Polymer Laboratories PL-ELS 2100 Operator's Manual
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Polymer Laboratories PL-ELS 2100 Operator's Manual

Evaporative light scattering detector
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OPERATOR'S MANUAL
FOR
PL-ELS 2100/2100
EVAPORATIVE
LIGHT SCATTERING
DETECTOR
Version 1.5
Polymer Laboratories Ltd, Essex Road, Church Stretton, Shropshire SY6 6AX, UK
Tel +44 01694 723581, Fax +44 01694 722171, Service Tel +44 01694 724333
Polymer Laboratories Varian Inc, 160 Old Farm Road, Amherst, MA 01002, USA
Tel: +1 413 253 9554, Fax +1 413 253 2476
Polymer Laboratories BV, Herclesweg 8, 4338 PL Middleburg, The Netherlands
Tel +31 118 671 500, Fax +31 118 623 193
Polymer Laboratories GmbH, PEKA Park T5 001 Otto-Hesse Straße 19 D 64293 Darmstadt,
Germany Tel +49 06151 860690, Fax +49 06151 860670
Polymer Laboratories SARL, GVIO Parc de Marseille Sud, Impasse du Paradou, Bâtiment D5, 13276
Marseille, France. Tel + 33 0491 176400, Fax + 33 0491 176401

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Summary of Contents for Polymer Laboratories PL-ELS 2100

  • Page 1 Polymer Laboratories GmbH, PEKA Park T5 001 Otto-Hesse Straße 19 D 64293 Darmstadt, Germany Tel +49 06151 860690, Fax +49 06151 860670 Polymer Laboratories SARL, GVIO Parc de Marseille Sud, Impasse du Paradou, Bâtiment D5, 13276 Marseille, France. Tel + 33 0491 176400, Fax + 33 0491 176401...
  • Page 2 Practices, Protective earth connection Health & requirement on mains supply. Safety, 1.2, Power switch & coupler 1.4 & 1.5 accessible at all times. IEC 60825-1 classification of LED source. Version I May 2006 PL-ELS 2100/2100 Ice included (1.5) Reviewed SJOD...
  • Page 3 ______________________________________________________________________ Evaporative Light Scattering Detector PL-ELS 2100 Part number 0860-0110/0860-0240 PL-ELS 2100 Ice Part number 0860-01110/0860-01240 ______________________________________________________________________ conforms with the requirements of EC Directives 89/392, 91/368 & 89/336 by complying with the following Harmonised European Standards: Safety: EN61010 –...
  • Page 4 PL-ELS 2100/2100 Ice WARRANTY (Extract from General Conditions of Sale) Subject as hereinafter stated, if any goods supplied are proved to the reasonable satisfaction of the Seller to be defective in material or workmanship within a period of 12 months from the date of despatch and the Buyer notifies such defect to the Seller in writing...
  • Page 5 SAFETY Signs and Pictograms Used in this Manual WARNING: THE “WARNING SIGN” DENOTES A HAZARD. IT CALLS ATTENTION TO A PROCEDURE, PRACTICE WHICH, IF NOT CORRECTLY DONE OR ADHERED TO, COULD RESULT IN SEVERE INJURY OR DAMAGE OR DESTRUCTION OF THE INSTRUMENT. PLEASE DO NOT PROCEED BEYOND A WARNING SIGN UNTIL THE WARNING INDICATED CONDITIONS ARE FULLY UNDERSTOOD AND MET.
  • Page 6 Before using any cleaning or decontamination method except those recommended by Polymer Laboratories (see Appendix), please check with Polymer Laboratories that the proposed method will not damage the equipment. GENERAL PRECAUTIONS NOTE Perform periodic leak checks on supply lines.
  • Page 7 HEALTH AND SAFETY PL-ELS 2100/2100 Ice WARNING: This instrument should be used only in accordance with the instructions stated within this manual. Users should observe the following general safety precautions: • Ensure that the instructions within this manual are understood and carried out in the operation of the detector.

  • Page 8: Table Of Contents

    Interactive menu bar ................................. 15 Parameter Setting Menu..............................16 Real Time Monitoring............................... 18 Modes of Operation................................18 Using Methods..................................19 Error Conditions................................20 2.4 PL-ELS 2100/2100 I PC S ..........................20 OFTWARE Control Software-Overview ............................. 21 Configuring your PC and Detector..........................21 Connecting the Detector to your PC..........................
  • Page 9 ....................33 PGRADING THE IRMWARE USING ERMINAL CHAPTER 5 TROUBLESHOOTING............................38 ..............................38 NSTRUMENT RRORS ..............................39 ENERAL ROBLEMS APPENDIX 1....................................41 PL-ELS 2100/2100 I ..........................41 UICK UIDE APPENDIX 2....................................43 ..............................43 ETHOD ECORD HEET APPENDIX 3....................................44 PL-ELS 2100 T .............................44 EST PROCEDURE APPENDIX 4....................................

  • Page 10: Introduction

    The PL-ELS 2100/2100 Ice may be used alone, or as one of several detectors in a GPC or HPLC system. As the solvent or eluent is evaporated in the course of the analysis, the PL-ELS 2100/2100 Ice must be the last in series if used in conjunction with other detectors.

  • Page 11: Specifications

    Standby, Run Size Unpackaged 200x450x415 mm (wxdxh) Packaged 360x700x600mm (wxdxh) Weight Unpackaged 11kg (PL-ELS 2100) 13kg (PL-ELS 2100 Ice) Packaged 16 kg (PL-ELS 2100) 18kg (PL-ELS 2100 Ice) Document # 6/26069I Version 1.5 May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...

  • Page 12: Unpacking And Installation

    Any damage to the container or instrument should be reported immediately to your local distributor, or to Polymer Laboratories. It is recommended that the shipping container be kept, if possible, for re-shipment or return to a service centre.

  • Page 13: Site Preparation Check List

    If the extraction tube provided with the instrument is to be extended it is recommended that the diameter of the extension is increased to at least 50mm (2″) diameter tubing so the extraction quality is not inhibited Document # 6/26069I Version 1.5 May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...

  • Page 14: Connections

    Chapter 1 –General Information 1.6 Connections Figure 1.1 Front View of PL-ELS 2100/2100 Ice 1. Waste Outlet 2. Eluent Inlet 3. Keypad 4. Graphical Display Document # 6/26069I Version 1.5 May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...
  • Page 15 8. External Vapour Sensor 4. Connector control I/O –15 pin D type female 9. Panel Lock 5. Control Firmware flash upgrade 10. Exhaust port connector 11. Supply gas inlet Document # 6/26069I Version 1.5 May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...

  • Page 16: Power Connections

    ♦ If the voltage rating and fuses are correct for your power source, connect the power cable. 1.8 Extraction The PL-ELS 2100/2100 Ice is provided with tubing for venting the exhaust gases and vapours, and so does not need to be placed in a fume cupboard. Instead, the exhaust hose provided must be attached to the rear of the unit and vented to a fume hood or other disposal unit.

  • Page 17: Connections

    The serial RS232 connector provides a 24bit (10Hz) digital output for connection to a chromatographic acquisition device. The PL-ELS 2100/2100 Ice can also be controlled from a PC using the RS232 interface and the PL- ELS 2100/2100 Ice graphical control software from Polymer Laboratories Ltd. Refer to the control software online manual for the operating instructions.

  • Page 18: Gas Connection

    1.10 Precautions Extraction For correct operation and optimum performance of the PL-ELS 2100/2100 Ice, the unit must be vented to a fume hood or other means of vapour disposal using the exhaust hose provided. The exhaust hose should be routed upwards to allow condensed solvent to collect in the waste bottle at the front of the instrument.

  • Page 19: Flammable Solvents

    The pump stop facility should be connected directly to your pump or WARNING chromatography system controller when flammable solvents are used. Document # 6/26069I Version 1.5 Revision May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...

  • Page 20: Chapter 2 System Des Cription And General Operation

    A constant gas supply (volume and pressure), consistent eluent flow rate and proper venting of the exhaust should be ensured. Document # 6/26069I Version 1.5 Revision May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...

  • Page 21: Theory

    In order to decide which mechanism is predominantly responsible for the “scattering” observed in the PL-ELS 2100/2100 Ice, an estimate of the size of the particles involved compared to the wavelength of the incident light can be made: 0 45 σ...

  • Page 22: Operational Parameters

    The PL- ELS 2100 & 2100 Ice are designed to receive eluent flow rates up to 5 ml/min, at ambient temperatures even for 100% water. Document # 6/26069I Version 1.5 Revision May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...

  • Page 23: Instrument Controls

    The mains power switch is located at the rear of the instrument just above the mains cable. Re-booting the PL-ELS 2100/2100 Ice can be accomplished by switching off the detector, waiting a moment (typically until the cooling fan stops rotating), and then switching back on. As with any electronic device, if the unit is cold from shipment or storage in a non-heated area, it is recommended that the unit be allowed to come to room temperature before switching on.

  • Page 24: Keypad

    Ï The locked icon ( ) will be displayed with the instrument is controlled by the PC based software Document # 6/26069I Version 1.5 Revision May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...

  • Page 25: Parameter Setting Menu

    10mV, following a Pmt change and thus the recorded baseline position will remain unchanged. The magnitude of the baseline noise will be the obvious indication to the change in Pmt. Document # 6/26069I Version 1.5 Revision May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...
  • Page 26 Due to the averaging of data narrow peaks may become broadened when smoothing is applied N O T E NO SMOOTHING 5 SEC SMOOTHING Time (s) Figure 2.3.2 Example of smoothing data Document # 6/26069I Version 1.5 Revision May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...

  • Page 27: Real Time Monitoring

    Modes of Operation The PL-ELS 2100/2100 Ice can be operated in two modes; STANDBY and RUN, both of which are described below: To display the current mode and/or select a new mode, highlight the MODE function on the instrument display.

  • Page 28: Using Methods

    N O T E Using Methods On power up, the PL-ELS 2100/2100 Ice control system defaults to the parameters set in the default (‘XXX’) method. Modification of the default method is permitted using the front keypad on the instrument. Ten custom methods can be stored onboard for specific applications, but cannot be edited via the keypad on the front of the instrument.

  • Page 29: Error Conditions

    Table 2.2: Preset Methods Error Conditions The PL-ELS 2100/2100 Ice is equipped with a number of sensors and error checking facilities to ensure safe operation. If an error is detected the instrument gives an audible warning and a visible description of the error condition. In event of any error condition, the unit defaults into the STANDBY mode in which the heaters, LED &...

  • Page 30: Control Software-Overview

    Configuring your PC and Detector The PL-ELS 2100/2100 Ice requires a free Serial (RS-232) communications port (1 to 255) on your PC. Most computers are supplied with at least one serial port as standard. If your PC does not provide...

  • Page 31: Configuring The Els Control Software

    Serial Port COM 1. Operating the ELS Control Software Control of the PL-ELS 2100/2100 Ice is through the simple control window. Within the control window, the status of the detector is displayed, together with options to control the various instrument parameters.

  • Page 32: Control Software Features

    LED intensity can be adjusted via the Edit menu:- (EDIT / Instrument settings menu option). Changes made to these parameters need to be downloaded to the PL-ELS 2100/2100 Ice using the UPDATE button. Document # 6/26069I...

  • Page 33: Automation

    24-hour period. Using a time triggered event approach (based on the PC clock), the user can select a method of choice to be loaded and/or the PL-ELS 2100/2100 Ice status changed. When automation is selected (TOOLS / Automation menu option), the following set-up dialog is displayed.

  • Page 34: System Test

    Method Editor -Overview The PL-ELS 2100/2100 Ice can store 10 customs methods, which can be selected via the front of the instrument or via the control software. The method editor program allows the user to create, edit and download these custom methods for method development purposes or specific applications.

  • Page 35: Method Editor - Creating & Storing Methods

    Detector as a "Method Set". Each "Method Set" is stored as an individual binary format file with a File Extension (.ELS) By default, these Method Set Files are stored in the PL-ELS 2100 Method folder allocated at the time the software was installed (C:\PL-ELS2100\default) The loaded method set details can be printed out by selecting the print option from the FILE menu.

  • Page 36: Chapter 3 Set-Up

    Set-Up 3.1 General Considerations The PL-ELS 2100/2100 Ice instrument should be thought of as a detector like any other designed for liquid chromatography. The main distinguishing feature is the ability to evaporate the solvent from the column eluent. Therefore, normal system set-up precautions should be remembered when starting to use the instrument.
  • Page 37 ♦ Turn on the source gas to a pressure of about 60-100 psi. The gas will not flow though the detector until the instrument in set to RUN mode. ♦ Switch on the PL-ELS 2100/2100 Ice, and select a suitable method. ♦ Initiate heating by selecting RUN mode using the arrow keys on the front keypad.

  • Page 38: Optimisation Protocol

    The PL-ELS 2100 Ice can be operated at sub-ambient temperatures to improve the detection of semi- volatile compounds. In aqueous solvents, baseline noise may become too excessive at temperatures <15°C.
  • Page 39 In some cases, especially for volatile samples, it has been found that maximising the nebuliser temperature permits a lower evaporator temperature and thus an increase in sensitivity. Document # 6/26069I Version 1.5 Revision May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...

  • Page 40: Chapter 4 Routine Maintenance

    INSTRUMENT COVER IS OPENED. WARNING The PL-ELS 2100/2100 Ice is designed to be a low-maintenance instrument and does not generally require regular service. However, should the instrument performance deteriorate we recommend the following procedures to dry and clean the evaporator tube without dismantling the unit 4.1 Drying the Diffuser...

  • Page 41: Information For Service Personnel

    CONDITIONS BEFORE LEAVING INSTRUMENT WARNING UNATTENDED. If cleaning the unit does not cure the problems then consult Polymer Laboratories Ltd for further assistance. 4.3 Information for Service Personnel INSTRUMENT IS DOUBLED FUSED WARNING Heater PCB Fuses There are 3 fuses fitted to the main control PCB, one to protect each of the heater circuits FH 1 &...

  • Page 42: Upgrading The Firmware Using Terminal

    On completion of the programming close the Terminal program and turn off the instrument. 10. Move the serial cable back to the control connector and move the switch back to the up position (RUN). Document # 6/26069I Version 1.5 Revision May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...
  • Page 43 Chapter 4 – Routine Maintenance Figure 4.4.1 Terminal program Figure 4.4.2 Communications dialog Document # 6/26069I Version 1.5 Revision May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...
  • Page 44 Chapter 4 – Routine Maintenance Figure 4.4.3 Successful Communication Figure 4.4.4 Initialising the Programming Document # 6/26069I Version 1.5 Revision May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...
  • Page 45 Chapter 4 – Routine Maintenance Figure 4.4.5 Selecting the HEX file Figure 4.4.6 Downloading the Program Document # 6/26069I Version 1.5 Revision May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...
  • Page 46 Chapter 4 – Routine Maintenance Figure 4.4.7 Programming the EPROM Document # 6/26069I Version 1.5 Revision May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...

  • Page 47: Chapter 5 Troubleshooting

    Chapter 5 Troubleshooting If a problem is encountered Polymer Laboratories advises that a system test be performed via the PL- ELS 2100/2100 Ice PC control software (section 2.4) to ensure that the detector is working correctly. If there is an error or fault, please refer to the following table. If you follow the recommended course of action and the result is not satisfactory, then please direct the matter to Polymer Laboratories or your local distributor.

  • Page 48: General Problems

    Data acquisition leads not Ensure connectors to computer connected or integrator are sound • Light source inactive Check LED • Instrument in STANDBY mode Select RUN mode Document # 6/26069I Version 1.5 Revision May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...
  • Page 49 Ambient temperature too high Reduce ambient temperature to e.g. 10°C 25°C • Faulty Peltier cooler unit Consult Polymer Laboratories Ltd or your local agent if problem persists Document # 6/26069I Version 1.5 Revision May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...

  • Page 50: Pl-Els 2100/2100 Ice Quick User Guide

    PL-ELS 2100/2100 Ice Quick User Guide The following is intended to be a quick guide for operation of your PL-ELS 2100/2100 Ice. However, the Operator’s Manual must be read before attempting to operate the PL-ELS 2100/2100 Ice so that all the instrument controls are identified.
  • Page 51 Turn on the eluent flow and wait ~15 mins for the instrument to become primed and conditioned for optimum performance. On completion of your work, turn off the solvent pump and put the PL-ELS 2100/2100 Ice into STANDBY mode and turn off the power after 5 minutes.

  • Page 52: Method Record Sheet

    APPENDIX 2 Method Record Sheet Chromatography Conditions Eluent Eluent Method Evap Neb. Date Comments flow Temp Temp Flow Stored Document # 6/26069I Version 1.5 Revision May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...

  • Page 53: Pl-Els 2100 Test Procedure

    APPENDIX 3 PL-ELS 2100 Test procedure The PL-ELS 2100 is factory tested for sensitivity and baseline noise according to the following test procedure. Detector Settings Nebuliser Temperature: Gas flow rate: 1.6 SLM Evaporator Temperature Chromatography Conditions Sample: Glucose Concentration: 0.125 mg/ml...

  • Page 54: Pl-Els 2100 Ice Test Procedure

    APPENDIX 4 PL-ELS 2100 Ice Test procedure The PL-ELS 2100 Ice is factory tested for sensitivity and baseline noise, at two temperature settings, according to the following test procedure. Chromatography Conditions Sample: Glucose Concentration: 0.125mg/ml Eluent Water Injection Volume: 20µl...

  • Page 55: Spare Part Listing

    APPENDIX 5 Spare Part Listing The following list of spare parts was current at the time of issue of this manual. Please contact your local distributor or Polymer Laboratories for an up-to-date price list and availability. Description Part Number Manual for PL-ELS 2100/2100 Ice...

  • Page 56: Nitrogen Generator Specifications

    APPENDIX 6 Nitrogen Generator Specifications Document # 6/26069I Version 1.5 Revision May 2006 PL-ELS 2100/2100 Ice Operator’s Manual...

  • Page 57: Cleaning & Decontamination Procedures

    Identify and if possible correct source of the leak. Inspect cabling, parts and surfaces to determine whether any damage has occurred. If in doubt, contact Polymer Laboratories Service Dept for assistance. Allow the interior and exterior of the instrument to dry out completely before closing the cover and reconnecting power.

This manual is also suitable for:

Pl-els 2100 ice

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