Table of Contents
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Care and Use Procedures
Minimate
™
TFF Capsule
Table of Contents
Introduction . . . . . . . . . . . . . . . . . . . . . . . .2
Applications . . . . . . . . . . . . . . . . . . . . . . .2
Product Information . . . . . . . . . . . . . . . . .2
Instructions For Use . . . . . . . . . . . . . . . . .3
Installation . . . . . . . . . . . . . . . . . . . . . . . .3
Calibrate Feed Pump . . . . . . . . . . . . . . . .4
Flushing . . . . . . . . . . . . . . . . . . . . . . . . . .4
Sanitization . . . . . . . . . . . . . . . . . . . . . . .5
(Recommended for critical applications)
Normalized Water Permeability (NWP) . . . .5
(Optional)
System Hold-up Volume (Optional) . . . . . .5
Integrity Test (Optional) . . . . . . . . . . . . . . .5
Buffer-Conditioning (Optional) . . . . . . . . . .5
Concentration/Diafiltration . . . . . . . . . . . . .6
Product Recovery . . . . . . . . . . . . . . . . . . .6
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . .6
Storage of the Minimate TFF Capsules . . . .6
Disposal of the Minimate TFF Capsule . . . .6
Appendix A
Normalized Water Permeability . . . . . . . . .7
System Hold-up Volume . . . . . . . . . . . . .10
Minimate TFF Capsule Integrity Test . . . . .10
Flushing and Cleaning After Use . . . . . . .12
Specifications . . . . . . . . . . . . . . . . . . . . .14
Certification . . . . . . . . . . . . . . . . . . . . . .15
Ordering Information . . . . . . . . . . . . . . . .15
Support Literatures . . . . . . . . . . . . . . . . .15
Where to Find More Information . . . . . . .15
PN 88227
7
14
1
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Table of Contents
Summary of Contents for Pall Minimate OAD65C12
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Page 1: Table Of Contents
Care and Use Procedures PN 88227 Minimate ™ TFF Capsule Table of Contents Introduction ......2 Product Recovery ....6 Applications . -
Page 2: Introduction
Product Information The Minimate TFF Capsule comes with threaded luer plugs in each of the 4 ports. A fitting package with the following components is included. Fittings Package Part No. 88216 includes: A Tubing, 3.2 mm (1/8") i.d., 120cm (4 ft) B Male luer-to-hose-barb fittings, 3.2 mm (1/8"), 2 C Female luer-to-hose-barb fittings, 3.2 mm (1/8"), 2 D Tubing clamps, 4... -
Page 3: Instructions For Use
3. Cut a piece of tubing 3.2mm (1/8") i.d., long enough to reach from the feed reservoir, through the pump head to the capsule. NOTE: Pall strongly recommends the use of pressure gauges or transducers connected on both the feed Note: Keep tubing lengths as short as possible to and retentate ports. -
Page 4: Calibrate Feed Pump
Calibrate Feed Pump STEP 2 Note: If the pump does not have a digital read out of flow Adjust pump to deliver a flow rate of about 40 mL/min. rate, it may be useful to calibrate the pump. This makes it Slowly tighten retentate screw clamp to increase easier to set flow rates. -
Page 5: Normalized Water Permeability (Nwp)
A TFF Process Data Sheet is included at the end of See Appendix A for a procedure to determine the minimum the Appendix B for use in recording experimental working volume. conditions. www.pall.com/lab... -
Page 6: Product Recovery
Product Recovery Cleaning Following the concentration/diafiltration process, the If the Minimate ™ TFF Capsule is to be reused, it must be product must be recovered from the system. cleaned after use and properly stored. Recommended cleaning solutions and protocols are given in Appendix A. Product in Concentrate Storage of Minimate TFF Capsules After processing, a significant portion of the product is on... -
Page 7: Normalized Water Permeability
(0) provided there are no restrictions on the filtrate line and that the filtrate line is open to atmosphere. Recirculate the filtrate and retentate streams back to the feed reservoir to minimize the volume of water needed. www.pall.com/lab... - Page 8 Table 2. Table for recording data and calculating NWP Measurement Temperature: ________°C, TCF _______ Feed Retentate Filtrate Filtrate Filtrate Filtrate Water Per- Pressure Pressure Pressure Flow Rate Flux Rate Flux Rate meability LMH/TMP psi/bar psi/bar psi/bar psi/bar mL/min mL/min/cm LMH/TMP @ 20°C) TMP = ( P )/2–...
- Page 9 11.0 LMH/psig x TCF 20 °C where TCF 20 °C = 1.109 (Correcting water permeability for temperature; from 16 °C to 20 °C) Temperature correction factor from Table 3 for 16 °C = 1.109 Normalized Water Permeability = 11.0 x 1.109 = 12.2 LMH/psig www.pall.com/lab...
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Page 10: System Hold-Up Volume
System Hold-up Volume Procedure to determine Minimum Working Volume The system hold-up volume is the total volume contained 1. Add water to the reservoir within the feed/retentate flow path of the TFF system. Most 2. Direct retentate and filtrate lines into feed reservoir. of this volume is recoverable. - Page 11 This protocol describes performing the procedure using a peristaltic pump to generate airflow. For more accurate testing, a regulated, compressed air or nitrogen supply should be used. www.pall.com/lab...
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Page 12: Flushing And Cleaning After Use
PRESSURE-HOLD TEST METHOD (QUALITATIVE) Post Treatment of Minimate TFF Capsule This procedure is a simple, quick method to test the Before introducing the cleaning solution into the membrane integrity of a capsule and system. It involves pressurizing the system, remaining product and contaminants should first be capsule and then watching for a pressure decline. - Page 13 (described on page 5) unusual to find membrane recoveries increase after storage versus values obtained immediately after Water should be wateWFI or at a minimum, 0.2 µm filtered cleaning. DI water. www.pall.com/lab...
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Page 14: Specifications
Appendix B Specifications Materials of Construction Filter media: Omega membrane (m-polyethersulfone) Housing: polypropylene, glass reinforced Screens, housing, housing sealing ring, fittings polypropylene Membrane plate/filtrate channels polyethylene Internal gasket ethylene propylene elastomer Product Dimensions 20cm x 3.8cm x 1.8cm (L x W x H, nominal) 8in x 1.5 in x 0.7 in Effective Filtration Area 50 cm... -
Page 15: Certification
Procedures (PN88227) is supplied on the Minimate TFF Capsule CD-Rom (PN88201) enclosed with the product. Many additional accessories are available for this product, including pumps, reservoirs and connectors. For further assistance, please consult with your local Pall representative or distributor. www.pall.com/lab... - Page 17 1 DV = Remaining Feed Volume at start of diafiltration. Comments Indicate any observations or information considered important to fully describe process or events. To convert milliliters /minute to liters/ hour, multiply by 0.06 To convert square feet to square meters, multiply by 0.093 www.pall.com/lab...
- Page 18 516.625.3610 fax E-mail us at Lab@pall.com © 2003, Pall Corporation. Pall, , Omega, and Minimate are trademarks of Pall Corporation. ® indicates a trademark registered in the USA. is a service mark of Pall Corporation. All rights reserved. *PharMed Masterflex is a registered trademark 6485 of Norton who makes tubing, DuPont makes material.
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