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Dionex GP40 Operator's Manual

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GP40 GRADIENT PUMP
OPERATOR'S MANUAL
© Dionex Corporation 1995
Document No. 034856
Revision 03
May 1995

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Summary of Contents for Dionex GP40

  • Page 1 GP40 GRADIENT PUMP OPERATOR’S MANUAL © Dionex Corporation 1995 Document No. 034856 Revision 03 May 1995...
  • Page 2 Dionex Corporation, 1228 Titan Way, Sunnyvale, California 94088-3603 U.S.A.

  • Page 3: Table Of Contents

    Contents 1 • Introduction Overview ..... . About This Manual ....1.2.1 Typefaces .
  • Page 4 Contents Rear Panel ..... 2-20 Functional Description ....2-21 2.8.1 Operating Modes .
  • Page 5 Contents 3.4.4 Editing a Running Method Example . . 3-26 Routine Maintenance ....3-29 3.5.1 Daily Maintenance ... . 3-29 3.5.2 Periodic Maintenance .
  • Page 6 Contents Proportioning Valve Replacement ..5-14 Active Mixer Filter Replacement ..5-16 Changing Main Power Fuses ... . 5-19 Doc.
  • Page 7 Contents A • Specifications A.1 Electrical ..... . A.2 Environmental ....A.3 Physical .
  • Page 8 Contents C • User Interface C.1 Operational Screens ....C.1.1 Main Screen ....C.1.2 Detail Screen .

  • Page 9: Introduction

    1 • Introduction Overview ..... . . About This Manual ....1.2.1 Typefaces .
  • Page 10 GP40 Gradient Pump Doc. 034856-03 5/95...

  • Page 11: Overview

    1 • Introduction 1.1 Overview The GP40 Gradient Pump is an integral part of a DX 500 chromatography system. It is a microprocessor-based, dual-piston, variable-speed, gradient delivery system designed to blend and pump mixtures of up to four different mobile phases at precisely controlled flow rates.

  • Page 12: About This Manual

    Chapter 5, Service, presents step-by-step instructions for service and parts replacement routines. Appendix A, Specifications, contains the GP40 specifications and installation site specifications. Appendix B, Installation, describes the installation steps necessary to place the GP40 Gradient Pump into operation.

  • Page 13: Typefaces

    1 • Introduction 1.2.1 Typefaces Typefaces are used in this manual as follows: • Capitalized bold type indicates a front panel button: Press to begin running the method. Enter • Upper-case bold type indicates the name of a screen, the name of a menu, or an on-screen entry: Go to the screen.

  • Page 14: Symbols

    GP40 Gradient Pump Informational messages also appear throughout this manual. These are labeled NOTE and are in bold type: NOTE NOTES call attention to certain information. They alert you to an unexpected result of an action, suggest how to optimize the performance of the instrument, etc.

  • Page 15: Related Manuals

    1 • Introduction 1.3 Related Manuals During installation and operation of the GP40, you may need to refer to one or more of the following manuals (depending on your system) for information about other modules and components included in a DX 500 system.
  • Page 16 GP40 Gradient Pump Doc. 034856-03 5/95...

  • Page 17: Description

    2 • Description Front Control Panel ....2.1.1 Control Panel Keypad ... 2.1.2 Display Screens ....2-10 Electronics Chassis .
  • Page 18 GP40 Gradient Pump Doc. 034856-04 10/95...
  • Page 19 The GP40 is designed to have other single- or dual-unit modules stacked on top of it, to a maximum of four units. See Section B.4 for the recommended stacking configuration for DX 500 systems.

  • Page 20: Front Control Panel

    GP40 Gradient Pump 2.1 Front Control Panel The control panel on the upper door of the GP40 enclosure contains the liquid crystal display (LCD), the membrane keypad, and the actuator for the main power switch (see Figure 2-2). The door opens to provide access to the electronics chassis, described in Section 2.2.

  • Page 21: Control Panel Keypad

    (for tilting the door) the contrast) the panel) Figure 2-2. GP40 Display and Keypad Layout 2.1.1 Control Panel Keypad The keypad is used to directly control pump operation, as well as to create and modify programmed series of timed events, called methods. In summary: •...
  • Page 22 GP40 Gradient Pump predetermined options. Pressing a button increases Select (or decreases) a numerical value by one, while holding down a button increases (or decreases) a numerical Select value continuously. • To execute the selected value, press , or press an...
  • Page 23 2 • Description Insert Inserts a new timed step into a method. This button functions only when the cursor is in a field in the TIME METHOD extension screen. METHOD 1. Move the cursor to the field and press . The Insert TIME new step is added after the cursor position.
  • Page 24 GP40 Gradient Pump Hold/Run Turns the method clock off ( ) and on ( ). This button Hold functions only when the pump is under Method control (see Section 2.8.2). When the method clock is in Hold, pressing , starts Hold/Run the clock.
  • Page 25 • From any calibration screen, pressing displays the Menu . Pressing again returns you to Menu CALIBRATION MENU and then to the DIAGNOSTIC MENU MENU of SCREENS. See Figure C-1 for the GP40 screens and menu structure. Doc. 034856-04 10/95...

  • Page 26: Display Screens

    GP40 Gradient Pump Numeric Buttons Enters numeric values into the current entry field. The numeric buttons are 0 through 9 and the decimal. Enter Saves and/or executes changes made in entry fields. After pressing , the cursor moves back to the left margin of Enter the same field.
  • Page 27 MAIN the percentage of eluents to run, the flow rate, or the method number to run. To access the remaining GP40 screens, press the button Menu to display the (see Figure 2-4). From the...

  • Page 28: Electronics Chassis

    GP40 Gradient Pump 2.2 Electronics Chassis The electronics chassis is located behind the upper door of the GP40 enclosure. The chassis includes several electronic cards (printed circuit boards) that are used to control the GP40. Connectors on the cards also allow communication between the GP40 and other DX 500 modules.

  • Page 29: Lc Leak

    1. LC LEAK When a leak occurs in the LC10 or LC20, it is reported to the GP40. The LC30 Chromatography Oven does not connect to the GP40 connector; it has its own internal leak LC LEAK control electronics.
  • Page 30 GP40 Gradient Pump The CPU logic and Relay I/O cards occupy slot 5 in the card cage. The Relay I/O card rides piggyback on the CPU card and extends over the front of slot 4. The card is short enough to allow the optional DX LAN pump interface card (P/N 044195) to mount behind it in slot 4.

  • Page 31: Mechanical Chassis

    2.3 Mechanical Chassis The mechanical chassis is housed in a pull-out drawer located behind the lower door of the GP40 enclosure. The front of the chassis contains the interior components described in Section 2.4. Other mechanical assemblies are located inside the chassis drawer.

  • Page 32: Pump Eluent Manifold

    GP40 Gradient Pump T o C o l u m n Eluent Eluent Eluent Eluent A In B In C In D In Mixer (microbore only) Bulkhead Pressure Fittings Transducer Manifold and Proportioning P u m p Valves Heads Priming Block...

  • Page 33: Pump Heads

    2 • Description 2.4.2 Pump Heads There are two GP40 pump head configurations: standard bore and microbore. The table below summarizes the features of each type and the operating conditions for each configuration. Pump Piston Pump Flow Rate Column Maximum...

  • Page 34: Pump Mixers

    A pump mixer ensures complete mixing of the proportioned eluents prior to injection. Two types of mixers are available: • In standard bore GP40 pumps (100 µL piston volume), a Dionex active mixer (P/N 048988) is installed between the eluent manifold and the priming block. The active mixer contains an electromagnetic stir bar to mix the proportioned eluents.

  • Page 35: Vacuum Degas Pump Assembly (Optional)

    2.5 Vacuum Degas Pump Assembly (Optional) The Dionex vacuum degas pump (P/N 047263) provides continuous on-line vacuum degassing of one to four eluents (see Figure 2-9). If ordered, the assembly is installed in the pump at the factory. This assembly consists of: •...

  • Page 36: Eluent Reservoirs

    2.6 Eluent Reservoirs Dionex strongly recommends degassing all eluents and storing them in reservoirs pressurized with helium. This helps prevent bubbles (resulting from eluent outgassing) from forming in the eluent proportioning valves, pump heads, and the detector cell.

  • Page 37: Rear Panel

    (Document No. 034581) for installation details. Two optional E01 Eluent Organizers (P/N 044125) fit on top of the system enclosure. Each organizer can accommodate up to two reservoirs with the volume listed below. Contact your Dionex office for ordering information. No. of...

  • Page 38: Functional Description

    2. To change the mode, move the cursor to this field; press Select ∇ to toggle to the desired mode and press or a Enter cursor arrow button. (For TTL input control, set the GP40 to Local mode.) LOAD 100.0 %A 2000 0.0 %B...
  • Page 39 Enter cursor arrow button. NOTE If the GP40 is connected to a host computer, PeakNet commands can also be used to change the operating and control modes. Both Direct and Method control are available in either the Local mode or the Remote mode. The combination of available operating modes and control modes provides flexibility in the way the pump can be operated.

  • Page 40: Operating Modes

    It can be cleared either from the computer or by powering the GP40 down. The GP40 always powers up in the Local mode. If the pump is running a method when you change to the Remote mode, the computer will not interrupt the method unless you send an abort command from the computer.

  • Page 41: Method Control

    The selected eluent mixture is delivered either isocratically, or as a multistep linear or curved gradient. As the method runs, the GP40 calculates the changes in eluent composition required to deliver a gradient from one method step to the next or to match the selected curve.

  • Page 42: Eluent Delivery

    INIT 2.8.3 Eluent Delivery Isocratic Eluent Run The simplest use of the GP40 Gradient Pump is for the delivery of an isocratic (unchanging) mixture of one or more eluents. If more than one eluent is selected, the pump delivers a proportional mixture of the eluents based on the percentage of each eluent selected.
  • Page 43 2 • Description ending points of a gradient step. It is important to note the following points: • The curve number parameter determines whether the pump delivers a linear or curved gradient. Curve numbers are defined as follows: Curve No. Gradient Type 1, 2, 3, 4 Convex...
  • Page 44 GP40 Gradient Pump NOTE A curve number in a step instructs the pump to use the selected curve number when moving from the previous step to that step. Because there are no previous steps for INIT TIME = 0.0 , curve numbers camnot be entered for these steps.

  • Page 45: Operation And Maintenance

    3 • Operation and Maintenance Getting Ready to Run ....3.1.1 Degas Eluents ....Degassing Eluents Manually .
  • Page 46 GP40 Gradient Pump Doc. 034856-03 5/95...

  • Page 47: Getting Ready To Run

    The GP40 with the optional vacuum degas pump assembly provides continuous on-line vacuum degassing of eluents and reagents (four maximum). If the GP40 is not equipped with the vacuum degas assembly, manually vacuum-degas eluents daily, as described below, and store them in pressurized reservoirs.

  • Page 48: Filter Eluents

    GP40 Gradient Pump 5. Install end-line filters and pressurize the reservoirs (see Sections 3.1.2 and 3.1.3). 3.1.2 Filter Eluents Always filter eluents before operation to remove small particulates that may contaminate the proportioning valves or the pump check valves and cause erratic flow rates or loss of prime.

  • Page 49: Start-Up

    2. Press to start the pump flow. Off/On 3. Check the pressure reading on the screen. The GP40 MAIN display updates the pressure readout once per piston stroke. The reading from one stroke to the next should be within 3%.

  • Page 50: Running Under Direct Control

    GP40 Gradient Pump To select the limits: 1. Go to the screen and move the cursor to the DETAIL METHOD field. LIMIT 2. Enter a low pressure limit that is 1.4 to 2 MPa (200-300 psi) below the normal system operating pressure, as indicated by the pressure display on the front panel.
  • Page 51 3 • Operation and Maintenance Figure 3-2. Isocratic Run Profile LOAD 60.0 %A 2000 25.0 %B COLUMN A 2.00 mL/MIN 15.0 %C 0.0 %D LOCAL DIRECT CNTRL Help Message Figure 3-3. Main Screen: Running Under Direct Control 1. Go to the screen.

  • Page 52: Running Under Method Control

    GP40 Gradient Pump Wait at least 10 minutes after starting the pump or changing the flow rate before beginning an analysis. This allows the pump’s real-time electronic pulse damping circuitry to stabilize the flow rate. 3.3 Running Under Method Control This section provides general instructions on how to create, edit, and run methods.

  • Page 53: Creating A New Method

    3 • Operation and Maintenance • For steps other than and time zero, enter a curve number in INIT column. The curve number determines whether the pump delivers a linear or curved gradient (see Section 2.8.3). The default is curve 5 (linear). NOTE A curve number in a step instructs the pump to use the selected curve number when moving from the previous step to that step.
  • Page 54 GP40 Gradient Pump 5. In the field, set the low and high pressure limits LIMITs (see Section 3.1.5). METHOD EDIT SAVE TO R U N 0 LIMITs 0 - 5000 TIME F L O W INIT 100.0 L 1.00 >...

  • Page 55: Running A Method

    3 • Operation and Maintenance 9. After entering the time-based parameters, move the cursor to the field. If you are editing an existing method, SAVE TO enter a new number for the method and press Enter save the method to a new number. If not, press Enter save the current method.
  • Page 56 GP40 Gradient Pump be made when the method clock is stopped, or while it is running. If the method you are editing is currently running, the changes are stored in memory and implemented when you save the method. After you save changes, there is no way to recall the original method.

  • Page 57: Deleting A Method

    3 • Operation and Maintenance event that has already been executed, it will not be incorporated as part of the current run. To run the changed version of the method, press to restart the Reset method at the ial conditions. INIT 3.3.4 Deleting a Method To delete an entire method, move the cursor on the...

  • Page 58: Isocratic Method Example

    GP40 Gradient Pump For all of the method examples, set the pump to Local mode, Method control. To do this, go to the screen, and if MAIN DETAIL necessary, toggle from and from DIRECT CNTRL METHOD REMOTE LOCAL. 3.4.1 Isocratic Method Example Specify an isocratic mixture of 60% eluent A, 25% eluent B, and 15% eluent C to be pumped at 2.0 mL/min.

  • Page 59: Linear Gradient Method Example

    3 • Operation and Maintenance 2. Move the cursor to the field and enter 60; move to the field and enter 25; move to the field and press (15 is automatically filled in to make the eluent Enter percentages equal 100). Skip the (Curve) and (Valve) fields.
  • Page 60 GP40 Gradient Pump • At 30.01 minutes, return to 100% eluent A and re-equilibrate your system for the next analysis. Figure 3-6 illustrates the gradient profile for this method. Figure 3-6. Linear Gradient Method Profile 1. Go to the screen and enter a method number in METHOD field (2, for example).
  • Page 61 3 • Operation and Maintenance METHOD EDIT SAVE TO R U N 0 LIMITs 0 - 5000 TIME F L O W INIT 100.0 L 2.00 > > 0.00 > > Help Message Figure 3-7. Linear Gradient Method Example (After Step 2) 3.
  • Page 62 GP40 Gradient Pump This step marks the end of the second segment and the beginning of the eluent C concentration ramp. Figure 3-8 illustrates the screen as it appears after Step 5. METHOD METHOD EDIT SAVE TO R U N 0...
  • Page 63 3 • Operation and Maintenance step change is a very steep linear gradient in which the eluent composition changes from one eluent to another in 6 seconds. Figure 3-9 illustrates the screen as it METHOD appears after Step 8. METHOD EDIT SAVE TO R U N 0 LIMITs...
  • Page 64 GP40 Gradient Pump METHOD EDIT SAVE TO R U N 0 LIMITs 0 - 5000 TIME F L O W 25.00 100.0 2.00 > > 25.01 100.0 > 30.00 100.0 > 30.01 100.0 Help Message Figure 3-10. Linear Gradient Method Example (Complete) 12.

  • Page 65: Curved Gradient Method Example

    3 • Operation and Maintenance 3.4.3 Curved Gradient Method Example When attempting to optimize the separation of a single component in a complex mixture, it is often helpful to employ paired segments of a curved gradient. For example, if the analyte is an oligonucleotide that elutes with 0.32 M NaCl in a linear gradient run, you can move potentially interfering components of the sample away from the target oligonucleotide by first programming a segment...
  • Page 66 GP40 Gradient Pump Figure 3-12. E1 and E2 Curves The following summarizes the curved gradient method example steps: • Create Method 3 to begin under isocratic conditions with 100% eluent A (25 mM Tris buffer, pH 8.0) at 1.5 mL/min.
  • Page 67 3 • Operation and Maintenance 1. Go to the screen and enter a method number in METHOD field (3, for example). EDIT • If Method 3 currently exists and you want to delete it, move the cursor to and press twice to TIME = INIT Delete...
  • Page 68 GP40 Gradient Pump 5. Move the cursor down to the next line and enter 3.5 in the field. Move the cursor to and enter 68. Move the TIME cursor to and enter 32. 6. Move the cursor to the field and enter 3 to run gradient curve 3.

  • Page 69: Editing A Running Method Example

    3 • Operation and Maintenance 10. Move the cursor to the next line and enter 10 in the TIME field. Move the cursor to and enter 100. After a total of 10 minutes, the concentration of eluent B drops to zero and the concentration of eluent A increases to 100%.
  • Page 70 GP40 Gradient Pump METHOD EDIT SAVE TO R U N 2 LIMITs 0 - 5000 TIME F L O W 10.00 65.0 35.0 2.00 > 15.00 45.0 55.0 > 20.00 40.0 60.0 > 25.00 100.0 > Help Message Figure 3-16. Edited Linear Gradient Method Example: Figure 3-17.
  • Page 71 3 • Operation and Maintenance • If the elapsed time is less than 15 minutes (the time for the first change made to the method), the changes will be incorporated into this run and executed at the programmed time. • If the elapsed time is greater than 15 minutes, the changes will not be incorporated into this run.

  • Page 72: Routine Maintenance

    GP40 Gradient Pump 3.5 Routine Maintenance 3.5.1 Daily Maintenance • When using a combination of eluents which contain both salt or base and solvent, rinse the piston frequently or continuously. Eluent tends to crystallize as the solvent evaporates; these crystals can abrade the piston and cause the main seal to leak.
  • Page 73 4. Dispose of the waste water and close the door to the mechanical chassis. • All components of the vacuum degas assembly are made of inert materials or corrosion-resistant materials. Dionex recommends that you thoroughly flush any chemicals out of the chambers and tubing with deionized water after each use to avoid crystallization in the membrane pores.

  • Page 74: Periodic Maintenance

    3.6 Shutdown • Stop the pump by turning off the main power either on the GP40 or at the power source. Omit this step if you are going to maintain a continuous rinse on the pump heads.
  • Page 75 3 • Operation and Maintenance • If the pump will not be used for three days or more, flush the system with deionized water to prevent contaminants from building up. Or, if this is not possible, maintain a continuous rinse through the system until you resume normal operation. Select a flow rate of 0.04 mL/min for standard pump heads or 0.01 mL/min for microbore pump heads, and set all four valves in the eluent manifold to 25% so that the valves are also flushed.
  • Page 76 GP40 Gradient Pump 3-32 Doc. 034856-03 5/95...

  • Page 77: Troubleshooting

    4 • Troubleshooting Left-Right Pump Head Pressure Fluctuations . . Pump Will Not Start ....Pump Stops ..... Liquid Leaks/Leak Alarm .
  • Page 78 GP40 Gradient Pump Doc. 034856-03 5/95...

  • Page 79: Left-Right Pump Head Pressure Fluctuations

    4 • Troubleshooting This chapter is a guide to troubleshooting common problems that may occur while operating the GP40 Gradient Pump. To use this guide, turn to the section that best describes the operating problem. There, you will find the possible causes of the problem listed in order of probability.
  • Page 80 GP40 Gradient Pump • Pump out of prime; eluent bottles are not pressurized 1. Pressurize the bottles (see Section 3.1.3). 2. Reprime the pump (see Section B.2.7). • Pump out of prime; end-line filter is dirty or clogged 1. Replace the filter (P/N 045987).
  • Page 81 Turn on the pump and observe the pistons. Both pistons should move. If they do not, the rocker arm spring is broken. Notify your Dionex office. Observe the warning label on the inside of the lower door. The arrows on the label indicate moving mechanical parts that present pinch hazards when the pump is on and the mechanical drawer is open.

  • Page 82: Pump Will Not Start

    GP40 Gradient Pump 4.2 Pump Will Not Start • Flow rate is set to zero Reset the flow rate (see Section 2.4.2). • While being primed, pump starts briefly; then stops because of high pressure limit Open the pressure transducer waste valve by turning the knob counterclockwise two turns (see Figure B-5).
  • Page 83 LED on the CPU card inside the door to the electronics chassis. A red LED indicates a defective power supply. Replace the power supply (P/N 046440) (see Figure 2-6). Contact your Dionex office for help. Doc. 034856-03 5/95...
  • Page 84 GP40 Gradient Pump 5. With the pump running, open the screen and note DSP STATUS whether the left-right pressure varies by more than 3% between strokes. If it does, refer to Section 4.1. If it does not, either increase the flow rate or reduce the low pressure limit setting and continue operation.

  • Page 85: Liquid Leaks/Leak Alarm

    4 • Troubleshooting 2. Power up by pressing the power switch. The DSP error message should not reappear; if it does, notify your Dionex office. The power supply (P/N 046440), DSP card (P/N 045369), or CPU card (P/N 046340) may need replacing.
  • Page 86 GP40 Gradient Pump • Leaks from any connection between the eluent reservoir and the pump heads indicates an eluent leak (see Figure 3-19) Tighten the fitting connections just enough to stop the leak. Overtightening the fitting connections may strip the threads in the valve block.

  • Page 87: Noisy Pump Motor

    The DSP handles offset correction but the hardware has no correction. Replace the pressure transducer (P/N 045731). Call your Dionex office for assistance. After replacing the transducer, recalibrate the pressure slope and offset (see Section C.3.6). • Pressure servo oscillation...

  • Page 88: Vacuum Degas Pump Does Not Run

    If the connections are correct, the distribution card (P/N 045714) may need to be replaced. Call your Dionex office for assistance. e. Push the mechanical chassis drawer back in place, making sure the cables are not pinched.

  • Page 89: Vacuum Degas Pump Calibration Fails

    4. Push the mechanical chassis drawer back in place, making sure the cables are not pinched. Retighten the drawer lock. Turn on the power. 5. Retry the calibration. If the message reappears, notify your Dionex office. 4-13 Doc. 034856-03 5/95...

  • Page 90: Inoperative Relay Control Function

    GP40 Gradient Pump 4.8 Vacuum Degas Pump Low Vacuum The GP40 monitors the degas vacuum reading every 1 min. If the degas vacuum is lower than the monitoring value, the degas pump turns on. When the pump turns off, if the vacuum reading...

  • Page 91: Poor Chromatographic Reproducibility

    4 • Troubleshooting 4.10 Poor Chromatographic Reproducibility • Liquid lines incompletely flushed after an eluent change Attach a syringe to the priming block and draw at least 2.5 mL (10 mL if the vacuum degas assembly is not installed) of the new eluent through the liquid lines before beginning operation.
  • Page 92 GP40 Gradient Pump 4-16 Doc. 034856-03 5/95...

  • Page 93: Service

    5 • Service Cleaning the Check Valves ... . . Piston Seal Replacement ....Pump Piston Replacement ... . . 5-10 Pressure Transducer Pad and O-Ring Replacement .
  • Page 94 GP40 Gradient Pump Doc. 034856-03 5/95...

  • Page 95: Cleaning The Check Valves

    5 • Service This chapter describes service and repair procedures for the mechanical components of the GP40. Each procedure specifies the problem it is intended to eliminate. Before replacing any part, refer to the troubleshooting information in Chapter 4 to isolate the cause of the problem.
  • Page 96 GP40 Gradient Pump Pressure Transducer Outlet Check Valve Pump Head To Column To Waste Inlet Check To Eluent Manifold Valve Figure 5-1. Pump Heads and Liquid Lines (PEEK) Pressure Transducer To Column Outlet Check Valve Pump Head To Eluent Inlet Check...
  • Page 97 5 • Service 6. The inlet check valve assembly housing has a 1/4-28 port. Replace the cartridge in the inlet check valve housing; the double-hole end of the cartridge should be visible. The outlet check valve assembly housing has a 10-32 port. Replace the cartridge in the outlet check valve housing;...

  • Page 98: Piston Seal Replacement

    GP40 Gradient Pump 5.2 Piston Seal Replacement A damaged seal allows leakage past the piston and then through the rinse ports in the front of the pump heads. Flow rates will be low and erratic, producing chromatograms with longer than normal retention times.
  • Page 99 5 • Service Piston O-Ring B a c k u p W a s h e r Back-up Seal Outlet Check Valve Piston Seal Piston Guide P u m p H e a d Inlet Check Valve Figure 5-3. Pump Head Assembly Component Standard Standard...
  • Page 100 GP40 Gradient Pump 7. To remove the piston guide and seal: a. Fill the head cavity with deionized water by injecting through either the piston opening or the inlet check valve. b. Reinsert the piston approximately 1/8-in into the seal (see Figure 5-4).
  • Page 101 5 • Service 8. To install the new seal and reinstall the piston guide: a. Push the piston through the piston guide and the new seal. Then insert the piston, piston guide, and seal into the pump head until the seal makes contact with the bottom of the counterbore (see Figure 5-5, View A).

  • Page 102: Pump Piston Replacement

    GP40 Gradient Pump 9. Dionex recommends reinstalling the head and piston as a single assembly so that the piston will center itself. To do this, first press the backup washer into the head until it is flush with the indented surface of the head. Then, insert the piston halfway into the head.

  • Page 103: Pressure Transducer Pad And O-Ring Replacement

    6. If the piston is broken, also replace the piston seal (see Section 5.2) and the backup seal. 7. Dionex recommends reinstalling the head and piston as a single assembly so that the piston will center itself. First press the backup washer into the head until it is flush with the indented surface of the head.
  • Page 104 GP40 Gradient Pump 3. Disconnect the waste line from the pressure transducer housing. 4. Remove the system out line from the pressure transducer housing. 5. Disconnect the transducer connection from the distribution card. To access the card, use a 7-mm open-end wrench or your fingers to loosen the lock on the mechanical chassis drawer.

  • Page 105: Pressure Transducer Waste Valve O-Ring Replacement

    5 • Service 8. Install a new O-ring (P/N 048968) and pressure pad (P/N 048969) in the housing. Reinstall the transducer assembly in the pump. 9. Reconnect the liquid lines from the check valve housings. Reconnect the outlet line and the waste line. 10.

  • Page 106: Proportioning Valve Replacement

    GP40 Gradient Pump 6. Reinstall the valve in the housing, turning the knob clockwise until the valve is seated. Overtightening the valve may damage the seat and pressure transducer housing. 5.6 Proportioning Valve Replacement A defective proportioning valve can cause nonreproducible eluent compositions.
  • Page 107 5 • Service Fastener Proportioning Manifold Valve/Manifold Outlet Assembly Figure 5-7. Location of Proportioning Valve Assembly 5. Loosen the captive fasteners securing the proportioning valve/manifold assembly to the bulkhead (see Figure 5-7). Pull the valve/manifold assembly forward and disconnect the eluent lines exiting the vacuum chamber.

  • Page 108: Active Mixer Filter Replacement

    GP40 Gradient Pump 8. Align the new assembly as shown in Figure 5-7 and mount it to the bulkhead. Tighten the fasteners. 9. Push the mechanical chassis drawer back in place, making sure the cables are not pinched. Retighten the drawer lock.
  • Page 109 5 • Service Pressure Transducer Remove two screws Priming Block/Active Mixer Unit Inlet Check Valve and Tube Fitting Figure 5-8. Location of Priming Block/Active Mixer Unit The priming block and active mixer form a single unit located below the pressure transducer. Remove the two Phillips screws holding the priming block/mixer unit in place (see Figure 5-8).
  • Page 110 GP40 Gradient Pump Priming Block/Mixer Unit Priming Block Coupler (P/N 048235) Filter (P/N 048277) Mixer Body (P/N 047866) Mixer Assembly Electromagnet (P/N 048988) (P/N 918009) Remove screw Bracket to disassemble (P/N 048234) Pressure Bar (P/N 048460) Screw (P/N 045686) Figure 5-9. Active Mixer Assembly 7.

  • Page 111: Changing Main Power Fuses

    1. Turn off the main power. HIGH VOLTAGE—Disconnect the main power cord from its source and also from the rear panel of the GP40. 2. The fuse holder is part of the main power receptacle on the rear panel (see Figure 5-10). A recessed lock is located on each side of the fuse holder.
  • Page 112 GP40 Gradient Pump Figure 5-10. Main Power Fuse Holder 5-20 Doc. 034856-03 5/95...

  • Page 113: A.1 Electrical

    A • Specifications A.1 Electrical ..... . . A.2 Environmental ....A.3 Physical .
  • Page 114 GP40 Gradient Pump Doc. 034856-03 5/95...
  • Page 115 A • Specifications A.1 Electrical Main Power 85 to 270 Vac, 47/63 Hz; 2.5 amps. The GP40 power supply Requirements is main voltage auto-sensing and requires no manual adjustment. Fuse Two 3.15 amp fast-blow IEC127 fuses (P/N 954745) Requirements A.2 Environmental Operating 10 °C to 50 °C (50 °F to 104 °F)

  • Page 116: A.5 Hydraulics

    0. to 35 MPa (0 to 5000 psi) in increments of Limit 0.05 MPa (7.25 psi); trips after a time-out of 0.4 mL for the standard bore GP40 or 0.1 mL for the microbore GP40 Delay Volume Approximately 0.92 mL for standard bore pump heads or 0.72 mL for microbore pump heads, measured from the...

  • Page 117: A.6 Gradient Control

    A • Specifications A.6 Gradient Control Methods Stores up to 100 separate gradient methods (00 through 99), each of which may contain up to 50 separate steps. The actual number of stored methods depends on available memory. Control The keypad is used to select the pump’s operating parameters and to program methods.
  • Page 118 GP40 Gradient Pump Doc. 034856-03 5/95...
  • Page 119 B • Installation B.1 Facility Requirements ....B.2 Installation Instructions ....B.2.1 Power Connection .
  • Page 120 GP40 Gradient Pump Doc. 034856-03 5/95...

  • Page 121: Facility Requirements

    Appendix A, the GP40 installation facility should meet the following requirements: • Install the GP40 on a sturdy table or workbench with at least 7.5 cm (3 in) free space behind the module for connections and ventilation.

  • Page 122: Installation Instructions

    LC30 Ship Kit from the main power receptacle on the GP40 rear panel to one of the four IEC auxiliary receptacles on the rear panel of the LC30. Leave the GP40 power switch on continuously and use the LC30 main power switch to turn the GP40 on and off.

  • Page 123: Electronic Chassis Connections

    LC COMM chassis (see Figure B-2). 2. Route the solenoid valve cable from the rear of the LC30 through the GP40 upper chase or through one of the side slots. Connect the cable to the connector. LC AIR...

  • Page 124: Lc10 Or Lc20 Connections

    (see LC LEAK Figure B-2). 2. Route the solenoid valve cable from the rear of the LC10 or LC20 through the GP40 upper chase or through one of the side slots. Connect the cable to the connector. LC AIR...

  • Page 125: Dx Lan Network Connection (Optional

    B • Installation B.2.3 DX LAN Network Connection (Optional) For the GP40 to communicate with PeakNet software, a pump interface card (P/N 044196) must be installed in the GP40 and the DX LAN cable (P/N 960404) must be connected to the DX LAN connector on the GP40 rear panel.
  • Page 126 GP40 Gradient Pump 6. Reconnect the ribbon cable to the 60-pin connector on the front panel. The header and connector are key-polarized near the center. The ejector latches should be partially open to accept the cable connector. 7. Connect the DX LAN cable (P/N 960405) to the DX LAN connector on the rear panel.

  • Page 127: Waste Lines

    B • Installation B.2.4 Waste Lines The GP40 requires two waste lines; one line from the pressure transducer assembly, and one from the leak drain. These lines are factory-installed and routed to the rear panel. Place the free end of both waste lines into a single waste container positioned below the level of the GP40 to maintain a positive siphon (see Figure B-3).

  • Page 128: Eluent Outlet Line Connection

    GP40 Gradient Pump B.2.5 Eluent Outlet Line Connection Connect the eluent line exiting the pressure transducer to the appropriate injection valve or column in the LC10 Chromatography Organizer, LC20 Chromatography Enclosure, LC30 Chromatography Oven, or other system component. It is important to minimize the volume between the injection valve and detector.

  • Page 129: Priming The Pump

    B • Installation 4. Install spiral wrap (P/N 035906) on the eluent lines to help organize them. Cut the spiral wrap into three pieces, each 15 cm (6 in) long. Gather all the eluent lines and hold them neatly together with one hand. Install one piece of spiral wrap on the eluent lines near the reservoirs, another piece near the middle of the lines, and the third piece near the pump.
  • Page 130 GP40 Gradient Pump Figure B-4. Priming the Eluent Valve Manifold 6. Verify that the eluent is pressurized to 55 KPa (8 psi). 7. Use the syringe to draw liquid from the reservoir, and through the liquid lines, degas pump assembly, and eluent manifold.
  • Page 131 NOTE After priming, check the pressure reading on the MAIN screen. The GP40 display updates the pressure readout once per piston stroke. The reading from one stroke to the next should be within 3%. A variation of more than 3%, indicates the pump is out of prime. If this is the case, go on to the following steps.

  • Page 132: Priming The Pump Heads With Alcohol

    GP40 Gradient Pump Figure B-5. Priming the Pump Heads Priming the Pump Heads with Alcohol 1. Connect a 10 mL syringe (P/N 016387) to the luer adapter on the priming block (see Figure B-5). 2. Open the pressure transducer waste valve by turning the knob counterclockwise about two turns.

  • Page 133: Automatic Srs Power Control

    TTL connections can be used to automatically switch off the power to a Self-Regenerating Suppressor (SRS) when the GP40 flow stops. The GP40 sends a TTL signal to the detector. After 5 minutes, the detector shuts off the SRS power. The 5-minute delay in shutoff allows momentary flow interruptions without disturbing the SRS.
  • Page 134 GP40. (The TTL TTL2 OUT connectors are located behind the upper door). Route the cable through the upper chases of the GP40 and the CD20 or ED40. Plug the other end into on the detector (see Figure B-7).

  • Page 135: Stacking Modules

    B • Installation B.4 Stacking Modules Dionex DX 500 modules are designed to be stacked on top of each other up to a maximum height of four units. The pump is two units high and typically sits on the workbench with a single-unit or double-unit detector module stacked on top of it.

  • Page 136: Securing Modules (Optional

    • The shoe is a gray-colored rubber rectangle with raised edges. It installs under two modules sitting side-by-side on the laboratory bench, for example, the LC30 Chromatography Oven and a GP40 Gradient Pump. • The tie is a small flexible piece of gray-colored rubber tubing with enlarged square ends.

  • Page 137: Installing A Tie

    B • Installation Shoe Figure B-2. Shoe Installation B.5.2 Installing a Tie 1. Align the two modules side-by-side and open their doors. 2. Slide the narrow center portion of the tie into the side slot of one of the modules. 3.
  • Page 138 GP40 Gradient Pump B-20 Doc. 034856-03 5/95...

  • Page 139: C.1 Operational Screens

    C • User Interface C.1 Operational Screens ....C.1.1 Main Screen ....C.1.2 Detail Screen .
  • Page 140 GP40 Gradient Pump Doc. 034856-04 2/96...

  • Page 141: Operational Screens

    C • User Interface This appendix illustrates and describes all of the screens available for display on the front panel of the GP40. There are three categories of screens: operational, diagnostic, and calibration (see Figure C-1): • Operational screens allow you to create, edit, and run methods that control pump operation, and to select default pump parameters.

  • Page 142: Main Screen

    Press the button twice to activate its function. Menu of Screens This menu lists the GP40 user interface screens. It is displayed when you press Menu MENU of SCREENS...
  • Page 143 C • User Interface C.1 Operational Screens Operational screens are used for controlling the GP40 from the keypad. Following power-up, the screen displays (see MAIN Figure C-3). C.1.1 Main Screen screen displays after power-up and a six-second MAIN time-out of the screen.
  • Page 144 GP40 Gradient Pump mL/MIN Displays the eluent flow rate through the pump in milliliters per minute. In Direct control, set the flow rate in this field also. Displays the total elapsed time that the method clock has been running. The value can be changed. When you enter a new elapsed time, the method settings corresponding to the new time go into effect.

  • Page 145: C.1.2 Detail Screen

    C • User Interface C.1.2 Detail Screen This screen provides the information contained in the MAIN screen as well as some additional operational parameters. DETAIL SCREEN TTL1 100.0 %A 2125 TTL2 0.0 %B 2.00 mL/MIN RLY1 0.0 %C INJECT SAMPLE RLY2 0.0 %D COLUMN...

  • Page 146: Method Screen

    GP40 Gradient Pump C.1.3 Method Screen Use this screen to select, edit, and save methods. METHOD EDIT SAVE TO R U N 10 LIMITs 0 - 5000 TIME F L O W INIT 25.0 25.0 25.0 25.0 L 1.00 >...
  • Page 147 C • User Interface %A - %D The percentage of each eluent occurring at the start of the step. The total of all eluents must equal 100%. The gradient curve type ( through ) to use when moving from the previous step to the current step See Section 2.8.3 for details on the curve types.

  • Page 148: Method Extension Screen

    GP40 Gradient Pump C.1.4 Method Extension Screen The method extension screen shows the column selection and TTL/relay output parameters for each step in the method. Use the topmost entries in the same manner as the screen. METHOD To return to the...
  • Page 149 Selects operating options: BY SETTING : The degas pump runs according to the selected degas options. The GP40 monitors the degas vacuum MONITOR: reading every one minute. If the reading falls 500 counts below the calibration threshold value, the degas pump turns on and runs for the cycle duration time.
  • Page 150 GP40 Gradient Pump The GP40 monitors the degas vacuum reading every one minute. If the degas vacuum reading is at or below the alarm threshold value (2000 counts below the calibration threshold value), the following message displays: LOW VACUUM ALARM!!

  • Page 151: Module Setup

    C • User Interface C.1.6 Module Setup Use this screen to adjust the display backlight, and to toggle the button and error tones on or off. Adjustments made in this screen will be the default until new adjustments are made. MODULE SETUP DISPLAY PANEL BACKLIGHT: MEDIUM...

  • Page 152: Pump Options

    GP40 Gradient Pump C.1.7 Pump Options Use this screen to select the type of pump head installed and to set pump operation parameters. This data must be updated after changing to a different pump head type or when the operational requirements change.

  • Page 153: Time Function In

    C • User Interface C.1.8 Time Function In This screen displays the pump functions that can be controlled with TTL input from another device. The MODE field selects the type of input signal the pump will respond to. The signal type can be changed to match the signal of the controlling device.

  • Page 154: C.2 Diagnostic Screens

    GP40 Gradient Pump C.2 Diagnostic Screens To access the pump diagnostics, select the from DIAGNOSTIC MENU . From a diagnostic screen, pressing Menu MENU of SCREENS returns you to the . From the DIAGNOSTIC MENU DIAGNOSTIC MENU pressing returns you to the...

  • Page 155: Power-Up Screen

    C • User Interface C.2.3 Power-Up Screen This is the same power-up screen that displays when you turn on the GP40. When the pump is connected to the DX LAN, the DX LAN ID number also displays. GP40 GRADIENT PUMP...

  • Page 156: Elapsed Time

    GP40 Gradient Pump C.2.4 Elapsed Time This screen reports for how long various GP40 components have been in use. The status of each parameter updates in real time. ELAPSED TIME RESET MODULE ON: nnnnnnnn hours BACKLIGHT: nnnnnnnn hours PUMP CYCLES: nnnnnnnn cycles...

  • Page 157: Dsp Status

    C • User Interface C.2.5 DSP Status This screen reports the status of GP40 DSP (Digital Signal Processor) dynamic parameters. Status updates are in real time. DSP STATUS PRESSURE: nnnn PSI TACH VALUE: 5.00 V FLOW MODE: HEAD MATERIAL: INERT EN.

  • Page 158: Dx Lan Status

    GP40 Gradient Pump C.2.6 DX LAN Status The DX LAN driver monitors several types of errors that may occur on the network. The detector reads the DX LAN error counts and displays them on this screen, along with the DX LAN addresses.
  • Page 159 C • User Interface to seven extra bits at the end of the packet. This is usually caused by a collision or a faulty transceiver. BUS READ Indicates that a ready response could not be issued within 2.4 microseconds after the ready signal was asserted.

  • Page 160: Keyboard Test

    GP40 Gradient Pump C.2.7 Keyboard Test This screen allows you to conduct an interactive test of the front panel keypad buttons. OFF/ON RUN/HOLD LEFT RIGHT PRIME RESET DOWN INSERT SEL UP HELP MENU DELETE SEL DOWN Help Message Figure C-16. Keyboard Test Screen To test the buttons: 1.

  • Page 161: Diagnostic Test

    C • User Interface C.2.8 Diagnostic Test Use this screen to test the pump electronics. At power-up, some of these tests are run automatically. If any tests fail, the screen opens and displays the status of each test run. Additional tests may be run after the screen DIAGNOSTIC TEST is displayed.
  • Page 162 GP40 Gradient Pump To run one of the following tests, position the cursor in the edit field next to the test, press a button to select the Select asterisk ( ), and press . When the test is complete, the...

  • Page 163: Pressure Statistics

    C • User Interface C.2.9 Pressure Statistics This screen monitors the pressure transducer and reports pressure status values. The status values update while the screen is displayed. PRESSURE STATISTICS PRESSURE: nnnnn.nn PSI FLOW MODE: MAX: nnnnn.nn PSI P-POINT LEFT: nnnnn.nn PSI MIN: RIGHT: nnnnn.nn...

  • Page 164: Dsp Code Version

    P-POINT LEFT Reports the current pressurization point for the left RIGHT and right pistons. The GP40 updates the readout once per piston stroke. The pressure points should not vary more than 3% from one stroke to the next. C.2.10 DSP Code Version Displays the current digital signal processor (DSP) code revision numbers.

  • Page 165: Calibration Screens

    C • User Interface C.3 Calibration Screens To access the pump calibration screens, select the DIAGNOSTIC MENU from the and select option to go to the MENU of SCREENS, CALIBRATION MENU C.3.1 Calibration Menu CALIBRATION MENU CALIBRATION STATUS LEAK CAL & STATUS DEGAS STATUS FLOW CALIBRATION PRESSURE CALIBRATION...

  • Page 166: Calibration Status

    GP40 Gradient Pump C.3.2 Calibration Status This screen reports the status of calibration parameters in real time. CALIBRATION STATUS FLOW RATE CAL: n.nn g PRESSURE OFFSET: nnnn PSI C-E CAL VALUE: Help Message Figure C-21. Calibration Status Screen FLOW RATE The current flow rate calibration value.

  • Page 167: Leak Sensor Calibration And Status

    C • User Interface C.3.3 Leak Sensor Calibration and Status This screen reports the status of leak sensor parameters. The column displays the status of the pump’s internal leak PUMP sensor. The column displays the status of the leak EXTERNAL sensor in the LC10 Chromatography Organizer or the LC20 Chromatography Enclosure.

  • Page 168: Degas Status

    GP40 Gradient Pump C.3.4 Degas Status This screen reports the status of the vacuum degas pump calibration parameters in real time and allows testing of the degas pump. DEGAS STATUS DEGAS READING: nnnnn STATUS: ALARM THRESHOLD: nnnnn TEST: Help Message Figure C-23.

  • Page 169: Flow Calibration

    C • User Interface C.3.5 Flow Calibration This screen reports the pump flow rate calibration and provides instructions for calibration. FLOW CALIBRATION CALIBRATE: PRESSURE: nnnn P-POINT LEFT: nnnn RIGHT: nnnn PUMP DI WATER AT 2000 (+/- 300 ) PSI. ALLOW PUMP TO STABILIZE. COLLECT WATER INTO A TARED BEAKER FOR 5 MINUTES.

  • Page 170: Pressure Offset Calibration

    GP40 Gradient Pump C.3.6 Pressure Calibration Use this screen to calibrate the pump pressure offset and slope. NOTE Before beginning the calibration procedure, make sure that the pump is primed. PRESSURE CALIBRATION OPEN PRESSURE TRANSDUCER WASTE VALVE, THEN SELECT 'OFFSET CAL' AND ENTER...
  • Page 171 C • User Interface To calibrate: 1. Stop the pump flow. 2. Go to the screen. PRESSURE CALIBRATION 3. Open the pressure transducer waste valve by turning it counterclockwise two turns (see Figure 2-7). 4. When the pressure reaches zero, select OFFSET CAL press .
  • Page 172 (see Figure C-28). A pressure gauge assembly (P/N 046175), which includes a gauge, backpressure tubing, and required fittings, is available for this purpose. Contact your Dionex office for details. Pressure Test Gauge Backpressure...
  • Page 173 C • User Interface 8. In the field, enter the flow rate normally used for FLOW your application. 9. Turn on the pump. The pressure will begin increasing. Allow the pressure gauge reading to stabilize. 10. This step depends on whether backpressure tubing or a column is installed: •...

  • Page 174: Degas Pump Calibration

    GP40 Gradient Pump C.3.7 Degas Pump Calibration This screen allows calibration of the degas pump. DEGAS PUMP CALIBRATION CALIBRATE DEGAS THRESHOLD: DEGAS PRESSURE READING: nnnnn DEGAS CALIBRATION VALUE: nnnnn Help Message Figure C-29. Degas Pump Calibration Screen CALIBRATE To calibrate, select...
  • Page 175 D • Pump Head Conversion D.1 Cleaning the Original Pump Head Assembly . . . D.2 Removing the Pump Head Assembly ..D.3 Installing the New Pump Head Assembly ..D.4 Replacing the Mixer .
  • Page 176 GP40 Gradient Pump Doc. 034856-03 5/95...
  • Page 177 D • Pump Head Conversion The GP40 Gradient Pump is available with either a 100 µL piston volume (standard bore) or 25 µL piston volume (microbore) pump head assembly. Kits for converting to standard bore or microbore pump heads are available for PEEK and stainless steel versions. If required by an application, remove the original pump head assembly and install a different version.
  • Page 178 GP40 Gradient Pump Pressure Transducer Inlet Outlet Check Valve Pressure Transducer Pump Head Waste Valve To Column To Waste Inlet Check To Eluent Manifold Valve Figure D-1. Pump Heads and Liquid Lines 4. Open the screen and select MAIN LOCAL DIRECT CNTRL 5.
  • Page 179 D • Pump Head Conversion D.2 Removing the Pump Head Assembly NOTE The pump heads can be removed in any order. 1. Turn off the main power switch. 2. Disconnect the tube fittings from the pressure transducer inlet and from the pump head inlet check valve (see Figure D-1). 3.
  • Page 180 GP40 Gradient Pump D.3 Installing the New Pump Head Assembly NOTE The pump heads can be installed in any order. Refer to Figure D-2 for assembly order of pump head components. 1. Unpack the new pump head assembly. a. Remove the green sleeve caps (P/N 036570) and the yellow caps (P/N 036571) from the check valves on the new pump head assembly.
  • Page 181 D • Pump Head Conversion Piston O-Ring B a c k u p W a s h e r Back-up Seal Outlet Check Valve Piston Seal Piston Guide P u m p H e a d Inlet Check Valve Figure D-2. Pump Head Assembly Component Standard Microbore/...
  • Page 182 GP40 Gradient Pump a. Push the piston through the piston guide and the seal. Then insert the piston, piston guide, and seal into the pump head until the seal makes contact with the bottom of the counterbore (see Figure D-3, View A).
  • Page 183 D • Pump Head Conversion View A Seal Piston G uide Piston Pump Head Push the piston through the seal and guide and partially insert into the head, just until the seal contacts the counterbore. Counterbore View B Remove the piston and push the piston guide into the head to finish seating the seal.
  • Page 184 GP40 Gradient Pump 8. Reconnect both pressure transducer inlet fittings. Tighten the fittings fingertight, then an additional one-eighth turn (2 in-lb). Tighten further only if leaks occur. DO NOT OVERTIGHTEN. D-10 Doc. 034856-03 5/95...
  • Page 185 D • Pump Head Conversion D.4 Replacing the Mixer The type of mixer required by the GP40 depends on which pump head is installed: • Microbore PEEK pumps, require a GM-4 gradient mixer (P/N 049135) to be installed between the pump outlet and the injection valve.
  • Page 186 GP40 Gradient Pump Pressure Transducer Phillips Screws Priming Block/Active Mixer Unit Inlet Check Active Mixer/ Valve and Priming Proportioning Tube Fitting Block Inlet Mixer Bracket Valve Outlet Screw Figure D-4. Location of Priming Block/Active Mixer Unit 4. Locate the lock on the mechanical chassis drawer. It is a bolt on the lower right side of the chassis, between valves 3 and 4.
  • Page 187 (2 in-lb). Tighten further only if leaks occur. DO NOT OVERTIGHTEN. 12. The GM-4 gradient mixer installs between the eluent outlet of the GP40 pressure transducer and the inlet of the injection valve (see Figure D-5). Disconnect the tube fittings from the pressure transducer outlet and the injection valve inlet.
  • Page 188 13. Locate the 4-in piece of 0.125-mm (0.005-in) ID PEEK tubing (P/N 049427) in the kit. Connect one end of the tubing to the GM-4 mixer and connect the other end to the pressure transducer outlet. Use Dionex 10-32 ferrules (P/N 043276) and fittings (P/N 043275) for the connections.
  • Page 189 042690) in the Ship Kit and connect it between the pressure transducer outlet and the injection valve inlet (see Figure D-6). Use Dionex 10-32 ferrules (P/N 043276) and fittings (P/N 043275) for the connections. 3. Use a 1/2-in wrench to loosen the two pump head inlet check valves (see Figure D-7).
  • Page 190 GP40 Gradient Pump Pressure Transducer Phillips Screws Priming Block Priming Block Inlet Location (fitting not shown in drawing) Inlet Check Valve and Proportioning Tube Fitting Valve Outlet Figure D-7. Location of Priming Block 5. Remove the two Phillips screws holding the priming block in place (see Figure D-7).
  • Page 191 D • Pump Head Conversion 9. Attach the new active mixer/priming block unit to the mechanical chassis, using the Phillips screws. Figure D-4 shows an installed active mixer/priming block unit. 10. Push the mechanical chassis drawer back in place, making sure the cables are not pinched.
  • Page 192 GP40 Gradient Pump D.5 Completing the Installation 1. Reconnect the main power cord to the rear panel of the GP40. Turn on the power switch. 2. After replacing a pump head, it is essential to enter the new pump data into the microprocessor so that it can adjust the flow rate display and DSP Moduleware parameters.
  • Page 193 D • Pump Head Conversion 4. Prime the new eluent line from the proportioning valves to the priming block inlet: a. Open the screen (press ) and select Menu Enter MAIN LOCAL DIRECT CNTRL b. Make sure that the button is in the on position. Off/On c.
  • Page 194 GP40 Gradient Pump 6. Calibrate the flow with the new pump heads. To do this, press , and to open the screen (see Menu FLOW CALIBRATION Figure D-9). Select and press . Follow the directions on Enter the screen to calibrate the flow.
  • Page 195 D • Pump Head Conversion D-21 Doc. 034856-03 5/95...
  • Page 196 E • TTL and Relay Control TTL and Relay Output Operation ..TTL Input Operation ....E.2.1 TTL Input Signal Modes ..TTL and Relay Connections .
  • Page 197 GP40 Gradient Pump Doc. 034856-03 5/95...
  • Page 198 TTL outputs, and four TTL inputs (see Figure E-1). • Connect the outputs to the TTL or relay inputs of a Dionex or non-Dionex device to control functions in the connected device. The devices must be compatible with the GP40’s TTL and relay signals.
  • Page 199 GP40 Gradient Pump E.1 TTL and Relay Output Operation The GP40 provides two TTL outputs and two relay contacts to control functions in external devices such as an integrator, autosampler, or other DX 500 module. After connecting the TTL and Relay outputs (see Section E.3),...
  • Page 200 TTL Input 3 above, except that method numbers decrease instead of increase. E.2.1 TTL Input Signal Modes The GP40 TTL inputs respond to four types of signals to accommodate different types of device output signals. The default signal mode, normal edge, is compatible with the output signals provided by DX 500 modules.
  • Page 201 50 ms. The maximum pulse width guaranteed to be ignored as noise or invalid is 4 milliseconds. The action of the GP40 is undefined for pulses less than 50 ms or greater than 4 ms. • Inverted Pulse: The inverted pulse mode operates identically to the normal pulse mode except that the positive and negative edges are reversed in function.
  • Page 202 The signal wire goes on top and the ground wire goes on the bottom of each plug. 2. Connect these plugs to the TTL or relay connectors on the GP40 and the other module(s) as needed for your application. Check the polarity of each connection.
  • Page 203 GP40 Gradient Pump E.3.1 Example Connections Figure E-5 shows an example of TTL/relay connections for a DX 500 system connected to an AS40 Automated Sampler. Refer to the AS40 operator’s manual for details. Pump Detector RLY-1 RLY-1 RLY-2 RLY-2 TTL-1...
  • Page 204 • Setup 2, recommended for gradient conditions, starts running each DX 500 module method before the autosampler makes an injection. The method then controls when the autosampler injection occurs. Refer to the AS3500 manual for details. AD20 CD20 GP40 ED40 IP20 AS3500 RLY-1 RLY-1 RLY-2...
  • Page 205 GP40 Gradient Pump Pump Detector AS3500 RLY-1 RLY-1 RLY-2 RLY-2 TTL-1 TTL-1 TTL-2 TTL-2 Inject Hold Input TTL-1 TTL-1 TTL-2 TTL-2 Hold/Run Hold/Run Gradient Start Output TTL-3 TTL-3 Ground TTL-4 TTL-4 DX-LAN Note: The pump and Note: The pump and detector...
  • Page 206 E • TTL and Relay Control Figure E-8 shows an example of TTL/relay connections for an LC30 Chromatography Oven. Refer to the LC30 manual for details. LC30 AD20 CD20 GP40 ED40 IP20 FAULT RLY-1 RLY-1 O U T E R R O R...
  • Page 207 GP40 Gradient Pump E-12 Doc. 034856-03 5/95...
  • Page 208 Index %A - %D, C-5, C-9 C (curve) column in Method screen, 3-9, C-9 Entering eluent percentages, 3-9 Cable 60-pin ribbon, 2-13 0 Flow, C-12 LC10/20 leak sensor, B-6 100µL pump heads, 2-16, C-12 RJ-11 serial interface, B-5 see also Pump heads Calibration Menu, C-25 25µL pump heads, 2-16, C-12 see also Diagnostic screens...
  • Page 209 GP40 Gradient Pump Selecting, 2-22 DSP Status, C-17 Control panel, 2-4 DX LAN Status, C-18 Keypad, 2-5 Elapsed Time, C-16 Controlling the method clock, 3-14 Hexadecimal entry fields in, C-14 Conventions, 1-5 Keyboard Test, C-20 Convex gradient curves, 2-26 Leak Status, C-27...
  • Page 210 Index E01 Eluent Organizers, 2-20 Facilities required, B-3 Edge input signal mode, E-6 Editing a method, 3-12 Filter, end-line, 3-4, B-3 Editing a running method, 3-26 Fittings Elapsed Time screen, C-16 Bulkhead, 2-15 Electrical specifications, A-3 Flow Calibration screen, C-29 Electronics chassis, 2-11 Flow column, in Method screen, 3-9 Connections, B-5...
  • Page 211 GP40 Gradient Pump Heads Key press sound, C-11 see Pump heads Keyboard Test screen, C-20 Keypad buttons, 2-5 Help button, 2-9 Cursor arrows, 2-8 Hexadecimal entry fields, C-14 Delete, 2-7 Hold/Run button, 2-8 Enter, 2-9 Host computer, 2-23 Help, 2-9...
  • Page 212 Index Linear gradient example, 3-16 Number allowed, 2-25 Running a method, 3-12 Main status screen, C-5 Microbore heads, 2-16 Maintenance see also Pump heads Daily, 3-29 MIN, C-6 Periodic, 3-31 Mixers, 2-17, D-10 Replacing active mixer filter, 3-31 mL/MIN, C-6 Replacing piston seals, 3-31 Modes of operation Rinsing the pump, 3-29...
  • Page 213 GP40 Gradient Pump Linear gradient example, 3-16 Replacing the pad and O-ring, 5-11 Method control, 3-9 Waste valve, B-13 Preparing to run, 3-3 Pressure units, C-12 Priming, B-11, B-13 Pressurization points, C-24 Programming the pump, 3-14 Prime button, 2-6 Running a method, 3-12...
  • Page 214 Index Static mixer, 2-17, D-10 Step gradient change, 2-27, 3-19 System outlet line, B-10 Safety messages, 1-5 Save to field Method screen, C-8 Schematic, eluent flow, 2-15 Screen brightness, C-11 Screen contrast, 2-4 Temperature, A-3 Screens Tests see Display screens Diagnostic, C-21 Seal replacement, 5-6 Keyboard, C-20...
  • Page 215 GP40 Gradient Pump Column switching, C-5, C-10 Injection, C-5 Proportioning, 2-15 User interface, C-3 Testing proportioning valves, C-21 Power-Up screen, C-15 User interface screens see Display screens Waste line installation, B-9 Waste valve, B-13 Weight, A-3 V (valve) column in Method screen, 3-9, C-9...

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