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BioSampler
Introduction
Description
Nozzle section*
3 Nozzles (sonic
orifi ces)
BioSampler shown with 20-ml vessel (5-ml vessel available)
* Etched nozzle sections and collection vessels are supplied as a matched set. Use the etched identifi er
to match the two components during sampler reassembly for optimal fi t and performance.
Bioaerosol Collection Device
®
Cat. No. 225 Series
Figure 1. SKC BioSampler Components
Operating Instructions
863 Valley View Road, Eighty Four, PA 15330 USA
Tel: 724-941-9701 • www.skcinc.com
Inlet
Inlet section
Outlet to pump
Collection
vessel*
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Summary of Contents for SKC biosampler 225-9595
- Page 1 Collection vessel* Figure 1. SKC BioSampler Components BioSampler shown with 20-ml vessel (5-ml vessel available) * Etched nozzle sections and collection vessels are supplied as a matched set. Use the etched identifi er to match the two components during sampler reassembly for optimal fi t and performance.
- Page 2 Checking BioSampler/Kit Contents Use the table below to verify that you received all items associated with the Cat. No. ordered. If you are missing items, contact SKC at 800-752-8472 (U.S. only) or 724-941-9701. If You Ordered Cat. No. Your Package Should Contain...
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Page 3: Operation
® Tubing Inlet Air Sampler Cat. No. 228-9605 Rotameter SKC Inc. Eighty Four, PA Valve adjust BioSampler Sample Pump (Vac-U-Go shown) Figure 2. Calibration Train If using the rotameter supplied in Cat. No. 225-9594, use a length of 3/8-inch ID silicone tubing to connect the rotameter outlet to the BioSampler inlet. - Page 4 Prepare the BioSampler Collection Vessel Before sampling, sterilize the BioSampler, choose a collection liquid or coating, and fi ll or coat the collection vessel as follows. Sterilize the BioSampler Sterilize the inlet, nozzle section, collection vessel, and ground joint cap in an autoclave or oven heated within a range of 320 to 356 F (160 to 180 C) for 180 to 240 minutes before each use.
- Page 5 • Sterile solution for coating (can include, but is not limited to, petroleum jelly/hexane solution) • Aluminum foil or Parafi lm a. In a sterile environment, prepare coating solution. Note: SKC recommends using a petroleum jelly/hexane solution (approximately 0.1 gram petroleum jelly per milliliter of hexane).
- Page 6 Set Up/Sample • If sampling using a collection liquid, especially a viscous liquid such as ViaTrap or glycerol, SKC recommends using Liquid Trap Cat. No. 225-22 to protect the sample pump. • If using a collection liquid, see Proper Operation During Sampling.
- Page 7 Outlet InHg ASHCROFT ® Tubing Air Sampler Cat. No. 228-9605 SKC Inc. Eighty Four, PA BioSampler (coated) Valve Sample Pump (Vac-U-Go shown) Figure 4. Sampling Train with Coated Collection Vessel Sample for the sampling period appropriate for the collection liquid or coating used.
- Page 8 Proper Operation During Sampling If sampling using collection liquid, observe the sampler frequently during sampling (especially when using viscous collection liquid) for the following. When operating properly, the collection liquid swirls up the inner wall of the collection vessel, wett ing the inner wall where the nozzles output.
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Page 9: Maintenance
Prepare Samples for Transport/Shipment and Analysis In a sterile environment, disassemble the BioSampler. Transfer sample as follows: a. If collection liquid was used, accurately measure the final volume of collection liquid and aseptically transfer it into a sterilized container for analysis. -
Page 10: Accessories/Replacement Parts
References Macher, J.M., et al., “Sampling Airborne Microorganisms and Aeroallergens,” Air Sampling Instruments for Evaluation of Atmospheric Contaminants, 8th ed., ACGIH, 1995, pp. 589-617 Macher, J.M., “Evaluation of Bioaerosol Sampler Performance,” Applied Occupational Environmental Hygiene, 12, 1997, pp. 730-736 Butt ner, M.P., et al., “Sampling and Analysis of Airborne Microorganisms,” Manual of Environmental Microbiology, ASM Press, Washington, DC, 1997, pp. - Page 11 SKC Limited Warranty and Return Policy SKC products are subject to the SKC Limited Warranty and Return Policy, which provides SKC’s sole liability and the buyer’s exclusive remedy. To view the complete SKC Limited Warranty and Return Policy, go to htt p://www.skcinc.com/warranty.asp.
- Page 12 www.skcinc.com Form 37084 Rev 1707...
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