Lumen Dynamics Group X-Cite 120 Series General Maintenance & Troubleshooting

High power, broad-spectrum led solution for fluorescence microscopy

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X-Cite
X-Cite® 120 Series Maintenance

1. Introduction

This guide contains installation, maintenance and troubleshooting tips to help optimize the lifetime and
performance of X-Cite® 120 series and components. This information is intended to supplement the detailed
installation and operating instructions found in the X-Cite® User Manuals.
Topics covered include liquid light guides, lamps, and air vents – the components most likely to affect performance
over time and require maintenance or replacement. Note that the lifetime information on components is for typical
or average conditions. Depending on the imaging application, more frequent replacement may be required—for
example, if a fluorophore's signal is low to begin with, or is excited by a low power portion of the X-Cite® lamp's
output spectrum.

2. Liquid Light Guides

2.1 Proper handling and installation of liquid light guides

Liquid light guides have a typical useful life of 4000 hours of operation when handled properly and installed in a
well maintained X-Cite® 120. The formation of bubbles is one of the most common reasons for a light guide to
degrade prematurely and result in a sudden reduction in illumination intensity. Bubbles can form without warning,
and if they occur within the first 1500 to 2000 hours of use, it is typically due to overheating and/or mechanical
stress to the light guide. Below are some simple tips to avoid overheating and stressing the light guide.
1.
Always fully insert the light guide into the X-Cite® unit (i.e. up to the white line);
this ensures contact with a heat sink to conduct heat away from the light guide.
Guide
®
& Troubleshooting Guide
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Summary of Contents for Lumen Dynamics Group X-Cite 120 Series

  • Page 1 X-Cite Guide ® X-Cite® 120 Series Maintenance & Troubleshooting Guide 1. Introduction This guide contains installation, maintenance and troubleshooting tips to help optimize the lifetime and performance of X-Cite® 120 series and components. This information is intended to supplement the detailed installation and operating instructions found in the X-Cite®...
  • Page 2 X-Cite Guide ® Always allow adequate clearance at the rear of the X-Cite® unit and microscope to prevent excessive bending and/or crushing of the light guide against walls. Minimum bend radius to prevent immediate damage to the LLG is 40mm, however, a bend radius of at least 75mm is recommended for a light guide while “in use”.
  • Page 3 X-Cite Guide ® Look at the quartz at the other end of the LLG Bubble-free: quartz end will appear as a bright, solid circle; you may also be able to see a thin circular outline at the quartz/liquid interface. Bubbles at/near the quartz end: appear as dark spots, as small as 0.5mm in diameter or even as larger more defined spheres.
  • Page 4 X-Cite Guide ® 3. Lamps 3.1 Performance and lifetime The lamp output spectra and typical output levels over lifetime are shown below. X-Cite 120Q Wavelength (nm) Relative Output X-Cite® 120Q vs. HBO 100 1000 1200 1400 1600 1800 2000 Elapsed Time (Hours) Typical Lamp Intensity Over Lifetime for X-Cite®...
  • Page 5 X-Cite Guide ® 3.2 General tips to help maximize lamp lifetime Each time the lamp is ignited, the stress on the lamp effectively decreases lamp life by 5 hours, therefore it is recommended to avoid shutting down the unit during lunch or short breaks or between users, if being used in a multi-user lab After lamp ignition, avoid turning the lamp off until it has run for a minimum of 20 minutes...
  • Page 6 X-Cite Guide ® 4. Air Vents and Filters X-Cite® units have several air vents that are an integral part of the cooling system and overall lamp performance. Proper cooling ensures that lamps operate at optimal temperature and pressure for output power, spectrum, lamp life, light guide life and safety.
  • Page 7 X-Cite Guide ® 4.2 How to check/clean X-Cite® 120XL/Q metal vents When unit is OFF and lamp is COOL, unplug the power from the X-Cite® unit and turn the unit upside down. Visually inspect the metal gratings for lint, dust and other debris. Debris can be removed from the gratings using a small vacuum cleaner with a crevice tool, or blown out with a can of compressed air.

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