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Table of Contents
Troubleshooting
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Summary of Contents for Ovation BioNatural 1160-0020
- Page 1 Operator’s Guide for Multichannel Pipettes L060-0061-001 Rev. F...
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Page 2: Product Description
This manual describes how to use and care for your Ovation multichannel pipette. As you can see from its appearance, it is different from every pipette that you have used before, therefore please read the instructions carefully. -
Page 3: Pipette Components
Recharging Port Power supply connection for battery recharging Power Supply AC adapter for recharging battery (not shown) Captive Screw When loosened, allows access to inside of pipette for battery replacement Ovation BioNatural Pipette – Multichannel Operator’s Guide 1 - 2... -
Page 4: Bionatural Pipetting
The Ovation BioNatural Pipette is the only pipette designed to keep your hand in the neutral position recommended by ergonomics experts. We call this BioNatural™... - Page 5 1. Minimized tension and fatigue 2. Minimized exertion and contact stress 3. Low contact pressure 4. Lowest pressure in carpal tunnel and to median nerve 5. Minimized tension to shoulder and elbow Ovation BioNatural Pipette – Multichannel Operator’s Guide 2 - 2...
- Page 6 – thereby distributing forces and reducing fatigue and effort. Other pipettes do not offer this feature, and on many models pushing on the head would eject the tips. Ovation BioNatural Pipette – Multichannel Operator’s Guide 2 - 3...
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Page 7: Aspirating And Dispensing
Point pipette tips into a suitable waste container and gently press the button. The ejector slide is activated, discarding all tips simultaneously. Ovation BioNatural Pipette – Multichannel Operator’s Guide 2 - 4... -
Page 8: Operating Procedures
OPERATING PROCEDURES Getting Started The general steps to set up and operate the Ovation pipette are: 1. Select a liquid handling function 2. Review and change settings as required 3. Acquire tips 4. Run the liquid handling function according to its protocol 5. - Page 9 S S D D I I L L U U function. V V o o l l The volume to be mixed when using the M M I I X X function. Ovation BioNatural Pipette – Multichannel Operator’s Guide 3 - 2...
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Page 10: Set-Up Of Liquid Handling Functions
Set-up of Liquid Handling Functions Introduction The Ovation multichannel pipette’s keypad is easy to use and provides access to all liquid handling functions and settings. Simply press the buttons on the keypad to step through the various functionss and make changes to settings. When a setting indicator is flashing, this indicates that the setting has been changed from a previously “saved”... - Page 11 Liquid Handling Functions Pipette The Ovation multichannel pipette provides three user-defined pre-sets for routine pipetting at exact volumes with overblow (TC or “to contain” pipetting). Volume and speed are stored for easy recall and use. To set-up and run a P1, P2 or P3 liquid handling function, follow these steps: Example: Pipette 200µL of sample...
- Page 12 Multiple Multiple Dispense is a repetitive pipetting function for dispensing equal volumes. Once a dispense volume and the number of dispenses are established, the Ovation Dispense pipette automatically calculates the total fill volume required. To set-up and run a Multiple Dispense liquid handling function, follow these steps: Example: Dispense a 50µL aliquot, 10 times...
- Page 13 13. To empty the tips of liquid after the final dilution is made, press appears on the display (or to cancel PURGE, press again). Press and release the pipetting trigger to dispense liquid into an appropriate container. Ovation BioNatural Pipette – Multichannel Operator’s Guide 3 - 6...
- Page 14 The pipette is now ready to perform the next Mix function. Z Z E E R R O O 100 . 0 µ L L M M I I X X 5 5 Ovation BioNatural Pipette – Multichannel Operator’s Guide 3 - 7...
- Page 15 The pipette is now ready to perform the next Reverse Pipette function. µL R R e e v v e e r r Ovation BioNatural Pipette – Multichannel Operator’s Guide 3 - 8...
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Page 16: Set-Up Of Pipette Settings
Set-up of Pipette Settings Ovation pipettes use “beep” tones to indicate various actions. A single beep tone Tone indicates the end of a pipetting step. A double beep tone indicates the completion of the (last) dispense cycle when using the multiple dispense or reverse pipette function. -
Page 17: Determining A New Calibration Factor
It is recommended that calibration be verified every six months or on an as-needed basis, whichever is applicable. The Ovation pipette can be easily in-lab calibrated ±10% for optimum performance at your operating conditions. In addition, the calibration factor can be pre-determined and set at the appropriate value for a liquid to be dispensed. - Page 18 Entering a Ovation allows the user to enter a known calibration factor for a specific liquid or Calibration volume, or return to the factory setting. Factor until C C A A L L Repeat Step 2 under “Determining a New Calibration Factor. Press appears on the LCD.
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Page 19: Maintenance And Troubleshooting
MAINTENANCE & TROUBLESHOOTING The Ovation pipette requires minimal maintenance. Always store it in its “standing” position or on the Ovation Pipette Stand (Catalog No. 9058-4003) when not in use. Cleaning Exterior Clean outer surfaces as needed with a soft cloth dampened with warm water. To decontaminate outer surfaces, wipe with a 70% aqueous solution of ethanol or isopropranol. - Page 20 Press down until the end of the tool makes contact with the inside bottom of the nozzle. 4. Tap the nozzle on the counter to remove any loose cellulose material. 5. The nozzle is ready for re-installation. Ovation BioNatural Pipette – Multichannel Operator’s Guide 5 - 2...
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Page 21: General Battery Information
General Battery The Ovation pipette’s battery must be recharged for approximately 90 minutes when Information the pipette is first received, and whenever the battery icon on the LCD is flashing which indicates a low charge. Insert the power plug into recharging port and connect power supply to AC outlet. -
Page 22: Troubleshooting
Probable cause: The remaining battery charge is low Recommended action: Plug AC power supply into Ovation and to an AC source. Begin to use and allow to recharge at a convenient time. Symptom: The pipette does not aspirate or dispense properly... - Page 23 Symptom: A channel(s) leaks during pipetting Probable cause: Non-Ovation tips are being used, tip is not on securely, nozzle is loose, nozzle seal is worn or town, or inner piston or nozzle seal is worn Recommended action: Use Ovation tips. If a single tip leaks, tighten and/or replace that channel’s nozzle. If it continues to leak, inner piston seal may need to be replaced.
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Page 24: Warranty
APPENDIX Warranty VistaLab Technologies, Inc. warrants the Ovation BioNatural Pipette against defects in materials and workmanship for one year from the date of purchase. To register your pipette and activate the warranty, register on the VistaLab Technologies web site at www.vistalab.com. - Page 25 Hazards and Proper use of Ovation pipettes as specified in this manual will ensure safe operation. However, please be aware of the following situations that can damage the device or cause Precautions serious personal injury. WARNING! Connect power supply to a compatible power source. Refer to the voltage configurations shown below.
- Page 26 Power Supply w/4 pipette connections 9060-9006 Base Screw with “O” ring 9057-4006 For the most complete information about specifications, tips, accessories and operating instructions, see our web site – www.vistalab.com Ovation BioNatural Pipette – Multichannel Operator’s Guide B - 1...
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