INTRODUCTION Monochromatic rays from Infrared LED with 589.44nm wavelength travels through condenser, lens, polarizer, modulator and collinator to form a plane polarized light. The plane of polarization caused by the alternating current which flows through the Faraday coil at a particular frequency. This polarized light then passes through the sample tube containing the solution to be measured and then through the analyzer, lens, filter and an aperture before falling on the photomultiplier detector.
OPERATIONS Polarimeter should be placed on a stable, vibration free surface in a dry & clean room with good ventilation & temperature around 20 . Power supply to the instrument is 230V and should be sourced through voltage stabilizer 1KVA with a proper earthing.
: Optical rotation of a substance bar : Average optical rotation [] : Specific Rotation [] bar: Average Specific Rotation C : Concentration in gms/100ml C bar : Average concentration Z : Sugar Scale Z bar: Average sugar scale ...
MEASUREMENT Keep the sample tube containing distilled water or any other blank solvent into the sample chamber and close the cover. Press ZERO key to display reading equal to '0'. Make sure that there are no air bubbles in the sample tube by tilting the tube to make the air bubbles float on the protruded tube neck.