Related Manuals for Peqlab peqSTAR 2X
Summary of Contents for Peqlab peqSTAR 2X
- Page 1 Instruction Manual Thermal cycler peqSTAR 2X Double block thermal cycler peqSTAR 96X, 384X and in situ X thermal cycler Creating the future together. v1212E...
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Page 3: Table Of Contents
Instruction Manual peqSTAR X thermal cyclers CONTENT page System Overview Safety instructions License Notice Installation Content of delivery Installing the thermal cycler Operation of the thermal cycler Connections of the thermal cyclers General Operation Main menu Programs Diagnostics GLPs System Help Button Run dialogue Run dialogue: Overview... - Page 4 Instruction Manual peqSTAR X thermal cyclers 9.1.3 New program 9.1.4 New folder 9.1.5 Delete 9.1.6 'Info' button Operating elements of the 'Programs' dialogue 9.2.1 Entering a command 9.2.2 List view 9.2.3 Diagram view 9.2.4 New program 9.2.5 Open a program 9.2.6 Info-Button 9.2.7...
- Page 5 Instruction Manual peqSTAR X thermal cyclers 12.6.1 Overtemp test 12.7 Firmware 12.7.1 Control Unit 12.7.2 Power Unit 12.8 Print 12.9 Display 12.9.1 Brightness 12.10 Protocol 12.11 LAN Control 12.11.1 New Lan Connect 12.11.2 Lan Connect 12.11.3 Lan Disconnect 12.11.4 Zigbee Configure 12.11.5 Zigbee Connect 12.11.6 Zigbee Disconnect 12.12 USB Recovery...
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Page 6: System Overview
Instruction Manual peqSTAR X thermal cyclers SYSTEM OVERVIEW The peqSTAR X thermal cyclers are the ideal PCR devices for all molecular biological and biochemical laboratories working in the field of basic research or routine diagnostic. The 250 °C HTR (High Temperature Range) Peltier technology allows quick changes in temperature up to 5 °C/sec (max). -
Page 7: License Notice
Instruction Manual peqSTAR X thermal cyclers LICENSE NOTICE This instrument is licensed for research and development and for uses other than human in vitro diagnostics under one or more of the following patens of Applera Corporation: U.S. Patent Nos. 5,656,493, 5,038,852, 5,333,675, 5,475,610 (claims 1-159 and 164-166), 6,703,236 (claims 7-10) and 7,238,517 or corresponding claims in their non-U.S. -
Page 8: Connections Of The Thermal Cyclers
Instruction Manual peqSTAR X thermal cyclers Connections of the thermal cyclers 2x USB interface 2x USB interface Mains switch Mains power inlet Ethernet interface GENERAL OPERATION The operation of the thermal cycler can be run by the internal touch screen, either using the touch sensitive surface of the display or by using a mouse connected to a free USB port. -
Page 9: Main Menu
Instruction Manual peqSTAR X thermal cyclers MAIN MENU The main menu consists of five dialogues, which can be selected via tabs at the right side of the screen. PeqSTAR 2X thermal cyclers offer two tabs 'L' (left) and 'R' (right) for choosing the desired block. This option is not available for peqSTAR 96X, 384X und in situ X systems. -
Page 10: Run Dialogue
The Run dialogue is used for running and monitoring existing PCR protocols. Run dialogue: Overview The following functions are found in the 'Run' dialogue: Block selection left / right Program window (only for peqSTAR 2X systems) Direct help List view / diagram view Program name... -
Page 11: Info Button
Instruction Manual peqSTAR X thermal cyclers 7.2.5 Info button Use this button to display details of a PCR protocol (name, program options, author, settings, comments and program steps). 7.2.6 Lid lock / unlock Use this button to lock or unlock the lid. Furthermore the button shows the current status of the lid. -
Page 12: Program Options
Instruction Manual peqSTAR X thermal cyclers 7.4.2 Program Options Using this tab, the settings of the selected program are shown. 7.4.3 Print With peqSTAR X thermal cyclers, PCR programs and GLPs could be printed on a network printer. Using this dialogue, you can type in the path for a printer in the network. -
Page 13: A Program Is Running
Instruction Manual peqSTAR X thermal cyclers A program is running 7.7.1 General: 'Pause' and 'Stop' button After starting the chosen program, you can use these buttons to either pause or to stop the program. 7.7.2 'Pause' button If the 'Pause' button is pressed during the operating sequence, the adjoining window appears. -
Page 14: Program Window
Instruction Manual peqSTAR X thermal cyclers 7.7.5 Program window In the program window the blue flashing bar is showing the actual program step. 7.7.6 Save GLP Report If stopping the program after the security query, the adjoining query to create a GLP report appears. Note: The GLP report query also appears when a program is finished. -
Page 15: Incubation Mode
Instruction Manual peqSTAR X thermal cyclers INCUBATION MODE Run incubation Use this button to set the device into the incubation mode. The temperature for the thermal plate and lid can be set manually. Setting the block and lid temperature Set temperatures of block and lid by entering the values of choice in the corresponding editor fields. -
Page 16: Programs
Instruction Manual peqSTAR X thermal cyclers PROGRAMS The 'Programs' dialogue serves for generating and editing PCR protocols (programs). This is a list of commands, which the thermal cycler performs in sequence after starting the program. The 'Programs' dialogue provides an editor for this purpose. -
Page 17: New Program
Instruction Manual peqSTAR X thermal cyclers By using the 'Copy' button the following dialogue appears: List of the selected files to copy Destination for the copied files (desired folder has to be selected by clicking) In this dialogue the directory can be selected. Copy the files with 'OK' or quit the operation with 'Cancel'. -
Page 18: Operating Elements Of The 'Programs' Dialogue
Instruction Manual peqSTAR X thermal cyclers Operating elements of the 'Programs' dialogue Direct help Name of the PCR pro- List of available Program window gram and total time commands The 'Program window' 'Program window' displays the list of commands (steps) the program is composed of. The 'List of available 'List of available 'Program window' 'Program window'... -
Page 19: New Program
Instruction Manual peqSTAR X thermal cyclers 9.2.4 New program A new program can be generated, after pushing this button the program’s name has to be defined. Optionally author, user, any comment and the program options can be entered. 9.2.4.1 New Cyclerprogram Enter the name of the PCR program, optionally also add name of the author and a comment. - Page 20 Instruction Manual peqSTAR X thermal cyclers 9.2.4.5 Gradient Control Using the Checkbox you can decide whether in a gradient step the plateaus (Plateaus synchronized) or the Ramps (Ramps synchronized) should be synchronized for all rows. 9.2.4.6 Tube Control With the Checkbox you have the possibility to adjust temperature regulation for a temperature step accordingly to sample volume.
- Page 21 Instruction Manual peqSTAR X thermal cyclers How to emulate a protocol ABC from cycler XY on a peqSTAR in the right way? Enter the protocol ABC in the program editor Program: of the peqSTAR the same way you did it on the cycler XY.
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Page 22: Open A Program
'Yes'. The program is then loaded to the 'Run' tab and started. At the peqSTAR 2X the desired block can be chosen by using this dialogue. 9.2.10 Editing a command The parameters of a command can be modified by touching the corresponding command line of the program and changing it with the 'Edit' button. -
Page 23: Available Commands (Program Steps)
Instruction Manual peqSTAR X thermal cyclers Available commands (program steps) 9.3.1 Unlock Use this command to unlock the lid in case that it has been locked in a previous program step. Unlock 9.3.2 Lock Use this button for lid locking in order to Lock avoid accidental opening while a program is running. -
Page 24: Temperature
Instruction Manual peqSTAR X thermal cyclers 9.3.4 Temperature Use this button to insert a temperature step in the PCR program. Temp 9.3.4.1 Temperature Set the temperature, which should be held during the step. 9.3.4.2 Time Set the time how long the desired temperature should be held. - Page 25 Change of time within a program cycle. The sign here defines whether the time will elongate or reduce. For the peqSTAR 2X the gradient is realised over the 8 rows of the block from the top to the bottom (as displayed in 9.3.6.1).
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Page 26: Cycle
Instruction Manual peqSTAR X thermal cyclers 9.3.7 Cycle: Use this button to define a program cycle Cycle (loop), consisting of several individual program steps. In the field 'Number of Cycles' the amount of cycles is entered. In the following dialogue the commands 'Temp' and 'Gradient' can be entered in the cycle. -
Page 27: Diagnostics
Different operating elements for indication and navigation within a diagram are available: Display of the selected zones (8 zones each block for Selection of single view or the peqSTAR 2X, 16 zones for the peqSTAR 96X) complete view of the eight zones Help Mode... -
Page 28: General View
Instruction Manual peqSTAR X thermal cyclers 10.2 General view General view of the zones of the thermal plate showing the temperature profile of the eight or 16 zones in one combined diagram (see 10.1) when choosing the 'Diagnostics' dialogue. The temperature profile of the single zones is shown in one window lying upon another. -
Page 29: Glps Dialogue
Instruction Manual peqSTAR X thermal cyclers 11 GLPS DIALOGUE The 'GLPs' dialogue is used for the administration, viewing and printing of the GLP reports generated by the thermal cycler. 11.1 Displaying a GLP report After pushing the GLP button a dialogue appears to select a GLP report, which will be directly shown. The dialogue can also be opened by pushing the button Shows the name of the selected GLP report. -
Page 30: Delete
Instruction Manual peqSTAR X thermal cyclers 11.2.4 Delete Use this button to delete a GLP report. 11.2.5 Copy Pushing this button saved GLP reports or files can be copied. Several GLP reports/files can be marked via mouse click simultaneously if the option 'Multiple Selection' was chosen. -
Page 31: System
Instruction Manual peqSTAR X thermal cyclers 12 SYSTEM In this dialogue the configuration and settings of general parameters of the device can be carried out. Elements of the 'System' dialogue: Overview Having selected one of the symbols the function is called up and the corresponding settings can be executed or changed as explained in the following dialogue. -
Page 32: Email
Instruction Manual peqSTAR X thermal cyclers 12.3.1.1 Repetitive When this checkbox is activated, the user call will be repeated periodically as long as the event is open. 12.3.1.2 Sound intensity: Set the desired sound intensity. 12.3.1.3 Select sounds By pushing the button a selection window will appear. -
Page 33: User Management
Instruction Manual peqSTAR X thermal cyclers 12.3.2.3 User name Please enter the user name of the used email account. Please note that you have to enter the SMTP settings here. In case you have defined different user names for incoming and outgoing mail server in your account settings, enter here the user name for the outgoing mail server. -
Page 34: Change User
Instruction Manual peqSTAR X thermal cyclers After pressing the button the adjoining dialogue appears: To call up the user administration the user has to log in with his password. The user (in general the administrator) must have the appropriate right for the administration of users. -
Page 35: Setting Up A New User Group
Instruction Manual peqSTAR X thermal cyclers 12.4.2.2 Set up a new user and assign a password Use this button to set up a new user. The adjoining dialogue window appears where several data can be added. Save the data with the button and the newly set up user is displayed in the user list among the related user group. -
Page 36: User Logout
Instruction Manual peqSTAR X thermal cyclers List of available rights: List of available rights: List of available rights: List of available rights: Run incubation Run incubation mode Edit programs Edit programs Start programs Start programs GLP management Watch GLP reports User management Administrate user and user groups System administration... -
Page 37: Network Settings
Instruction Manual peqSTAR X thermal cyclers 12.5 Network Settings Using this button, network settings can be changed. At the tab the adjoining dialogue appears and the required settings for network integration can be entered. Please ask your network administrator for the required settings. -
Page 38: Hardware
‘Yes’. Initialization and overtemperature test is done automatically for both blocks at the peqSTAR 2X. Press OK after the test has finished. If one or both results are not “OK”, the overtemperature protection is damaged. In In In In... - Page 39 Instruction Manual peqSTAR X thermal cyclers 12.7.1.2 Settings Pushing this button the adjoining dialogue appears. The following menu items are available: 'Programs…', 'GLP…' and 'Factory Settings'. 12.7.1.2.1 Programs Settings Setting a check mark in this box, a 'Lid Heat' step will be automatically inserted as first step when generating a new program.
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Page 40: Power Unit
Instruction Manual peqSTAR X thermal cyclers Important: Important: Important: Important: The USB memory stick may not be removed during update proces The USB memory stick may not be removed during update proces The USB memory stick may not be removed during update proces The USB memory stick may not be removed during update process! s! s! s! After a successful update the display shows the corresponding message and the software starts again. -
Page 41: Display
Instruction Manual peqSTAR X thermal cyclers 12.9 Display Pushing this button, the adjoining window opens: 12.9.1 Brightness The following dialogue appears after pressing the button: By pressing the buttons or moving the roll bar the backlight of the display can be adjusted. 12.10 Protocol For troubleshooting, the thermal cycler can record its own internal communication. -
Page 42: Lan Connect
As soon as a cycler was once remote controlled via LAN connection, it will be displayed in the LAN control window (in our example: peqSTAR 2X 192.168.0.121) and marked as non connected. For a new LAN connection with this cycler, mark it in the overview and use the button 'LAN Connect' to start a new connection. -
Page 43: Zigbee Connect
Instruction Manual peqSTAR X thermal cyclers COM Interface COM Interface PC name, PC name, Network level Network level COM Interface COM Interface PC name, PC name, Network level Network level As COM number use the that is shown at the Default setting is '1' for COM port where the ZigBee thermal cycler... -
Page 44: Zigbee Disconnect
Instruction Manual peqSTAR X thermal cyclers If this message is confirmed with 'Yes' and therefore the remote control authorized, the following message appears on the control unit. 'User from IP Address Remote Cycler has accepted LAN remote.' If a check mark is set at 'Always accept remote control' under 'System' => 'Network Settings' =>... -
Page 45: Maintenance & Repair Of The Thermal Cycler
Instruction Manual peqSTAR X thermal cyclers 13 MAINTENANCE & REPAIR OF THE THERMAL CYCLER 13.1 Cleaning, maintenance and repair of the thermal cycler For safety reasons the device must be switched off and the mains plug pulled before cleaning is carried out! For safety reasons the device must be switched off and the mains plug pulled before cleaning is carried out! For safety reasons the device must be switched off and the mains plug pulled before cleaning is carried out! For safety reasons the device must be switched off and the mains plug pulled before cleaning is carried out! -
Page 46: Disposal Instruction
This product is subject to the WEEE disposal regulations and cannot be disposed of with regular waste. Please contact PEQLAB Biotechnologie GmbH or your local distributor for disposal of peqSTAR X thermal cyclers. Old electronic equipment is not useless waste. Environmentally friendly disposal can help to retrieve valuable resources. -
Page 47: Block Capacity
Master-/Slave-control (optionally also wireless available) 16.4 Block capacity peqSTAR 2X/2X Gradient: Two 48 well Universal blocks with: lid heating and automatic height adaption for 48 x 0.2 ml tubes, 48 well PCR plates or 24 x 0.5 ml tubes with flat caps. - Page 48 PEQLAB Biotechnologie GmbH, 91052 Erlangen, Freecall (D): 0800 100 20 16, info@peqlab.de, www.peqlab.de PEQLAB Biotechnologie GmbH, 6404 Polling, Tel: +43 (0) 5238 84 169, info@peqlab.at, www.peqlab.at PEQLAB Ltd., Southampton SO31 7ZN, Freephone (UK): 0808 202 1302, info@peqlab.co.uk, www.peqlab.co.uk USA PEQLAB LLC, Wilmington, DE 19810, Toll-Free (US): 877 737 5220, info@peqlab.us, www.peqlab.us Creating the future together.