BTX Gemini X2 User Manual
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BTX Gemini X2 User Manual

Twin waveform electroporation systems; gemini series
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Gemini Series
Twin Waveform
Electroporation Systems
User's Manual
w w w .b t x o nl in e . c o m
5507-002 REV 1.0

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Summary of Contents for BTX Gemini X2

  • Page 1 Gemini Series Twin Waveform Electroporation Systems User’s Manual w w w .b t x o nl in e . c o m 5507-002 REV 1.0...

  • Page 2: Safety Information

    3 being applied. meters. If there are any questions about the operation of this instrument, call BTX Customer service Observe All Terminal Ratings at 1-800-272-2775, or 1-508-893-8999. Review the operating manual to learn the ratings on all connections.

  • Page 3: Introduction

    Thank you for investing in a Gemini System. Since its founding in 1983, the main focus of BTX has been in the area of applying controlled electric fields for genetic engineering applications. Because of this, we quickly established a reputation as the technological leader in the fields of electroporation and electrofusion.

  • Page 4: Table Of Contents

    Table of Contents Safety Information ........................2 Introduction ..........................3 Table of Contents ........................4 Product Overview ........................5 Electroporation Overview ......................6 General Optimization Guide for Electroporation ..............7-8 Wave Forms ............................. 7 Field Strength ........................... 7 Pulse Length ............................. 8 Number of Pulses ..........................8 Electroporation Buffer ........................

  • Page 5: Product Overview

    The BTX Gemini SC boasts a wide range of all forms with one easy to use setup. The BTX Gemini X2, which...

  • Page 6: Electroporation Overview

    The effects of heating can be reduced protocols for a particular application has usually been achieved by using a low conductivity medium such as BTX’s Cytoporation empirically, by adjusting pulse parameters (amplitude, duration, medium to resuspend cells prior to electroporation.

  • Page 7: General Optimization Guide For Electroporation

    General Optimization Guide for Electroporation As described, electroporation is the application of controlled Cell Type Field Strength Ranges direct current (DC) electrical pulses which are applied to living Bacteria/Yeast: 3-24 kV/cm cells and tissues for a short duration of time. The pulse induces a Mammalian: 0.25-3 kV/cm transmembrane potential which causes the reversible breakdown Plant: 3-12 kV/cm...

  • Page 8: Pulse Length

    “time constant” which is characterized by the rate at which the pulsed energy (e) or voltage is decayed to 1/3 the BTX now offers BTXpress High Performance Electroporation original set voltage. This time constant is modified by adjusting...

  • Page 9: Applications

    Molecular and Cellular researchers working with either of these two species. This is why Neuroscience, 44, 43 -54, 2010 researchers consistently turn to BTX for all of their electroporation needs. Yao, S. et. al., Improvement of electroporation to deliver plasmid DNA into dental follicle cells.

  • Page 10: General References

    Applications General References (continued) Benton, C. et al., Modest PGC-1_ Overexpression in Muscle in Vivo Is Sufficient to Increase Insulin Sensitivity and Palmitate Oxidation in In Vitro Electroporation (continued) Subsarcolemmal, Not Intermyofibrillar, Mitochondria*. The Journal of Biological Chemistry, 283(7); pp. 4228–4240, February 15, 2008 Zhao, Y.
  • Page 11 Applications General References (continued) Ex Vivo Electroporation (continued) Deora, A.A. et. al., Efficient Electroporation of DNA and Protein into Confluent and Differentiated Epithelial Cells in Culture. Traffic, 8: 1304-1312, 2007 Thomas J-L. et. al., Electroporation, an alternative to biolostics for transfection of Bombyx mori embryos and larval tissues.

  • Page 12: Glossary Of Terms

    Specialty Electrodes – Electrodes used with the BTX Gemini Electrolytic – A fluid containing charged molecules is called an X2 to perform electroporation on a wide variety of cell forms.

  • Page 13: Electroporation Buffers

    “hard to transfect” by chemical and other non-viral methods. This solution, in combination with BTX electroporators, provides researchers with the versatility needed for success across a broad range of cell types while maintaining critical cell viability.

  • Page 14: Unpacking The System

    Gemini SC Power up the system by pushing the rocker switch located on the back panel at the bottom left. The display will flash the BTX logo. Once the software initializes, the Main Menu screen will appear. You are now ready to begin your work.

  • Page 15: Touchscreen Button Reference

    Touchscreen Button Reference Accept Stop Protocol Used to accept the parameters/settings on a screen and Used during the pulse delivery sequence to stop the advance to the next screen in the menu, also used in progress of the protocol. place of a double tap on various icons. Square Wave Indicator Back Used to indicate when a protocol utilizes square...

  • Page 16: Software Setup

    Software Setup Setting Time/Date Setting Audible Alarm Preferences After initializing, the home screen will appear. On the home screen, tap the Gears icon Tap the Gears icon Tap Audible Alarms icon. Tap Time/Date icon. Select preferred audible alarms by tapping the icons to activate or deactivate.

  • Page 17: Setting Backlight Preferences

    Software Setup Displaying Device Information Setting Backlight Preferences On the home screen, tap the Gears icon. On the home screen, tap the Gears icon Tap Backlight icon. Tap Device Information icon. Select preferred brightness by tapping the icons indicating View device information. % brightness.

  • Page 18: Preset Protocols

    Once protocol is complete, data regarding your pulse is displayed Double tap desired cells. and stored in the systems logs (BTX Gemini X2 only) for future use. You may continue pulsing, go back to the protocol select screen, or to the home screen.

  • Page 19: Customizing A Preset Protocol

    Preset Protocols Customizing a Preset Protocol (continued) Customizing a Preset Protocol Use keyboard to name copy of protocol. On the home screen, tap the Preset Protocols icon. Tap the Green Check icon to save. Double tap desired cell type. The message board will turn green and alert the user that the method has been copied.

  • Page 20: Preset Protocols

    Preset Protocols Customizing a Preset Protocol (continued) Scroll through user protocols to locate the newly saved method. Double tap the newly saved method. Tap the parameter(s) requiring customization. Proceed to modify selected parameters. Tap the Green Check icon to proceed to the run screen. Gemini Series Electroporator User’s Manual toll free 800.272.2775 •...

  • Page 21: Performing Experiments

    Performing Experiments Creating New Protocols Creating an Exponential Decay Wave Protocol Tap the Exponential icon. On the home screen, tap User Protocols icon. Tap the Green Check icon to save. Double tap New Protocol. Double tap your newly saved protocol. Use keyboard to name protocol.
  • Page 22 Use the numeric keypad to set the # of pulses. ✐ Tap the Resistance (ohms) icon. NOTE: Only the BTX Gemini X2 can perform multiple pulsing with Exponential Decay Wave, the BTX Gemini SC cannot Use the numeric keypad to set resistance value.

  • Page 23: Creating An Exponential Decay Wave Protocol

    Tap the Green Check icon to save. Once protocol is complete, data regarding your pulse is displayed and stored in the systems logs (BTX Gemini X only) for future use. You may continue pulsing, go back to set-up screen, or to the Tap the Green Check icon to proceed to the run screen.

  • Page 24: Creating A Square Wave Protocol

    Performing Experiments Use the numeric keypad to set voltage Creating New Protocols (continued) Tap the Green Check icon to save. Creating a Square Wave Protocol Tap the Square Wave icon. Tap the Green Check icon to save. Tap the Duration icon. Double tap your newly saved protocol.
  • Page 25 Performing Experiments Tap the Gap icon Creating New Protocols (continued) Creating a Square Wave Protocol (continued) Tap the Pulses Interval icon. Use the numeric keypad to set the gap between electrodes. Tap the Green Check icon to save. Tap the # Pulses icon. Use the numeric keypad to set the # of pulses.
  • Page 26 With load measurement OK, press the Go icon to run protocol. Once protocol is complete, data regarding your pulse is displayed and stored in the systems logs (BTX Gemini X2 only) for future use. You may continue pulsing, go back to the set-up screen, or to the home screen.

  • Page 27: Using Specialty Protocols

    Using Specialty Protocols Using an HT Plate Handler, Gemini X2 only Plug the HT Plate Handler into the front ports of the BTX Gemini X2. Scroll through available cells. Double tap desired cells. Review parameters. Set the HT plate handler with the correct number of pre-pulse resistance measurement and DC pulses.

  • Page 28: Using An Ht Plate Handler

    Using Specialty Protocols Using an HT Plate Handler, Gemini X2 only Review parameters. (continued) Position electrodes on area being electroporated. Once protocol is complete, data regarding your pulse is displayed To run tap the Omega icon. and stored in the systems logs for future use.
  • Page 29 Using Speciality Protocols Using Specialty Electrodes, Gemini X2 Only Once protocol is complete, data regarding your pulse is displayed and stored in the systems logs for future use. You may continue pulsing, go back to the settings screen or the protocol select screen, or to the home screen.

  • Page 30: Managing Protocols

    Protocols to keep your Protocol List easy to navigate. protocol: In addition, the BTX Gemini X2 allows you to export Protocol 1. From the Protocol Configuration screen, choose the information to an externally connected generator or to a computer Protocol Name button to enter the Protocol Mode screen.

  • Page 31: Deleting A Protocol

    Managing Protocols Deleting a Protocol Confirm delete by tapping the Delete icon again. ✐ NOTE: Preset protocols cannot be deleted. On the home screen, tap the User Protocols icon. Protocol has been removed from the menu. Tap the protocol you wish to delete. Tap Folder icon.

  • Page 32: Password Protecting A Protocol

    Managing Protocols Password Protecting a Protocol Confirm password protection by tapping the Open Lock icon again. On the home screen, tap the User Protocols icon. Use the numeric keypad to set the password. Double tap protocol you wish to password protect. Tap the Green Check icon to save.

  • Page 33: Protocol Manager Software

    4. Select the proper COM port. Overview Protocol Manager allows the user to: • Upload protocols from the Gemini X2 generator to a PC. • Download protocols from PC to the Gemini X2 generator. • Upload log files from the Gemini X2 generator to PC.

  • Page 34: Download- Pc To Generator

    Protocol Manager Software ✐ NOTE: If a protocol already exists on the Gemini X2 generator, Protocol Manager will not overwrite the file if you attempt to re-download it. 4. Protocol name being downloaded is shown Gemini Series Electroporator User’s Manual...

  • Page 35: Upload Log Files

    Protocol Manager Software ✐ Upload Log Files NOTE: Log Files are saved as a tab delimited text file. They 1. On the main screen, click ‘Pulse Data’ can be opened with any common text editor like Notepad or by 2. Select your desired files or folders and then click ‘Transfer’. using spreadsheet software such as Excel.

  • Page 36: Remote Control Software

    Control Panel and select System and Security, and then System. Click Device Manager on the left hand menu. Under Ports, search for the “USB serial port for BTX Gemini.” This will show the COM Port to which the generator is connected, as shown below.

  • Page 37: Generator Specifications

    Generator Specifications Generator Specifications Gemini X2 Gemini SC Square Wave Pulse Ranges Voltage Range LV Mode 5-500 in 1 V steps 10-500 V in 5 V steps HV Mode 505-3000 in 5 V steps 510-3000 in 10 V steps Voltage Accuracy...

  • Page 38: Generator Specifications

    Generator Specifications Generator Specifications (V7) Gemini X2 Gemini SC Other Spcifications (continued) Interlock Switches Pre-Pulse Sample resistance check Pulse Over Current Protection PC control PC communications Log Report Remote Operation Foot Switch Control Power Ratings 35 W idle and 350 W pulsing...

  • Page 39: Maintenance

    Maintenance The BTX Gemini Twin Wave Series generators require no special Battery Replacement maintenance other than keeping them clean. 1. Remove screw and swing cover to the side. To clean the exterior surfaces, use a lint-free cloth to remove loose 2.

  • Page 40: Upgrading Gemini Series Software

    2. Two Flash update applications will be displayed in the device manager, under Other Devices. Right click on either of the When you connect the BTX Gemini X2 to a computer via USB flash update applications and select Update Driver for the first time, Windows will seek to install a driver for Software.
  • Page 41 4. Click Browse and navigate to the generator drivers folder located on the CD supplied with the generator. Select the file BTX USB-CDC.inf. Make sure the Include Subfolders box is checked and then click Next. 5. If a warning message regarding the driver publisher is displayed, choose Install this driver software anyway.

  • Page 42: Device Updater Program

    2. Disconnect all I/O devices and then connect the Gemini X2 generator to PC using a USB cable. 3. On the Set up screen of the Gemini X2 generator, press the Upgrade Software button twice to enter Boot Loader Mode.

  • Page 43: Error Messages & Troubleshooting

    Error Messages & Troubleshooting Display Error Message Error Description User Troubleshooting Power Loss Error Power loss during pulsing. Power lost while protocol was running. PFC Over Temp Pfc module over temperature. Hardware error. Please contact the manufacturer. Interlock Open Electrode interlock open. Connect electrode connectors to the front of the generator and make sure the cuvett safety dome cover is closed.

  • Page 44: Troubleshooting

    Troubleshooting ✐ NOTE: It is recommended that you save your protocols periodically by uploading them to a PC. Out of Range A value was entered or encountered in a protocol that was beyond the generator’s limits. Power Failure Notification If power is interrupted during operation, an alarm will sound to alert the user to the interruption.
  • Page 45 Experiment Troubleshooting Arcing Verify electrical component functionality. Verify properties of cell sample (do cells need to be washed? Is the buffer appropriate for application?). Verify properties of transfectant molecule (Is the DNA well purified?) Try reducing the voltage or increase sample volume until arcing is no longer a problem.

  • Page 46: Ordering Information

    BTX Gemini X2 HT Electroporation System. In- 1 ea 5012-017 Pliers for Plate Handler Removing Pins 1 ea cludes Gemini X2 Generator, Cuvettes - 610, 620, 4500-012 25 Well Plate Seal 2 ea 640 pack of 30 (10 ea), Safety Dome 2, HT 200...

  • Page 47: Ordering Information

    Ordering Information Order No. Description Order No. Description 45-0101 Caliper Electrode, 1.0 x 1.0 cm Kit 1 Ea 45-0491 Tissue Slice Chamber, 7 x 7 mm Dish 1 Ea (needs 45-0492, 45-0503, 45-0204) 45-0102 Caliper Electrode, 2.0 x 2.0 cm, 1.5 x 1.5 cm Kit 1 Ea 45-0492 Tissue Slice Wand, (+) 7 mm...

  • Page 48: Warranty Information

    Without limiting the generality of the foregoing, BTX shall not be liable for any claims of any kind whatsoever, as to the equipment delivered or for non-delivery of equipment, and whether or not based on negligence.

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Gemini sc

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