bioMerieux DENSIMAT User Manual
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bioMerieux DENSIMAT User Manual

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Ref : 99535
Version C
Manuel d'utilisation DENSIMAT
DENSIMAT User's Manual
Gebrauchsanweisung DENSIMAT
Manuale d'Uso DENSIMAT
Manual de usuario DENSIMAT
Manual de utilização DENSIMAT
bioMérieux S.A. 69280 Marcy l'Etoile / France
Tel. 33 (0)4 78 87 20 00 - Fax 33 (0)4 78 87 20 90
http://www.biomerieux.com
Printed in France / 673 620 399 RCS Lyon
© 1995-2007.bioMérieux, S.A.

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Summary of Contents for bioMerieux DENSIMAT

  • Page 1 Manuel d'utilisation DENSIMAT DENSIMAT User's Manual Gebrauchsanweisung DENSIMAT Manuale d’Uso DENSIMAT Manual de usuario DENSIMAT Manual de utilização DENSIMAT bioMérieux S.A. 69280 Marcy l'Etoile / France Tel. 33 (0)4 78 87 20 00 - Fax 33 (0)4 78 87 20 90 http://www.biomerieux.com...
  • Page 3 English DENSIMAT User's Manual...
  • Page 5 R e v i s i o n s The list of revisions below summarizes replacements or additional pages in the DENSIMAT User's Manual. Version Date of printing Modifications Page(s) modified 10/95 Creation B rev. 09.03 09/03 CE marking 03/07 Insertion of an addendum in the section "Environment".
  • Page 7 33 (0)4 78 87 20 00 Seminario 131 Tokyo 107-0061 fax 33 (0)4 78 87 20 90 Providencia tel. (81) 3 5411 87 11 http://www.biomerieux.com Santiago fax (81) 3 5411 87 10 tel. (56) 2634 20 92 Germany Korea fax (56) 2634 20 93 bioMérieux Deutschland GmbH...
  • Page 8 Mexico Portugal Thaïland bioMérieux México SA de CV bioMérieux Portugal, Lda. bioMérieux Thaïland Ltd Chihuahua 88, col. Progreso Rua Alto do Montijo, Regent House Bldg, 16 th Floor México 01080, D.F. Lotes 1 e 2 - Portela de Carnaxide 183 Rajdamri Road, Lumpini, Pathumwan tel.
  • Page 9 4501-1576-C en This manual is periodically updated. The updates shall be included in the new releases of the Software. Information supplied in this manual may be subject to modifications before the products described become available. This manual contain information references relating certain bioMérieux S.A.
  • Page 10 bioMérieux, the blue logo API, and ATB are used, pending and/or registered trademarks belonging to bioMérieux SA or one of its subsidiaries. PANASONIC is a used, pending and/or registered trademark belonging to Matsushita Electric Industrial Co. Ltd. Corporation.
  • Page 11 IMPORTANT! The user is advised to read and understand all instructions in this manual to be able to derive the best performance from the DENSIMAT. The accuracy of results obtained with this instrument depends, in particular, on the maintenance operations described in this manual.
  • Page 12 WARNING! Changes to the equipment not expressly approved by bioMérieux S.A. could void the user’s authority to operate the equipment. The user will be required to repair damage at his own expense. bioMérieux S.A. is in no case liable for any damage that may arise from failure to comply with technical specifications in this manual, from the handling of biological fluids or any operation conducted on the equipment not in compliance with these mandatory standards.
  • Page 13 Location ..............................6-1 Electrical adjustment..........................6-2 Autonomy of batteries ..........................6-2 7 Procedure Adjusting the inoculum..........................7-1 Precautions for use ..........................7-2 8 Verification of the accuracy 9 Cleaning DENSIMAT Cleaning..............................9-2 10 Maintenance 10-1 11 Glossary 11-1 12 Index...
  • Page 15 Note : Screen captures and figures are given as examples only. 4. Principle Fig. 4-1: DENSIMAT reading principle....................4-2 Fig. 4-2: DENSIMAT display panel ......................4-3 9. Cleaning DENSIMAT Fig. 9-1: Preparing the cleaning device....................9-3 User's Manual DENSIMAT II-1...
  • Page 17 This manual includes chapters which cover the description, installation, preparation prior to use, use and maintenance of the DENSIMAT CAUTION! The user is advised to read and understand all the instructions in this manual to be able to derive the best performance from the DENSIMAT. User's Manual DENSIMAT...
  • Page 18 The glossary is located in chapter 11. It gives the definition of the main technical terms used in the manual. Index The index is located in chapter 12 at the back of the manual. It is used to locate a particular description or procedure. User's Manual DENSIMAT...
  • Page 19 T y p o g r a p h i c c o n v e n t i o n s Following are the terms and visual cues used in this manual to aid in your understanding of the procedures. • Type The bullet point is used to denote an action to be performed. User's Manual DENSIMAT...
  • Page 20 How to use this manual Graphic symbols G r a p h i c s y m b o l s 4501-1582 A en The standard symbols used for the bioMerieux systems and their meanings can be found below: Direct current Alternating current...
  • Page 21 REINFORCED INSULATION (equivalent to Class II of IEC 536) Rrisk of electric shock Caution, consult accompanying documents High temperature Potential pinch point Biological risks Consult Instructions for Use Batch code Catalogue number Temperature limitation Authorised Representative in the European Community Keep dry User's Manual DENSIMAT...
  • Page 22 How to use this manual Graphic symbols Manufacturer Date of manufacture In Vitro Diagnostic Medical Device Serial number Keep away from magnetic field Separate collection for waste electrical and electronic equipment User's Manual DENSIMAT...
  • Page 23 D e s c r i p t i o n a n d u s e DENSIMAT is designed to measure the optical density of a bacterial inoculum produced in an ampoule of liquid medium. It gives values in McFarland units, proportional to the average values of bacterial concentrations obtained with Gram-negative rods isolated from clinical specimens.
  • Page 25 Each unit is an Li/SOCI2 type Lithium battery 3.6V nominal, size AA with a maximum nominal capacity of 1.8 A/H. − The DENSIMAT can also be supplied with current by one of the following AC power supply adapters or equivalent ones:...
  • Page 26 : 7.5 VDC, 250mA with output connector ® mod. PANASONIC Ø = 3.0 x 1.1 mm. − Light source : LED 950 nm Optical characteristics − Central photosensor − Lateral photosensor − Logarithmic display in McFarland units : 0.5 McF to 7.5 McF. User's Manual DENSIMAT...
  • Page 27 P r i n c i p l e DENSIMAT enables precise determination of bacterial density by two measurements. An incident beam of light is passed through the ampoule and two subsequent measurements are obtained: − Scattered light S (two photosensors), −...
  • Page 28 Principle Peripheral photosensors Light source (photosensor) Ampoule Central photosensor DIVISION scattered light RD = transmitte light RESULT PRESENTATION Scale graduated in McFarland units Fig. 4-1: DENSIMAT reading principle User's Manual DENSIMAT...
  • Page 29 Principle "Bar Graph" The number of squares displayed is proportional to the value in McFarland Units McFarland scale Ø Ø.5 1 McF Ampoule Ø.4 Battery charge indicator light Numerical value Fig. 4-2: DENSIMAT display panel User's Manual DENSIMAT...
  • Page 31 If possible, keep packaging materials in case the instrument has to be moved in the future. CAUTION! Any damage directly or indirectly resulting from the transport of the instrument without adequate containers will not be covered by the warranty or maintenance contract. User's Manual DENSIMAT...
  • Page 33 K i t c o n t e n t s See the manual provided by the manufacturer. L o c a t i o n DENSIMAT is designed to be used on a laboratory bench top, but may be moved depending on the needs of the user. •...
  • Page 34 Electrical adjustment E l e c t r i c a l a d j u s t m e n t DENSIMAT is designed to be used with two Lithium batteries (nominal 3.6 V) described in the technical specifications.
  • Page 35 DENSIMAT to perform the reading. − DENSIMAT goes into standby mode after 30 seconds if there is no change in the O.D. of the suspension. This mode is indicated by “McF” on the display. A d j u s t i n g t h e i n o c u l u m Most standardized methodologies recommend the use of bacterial suspension with a density equal to a McFarland value.
  • Page 36 The measurement of the bacterial density is performed in the lower part of the ampoule (up to 1 cm above the bottom of the ampoule). Should DENSIMAT be contaminated, the ampoule well must be cleaned (see "Cleaning DENSIMAT " on page 9-1).
  • Page 37 CAUTION! Following the withdrawal of the small ampoules from our catalogue, verification of the accuracy of the DENSIMAT must only be performed using the large ampoules in the ATB DENSITOMETER CONTROL KIT (product no. 15 512), according to the instructions for use of the kit (see the package insert).
  • Page 39 − if an ampoule has been broken inside the reading block, − if the results obtained are too high. − when maintenance is carried out (see Chapter 10) IMPORTANT! A test must be done each time DENSIMAT is cleaned to ensure that it gives correct results. User's Manual DENSIMAT...
  • Page 40 • Swab the photosensors situated in the right section of the reading block. • Test the instrument by referring to “Testing DENSIMAT ”. If the results are incorrect, repeat the cleaning procedure. Note: The cleaning device is available at bioMérieux S.A. under the reference: 4550409A.
  • Page 41 Cleaning DENSIMAT Cleaning - Cut the desired length - Insert the cotton swab in the device - Clean the photosensors Fig. 9-1: Preparing the cleaning device User's Manual DENSIMAT...
  • Page 43 1 0 M a i n t e n a n c e The maintenance of the optical block must be carried out once a month in addition to the reasons reported in the section "Cleaning DENSIMAT " on page 9-1.
  • Page 45 Sensor enabling a quantity of light received to be transformed into electrical PHOTOSENSOR current. STRIPS Set of standardised, miniaturised tests on a plastic support. TURBIDIMETRY Measurement of the intensity of transmitted light (T) which is inversely proportional to the bacterial growth. User's Manual DENSIMAT 11-1...
  • Page 47 Density ........................2-1, 3-1, 4-1, 7-1, 7-2, 11-1 McFarland......................2-1, 3-2, 4-1, 4-3, 7-1, 7-2, 11-1 Photosensor ........................4-1, 4-2, 8-1, 9-2, 11-1 Reading ..........................4-2, 6-1, 7-1, 8-1, 9-1 Suspension............................. 2-1, 4-1, 7-1 Value ..........................2-1, 4-1, 4-3, 7-1, 7-2 User's Manual DENSIMAT 12-1...
  • Page 49 N o t e s ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... User's Manual DENSIMAT Notes-1...
  • Page 50 bioMérieux, the blue logo, API and ATB, are used, pending and/or registered trademarks belonging to bioMérieux SA or one of its subsidiaries.

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