INTELLICYT iQue Screener PLUS Hardware Manual
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INTELLICYT iQue Screener PLUS Hardware Manual

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iQue
Screener PLUS
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hardware manual
December 2016

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Summary of Contents for INTELLICYT iQue Screener PLUS

  • Page 1 iQue Screener PLUS ® hardware manual December 2016...

  • Page 3: Table Of Contents

    IntelliCyt Corporation Software License Agreement . . . . . . . . . . . . . . . . . .
  • Page 4 Connecting iQue Screener PLUS Power and Data Cables . . . . . . . . . . . . . . . .

  • Page 5: Introduction

    The iQue Screener PLUS uses ForeCyt and IntelliCyt Data Manager software which require licensing before use. Statement of Proper Use The iQue Screener PLUS is designed to acquire samples in a multi-well microplate to perform high-throughput flow cytometry. The iQue Screener PLUS is designed for use in a temperature-controlled laboratory environment that is free of conductive contaminants.

  • Page 6: End User Use Agreement For Ique Screening System

    IntelliCyt; or (c) has not been maintained in accordance with the Documentation; or (d) if the Product’s serial number has been removed or defaced; or (e) has been incorporated into a system without IntelliCyt’s review and written approval.
  • Page 7 This indemnification applies only to the Product delivered by IntelliCyt and shall not apply if the Product has been modified by a party other than IntelliCyt, or if the Product has been combined with (or used in connection with) other products and used as a part of an infringing process or method which, but for the combination, would not infringe the intellectual property rights of such third party.

  • Page 8: Intellicyt Corporation Software License Agreement

    1) License Grant: IntelliCyt Corporation (“Licensor”), grants the purchaser (“Licensee”) a non-exclusive, non-transferable license (“License”) to use the Software, and associated manuals and documentation, in conjunction with hardware configurations authorized by IntelliCyt Corporation, at the designated Licensee’s business location until this License is terminated in accordance with the terms and conditions specifically set out in the Agreement.

  • Page 9: Declaration Of Conformity

    Declaration of Conformity iQue Screener PLUS Hardware Manual: Part Number 11811 Rev B, Introduction ®...
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  • Page 11: Using This Manual

    Using This Manual Conventions This guide assumes basic knowledge of computers using Microsoft Windows operating systems and familiarity with windows, menus, commands, buttons, tabs, dialog boxes, and other Microsoft Windows elements. The terminology, conventions, and symbols used in this guide are described next. Terminology Term Definition...

  • Page 12: Text Symbols

    Text Symbols Symbols are used in iQue Screener PLUS documentation to draw attention to important information, such as operating tips and suggestions, as well as the presence of hazards. Symbol Description NOTE A tip, suggestion, or additional information. WARNING Indicates information that is necessary for proper instrument or software operation.

  • Page 13: Accessing Technical Support

    Accessing Technical Support For help with a technical question that is not addressed within the documentation, please contact support@intellicyt .com or IntelliCyt Customer Service at (505) 345-9075 Opt 2. Before contacting us, please be prepared to answer the following questions: •...
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  • Page 15: Safety

    IntelliCyt approved uninterruptable power supply. General Warnings THE iQue SCREENER PLUS IS INTENDED FOR GENERAL LABORATORY AND RESEARCH USE ONLY. IT IS NOT INTENDED FOR IN VITRO DIAGNOSTIC TESTING. READ THE ENTIRE iQue PLUS SCREENER SYSTEM HARDWARE MANUAL BEFORE ATTEMPTING TO OPERATE THE INSTRUMENT.

  • Page 16: Operator Safety

    Operator Safety WARNING: There is a risk of operator injury if: • The covers are not opened and/or closed with care. • All covers and panels are not closed and secured in place prior to and during instrument operation. • Contact is made with moving parts.

  • Page 17: Safety Symbols And Labels

    Safety Symbols and Labels Safety symbols and labels draw attention to potentially dangerous conditions. These symbols, together with text, apply to specific procedures and appear as needed on the instrument and throughout this user guide. Do not remove these labels. Biohazard/Biological Risk: Consider all materials (specimens, reagents, controls, etc.) and the areas these materials come into contact with as being potentially infectious and/or life threatening.

  • Page 18: Biological Handling Precautions

    Biological Handling Precautions Biological samples are potentially dangerous and/or life threatening. Adhere to proper handling procedures for samples and reagents at all times. Depending on the laboratory environment, there could be a risk of biological, chemical, or radiological contamination resulting from contact with samples, sample tubes, sample waste, the waste container, and/or associated tubing.

  • Page 19: Operational Precautions

    Keep cords away from heat, oil, or sharp edges. Damaged cords increase the risk of electric shock. CAUTION: The instrument must be plugged into an IntelliCyt approved uninterruptable power supply (UPS). The UPS must be plugged into a standard, grounded, or earthed mains electrical outlet, conforming to local codes. Non- grounded or non-earthed mains outlet adaptors must not be used.
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  • Page 21: Getting To Know The Ique Screener Plus

    Screener PLUS Overview IntelliCyt’s iQue Screener PLUS is a platform for screening suspension cells, beads, microbes and mixtures. The system consists of a robotic plate rail with an orbital shaker, robotic plate sampler with a contact sensing probe, peristaltic pump, detector, and ForeCyt screening software to design, run, and analyze screening assays.

  • Page 22: Specifications

    PC Operating System Microsoft Windows 7 or Windows 8 64-Bit ForeCyt Enterprise Database with Distributed Clients Options Automation Interface, 21 CFR Part 11 iQue Screener PLUS Hardware Manual: Part Number 11811 Rev B, Getting to Know the iQue Screener PLUS ®...

  • Page 23: Fluorescence Channels

    Fluorescence Channels – BR The iQue Screener PLUS – BR has two lasers and six fluorescence channels. The table below lists these channels, their associated filter, and gives a few example dyes for each. Channel Position Filter Example Dyes 530/30...
  • Page 24 Fluorescence Channels - VBR The iQue Screener PLUS–VBR has three lasers and thirteen fluorescence channels. The table below lists these channels, their associated filter, and gives a few example dyes for each. Channel Position Filter Example Dyes 445/45 Pacific Blue, BV421, DAPI...
  • Page 25 Fluorescence Channels - VYB The iQue Screener PLUS–VYB has three lasers and thirteen fluorescence channels. The table below lists these channels, their associated filter, and gives a few example dyes for each. Channel Position Filter Example Dyes 445/45 Pacific Blue, BV421, DAPI...

  • Page 26: Front View

    Front View Sampling Area Shaker Status Light Light Switch iQue Screener PLUS Hardware Manual: Part Number 11811 Rev B, Getting to Know the iQue Screener PLUS ®...

  • Page 27: Status Light Detail

    Ready. The iQue Screener PLUS will continue to run if it encounters a warning. For example, a “Waste Bottle is close to full” warning will not cause the current run to stop. However, if the iQue Screener Plus encounters an error, such as a “Sheath is empty”...

  • Page 28: Shaker Detail

    Pins (2 of 4) Shaker Body Sampling Area Detail Shaker Cable Guide Microcentrifuge Tube Holder Plate Rail Fluid Cartridge (1 of 4) iQue Screener PLUS Hardware Manual: Part Number 11811 Rev B, Getting to Know the iQue Screener PLUS ®...

  • Page 29: Sampler Detail

    Probe Guide Inner Probe Pump Detail Peristaltic Pump Housing Tubing Clamp Pump Rollers (12) Release Trigger Sample Tubing Optics Cover Tubing Port iQue Screener PLUS Hardware Manual: Part Number 11811 Rev B, Getting to Know the iQue Screener PLUS ®...

  • Page 30: Right Side Panel Detail

    PLUS One Port Waste Port PLUS Two Port Bottle Station Sheath Bottle PLUS One Bottle PLUS Two Bottle Waste Bottle Bottle Station iQue Screener PLUS Hardware Manual: Part Number 11811 Rev B, Getting to Know the iQue Screener PLUS ®...

  • Page 31: Fluidics Harness Detail

    Auto-closing Quick Connects Fluid Line Labels Harness Direction Label Back Panel Detail Serial Number Fuse Holder Power Switch Power Socket Data Port iQue Screener PLUS Hardware Manual: Part Number 11811 Rev B, Getting to Know the iQue Screener PLUS ®...

  • Page 32: Front Service Area

    Fluidlink and sheath filter are the only user serviceable parts in this area. Sheath Filter Strap Luer Sample Inlet Port Fitting Fluidlink Connection Nut Fluidlink Connection In-line Sheath Filter iQue Screener PLUS Hardware Manual: Part Number 11811 Rev B, Getting to Know the iQue Screener PLUS ®...

  • Page 33: Optical Bench And Pmt Module Detail

    PMT (1 of 4) Dichroic Mirror (1 of 6) Band Pass Filter (1 of 5) CAUTION There are no user serviceable parts in this area. iQue Screener PLUS Hardware Manual: Part Number 11811 Rev B, Getting to Know the iQue Screener PLUS ®...
  • Page 34 PMT (1 of 6) Dichroic Mirror (1 of 6) Band Pass Filter (1 of 7) CAUTION There are no user serviceable parts in this area. iQue Screener PLUS Hardware Manual: Part Number 11811 Rev B, Getting to Know the iQue Screener PLUS ®...
  • Page 35 PMT (1 of 6) Dichroic Mirror (1 of 6) Band Pass Filter (1 of 7) CAUTION There are no user serviceable parts in this area. iQue Screener PLUS Hardware Manual: Part Number 11811 Rev B, Getting to Know the iQue Screener PLUS ®...
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  • Page 37: Operation

    Operation Articles • Daily Startup • Daily QC • Running a Plate • Daily Shutdown • iQue Screener PLUS Bottle Fluids • iQue Screener PLUS Deck Fluids iQue Screener PLUS Hardware Manual: Part Number 11811 Rev B, Operation ®...
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  • Page 39: Daily Startup

    Daily Startup Overview This procedure explains how to perform a proper daily startup of the iQue Screener PLUS. Steps Fill the Sheath, PLUS ONE, and PLUS TWO bottles and empty the Waste bottle. Refer to the iQue Screener PLUS Bottle Fluids chapter for detailed instructions.
  • Page 40 If ForeCyt is not started, use the desktop shortcut to launch ForeCyt. When prompted to power on the detector, click Yes. Make sure that the fluid link and peristaltic pump tubing clamp are properly installed as shown in the iQue Screener PLUS Startup Wizard and then click Start.

  • Page 41: Daily Qc

    Running daily quality control is highly recommended. A daily QC check not only ensures that system performance is within specification, but is also a useful diagnostic tool if issues arise. IntelliCyt makes the QC process easy by providing reagents and pre-configured analysis templates. Daily QC should take less than 5 minutes to complete.
  • Page 42 Standard Cleaning Procedures). If the test still fails, contact IntelliCyt Technical Support. The specific definition of each test can be observed by selecting the metric in the list and looking at its calculation expression. For example, the “TEST: Bead Count” metric passes if the test recorded more than 30,000 events.

  • Page 43: Running A Plate

    This example experiment was run using a simple suspension cell line. Final cell concentration was 1,000 cells per µL. 30 µL were put in each well of a V-bottom 96-well plate. When preparing your sample, be sure to adhere to IntelliCyt’s best practices for assay development and optimization which is covered in detail elsewhere. Please contact support@intellicyt .com...
  • Page 44 Step 2 – Create a New Experiment • Click the New Experiment button on the ForeCyt toolbar. ® • In the New Experiment dialog, choose Blank Experiment, Plate Type = 96 Well, give your experiment a name and click Save. Your new, blank, 96 well experiment will be created and opened.
  • Page 45 IntelliCyt recommends collecting 1000–2000 events of interest per well for statistical relevance in most assays. IntelliCyt also recommends the Standard (29 RPM) speed for most assays. At this speed, the instrument delivers between 1.5 and 2 µL per second. Taking your cell concentration and the percentage of cells that qualify as events of interest (for example, CD4+ normal singlets), you can calculate Sip Time.
  • Page 46 Step 4 – Run the Plate Open the grippers on the deck by pulling back the gripper release lever and place your plate on the deck. In the Controller Console window, click Run. As the plate runs, you can monitor the run progress by observing the default scatter plot and looking to the Controller Console for additional plate statistics.
  • Page 47 Step 5 – Perform Well Identification Click on the Well Identification tab and click the Calculate Wells button. In the Noise Filter for Well Identification dialog, zoom in, choose a gating tool, and draw a gate around a population that exists in all of your wells. Then click Close. At this point, your wells are calculated.
  • Page 48 This is an example of a very simple data analysis showing some of the basic techniques in ForeCyt. For detailed instructions on ForeCyt data analysis see the video tutorials on the IntelliCyt website. A FSC vs. SSC scatter plot is used to identify normal and dying cells based on their morphology.

  • Page 49: Daily Shutdown

    Daily Shutdown Overview This procedure explains how to perform a proper daily shutdown of the iQue Screener PLUS. Steps Optional Long Clean: At least once a week, and particularly after heavy usage, perform a Detector Long Clean. Click Clean in the Controller Console.
  • Page 50 Follow the instructions in the Shutdown Wizard and click Start to initiate the shutdown. iQue Screener PLUS Hardware Manual: Part Number 11811 Rev A, Operation: Daily Shutdown ®...

  • Page 51: Ique Screener Plus Bottle Fluids

    Screener PLUS Bottle Fluids Overview This article describes the fluids in the iQue Screener PLUS Bottle station, how they are prepared, and how the bottle station liquid level detection works. Bottle Station Bottle Purpose Preparation 1 bottle of IntelliCyt Bacteriostatic Con-...
  • Page 52 The bottle connectors are auto-closing quick-connects. If they are not firmly re-attached, the valve will remain closed, fluid will not be supplied, and the instrument will enter a low pressure error status. iQue Screener PLUS Hardware Manual: Part Number 11811 Rev A, Operation: iQue Screener PLUS Bottle Fluids ®...
  • Page 53 Do not attempt to fill or otherwise touch the bottles while the instrument is running. NOTE For liquid level detection to function properly, the bottles must be placed in their proper color-coded positions. iQue Screener PLUS Hardware Manual: Part Number 11811 Rev A, Operation: iQue Screener PLUS Bottle Fluids ®...
  • Page 54 Controller Console and the system will halt operation. CAUTION The bottle station must be placed at the same level as the instrument otherwise low pressure errors may result. iQue Screener PLUS Hardware Manual: Part Number 11811 Rev A, Operation: iQue Screener PLUS Bottle Fluids ®...

  • Page 55: Ique Screener Plus Deck Fluids

    Overview This article describes the fluids found in cartridges or troughs in the iQue Screener PLUS sampling area. In the sampling area of the iQue Screener PLUS there are slots for four quarter module troughs. These can be filled using pre-prepared IntelliCyt fluid cartridges (recommended and shown) or with empty troughs filled with hand mixed solutions.
  • Page 56 QSol Buffer IntelliCyt’s standard Buffer formula of PBS + .1% BSA is tried and true and has worked for millions of samples. However, in an effort to continuously improve the robustness of the iQue Screener PLUS platform, IntelliCyt now offers QSol Buffer which is a higher performance alternative to standard Buffer and which can help prevent clogging and aggregation with the most challenging sample types.
  • Page 57 Dirty fluid troughs are a common source of contamination. Replace troughs as needed and always use fresh fluids. CAUTION Overfilling troughs may cause contact sensor faults and cross-contamination. iQue Screener PLUS Hardware Manual: Part Number 11811 Rev A, Operation: iQue Screener PLUS Deck Fluids ®...
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  • Page 59: Maintenance

    Maintenance Articles • Recommended Maintenance Schedule • Standard Cleaning Procedures • Changing the Probe and Tubing Fluidics Maintenance • • Managing Plate Models iQue ® Screener PLUS Hardware Manual: Part Number 11811 Rev A, Maintenance...
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  • Page 61: Recommended Maintenance Schedule

    Schedule Overview iQue Screener PLUS maintenance involves several procedures that need to be performed regularly. Some of these procedures can be performed more frequently as needed based on usage and sample types. Working with samples that are prone to aggregation may require more frequent cleaning and maintenance.
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  • Page 63: Standard Cleaning Procedures

    Standard Cleaning Procedures Overview The ForeCyt software supports several standard cleaning procedures. This article explains what each one does and when to use it. All of the standard cleaning procedures are accessed by clicking the Clean button and using the Clean Wizard.
  • Page 64 Probe and Tubing Clean This clean runs a sequence of Decon, Cleaner and Water through the probe and tubing using the solutions on the sampling deck. This clean should be used any time where you suspect clogging or contamination in the probe and tubing, for example, when the Daily QC water test returns a higher than normal result.
  • Page 65 Detector Unclog This procedure is used when a clog in the detector is suspected and also as part of regular monthly maintenance. It takes about ten and a half minutes to run. Detector Debubble This procedure is used to clear air bubbles from the detector fluidic lines and bottles. Use if Daily QC shows irregular scatter, after fluidics maintenance and when fluids are added to the bottles.
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  • Page 67: Changing The Probe And Tubing

    Changing the Probe and Tubing Overview As the iQue Screener PLUS runs, the main sample tubing is worn by the rollers of the peristaltic pump. To ensure proper sample collection, IntelliCyt recommends that the probe and tubing assembly be changed after 20 hours of usage.
  • Page 68 Launching the Change Probe and Tubing Wizard The Change Probe and Tubing Wizard will guide you through the process of changing the probe and tubing. To launch the wizard, click DeviceChange Probe and Tubing… This will launch the Change Probe and Tubing Wizard. The Welcome screen will display the total runtime since the tubing was last changed.
  • Page 69 Wizard Steps Remove Probe and Tubing. Prepare Probe and Tubing Assembly. iQue Screener PLUS Hardware Manual: Part Number 11811 Rev A, Maintenance: Changing the Probe and Tubing ®...
  • Page 70 Replace Probe and Tubing. NOTE When routing the tubing in the pump head, take care not to crimp the tubing in the locking key.  Crimped Correct iQue Screener PLUS Hardware Manual: Part Number 11811 Rev A, Maintenance: Changing the Probe and Tubing ®...
  • Page 71 Calibrating Deck Height When the Change Probe and Tubing Wizard completes, the Calibrate Deck Height Wizard will automatically launch. It is important to recalibrate the deck height after a probe and tubing change. When the wizard launches, click Next to continue.
  • Page 72 Cleaning the New Tubing When the Deck Height Calibration Wizard finishes, you will be prompted to clean the probe and tubing. Ensure that the Decon, Cleaner and Water troughs/cartridges in the fluid stations are full and click Yes and then fol- low the Clean Wizard to perform a Probe and Tubing Clean.

  • Page 73: Fluidics Maintenance

    Fluidics Maintenance Overview The iQue Screener PLUS detector has several parts that are designed to be regularly replaced in order to keep the system running in optimal condition. These include various filters as well as the FluidLink. The process of replacing these parts is called “fluidics maintenance.”...
  • Page 74 When the wizard launches, a list of required items is displayed. Make sure you have all the supplies that are needed. Wizard Steps Empty and Clean Fluid Bottles. iQue Screener PLUS Hardware Manual: Part Number 11811 Rev A, Maintenance: Fluidics Maintenance ®...
  • Page 75 Replace Bottle Filters. Replace In-line Sheath Filter. iQue Screener PLUS Hardware Manual: Part Number 11811 Rev A, Maintenance: Fluidics Maintenance ®...
  • Page 76 Remove Probe and Tubing. Replace FluidLink. Take care when threading the new FluidLink into the detector inlet. Make sure NOTE the tubing is flush with the ferrule. Make sure the threads are aligned and the FluidLink is securely finger tightened and do not over-tighten. Do not use a tool to tighten the FluidLink.
  • Page 77 Correct (inner tubing flush with ferrule) Incorrect (inner tubing protruding) Incorrect (inner tubing recessed) Replace Probe and Tubing. iQue Screener PLUS Hardware Manual: Part Number 11811 Rev A, Maintenance: Fluidics Maintenance ®...
  • Page 78 Fluidics Check. Priming the System. iQue ® Screener PLUS Hardware Manual: Part Number 11811 Rev A, Maintenance...

  • Page 79: Managing Plate Models

    Managing Plate Models Overview For different experiments, researchers use different kinds of plates with different geometrics, depths and character- istics: v-bottom vs. round-bottom vs. flat bottom; shallow vs. deep; circular vs. square. This article discusses how to set up and maintain a user-defined library of plate models that can be used when defining an experiment protocol. Understanding Probe Depth Calculation and Plate Model Mapping The formula for calculating probe depth is: Deck Height + Mapped Plate Model Offset (for that well) + Fixed Sampling Offset.
  • Page 80 Plate Model Selection is Part of Protocol Each plate on each experiment has a set of Protocol settings that control how the plate is run. On the Sample section, there is a field for Plate Model. This is where the plate model is selected. By default, the system comes with only two plate models listed: the Generic 96 Well Plate and Generic 384 Well Plate models.
  • Page 81 Adding a New Plate Model Click Device > Manage Plate Models. In the Manage Plate Models screen, click Add and enter the name, manufacturer and type for the new plate model. Click Save. A confirmation prompt will appear for calibrating the plate model. Click Yes. iQue Screener PLUS Hardware Manual: Part Number 11811 Rev A, Maintenance: Managing Plate Models ®...
  • Page 82 This will launch the Map and Calibrate Wizard. Place an example of the plate you are adding in the gripper and click Next. As soon as you click Next, the instrument will map twelve or more depth positions on the plate.
  • Page 83 This will bring you to the optional Set Sample Height step. By default, the sampling offset is .5 mm. This means that the probe tip will go to a location .5 mm above the bottom of the well. This is optimal for most assays.
  • Page 84 This will bring you to the Optional Set Offset Location step. The normal sample position is from the center of the well. When using flat bottom plates, however, the vortexing action of the shaker causes sample to accumulate on the edges of the well so it becomes necessary to sample from the side or corner of the well. The optional Offset Location step makes this possible.
  • Page 85 If you want to configure an offset location, click the Modify button to enable the controls and then use the X and Y arrows to move the probe to an offset position. No XY-Offset: probe samples from center XY-Offset: probe samples from edge of well (96 Well v-bottom plate shown). of the well (96 well flat bottom plate shown).
  • Page 86 Setting the Default Plate Model for each Plate Type When creating a blank experiment (i.e., not from a template) a default plate model for the format (96 or 384 well) is chosen. To customize which plate model is used as the default follow the steps below. Click Device >...

  • Page 87: Manual Control Mode

    Manual Control Mode Overview Manual Control Mode is a feature of the ForeCyt software that allows you to interact with the various instrument ® subcomponents directly. This can be helpful when troubleshooting instrument problems. Accessing Manual Control Mode To access Manual Control Mode click Device > Manual Control Mode. This will bring up the Manual Control Mode screen.
  • Page 88 Test a Shake RPM If you have a large volume in your wells (for example more than 50 µL in a 96-well plate) there is a risk that the default shaker speed will be too fast and the sample will vortex out of the well. To find a shaker speed that is “safe,”...
  • Page 89 Troubleshoot a Clog When the iQue Screener PLUS appears to have a clog it is not always obvious if the clog is in the probe and tubing or in detector. Use Manual Control Mode to isolate these subsystems and troubleshoot.
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  • Page 91: Clog Prevention And Management

    Flow Cell Clog: A much less common clog is one in the flow cell of the detector. These can usually be resolved using Standard Cleaning Procedures mentioned earlier in this section. Please contact IntelliCyt Technical Support at support@intellicyt .com if you suspect a flow cell clog.
  • Page 92 Insufficient or improper buffering is the leading cause of probe and tubing clogs. It is essential to use either IntelliCyt’s original Buffer (PBS + .1% BSA) or IntelliCyt’s new and improved QSol buffer in the Buffer station. Other solutions, such as water or pure PBS, do not properly coat the probe and tubing and are not effective at preventing aggregation and clogging.
  • Page 93 Then click Add… which brings up the Add Rinse button. Set the Rinse Station to Buffer and the time to 1 second and click OK. Repeat these steps two more times to create a 3 second Buffer rinse configured as three 1 second rinses: In this example, we’ve created a 3 second rinse after every 6 wells which will add about 50 seconds to a 96-well plate run.
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  • Page 95: Special Topics

    Special Topics Articles • Moving an iQue Screener PLUS • Connecting iQue Screener PLUS Power and Data Cables • Purge and Prime iQue ® Screener PLUS Hardware Manual: Part Number 11811 Rev B, Special Topics...
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  • Page 97: Moving An Ique Screener Plus

    Moving an iQue Screener PLUS Overview Describes the procedure for safely moving an iQue Screener PLUS from one location to another within the same lab or building. This does not include instructions for packaging the iQue Screener PLUS for shipment. If the need to package and ship an iQue Screener PLUS arises, contact IntelliCyt Technical Support.
  • Page 98 Reconnect the power and data as described in Connecting iQue Screener PLUS Power and Data Cables. Fill all the bottles and troughs and perform Daily Startup Daily iQue ® Screener PLUS Hardware Manual: Part Number 11811 Rev B, Special Topics: Moving an iQue Screener PLUS...

  • Page 99: Connecting Ique Screener Plus Power And Data Cables

    Overview This procedure shows the proper way to connect the iQue Screener PLUS power and data cables. It is used as part of first time startup and also any time that the instrument needs to be physically moved to a new location.
  • Page 100 Check the UPS for warnings or alarms that indicate faulty or inadequate power or a defective UPS batter. Plug the iQue Screener PLUS main power into one of the protected and battery backed up sockets on the UPS as shown in the diagram below.
  • Page 101 110V Power and Data Connection Diagram 220V Power and Data Connection Diagram iQue ® Screener PLUS Hardware Manual: Part Number 11811 Rev B, Special Topics: Connecting Power and Data Cables...
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  • Page 103: Purge And Prime

    Overview Whenever an iQue Screener PLUS is going to be inactive for a long period of time (longer than one month) and whenever it may be placed in an airplane for transport, it should be purged to remove all fluids from the lines. This prevents bacterial growth and protects the fittings and lines from freezing.
  • Page 104 Ensure that you have the required supplies and time to complete the operation as shown in the introduction to the Purge Fluidics Wizard. Wizard Step 1: Flushing System. iQue ® Screener PLUS Hardware Manual: Part Number 11811 Rev B, Special Topics: Purge and Prime...
  • Page 105 Wizard Step 2: Purging System. Wizard Step 3: Purge Pressure Sensor Lines. iQue ® Screener PLUS Hardware Manual: Part Number 11811 Rev B, Special Topics: Purge and Prime...
  • Page 106 Wizard Step 4: Reconnect Pressure Sensor Lines and Power Down. iQue ® Screener PLUS Hardware Manual: Part Number 11811 Rev B, Special Topics: Purge and Prime...
  • Page 107 Priming Once an iQue Screener PLUS has been purged, it must be re-primed before it can be used again. The installation priming process takes approximately 40 minutes and requires an inline sheath filter, a Fluid Link and a Probe and Tubing assembly and an empty 500 mL beaker.
  • Page 108 Wizard Step 1: Initial Prime. Wizard Step 2: Priming the Pressure Sensors. iQue ® Screener PLUS Hardware Manual: Part Number 11811 Rev B, Special Topics: Purge and Prime...
  • Page 109 Wizard Step 3: Reconnect the Pressure Sensor Lines and Install Sheath Filter. Wizard Step 4: Connect the FluidLink, Probe and Tubing. iQue ® Screener PLUS Hardware Manual: Part Number 11811 Rev B, Special Topics: Purge and Prime...
  • Page 110 Wizard Finish – Final Prime. After the system has been primed, click Power on Detector to start the detector (see Daily Startup). iQue ® Screener PLUS Hardware Manual: Part Number 11811 Rev B, Special Topics: Purge and Prime...
  • Page 111 IntelliCyt Technical Support IntelliCyt® Corporation 9620 San Mateo NE Albuquerque, NM 87113 USA www.intellicyt.com Telephone: 505-345-9075 opt 2 E-mail: support@intellicyt .com...

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