Table of Contents
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Table of Contents
Troubleshooting
Related Manuals for Buchi Alltech 3300 ELSD
Summary of Contents for Buchi Alltech 3300 ELSD
- Page 1 Alltech Model 3300 ELSD ® Operation Manual 11593900A en...
- Page 2 CH-9230 Flawil 1 E-Mail: quality@buchi.com BUCHI reserves the right to make changes to the manual as deemed necessary in the light of experi- ence, especially with respect to structure, illustrations and technical detail. This manual is copyrighted. Information from it may not be reproduced, distributed, or used for competi- tive purposes, nor made available to third parties.
- Page 3 The Warning symbol indicates a hazardous situation that, if not avoided, could result in death or serious injury. The Caution symbol indicates a hazardous situation that, if not avoided, could result in minor or moderate injury. The Notice symbol is used to highlight information that will optimize the use and reliability of the system. www.buchi.com...
- Page 4 • If the unit is damaged and does not function properly, stop the unit safely and contact the manufacturer immediately. • Class 3B LASER radiation inside can cause severe eye damage. Do not open or defeat interlocks. Avoid exposure to the beam. www.buchi.com...
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Page 5: Table Of Contents
3.4.3 Display ................................12 3.4.4 Date and Time ..............................12 3.4.5 Language ................................12 3.4.6 Pressure Units ..............................12 3.4.7 Network ................................13 3.4.8 Timed Mode Changes ............................13 3.5 Maintenance ............................13 3.5.1 Manual Control..............................13 3.5.2 Logs .................................. 14 www.buchi.com... - Page 6 6.3 Diagnosing Baseline Noise ......................... 25 6.4 Troubleshooting Charts ........................26 7. Appendix ..........................28 7.1 Specifications ............................28 7.2 Replacement Parts ..........................28 7.3 Volatile Mobile Phase Modifiers ......................29 8. Contact BUCHI ........................30 9. Useful References ......................31 www.buchi.com...
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Page 7: Introduction
Method Wizard for simplified method development, multiple languages, and built- in Diagnostic tests for troubleshooting. The Model 3300 ELSD also features compact, stackable dimensions, allowing it to fit easily into limited laboratory space. www.buchi.com... -
Page 8: Principle Of Operation
Larger droplets scatter more light and increase the sensitivity of the analysis, but they are also more difficult to evaporate in the drift tube. There will be an optimum gas flow rate for each method which will produce the highest signal-to-noise ratio. www.buchi.com... -
Page 9: Installation
Any damage to the container or its contents should be reported immediately to your local BUCHI Customer Service office which can be • A guard column or cartridge compatible with the found on the BUCHI website at: www.buchi.com. Please separation column is recommended to prolong refer to Section 7.5, Warranty, Returns, and Repairs, for... -
Page 10: Controls And Features
Refer to towards you. Section 5.3 for details. 6. Nebulizer: The column effluent and nitrogen gas are combined within the nebulizer to form an aerosol. The nebulizer is easily removed for cleaning by using a counterclockwise twisting motion. www.buchi.com... -
Page 11: Back Panel (Figure 2.2)
10. Gas Inlet: The GAS INLET accepts the nebulizer gas service tech functions. supply tubing. The inlet features a quick-connect port, which requires no additional fittings. Gas pressure should 6. 14-Pin Connector: Outputs TTL/contact closure signals be regulated from 65 – 80psig. or accepts signals from peripheral equipment. www.buchi.com... -
Page 12: Making Electrical And Fluid Connections
Consult the appropriate manuals for wiring There should be no low spots in the tubing where information. condensate can collect. Make sure the exhaust system provides adequate but not excessive suction. Excessive suction can cause a noisy baseline. www.buchi.com... -
Page 13: Navigating The Software Interface
Select Operation/Mode/Run and press Enter to put the detector into Run mode. In Run mode, the heaters, gas flow, and laser are on. The signal output is displayed and the chromatogram trace is active. Run mode is used for sample analysis. www.buchi.com... -
Page 14: Start/Stop Run
Accept to save the new chart view, or press Cancel to return to the original chart view. The x-axis range is –60 to 0 minutes. The y-axis range is –500mV to 2.5V. To reach Pan directly from Zoom, press Enter. www.buchi.com... -
Page 15: Autozero
The Reset Scale function will zoom out the chart view to its maximum x-axis and y-axis values: -60 to 0 min; -500mV to 2.5V. Select Operation/Chart/Reset Scale and press Enter to reset the chart axes. www.buchi.com... -
Page 16: Open
Cancel to cancel deleting the method. Use the up/down arrows or the keypad to select a method number for the new method. Press Accept to save the new method, or press Cancel to cancel the new method. www.buchi.com... -
Page 17: Wizard
Use the left/right arrows to select the sample concentration Select Configuration/Outputs and press Enter to configure the from the list. Press Continue to reach the next Method outputs on the unit. Wizard screen, or press Cancel to cancel using the Method Wizard. www.buchi.com... -
Page 18: Display
Korean, Chinese (Simplified), and Japanese. The unit must have the correct software versions installed in order to be Custom Autozero compatible with certain languages; contact BUCHI for details. Use the left/right arrows to select Enabled or Disabled for the Select Configuration/Language and press Enter to bring up Custom Autozero. -
Page 19: Network
Manual Control screen. EDIT TIMED MODE CHANGE Laser: On/Off Press 1 to toggle the Laser On or Off. The current signal output value is also displayed in mV. Edit Timed Mode Change Event, select Configuration/Timed Mode Changes/Edit and press Enter. www.buchi.com... -
Page 20: Logs
Files can be imported or exported from the unit using a pen drive inserted into the USB Host port on the back panel of the unit. NOTE: The pen drive must be 128MB or less; pen drives with higher memory are NOT compatible with the 3300 ELSD. www.buchi.com... -
Page 21: Routine Operation
9. If the noise is higher than expected, you may want to perform the optics test to determine if there is a possible laser or electronics problem. Refer to Section 6.2.3 for details on the optics test. www.buchi.com... -
Page 22: Shutdown Sequence
Flush the column before reconnection. Instrument power may be left on when the instrument is not in use. The laser may be turned by putting the detector into Standby or Heating mode. www.buchi.com... -
Page 23: Maintenance
12. Replace the nebulizer wear band and nebulizer gas inlet. 13. Replace the nebulizer back into the unit by aligning the grooves and turning clockwise until the nebulizer locks firmly into place. 14. Replace the liquid and gas inlet lines to the nebulizer. 15. Replace the front door. www.buchi.com... -
Page 24: Drift Tube Cleaning Procedure
The inside of the flooding could occur inside the unit. optics block should be black. 8. Replace the nebulizer and reattach the liquid and gas tubing. Refer to Section 5.2, Steps 13 – 15. www.buchi.com... -
Page 25: Fuse Replacement
BUCHI Customer Service office which 1. Power off the detector from the back panel and unplug the can be found on the BUCHI website at: www.buchi.com. power cord. 2. Gently squeeze the side tabs of the fuse drawer and pull Pre-Amp the fuse drawer straight out. -
Page 26: Diagnostics And Troubleshooting
Heater, thermal fuse, or ° Customer Service office which error. reading is more than 10 electronics error. can be found on www.buchi.com. (+/-) off its set point. Contact your local BUCHI Optics block temperature Optics block temperature Heater, thermal fuse, or °... -
Page 27: Performing Diagnostic Tests
Refer to too high. Section 5.2 for details. Contact your local Nebulizer gas BUCHI Customer pressure sensor Service office which error and/or other can be found on electronics error. www.buchi.com. 4. Press Test and the Nebulizer Gas Pressure Test will begin. -
Page 28: Gas Flow Test
3. In the software, select Maintenance/Tests/Gas Flow Test necessary. and press Enter. The testing screen will then appear: Contact your local BUCHI Customer Gas flow meter Service office which and/or valve can be found on error. -
Page 29: Optics Heating Test
4. The test takes 60 seconds to complete and a timer bar OPTICS TEST RESULTS appears indicating how much time is remaining on the test. RESULT CAUSE SOLUTION Contact your local BUCHI Fail: Noise level Electronics error. Customer Service office at laser off is too which can be found on high. - Page 30 Detector is still Wait for the detector equilibrating. to equilibrate and then repeat the test. Contact your local BUCHI Thermal fuse may Customer Service office be blown. which can be found on www.buchi.com. Optics block...
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Page 31: Diagnosing Baseline Noise
Result: Noise disappears when laser is turned off. 1. Possible electrical problem. Contact your local E. Noise from Electronics BUCHI Customer Service office which can be found • Column removed on www.buchi.com. • Mobile phase off •... -
Page 32: Troubleshooting Charts
Nebulizer may be partially obstructed. Refer to Section 5.2 for nebulizer cleaning procedure. Drift tube temperature not Thermal fuse(s) may be blown. Contact your local BUCHI Customer Service office reaching set point Drift tube heater, sensor, or other which can be found on www.buchi.com. - Page 33 BÜCHI Labortechnik AG TROUBLESHOOTING PROBLEM CAUSE SOLUTION Contact your local BUCHI Customer Service office No LCD display Electrical problem. which can be found on www.buchi.com. No peak(s) detected Sample is below the detection limit. Increase the sample concentration or injection volume and re-inject.
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Page 34: Appendix
Power Requirements: 120/240V, 50/60Hz, 5A Dimensions: 10.0" H x 10.25" W x 19.0" D (25.4cm H x 26.0cm W x 48.3cm D) Weight: 30 lbs (13.6 kg) Environmental Operating Temperature: 15 to 40˚C Relative Humidity: 10 – 90%, Non-Condensing www.buchi.com... -
Page 35: Volatile Mobile Phase Modifiers
111°C Pyridinium Acetate 4.0-6.0 Ammonium Carbonate 8.0 (adjusted) (used for reverse phase) Ammonium Carbonate 5.5-7.5 and 9.3-11.3 Ion-Pair Reagents Pentafluoropropionic Acid ~0.6 96-97°C Heptafluorobutyric Acid ~0.6 120°C Nonafluoropentanoic Acid ~0.6 140°C Pentadecafluorooctanoic Acid ~0.6 189°C Tridecafluoroheptanoic Acid ~0.6 175°C www.buchi.com... -
Page 36: Contact Buchi
BÜCHI Labortechnik AG 8. CONTACT BUCHI Your local BUCHI Customer Service office which can be found on www.buchi.com. Email: buchi@buchi.com www.buchi.com... -
Page 37: Useful References
Macrae and J. Dick, Journal of Chromatography, 210, 138-145 (1981). High-Performance Liquid Chromatographic Deter-mination of Mono- and Oligosaccharides in Vegetables with Evaporative Light-Scattering Detection and Refractive Index Detection, J. Lopez Hernandez, M.J. Gonzalez-Castro, I. Naya Alba, and C. de www.buchi.com... - Page 38 Kyoichi Osada, Amir Ravandi, and Arns Kuksis, Journal of the American Oil Chemists' Society, 76(7), 863-871 (1999). Reversed-Phase Separations of Nitrogenous Phospholipids on an Octadecanoyl Poly (Vinyl Alcohol) Phase, S.L. Abidi and T.L. Mounts, Journal of Chromatography A, 773, 93-101 (1997). www.buchi.com...
- Page 39 Column with Evaporative Light Scattering Detection, M.D. Lantz, D.S. Risley, and J.A. Peterson, Journal of Liquid Chromatography & Related Technologies, 20(9), 1409-1422 (1997). High-Throughput Characterization of Combinatorial Libraries Generated by Parallel Synthesis, J.N. Kyranos and J.C. Hogan, Analytical Chemistry, 70(1), 389A-395A (1998). www.buchi.com...
- Page 40 Wood Surfaces by HPLC with Evaporative Light Scattering Detection, C.R. Daniels, Journal of Chromatographic Science, 30, 497-499 (December 1992). Newer Method for the Characterization of Higher Molecular Mass Coal Derivatives, Keith D. Bartle, Erdol und Kohle - Erdgas - Petrocheme Vereinigt mit Brennstoff, 36(1), (January 1983). www.buchi.com...
- Page 42 T +55 19 3849 1201 F +66 2 862 08 54 F +971 4 313 2861 F +55 19 3849 2907 bacc@buchi.com middleeast@buchi.com latinoamerica@buchi.com www.buchi.com www.buchi.com www.buchi.com We are represented by more than 100 distribution partners worldwide. Find your local representative at: www.buchi.com...
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