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Dionex GP50 Operator's Manual

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GP50 GRADIENT PUMP
OPERATOR'S MANUAL
© 2001 Dionex Corporation
Document No. 031377
Revision 03
July 2001

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  • Page 1 GP50 GRADIENT PUMP OPERATOR'S MANUAL © 2001 Dionex Corporation Document No. 031377 Revision 03 July 2001...
  • Page 2 Dionex Corporation, 1228 Titan Way, Sunnyvale, California 94088-3603 U.S.A.

  • Page 3: Table Of Contents

    Contents 1 • Introduction Overview ..........1-1 About This Manual .
  • Page 4 GP50 Gradient Pump Rear Panel ..........2-18 Functional Description .
  • Page 5 Contents Example Methods ........3-13 3.4.1 Isocratic Method Example .
  • Page 6 GP50 Gradient Pump 5 • Service Cleaning and Replacing the Check Valves ..... . 5-1 Piston Seal Replacement ........5-4 Pump Piston Replacement .
  • Page 7 Contents B.2.4 DX-LAN Interface: BNC Connections (Optional) ..B-9 B.2.5 Waste Lines ........B-13 B.2.6 Eluent Inlet Line Connections.
  • Page 8 GP50 Gradient Pump C.2.5 DX-LAN Status ........C-18 C.2.6...

  • Page 9: Introduction

    It can also be used with non-Dionex modules that meet interface requirements for software, TTL, or relay control. The GP50 can be controlled locally, using the front panel keypad and display, or from a remote host computer with a Dionex DX-LAN™ interface installed and PeakNet software installed on the host computer.

  • Page 10: About This Manual

    Chapter 3 Provides an overview of GP50 operation, including how to Operation and create, edit, and run methods from the GP50 front panel. Maintenance Lists routine preventive maintenance procedures. Chapter 4 Lists problems and presents step-by-step procedures for Troubleshooting how to isolate and eliminate them.

  • Page 11: Safety Messages And Notes

    This manual contains warnings and precautionary statements that, when properly followed, can prevent personal injury and/or damage to the GP50. Safety messages appear in bold type and are accompanied by icons, as shown below. Indicates an imminently hazardous situation which, if not avoided, will result in death or serious injury.

  • Page 12: Safety Labels

    Safety Labels The TUV GS, C, US Mark safety label and the CE Mark label on the GP50 indicate that the GP50 is in compliance with the following standards: EN 61010-1:1993 (safety), CAN/CSA-C22.2 No. 1010.1-92 (safety), UL 3101-1/10.93 (safety), EN 50082-1:1992 (susceptibility), and EN 55011:1991 (emissions).

  • Page 13: Description

    2 • Description The GP50 Gradient Pump consists of two units (see Figure 2-1). The upper unit houses the electronics components and the lower unit houses the pump heads and other mechanical pump assemblies. P um p H eads Figure 2-1. GP50 Enclosure...

  • Page 14: Front Control Panel

    GP50 Gradient Pump Front Control Panel The control panel on the upper door of the pump enclosure contains a liquid crystal display (LCD), a membrane keypad, and the actuator for the main power switch (see Figure 2-2). The door opens to provide access to the electronics chassis (see Section 2.2).

  • Page 15: Control Panel Keypad

    (for tilting the door) the contrast) the panel) Figure 2-2. GP50 Display and Keypad Layout 2.1.1 Control Panel Keypad Use the keypad to directly control pump operation, as well as to create and modify programmed series of timed events, called methods. In...
  • Page 16 Insert steps in any order. When you press Enter, they will be automatically reorganized in the correct chronological order. Table 2-1. GP50 Front Panel Buttons Doc. 031377-03 7/01...
  • Page 17 Changes the method clock time to INIT , causing the initial conditions specified by the method to occur. This button functions only when the GP50 is under Method control (see Section 2.9.3). If the method is running, it continues running. If the method is in Hold, the method clock executes the initial conditions and holds.
  • Page 18 From a menu screen, pressing a numeric button opens the corresponding screen. Saves and/or executes changes made in entry fields. If a menu screen is displayed, pressing Enter opens the highlighed screen. Table 2-1. GP50 Front Panel Buttons (Continued) Doc. 031377-03 7/01...

  • Page 19: Display Screens

    Use the screen to select operating MAIN parameters, such as the flow rate and the percentages of eluents to run. To access other GP50 screens, press the Menu button to display the MENU (see Figure 2-5).

  • Page 20: Electronics Chassis

    The chassis includes several electronics cards (printed circuit boards) that are used to control the pump. Connectors on the cards also allow communication between the pump and other Dionex chromatography modules. Figure 2-6 shows the electronics components with the upper door open. To open the door, pull on the tab located to the right of the main power actuator (see Figure 2-2).
  • Page 21 The leak control cable from the LC10 Chromatography Organizer or LC20 Chromatography Enclosure, connects to the connector in LC LEAK slot 1. When a leak occurs in the LC10 or LC20 it is reported to the GP50. NOTE The LC25 Chromatography...
  • Page 22 4. Green indicates normal operation. Red indicates a power fault. The GP50 will enter its diagnostic state and inhibit all other controls until the fault is corrected. If this occurs, turn off the power for a few seconds and then turn it back on.

  • Page 23: Mechanical Chassis

    Les flèches sur l'étiquette indiquent des pièces mécaniques mobiles qui posent un danger de pincement lorsque le GP50 est sous tension et le tiroir mécanique est ouvert. N'utilisez jamais le GP50 avec le tiroir du châssis mécanique ouvert.

  • Page 24: Mechanical Components

    Figure 2-7 shows the mechanical components located behind the lower door of the enclosure. Pressure Transducer Pressure Transducer Pum p Waste Valve H eads Prim ing B lock Eluent M anifold Proportioning Figure 2-7. GP50 Mechanical Components 2-12 Doc. 031377-03 7/01...

  • Page 25: Pump Eluent Manifold

    2.4.1 Pump Eluent Manifold Eluent lines A through D are routed through the rear of the GP50 to the vacuum degas pump (if installed) (see Section 2.5) and then to the rear of the proportioning valve. If the vacuum degas pump is not installed, the eluent lines are connected directly to the proportioning valve.

  • Page 26: Pump Heads

    GP50 Gradient Pump 2.4.2 Pump Heads There are two types of pump heads: standard bore and microbore. The table below summarizes the features and operating conditions for each type. Pump Head Piston Pump Flow Rate Maximum Type Volume Operation (mL/min)*...

  • Page 27: Pump Priming Block

    2 • Description 2.4.4 Pump Priming Block The priming block “tee” directs the flow of eluent from the manifold into the pump heads. The priming block is also used for rapid removal of air from the system. Refer to Section B.2.8 for instructions on priming the pump heads. 2.4.5 Pressure Transducer From the priming block, the liquid stream is directed to the inlet check...

  • Page 28: Vacuum Degas Pump Assembly (Optional)

    The electronics required to operate the vacuum pump Fittings, tubing, and other accessories By default, the degas pump turns on for 2 minutes when the GP50 power is turned on. Thereafter, the pump turns on for 30 seconds at 10-minute intervals. The...

  • Page 29: Eluent Reservoirs

    034581) for installation details. EO1 Eluent Organizer (Optional) The Dionex EO1 Eluent Organizer (P/N 044125) holds eluent reservoirs in a liner that contains spills and leaks. The EO1 can also be used to pressurize reservoirs. Up to two optional E01 Eluent Organizers can be places on top of the system 2-17 Doc.

  • Page 30: Rear Panel

    2-Liter plastic 2-Liter glass Rear Panel The rear panel contains the main power receptacle and fuses. It also includes a DX-LAN connector for interfacing the GP50 with the PeakNet workstation. The rear panel is illustrated in Figure B-1. Functional Description 2.9.1...

  • Page 31: Local And Remote Modes

    Local and Remote Modes Local Mode When the GP50 power is turned on, the pump is in Local mode. In Local mode the pump accepts operating commands from two sources: Direct input from the front panel keypad and screens. With direct input, all GP50 operating functions are available.

  • Page 32: Method Control

    When the GP50 is controlled by PeakNet 5, select the Locked Remote Start option from the PeakNet 5 Run program to operate in the Locked Remote mode. To return the GP50 to Local mode, clear the Start option or turn the GP50 power off and then on.
  • Page 33 (initial condition). Step 2 always starts at INIT TIME = 0.0 The GP50 can store up to 100 separate methods (0 through 99) in memory. Methods are retained in memory even after the pump is powered down. The total number of methods that can be stored in memory depends on the length of each method and the amount of available memory;...

  • Page 34: Eluent Delivery

    Eluent Delivery Isocratic Eluent Run The simplest use of the GP50 is for the delivery of an isocratic (unchanging) mixture of one or more eluents. If more than one eluent is selected, the pump delivers a proportional mixture of the eluents based on the percentage of each eluent selected.
  • Page 35 2 • Description Curve numbers are defined as follows: Curve Number Gradient Type 1, 2, 3, 4 Convex Linear (power-up default) 6, 7, 8, 9 Concave NOTE A curve number in a step instructs the pump to use the selected curve number when moving from the previous step to that step.
  • Page 36 GP50 Gradient Pump 2-24 Doc. 031377-03 7/01...

  • Page 37: Operation And Maintenance

    The GP50 with the optional vacuum degas pump assembly provides continuous on-line vacuum degassing of up to four eluents. If the GP50 is not equipped with the vacuum degas assembly, manually vacuum-degas eluents daily, as described below, and store them in pressurized reservoirs.

  • Page 38: Filter Eluents

    3.1.3 Pressurize Eluent Reservoirs The GP50 Gradient Pump is capable of operation with or without head pressure on the eluent. Pressurization of the eluent reservoirs, if used, should be with filtered inert gas (preferably helium). Refer to the Pressurizable Reservoir Installation Instructions for details.

  • Page 39: Start-Up

    If one of the tests fails, the screen DIAGNOSTIC TEST displays instead. See Section C.2.7 if this occurs. When the GP50 power is turned on, the injection valve is initialized to the Load position. 2. Press to start the pump flow.

  • Page 40: Selecting The Pressure Limits

    GP50 Gradient Pump 3.1.5 Selecting the Pressure Limits The high and low pressure limits automatically stop the pump if a system malfunction occurs, such as overpressurization caused by a blockage or low pressure caused by a leak downstream from the pump.

  • Page 41: Selecting The Operating And Control Modes

    LOCKED RMT the GP50 to , either clear the Connect check box on the LOCAL PeakNet 6 control panel or turn the GP50 power off and then on. If the GP50 is controlled by PeakNet 5, select LOCAL REMOTE on the screen.

  • Page 42: Running Under Direct Control (Local Mode)

    GP50 Gradient Pump Running Under Direct Control (Local Mode) Direct Control, Local mode is used most often when a PeakNet workstation is not configured. Direct Control Example Specify an isocratic mixture of 60% eluent A, 25% eluent B, and 15% eluent C to be pumped at 2.0 mL/min (see Figure 3-3).

  • Page 43: Running Under Method Control (Local Mode)

    This allows the flow rate to stabilize. Running Under Method Control (Local Mode) This section provides general instructions on how to create, edit, and run methods from the GP50 front panel. For step-by-step examples, see Section 3.4. When entering parameters on the screen, observe these guidelines:...

  • Page 44: Creating A New Method

    GP50 Gradient Pump gradient when moving to the step from the one preceding it (see Section 2.9.4). The default is curve 5 (linear). NOTE Because there are no previous steps for INIT TIME=0.0 curve numbers are not entered for them.
  • Page 45 3 • Operation and Maintenance R U N 0 M ETH O D EDIT SAVE TO 0 - 5000 LIM ITs TIM E FLO W IN IT 100.0 L 1.00 > > 0.00 > > H elp M essage Figure 3-5. Method Screen: Creating a New Method 4.

  • Page 46: Running A Method

    GP50 Gradient Pump 3.3.2 Running a Method 1. If the pump motor is off, press to turn on the motor. Off/On 2. Go to the screen and if necessary change the MAIN DETAIL operating and control modes to (see LOCAL METHOD Section 3.1.6).

  • Page 47: Editing A Method

    3 • Operation and Maintenance 3.3.3 Editing a Method Existing methods can be modified by changing, adding, or deleting steps and parameters. Changes can be made when the method clock is stopped or running. If the method you are editing is currently running, the changes are stored in memory and implemented when you save the method.

  • Page 48: Deleting A Method

    GP50 Gradient Pump 3.3.4 Deleting a Method To delete an entire method, move the cursor on the screen to the METHOD step and press twice. Delete INIT 3.3.5 Changing the Running Method To change from the method currently running to a different method, go to...

  • Page 49: Example Methods

    3 • Operation and Maintenance Example Methods The examples in this section provide step-by-step instructions for creating three types of methods: isocratic, linear gradient, and curved gradient. The last example demonstrates how to edit a running method. For all of the examples, set the pump to mode, control (see LOCAL...

  • Page 50: Linear Gradient Method Example

    GP50 Gradient Pump 3. Move the cursor to and press to save the method. Enter SAVE TO 4. Move the cursor to , enter the method number (1, in this case) and press to select the programmed method. If the pump motor is...
  • Page 51 3 • Operation and Maintenance Figure 3-7. Linear Gradient Method Profile 1. Go to the screen and enter a method number in the METHOD EDIT field (2, for example). If Method 2 currently exists and you want to retain it, enter a new, unused, method number in the field.
  • Page 52 GP50 Gradient Pump 3. Move the cursor to the field of the step and press Enter TIME = 0 100% of eluent A is automatically filled in. 4. Move the cursor to the line below and enter 5 to store a step TIME = 0, minutes.
  • Page 53 3 • Operation and Maintenance 6. Move the cursor to the next line. Enter 15 in the field. Move the TIME cursor to the field and enter 50, followed by 50 in the field. Beginning with the method step immediately preceding this one ), the pump begins adding eluent C, starting with 0%.
  • Page 54 GP50 Gradient Pump 11. Move the cursor to the field and press to save the Enter SAVE TO method to memory. Figure 3-11 illustrates the completed method. M ETH O D ED IT SAVE TO R U N 0 LIM ITs...

  • Page 55: Curved Gradient Method Example

    3 • Operation and Maintenance 3.4.3 Curved Gradient Method Example When attempting to optimize the separation of a single component in a complex mixture, it is often helpful to employ paired segments of a curved gradient. For example, if the analyte is an oligonucleotide that elutes with 0.32 M NaCl in a linear gradient run, you can move potentially interfering components of the sample away from the target oligonucleotide by first programming a segment from 0% to 32% of a 1 M...
  • Page 56 GP50 Gradient Pump Figure 3-13. E1 and E2 Curves The following summarizes the curved gradient method example steps: Create Method 3 to begin under isocratic conditions with 100% eluent A (25 mM Tris buffer, pH 8.0) at 1.5 mL/min. After 1 minute, begin a convex addition of eluent B (25 mM Tris buffer, pH 8.0, containing 1.0 M NaCl) while decreasing eluent A...
  • Page 57 3 • Operation and Maintenance Figure 3-14 illustrates the screen as it appears so far. You METHOD can now begin entering the method steps that will generate the curved gradient profile (see Figure 3-13). M ETH O D EDIT SAVE TO R U N 0 LIM ITs 0 - 5000...
  • Page 58 GP50 Gradient Pump M ETH O D EDIT SAVE TO R U N 0 LIM ITs 0 - 5000 TIM E FLO W 100.0 IN IT L 1.50 > > 0.00 100.0 > 1.00 100.0 > 3.50 68.0 32.0 H elp M essage Figure 3-15.

  • Page 59: Editing A Running Method Example

    3 • Operation and Maintenance 3.4.4 Editing a Running Method Example After entering a method, you can modify it by changing, adding, or deleting steps. The example describes how to make the following changes to Method 2, the linear gradient example (see Section 3.4.2): Change the eluent composition at from 50% eluent B and TIME = 15...
  • Page 60 GP50 Gradient Pump This example assumes that the example Method 2 is currently running. 1. Go to the screen and enter 2 in the field. METHOD EDIT 2. Move the cursor down through Method 2 until you reach the step. Move the cursor to and enter 45.

  • Page 61: Routine Maintenance

    1. Open the lower pump door and locate the two rinse ports on the front of each of the pump heads. Figure 3-19 shows the rinse port connections. 2. Install a rinse waste tube (P/N 054418), provided in the GP50 Ship Kit, onto each head (see Figure 3-19). Rinse Ports...
  • Page 62 Wipe up liquid spills and rinse dried reagents off the pump components with deionized water. Dionex recommends thoroughly flushing the vacuum degas assembly with deionized water after each use. Flushing chemicals out of the degas chambers and tubing avoids crystallization in the membrane pores.

  • Page 63: Periodic Maintenance

    Before a shutdown of more than three days, reduce the pressure on the eluent reservoir(s) to approximately 21 KPa (3 psi). Shut down the pump by turning off the main power on the GP50. 3-27 Doc. 031377-03 7/01...
  • Page 64 GP50 Gradient Pump 3-28 Doc. 031377-03 7/01...

  • Page 65: Troubleshooting

    This chapter is a guide to troubleshooting minor problems that may occur while operating the GP50 Gradient Pump. To use this guide, turn to the section that best describes the operating problem. There, possible causes of the problem are listed in order of probability.
  • Page 66 Les flèches sur l'étiquette indiquent des pièces mécaniques mobiles qui posent un danger de pincement lorsque le GP50 est sous tension et le tiroir mécanique est ouvert. N'utilisez jamais le GP50 avec le tiroir du châssis mécanique ouvert.

  • Page 67: Pump Will Not Start

    2. If a non-zero flow rate is displayed and the keypad LED is on, verify that the electrical cables in the mechanical chassis are properly installed. a. Turn off the GP50 power. b. Using a 7-mm open-end wrench or your fingers, loosen the lock on the mechanical chassis drawer.
  • Page 68 GP50 Gradient Pump Low pressure limit was tripped. The following message is displayed: Low Pressure Limit Violation 1. Verify that eluent is present in the channel selected. If the eluent reservoir is empty, refill it or select a channel which does have eluent. Prime the pump (see Section B.2.8) before resuming operation.
  • Page 69 DSP communication fails, DSP does not acknowledge, etc. If one of these messages is displayed, follow the procedure below: 1. Turn off the GP50 power. 2. Verify that the DSP card is present and correctly installed in slot 1 of the electronics chassis (see Figure 2-6).
  • Page 70 1. Turn off the GP50 power. 2. Verify that the cables connected to the DSP card in the electronics chassis (see Figure 2-6) are seated properly. 3. Turn on the GP50 power. If the error message reappears, notify Dionex Technical Support. Doc. 031377-03 7/01...

  • Page 71: Liquid Leaks/Leak Alarm

    Inspect the pressure transducer. If the waste valve is the source of the leak, replace the waste valve O-ring (see Section 5.4). If the leak is from the rear of the transducer, contact Dionex Technical Support. Priming valve Tighten any leaking fittings just enough to stop the leak. If this does not stop the leak, replace the fittings and/or tubing making the connection.

  • Page 72: Noisy Pump Motor

    Activating the current limit is harmless, but if it occurs frequently, even at low speeds and/or pressures, the bottom plate assembly (P/N 045670) needs to be replaced. Call Dionex Technical Support for assistance. Pressure servo oscillation...

  • Page 73: Vacuum Degas Pump Does Not Run

    Figure 2-6) and in the mechanical chassis are properly connected. To check the mechanical chassis connections: a. Turn off the GP50 power. b. Using a 7-mm open-end wrench or your fingers, loosen the lock on the mechanical chassis drawer. The lock is on the lower right side of the chassis, between valves 3 and 4 (see the label on the inside of the lower door).

  • Page 74: Vacuum Degas Pump Calibration Fails

    4. Push the mechanical chassis drawer back in place, making sure the cables are not pinched. Retighten the drawer lock. 5. Turn on the power. 6. Retry the calibration. If the message reappears, contact Dionex Technical Support. 4-10 Doc. 031377-03 7/01...

  • Page 75: Vacuum Degas Pump Low Vacuum

    4 • Troubleshooting Vacuum Degas Pump Low Vacuum The GP50 monitors the degas vacuum reading every 1 min. If the degas vacuum is lower than the monitoring value, the degas pump turns on. When the pump turns off, if the vacuum reading is 2000 counts or more lower than...

  • Page 76: Poor Chromatographic Reproducibility

    GP50 Gradient Pump 4.10 Poor Chromatographic Reproducibility Liquid lines incompletely flushed after an eluent change Attach a syringe to the priming block valve (see Figure B-5) and draw at least 2.5 mL (20 mL if the vacuum degas pump is installed) of the new eluent through the liquid lines before beginning operation.

  • Page 77: Service

    Any procedures not included here, including electronics-related service procedures, must be performed by Dionex personnel. For assistance, contact Dionex Technical Support. In the U.S., call 1-800-346-6390. Outside the U.S., call the nearest Dionex office. Before replacing any part, refer to the troubleshooting information in Chapter 4 to isolate the cause of the problem.
  • Page 78 GP50 Gradient Pump Outlet Pressure Check Transducer Valve To Colum n Pum p Head Inlet To Waste Check Valve Figure 5-1. Pump Heads and Liquid Lines (PEEK) Pressure Tran sducer To C olum n O utlet C heck Valve Pu m p...
  • Page 79 8. Reconnect the liquid lines. 9. Turn on the GP50 power. 10. Prime the pump (see Section B.2.8). If the pump will not prime and you have eliminated all other possible causes of the problem, replace the check valve cartridge.

  • Page 80: Piston Seal Replacement

    GP50 Gradient Pump Piston Seal Replacement A damaged seal allows leakage past the piston and then through the rinse ports in the front of the pump heads. The pump may be difficult to prime, flow rates will be unstable, and baseline noise may be observed.
  • Page 81 5 • Service O u tlet Check Valve A ssem bly Head Piston Seal Rin se S eal Piston G uid e O -Ring Inlet Ch eck Su pport Washer Valve A ssem bly Backup Washer Piston Figure 5-3. Pump Head Assembly Component Standard Standard...
  • Page 82 GP50 Gradient Pump 7. To remove the piston guide and seal: a. Fill the head cavity with deionized water by injecting through either the piston opening or the inlet check valve. b. Reinsert the piston approximately 1/8-in into the seal (see Figure 5-4).
  • Page 83 5 • Service 8. To install the new seal and reinstall the piston guide: a. Push the piston through the piston guide and the new seal. Then, insert the piston, piston guide, and seal into the pump head until the seal contacts the bottom of the counterbore (see Figure 5-5, View A).

  • Page 84: Pump Piston Replacement

    GP50 Gradient Pump 9. Dionex recommends reinstalling the head and piston as a single assembly so that the piston centers itself. To do this: a. Press the backup washer into the head until it is flush with the indented surface of the head.
  • Page 85 6. Replace the piston and the piston seal. Broken or scratched pistons can damage the piston seal. 7. Dionex recommends reinstalling the head and piston as a single assembly so that the piston centers itself. To do this: a. Press the backup washer into the head until it is flush with the indented surface of the head.

  • Page 86: Pressure Transducer Waste Valve O-Ring Replacement

    GP50 Gradient Pump Pressure Transducer Waste Valve O-Ring Replacement A damaged O-ring causes leakage around the base of the pressure transducer waste valve knob. 1. Turn off the main power switch, to ensure you do not unintentionally start the GP50.

  • Page 87: Proportioning Valve Replacement

    (which may cause high backpressure). 1. Turn off the main power switch, to ensure you do not unintentionally start the GP50. 2. Turn off the pressure on the eluent reservoirs and allow them to vent. 3. Follow these steps to disconnect the proportioning valve electrical connector from the distribution card.
  • Page 88 GP50 Gradient Pump 5. Loosen the screws securing the proportioning valve/manifold assembly to the bulkhead (see Figure 5-7). Pull the valve/manifold assembly forward and disconnect the eluent lines on the manifold inlet. Remove the valve/manifold assembly completely from the bulkhead.

  • Page 89: Changing Main Power Fuses

    5 • Service Changing Main Power Fuses 1. Turn off the main power. HIGH VOLTAGE—Disconnect the main power cord from its source and also from the rear panel of the GP50. HAUTE TENSION—Débranchez cordon d'alimentation principal de sa source et du panneau arrière du GP50.
  • Page 90 GP50 Gradient Pump 5-14 Doc. 031377-03 7/01...

  • Page 91: A • Specifications

    A • Specifications A.1 Electrical 100 Vac to 240 Vac, 50/60 Hz; 2.5 amps. The GP50 power Main Power Requirements supply is main voltage auto-sensing and requires no manual adjustment. Fuse Two 3.15 amp fast-blow IEC127 fuses (P/N 954745) Requirements A.2 Environmental...

  • Page 92: Hydraulics

    0. to 35 MPa (0 to 5000 psi) in increments of 0.05 MPa Low Pressure Limit (7.25 psi); trips after a time-out of 0.4 mL for the standard bore GP50 or 0.1 mL for the microbore GP50 Standard bore pump head: <700 µL Delay Volume Microbore pump head: <300 µL (No Mixer) Doc.

  • Page 93: Control

    A • Specifications A.6 Control Limited remote operation via TTL-input logic level, and TTL- Remote output and relay contact closures, or full remote programming and control via the DX-LAN interface. Local (Front Panel) Methods: Stores up to 100 separate methods (00 through 99), The actual number of stored methods depends on available memory.
  • Page 94 GP50 Gradient Pump Doc. 031377-03 7/01...

  • Page 95: B • Installation

    Soyez prudent lorsque vous soulevez le GP50: il pèse 19 kg (42 lb). Allow at least 6 cm (2.5 in) free space behind the GP50 for connections and ventilation. The GP50 Gradient Pump is capable of operation with or without head pressure on the eluent.

  • Page 96: Installation Instructions

    GP50 Gradient Pump B.2 Installation Instructions The GP50 Ship Kit contains items necessary for completing the installation. GP50 Gradient Pump Version Ship Kit Standard bore/microbore with PEEK components P/N 054621 Standard bore/microbore with stainless steel components P/N 054627 B.2.1 Power Connection SHOCK HAZARD—To avoid electrical shock, a grounded...
  • Page 97 B • Installation The GP50 power supply is auto-sensing, so no adjustment is required to select the line voltage. There are two ways to control power to the GP50. Control from the GP50 To implement: Connect a modular power cord (IEC 320 C13) from the GP50 main power receptacle (see Figure B-1) to a grounded, single-phase power source.

  • Page 98: Electronics Chassis Connections

    1. Route the leak sensor cable from the rear of the LC10 Chromatography Organizer or the LC20 Chromatography Enclosure through the GP50 upper chase (see Figure B-1). Connect the cable to connector in slot 1 of the pump electronics chassis (see LC LEAK Figure B-2).
  • Page 99 B • Installation LC25 Connections Route the electronics injection valve cable from the rear of the LC25 Chromatography Oven through the GP50 upper chase(see Figure B-1). Connect the cable to the connector in slot 1 of the pump LC AIR electronics chassis (see Figure B-2).

  • Page 100: Dx-Lan Interface: 10Base-T Connections (Optional

    Standard anti-static procedures must be observed when installing the interface card or handling the CPU card. To prevent damage to the GP50, turn off the main power before installing the pump interface card. After confirming that the LED on the CPU card is off (not green or red), unplug the power.
  • Page 101 B • Installation 1. To replace an existing pump interface card, remove the DX-LAN cable from the 10BASE-T DX-LAN connector on the GP50 rear panel (see Figure B-1). 2. Remove any TTL/Relay plugs from the connectors at slot 4 of the electronics chassis (see Figure B-2).
  • Page 102 3. Connect the other end of the cable to the 10BASE-T DX-LAN connector on the GP50 rear panel (see Figure B-1). 4. Connect a 10BASE-T cable (P/N 960281) from a 10BASE-T port on the hub to the 10BASE-T port on the host computer’s internal DX- LAN card.

  • Page 103: Dx-Lan Interface: Bnc Connections (Optional

    Section B.2.3. In order to communicate with a host computer running Dionex chromatography software, the GP50 must contain a pump interface card (P/N 046341) and a coaxial DX-LAN cable (P/N 960405) must be connected to the BNC tee connector (P/N 921914) on the rear panel.
  • Page 104 GP50 Gradient Pump To prevent damage to the GP50, turn off the main power before installing the pump interface card. After confirming that the LED on the CPU card is off (not green or red), unplug the power. Do not rely on the front panel power switch.
  • Page 105 50 ohm terminator resistor plug (P/N 921034) on the remaining port of the BNC tee connector. (These terminator resistor plugs are shipped with Dionex chromatography software.) If the pump is not the last module in the network, connect the cable from the next module to the BNC tee.
  • Page 106 GP50 Gradient Pump 4. Plug the free end of the coaxial DX-LAN cable into one of these: The BNC connector on another instrument, or The BNC port on the rear panel of the “combo” 10BASE-T Ethernet hub (P/N 056909). If the connection terminates at the hub, install a BNC terminator (included with the hub) on the BNC tee connector.

  • Page 107: Waste Lines

    These lines are factory-installed and routed to the rear panel. Place the free end of both waste lines into a single waste container. Position the waste container below the level of the GP50 to maintain a positive siphon (see Figure B-3). Rear...

  • Page 108: Eluent Inlet Line Connections

    GP50 Gradient Pump B.2.6 Eluent Inlet Line Connections Eluent lines A through D are plumbed to the pump at the factory. To complete the installation, attach the free end of each line to the corresponding eluent reservoir. B.2.7 Eluent Outlet Line Connections Connect the eluent line exiting the pressure transducer (see Figure 5-1) to the gradient mixer.

  • Page 109: Piston Seal Wash Connections (Optional

    1. Fill a wash bottle with deionized water and place the bottle above the GP50. (During operation, gravity maintains flow through the pump heads.) 2. Cut the clear 1.5-mm (0.06-in) ID tubing (P/N 055847) into three...
  • Page 110 GP50 Gradient Pump Elevate bottle above the pum p. Pum p Heads Silicone Tubing (P/N 055847) 115 cm (46 in) Silicone Tubing (P/N 055847) 15 cm (6 in) B arbed Fittings (4) (P/N 037431) Silicone Tubing (P/N 055847) 150 cm (60 in)

  • Page 111: Priming The Pump

    7. Draw the syringe back to pull eluent through the flow path. It may take several syringe draws to remove all air or previous eluents from the tubing. NOTE If the GP50 includes a vacuum degas assembly, draw an additional 17 mL of eluent through the priming block.
  • Page 112 GP50 Gradient Pump Pressure Transducer Waste Valve Prim ing Block Valve Luer Port Syringe (P/N 054578) Prim ing Block Figure B-5. Priming the Pump 8. Return to Step 2. Select another eluent and repeat the priming procedure. 9. When the manifold has been primed with each eluent, turn the priming block valve counterclockwise until closed (see Figure B-5).
  • Page 113 B • Installation 4. Press on the GP50 front panel. The pump will pump at Prime 9.9 mL/min for a standard bore pump and 2.5 mL/min for a microbore pump. NOTE The vacuum degas assembly contains 17 mL of eluent. If this option is installed, allow several extra minutes of prime time to purge air and previous eluent from the degas assembly.

  • Page 114: Automatic Srs Power Control (Optional

    TTL connections can be used to automatically switch off the power to a Self- Regenerating Suppressor (SRS) when the GP50 flow stops. In this setup, the GP50 sends a TTL signal to the detector and then, after a 5-minute delay, the detector shuts off the SRS power. The 5-minute delay allows momentary flow interruptions without disturbing the SRS.
  • Page 115 B • Installation the GP50 and into the detector upper chase. Plug the other end of the cable into on the detector (see Figure B-7), maintaining the wire orientation TTL3 IN described in Step 4. Figure B-7. TTL Connections for SRS Power Control...
  • Page 116 GP50 Gradient Pump B-22 Doc. 031377-03 7/01...

  • Page 117: C • Display Screens

    C • Display Screens This appendix illustrates and describes all of the screens that can be displayed on the GP50 front panel. There are three types of screens: operational, diagnostic, and calibration (see Figure C-1). Operational screens allow you to select default pump operating parameters, and to create, edit, and run methods that use timed events to control pump operation.

  • Page 118: Operational Screens

    GP50 Gradient Pump C.1 Operational Screens C.1.1 Menu of Screens provides top-level access to GP50 display MENU of SCREENS screens. M EN U of SC R EEN S M AIN SC REE N M O D U LE SET-UP PU M P O PTIO NS...

  • Page 119: Main Screen

    C • Display Screens C.1.2 Main Screen screen displays automatically after power-up. Use the MAIN MAIN screen to: Select the operating mode and control mode. Select basic operating parameters for Direct control mode or display the status of operating parameters when a Method is running. Select the method number to run.
  • Page 120 - From PeakNet 6, clear the Connect check box on the PeakNet 6 control panel. - From PeakNet 5, clear the Locked Remote Start option in the Run program. - Turn off the GP50 power and turn it on again. DIRECT Selects the control mode: CONTROL/...

  • Page 121: Detail Screen

    (see Section C.1.2), as well as the additional parameters described below. Use the screen to: DETAIL Display detailed information about the status of GP50 operating parameters. Set parameters for Direct Control operation. D E TA IL S C R E E N TTL1 100.0 % A...

  • Page 122: Method Screen

    GP50 Gradient Pump C.1.4 Method Screen Use the screen to select, edit, and save methods. METHOD M ETH O D ED IT SAVE TO RU N 10 LIM ITs 0 - 5000 PS I TIM E FLO W IN IT 25.0...
  • Page 123 C • Display Screens %A, %B, %C, %D The percentage of each eluent occurring at the start of the step. The total of all eluents must equal 100%. The gradient curve type ( through ) to use when moving from the previous step to the current step. See Section 2.9.4 for an explanation of the curve types.

  • Page 124: Method Extension Screen

    GP50 Gradient Pump C.1.5 Method Extension Screen NOTE The extension screen can be accessed only METHOD from the screen (see Section C.1.4). To METHOD return to the screen, move the cursor to METHOD the left edge of the screen and press the left arrow.

  • Page 125: Degas Options

    : The pump is always off. ALWAYS OFF : The pump is always on. This ALWAYS ON setting is reserved for test purposes by Dionex Service Representatives. The length of time the pump runs at power-up START-UP (2-5 min).
  • Page 126 GP50 Gradient Pump The GP50 monitors the degas vacuum reading every one minute. If the degas vacuum reading is at or below the alarm threshold value (2000 counts below the calibration threshold value), the following message displays: LOW VACUUM ALARM!!

  • Page 127: Module Setup

    C • Display Screens C.1.7 Module Setup Use this screen to adjust the display backlight, and to toggle the key actuation and error tones on or off. Adjustments made in this screen will be the default until new adjustments are made. M O D U LE S ETU P D ISP LAY PA N E L B A C K LIG H T: M ED IU M...

  • Page 128: Pump Options

    GP50 Gradient Pump C.1.8 Pump Options Use the screen to specify the type of pump head installed PUMP OPTIONS and to set pump operation parameters. P U M P O P TIO N S P U M P H E A D M ATE R IA L:...

  • Page 129: Time Function In

    NORMAL EDGE, INVERTED , or EDGE, NORMAL PULSE INVERTED PULSE that corresponds to the signal type of the controlling device. The default mode, normal edge, is compatible with the TTL output signals provided by Dionex modules. C-13 Doc. 031377-03 7/01...

  • Page 130: Diagnostic Screens

    Figure C-11. Diagnostic Menu There are two ways to select a screen from the menu: Press the numeric button on the GP50 front panel keypad that corresponds to the screen number on the menu. For example, press to select and display the...

  • Page 131: Power-Up Screen

    POWER-UP installed, the revision levels of the Moduleware and BIOS installed, and the ID number of the DX-LAN interface (if connected). screen is automatically displayed when the GP50 power POWER-UP is turned on. G P50 G RA DIEN T PU M P PU M P HE AD VO LUM E 100 uL M O D U LEWAR E R EV n.nn...

  • Page 132: Elapsed Time

    GP50 Gradient Pump C.2.3 Elapsed Time Use the screen to check for how long various GP50 ELAPSED TIME components have been in use. The status of each parameter updates in real time. ELAPSED TIM E R ESET M O DU LE O N: nnnnnnnn hours...

  • Page 133: Dsp Status

    C • Display Screens C.2.4 DSP Status Use the screen to check the status of DSP (digital signal DSP STATUS processor) dynamic parameters. Status updates are in real time. D SP STATUS PR ESSU R E: nnnn PSI TA C H VA LU E: 5.00 V FLO W M O DE: O FF H EAD M ATER IAL: IN ERT...

  • Page 134: Dx-Lan Status

    GP50 Gradient Pump C.2.5 DX-LAN Status Use the screen to monitor several types of errors that may DX-LAN STATUS occur on the network. D X-LAN STATU S U N IQ UE ID : FF FF FF LA N AD D R ESS:...
  • Page 135 C • Display Screens BUS READ Indicates that a ready response could not be issued within 2.4 microseconds after the ready signal was asserted. This occurs when reading an empty buffer. OVERFLOW Indicates that the DX-LAN hardware receive buffer became full and had to reject a packet for lack of space.

  • Page 136: Keyboard Test

    GP50 Gradient Pump C.2.6 Keyboard Test Use the screen to conduct an interactive test of the front KEYBOARD TEST panel keypad buttons. O FF/O N R U N/H O LD LEFT R IG HT PR IM E R ESET D O W N...

  • Page 137: Diagnostic Test

    C • Display Screens C.2.7 Diagnostic Test Use the screen to test the GP50 electronics. DIAGNOSTIC TEST DIAG NO STIC TEST O PEN PR OPO RTIO NIN G VALVE: PO W ER LO OP CO M DSP C OM PU M P LEA K...
  • Page 138 GP50 Gradient Pump To manually run a test, position the cursor in the edit field next to the test Select ∆ Select ∇ name, press to select the asterisk (*), and press . The table below explains the test status indicators.

  • Page 139: Pressure Statistics

    = System is in constant flow mode. = System is in pressure feedback mode. P-POINT LEFT Reports the current pressurization point for the left RIGHT and right pistons. The GP50 updates the readout once per piston stroke. C-23 Doc. 031377-03 7/01...

  • Page 140: Dsp Code Version

    GP50 Gradient Pump C.2.9 DSP Code Version Use the screen to view the current DSP (digital DSP CODE VERSION signal processor) code revision numbers. DS P C O D E VE RSIO N DS P B IO S R EV n.nn...

  • Page 141: Calibration Screens

    Figure C-20. Calibration Menu Screen There are two ways to select a screen from the menu: Press the numeric button on the GP50 front panel keypad that corresponds to the screen number on the menu. For example, press 4 to select and display the screen.

  • Page 142: C.3.2 Calibration Status

    GP50 Gradient Pump C.3.2 Calibration Status Use the screen to check the status of calibration CALIBRATION STATUS parameters in real time. C A LIB R ATIO N STATU S FLO W R ATE C AL: n.nn g PR ESSU R E O FFSET:...

  • Page 143: Leak Sensor Calibration And Status

    C • Display Screens C.3.3 Leak Sensor Calibration and Status Use the screen to check the LEAK SENSOR CALIBRATION AND STATUS status of leak sensor parameters and to calibrate the sensor. column displays the status of the pump's internal leak PUMP sensor.

  • Page 144: Degas Status

    GP50 Gradient Pump C.3.4 Degas Status Use the screen to check the status of the optional vacuum DEGAS STATUS degas pump calibration parameters in real time and to test the degas pump. D EG A S S TAT U S...

  • Page 145: Flow Calibration

    C • Display Screens C.3.5 Flow Calibration Use the screen to calibrate the pump. FLOW CALIBRATION FL O W C A LIB R ATIO N C A LIB R ATE : R D Y PR ES SU R E : nn nn PS I P -P O IN T LEF T:...

  • Page 146: Pressure Calibration

    GP50 Gradient Pump C.3.6 Pressure Calibration Use the screen to calibrate the pump pressure PRESSURE CALIBRATION offset and slope. PR E SS U R E C A LIB R ATIO N O P EN P R ES SU R E TR A N S D U C E R WA S TE VA LV E,...
  • Page 147 C • Display Screens 4. When the pressure reaches zero, select and press Enter OFFSET CAL The offset calibration begins. When calibration is complete, the screen displays the following: P R E S S U R E C A LIB R ATIO N 'E N TE R ' TO U S E S LO P E D E FA U LT VA LU E , O R S E LE C T 'S LO P E C A L' TO C O N T IN U E C A L M O D E :...
  • Page 148 GP50 Gradient Pump Calibrating the Slope After selecting in Step 5, the screen displays the following: SLOPE CAL PR E SS U R E C A LIB R ATIO N C LO S E WA ST E VA LVE , IN STA LL G A U G E .
  • Page 149 C • Display Screens 3. In the field, enter the flow rate normally used for your FLOW application. 4. Turn on the pump. The pressure will begin increasing. Allow the pressure gauge reading to stabilize. 5. Backpressure tubing If backpressure tubing is installed, the recommended pressure to use for calibration is 17.25 ±...

  • Page 150: Degas Pump Calibration

    GP50 Gradient Pump C.3.7 Degas Pump Calibration Use the screen to calibrate the degas pump. DEGAS PUMP CALIBRATION D E G AS P U M P CA LIB R ATIO N C A LIB R ATE D E G AS TH R E SH O LD :...

  • Page 151: D • Ttl And Relay Control

    D • TTL and Relay Control The strip of eight 2-pin connectors on the GP50 electronics chassis provides two relay outputs, two TTL outputs, and four TTL inputs (see Figure D-1). To control functions in another device, connect the GP50 TTL or relay outputs to the TTL or relay inputs of the device to be controlled.

  • Page 152: Ttl And Relay Output Operation

    GP50 Gradient Pump TTL and Relay Output Operation The GP50 provides two TTL outputs and two relay contacts for controlling functions in external devices such as an integrator, autosampler, or another Dionex instrument. After connecting the TTL and Relay outputs (see Section D.3), toggle the output states on and off from the screen (see Section C.1.3) for Direct control, or...

  • Page 153: Ttl Input Operation

    TTL Input Operation The four TTL inputs can be connected to devices capable of providing TTL output signals. Each TTL input controls a specific function in the GP50. A signal from the connected device triggers the function associated with that input.
  • Page 154 The minimum pulse width guaranteed to be detected is 50 ms. The maximum pulse width guaranteed to be ignored as noise or invalid is 4 milliseconds. The action of the GP50 is undefined for pulses less than 50 ms or greater than 4 ms.

  • Page 155: Ttl And Relay Connections

    The signal wire goes on top and the ground wire goes on the bottom of each plug. 2. Connect these plugs to the TTL or relay connectors on the GP50 and the other instruments(s) as needed for your application. Check the polarity of each connection.

  • Page 156: Example Connections

    GP50 Gradient Pump D.3.1 Example Connections Figure D-5 shows an example of TTL/relay connections for a Dionex system connected to an AS40 Automated Sampler. Refer to the AS40 operator's manual for details. Pump Detector RLY-1 RLY-1 RLY-2 RLY-2 TTL-1 TTL-1...
  • Page 157 Figures D-6 and D-7 show two different TTL/relay connections for a Dionex system connected to an AS3500 Autosampler: Setup 1, recommended for isocratic conditions, starts running each Dionex module timed events method when the autosampler makes an injection. Setup 2, recommended for gradient conditions, starts running each Dionex module method before the autosampler makes an injection.
  • Page 158 GP50 Gradient Pump Pump Detector AS3500 RLY-1 RLY-1 RLY-2 RLY-2 TTL-1 TTL-1 TTL-2 TTL-2 Inject Hold Input TTL-1 TTL-1 TTL-2 TTL-2 Hold/Run Hold/Run Gradient Start Output TTL-3 TTL-3 Ground TTL-4 TTL-4 DX-LAN Note: The pump and detector TTL input signal modes must be set to NORMAL PULSE.
  • Page 159 Calibration Status, C-26 AS3500 Autosampler, D-7 Degas Pump Calibration, C-34 AS40 Automated Sampler, D-6 Degas Status, C-28 Automated control of GP50, 1-1, 2-18 Flow Calibration, C-29 Leak Sensor Calibration and Status, C-27 Pressure Calibration, C-30 See also Diagnostic screens Backlight, 2-2, C-11...
  • Page 160 GP50 Gradient Pump Cards Specification, A-3 See Printed circuit boards Summary, 2-19 Cascading hubs, B-9 Control panel, 2-2 CE Mark, 1-4 Keypad, 2-3 Check valves, 3-2 Convex gradient curves, 2-23 Cartridges, 5-3 CPU card, 2-10 Cause of pressure fluctuations, 4-2...
  • Page 161 10BASE-T connections, B-6 Digital Signal Processor (DSP), 1-1, C-17 See also DSP BNC connections, B-9 Dimensions, A-1 Card description, 2-10 Dionex, Contacting, 4-1 Diagnostic test, C-22 Direct control mode, 3-6 Rear panel connector, 2-18 Description, 2-18 DX-LAN Status screen, C-18...
  • Page 162 GP50 Gradient Pump Electronics chassis, 2-8 Vacuum degas fails, 4-10 Connections, B-4 Example methods, 3-13 – 3-24 CPU, 2-10 External leak sensor, C-27 LC leak, 2-9, B-4 LC30 communications, B-5 TTL/Relay, 2-10 TTL/Relay connections, D-5 Facilities required, B-1 Eluent manifold, 2-13 Filter, end-line, 3-1 –...
  • Page 163 Index Gradient curves, 2-22 LC leak, 2-9, B-5 Concave, 2-23 LC10/LC20 connections, B-4 Convex, 2-23 LC25 connections, B-5 Linear, 2-23 LC30 connections, B-5 Gradient eluent run, 2-22 Power connection, B-2 Curved gradient example, 3-19 Priming the manifold, B-17 Linear gradient example, 3-14 Pump interface card, B-6 –...
  • Page 164 GP50 Gradient Pump Adjusting the screen contrast, 2-2 Liquid leaks LC leak, 2-9, B-5 See Leaks LC10 Chromatography Organizer Liquid lines Connections to pump, B-4 PEEK, 5-2 Leak control cable, 2-9 Stainless steel, 5-2 Solenoid valve connections, 2-10 Local mode, 2-19...
  • Page 165 Index Method steps Adding, 2-4 Off/On button, 2-4 Blank fields in, 2-5, 3-8 Offset calibration, C-30 Definition, 2-21 Operating configuration Deleting, 2-5 TTL, D-3 Entering, 3-7, C-6 Operating mode, 2-18 Inserting, 3-9 Direct control, 2-18, 3-6 Maximum number allowed, 2-21 Local mode, 2-18 –...
  • Page 166 GP50 Gradient Pump PeakNet 6 software, 2-18 – 2-19, C-4 Priming block, 2-15 Connect command, 2-20 Priming valve Selecting Locked Remote mode, 2-20 Leaks from, 4-7 PEEK liquid lines, 5-2 Printed circuit boards, 2-8 Periodic maintenance, 3-27 Connectors, 2-9 Physical description...
  • Page 167 Index Screen contrast, 2-2 Screens Rear panel, 2-18, B-3 See Display screens 10BASE-T DX-LAN interface, B-8 Seal replacement, 5-4 BNC DX-LAN interface, B-11 Select buttons, 2-5 Fuses, 5-13 Self-Regenerating Suppressor (SRS) Power receptacle, B-3 Automatic power shutoff, 4-11, B-20 – Waste lines, B-13 B-21 Regulator, 3-2...
  • Page 168 GP50 Gradient Pump TUV, 1-4 Type of control Technical support, 4-1 See Operating mode Temperature, A-1 Tests Diagnostic, C-21 Keyboard, C-20 Pressure transducer, C-23 User interface, C-1 Tilting the front panel, 2-2 User interface screens Time See Display screens Pump, seals, backlight in use, C-16...

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