatic Calibration

    CO and O chamber is stopped. Automatic Calibrations It is available for AD-3100 to perform calibrations on CO and O sensors once in every week automatically. Only O Span Calibration is set in factory setting. During the calibration of CO sensor, display shows “CAL”...

  • Page 22: Operation Procedure

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 17. Operation Procedure Basic Settings Every operation and parameter can be changed by using <SET> key on the front control panel. Using <UP> and <Down> cursors allows changing the value for the settings or changing the parameters. After changing the value or parameter, and/or if the operation is left unchanged about 7 seconds, it goes back to the normal display, which indicates the current condition of the incubator.

  • Page 23: Index Of Contexts 18. Adjustment And Calibration Menu

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 18. Adjustment and Calibration Menu Adjustment and Calibration Adjustment / Calibration Mode is utilized for modifications to Temperature and CO concentrations by changing factory pre-set parameters as needed. The modifications should be made accordingly to adjust to the environment in which the incubator is used.
  • Page 24 AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. ZERO Adjustment When measured O concentration is different from the O display concentration and it needs to be adjusted, calculate the difference between these two values (A) and change the current value in A-6 by the same amount using <UP>...

  • Page 25: Flow Chart Of Adjustment And Calibration

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 19. Flow Chart of Adjustment and Calibration Normal Display 『UP』+『DOWN』+『SET』 [SILENCE] [SILENCE] [SILENCE] [SILENCE] To ST5 Press Press Press Press ～b-c ～C-A ～ Chamber Temp Do Not Change Adjustment...

  • Page 26: Index Of Contexts 20. Temperature Calibration

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 20. Temperature Calibration Chamber Temperature Adjustment 1. Prepare a calibrated thermometer and attach the sensor at the center of the dish plate surface where indicated as “CAL Point” (Figure A-1). An electronic thermometer with thin lead is recommended.

  • Page 27: Co And O Adjustment/Calibration Procedure

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 21. CO and O Adjustment/Calibration Procedure If the displayed CO and/or O concentration differs from the concentration measured with a calibrated analyzer more or less than the tolerance, adjust the concentration on the display of the incubator to the actual concentration measured with the analyzer according to the Procedure below .

  • Page 28: Index Of Contexts 22. Power Monitoring Relay Unit

    Power Monitoring System connecting an external device or system and output ports located at back side of Cube (AD-3100) is available. Output Terminals A connector shown below is mounted on the back side of Cube (AD-3100) indicated “POWER MONITOR” 3: Unconnected...

  • Page 29: Alarms

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 23. Alarms Alarms Overtemp Alarm OVERTEMP. Alarm activates when the temperature inside the chamber reaches the temperature set on OVERTEMP setting. The alarm triggers a beeping sound that can be shut off by pressing the <SILENCE>...

  • Page 30: Index Of Contexts 24. Troubleshootings

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 24. Troubleshootings Problem Probable Causes: Unit does not power on. IS THE POWER CABLE SECURELY PLUGGED INTO AN ELECTRIC OUTLET?  Make sure that the incubator is securely plugged in.
  • Page 31 AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. Temperature inside the IS THE ROOM TEMPERATURE TOO HIGH?  For the temperature control to work accurately, the room temperature incubation chamber does not needs to be in the range of 24 – 28°C. If the room temperature is reach the target temperature (con’t).
  • Page 32 AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. alarms are activated Is the gas line tubing connected to the correct gas inlet port on the (con’t). incubator?  Be sure that the gas line tube is connected to the correct gas inlet port on the incubator.

  • Page 33: Gas Pressure Regulator

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 25. Gas Pressure Regulator General information Gas Pressure Regulator adjusts high-pressure gas to low pressure to release the gas with regular pressured quantity. There is a high-pressure gas inside the regulator. Use the pressure-resistant device and inspect it regularly to keep normal condition.

  • Page 34: Index Of Contexts 26. Precaution For Set Up

    B) The room to install must be as clean as possible (We recommend Clean Room to install and prior check of cleanliness.) C) Do not install Drawer Type Incubator AD-3100 at places where a. The wind blows against the incubator directly or indirectly such as around door, air conditioning equipment and/or clean bench.

  • Page 35: Cleaning And Disinfectant

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 27. Cleaning and Disinfectant Disinfecting the incubator interior The incubator should be cleaned and disinfected with substances that can be removed with proper rinsing. Any toxic substance or its residue could be biologically toxic which could potentially affect the culture conditions and specimen development.

  • Page 36: Index Of Contexts 28. Precaution To Keep Incubator Clean

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 28. Precaution to keep Incubator clean 【To keep the incubator clean】 The incubator provides a very agreeable environment for microorganisms to proliferate. For this reason, even a small amount of contaminant can cause major contamination in the incubator chamber.

  • Page 37: Wiring Diagram

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 29. Wiring Diagram Wiring Diagram 110V, 115V, 50/60Hz...
  • Page 38 AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 220V, 230V, 240V 50/60Hz...

  • Page 39: Index Of Contexts 30. Maintenance

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 30. Maintenance Descriptions of Gas Sensors and Tubing Heat Protected Box Connector to Heating Element (also one on the other side) Screw (also one on the other side) Back Side Heat Protected Box Replacement Remove 2 screws and gently pull out heat-protected box a few centimeters.
  • Page 40 AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. Descriptions of Gas Control Box Sensor Screws (one on each corner) Sensor Holder IR CO Sensor O Rings Rubber Gasket Screw Gas Control Box Bracket Gas Control Box Sensor Replacement Remove gas control box from the incubator by loosening screws on each side.
  • Page 41 AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. Descriptions of Drawers Temperature Sensor Heating Surface (Beneath the heating surface) Screws Toothed Wheel Magnet Switch (Drawer) Cables Magnet Switch (Incubation Box) Toothed Wheel Remove 4 screws located on the heating surface and remove the aluminum plate.
  • Page 42 AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. Descriptions of Gas supply unit Circulation (OUT) Port Circulation (IN) / N2 Port Knobbed Nut Screws Silicon Gasket Disconnect all the tubes to the Gas supply ports in the back of the unit (please see Descriptions of Gas Sensors and Tubing), and remove the 2 screws holding the gas supply unit.

  • Page 43: Gas Flow Diagram

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. 31. Gas Flow Diagram Descriptions of Gas supply Flow Supply Port Circulation (OUT) Supply Port Circulation (IN)/ Supply Port Circulation (OUT) Supply Port Port Air Filter Flow Volume Valve...

  • Page 44: Factory Settings

    AD-3100 Drawer Type Incubator Operation Manual Rev. 4 ASTEC CO., LTD. Factory Settings S/N: Drawer Drawer Drawer Drawer ASTEC CO., Ltd 4-6-15, Minamizato, Shime, Kasuya, Japan 811-2207 Tel: +81-92-935-5585 Fax: +81-92-936-6613 Email: info@astec-bio.com Website: http://www.astec-bio.com/global/ Ver. 1, issued on 6 February, 2014 Ver.

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