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ECO Fluorometer
ECO FL
User's Guide
The user's guide is an evolving document. If you find sections that are unclear, or missing
information, please let us know. Please check our website periodically for updates.
WET Labs, Inc.
P.O. Box 518
Philomath, OR 97370
541-929-5650
fax: 541-929-5277
www.wetlabs.com
ECO FL User's Guide (FL)
Revision AN
4 June 2012
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Summary of Contents for Wet Labs ECO FL
- Page 1 The user’s guide is an evolving document. If you find sections that are unclear, or missing information, please let us know. Please check our website periodically for updates. WET Labs, Inc. P.O. Box 518 Philomath, OR 97370 541-929-5650 fax: 541-929-5277 www.wetlabs.com ECO FL User’s Guide (FL) Revision AN 4 June 2012...
- Page 3 To return the instrument, contact WET Labs for a Return Merchandise Authorization (RMA) and ship in the original container. WET Labs is not responsible for damage to instruments during the return shipment to the factory. WET Labs will supply all replacement parts and labor and pay for return via 3 day air shipping in honoring this warranty.
- Page 4 ECO FL User’s Guide (FL) Revision AN 4 June 2012...
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Page 5: Table Of Contents
Column Description ......................19 Sample Device File ......................20 Sample Output Files ......................21 Appendix A: Mounting Bracket Drawing ................22 WET Labs WEEE Policy ......................23 WEEE Return Process ......................... 23 ECO FL User’s Guide (FL) Revision AN 4 June 2012... -
Page 7: Overview
Overview WET Labs offers a range of the Environmental Characterization Optics (ECO) series fluorometers for the following measurements: chlorophyll, Colored Dissolved Organic Matter, uranine, rhodamine, and phycocyanin and phycoerythrin. This section includes meter and connector specifications, delivered items and descriptions of features, including internal batteries and Bio-wiper™. -
Page 8: Connectors
Analog out WARNING If you are going to build or use a non-WET Labs-built cable, do not use the wire from pin 3 or the ECO meter will be damaged. Input power of 7–15 VDC is applied to pin 4. The power supply current returns through the common ground pin. -
Page 9: Delivered Items
The rate of closure and opening is dependent upon both temperature and depth. ECO FL User’s Guide (FL) Revision AN 4 June 2012... - Page 10 ECO meters are optionally equipped with a strain gauge pressure sensor. The pressure sensor is calibrated at WET Labs and the calibration coefficients are supplied on the instrument’s calibration sheets. Pressure sensor output is in counts and can be converted into engineering units using the instruments device file and ECOView software or the raw data can be converted in the user's software (e.g.
- Page 11 5. Screw the Swagelock fitting into the end flange until finger tight. 6. Tighten it an additional 1/8 turn using a wrench only if necessary. 7. Wipe up any excess oil. ECO FL User’s Guide (FL) Revision AN 4 June 2012...
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Page 12: Theory Of Operation
Fluoresced light is received by a detector positioned where the acceptance angle forms a 140- degree intersection with the source beam. An interference filter is used to discriminate against the scattered excitation light. Optical configuration of ECO fluorometer ECO FL User’s Guide (FL) Revision AN 4 June 2012... -
Page 13: Instrument Operation
Connect the 6-socket connector on the optional test cable to the instrument to provide power to the LEDs and electronics (see Section 1 for a diagram of the pin-outs of ECO FL). Connect the battery leads on the test cable to the 9V battery supplied with the meter. Light should emanate from the meter. -
Page 14: Bio-Wiper™ Operation
5. If the meter does not light after performing step 3, check the battery. Replace if necessary, perform steps 2 and 3 to verify communication. If it still does not light, contact WET Labs. Refer to the ECOView User’s Guide for details about using the software. -
Page 15: Deployment
Upkeep and Maintenance We highly recommend that ECO meters be returned to the factory annually for cleaning, calibration and standard maintenance. Contact WET Labs or visit our website for details on returning meters and shipping. After each cast or exposure of the instrument to natural water, flush with clean fresh water, paying careful attention to the sensor face. - Page 16 9. Slide the Bio-wiper™ over the shaft. Be careful not to twist it on, thus rotating the shaft. If the wiper does not slide on easily, insert the screw into the expander hole, turning slowly until the Bio-wiper™ slides easily onto the shaft. ECO FL User’s Guide (FL) Revision AN 4 June 2012...
- Page 17 180 degrees to cover the optics after sampling. 14. If the wiper needs adjusting, loosen the screw, make any necessary adjustments, and repeat steps 9 through 13 to ensure the wiper is performing properly. ECO FL User’s Guide (FL) Revision AN 4 June 2012...
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Page 18: Flb And Flsb: Using Internal Batteries
WET Labs Inc. disclaims all product liability from the use or servicing of this equipment. WET Labs Inc. has no way of controlling the use of this equipment or of choosing qualified personnel to operate it, and therefore cannot take steps to comply with laws pertaining to product liability, including laws that impose a duty to warn the user of any dangers involved with the operation and maintenance of this equipment. -
Page 19: Replacing End Flange And Batteries
8. Push the end flange all the way on to the pressure housing, making sure no wires are pinched. Be sure the vent plug does not pop up. If it does, you’ll need to re-position the foam spacer. 9. Re-insert the monofilament. ECO FL User’s Guide (FL) Revision AN 4 June 2012... -
Page 20: Checking Vent Plug
Rotate the vent plug to begin tightening the screw. Finish tightening using a screwdriver, being careful not to overtighten truss screw. Note A portion of the truss screw head has been removed to allow for venting in case of pressure buildup. ECO FL User’s Guide (FL) Revision AN 4 June 2012... -
Page 21: Data Analysis
The scale factor is then applied to the output signal to provide the direct conversion of the output to chlorophyll concentration. WET Labs supplies a scale factor that can be found on the instrument-specific calibration sheet that ships with each meter. While this constant can be used to obtain approximate values, field calibration is highly recommended. -
Page 22: Digital Response
= raw counts output when measuring a sample of interest output = dark counts, the measured signal output of meter in clean water with black tape over the detector Scale factor = multiplier in g/l/counts or ppb/counts ECO FL User’s Guide (FL) Revision AN 4 June 2012... -
Page 23: Characterization And Testing
(7–15V), a voltage test is performed at 7 and 15V, and the current draw and operation is observed. The current must remain constant at both 7 and 15V. ECO FL User’s Guide (FL) Revision AN 4 June 2012... -
Page 24: Terminal Communications
0 to 65535 Number of packets in a set $sto none Stores current settings to internal flash $ugl 0 to 255 µg/l conversion value (calculates slope x 10,000). Fluorometer only. ECO FL User’s Guide (FL) Revision AN 4 June 2012... -
Page 25: Device And Output Files
Valid Column Descriptions are listed in the subsections below. 8.3.1 Fluorescence Measurements CHL=x IENGR=x PHYCOERYTHRIN=x URANINE=x sc RHODAMINE=x CDOM=x 8.3.2 Miscellaneous Date=x MM/DD/YY Time=x HH:MM:SS N/U=x The column is Not Used ECO FL User’s Guide (FL) Revision AN 4 June 2012... -
Page 26: Sample Device File
Sample Device File Below is a sample device file without engineering output for an ECO FL with an external thermistor. FLSB-322 Created on: 6/23/2008 chl=ug/l "iengrunits = µg/l for CHL. ppb for CDOM, uranine, PE."... -
Page 27: Sample Output Files
Below is a sample device file with engineering output for an ECO FL with an external thermistor. FLSB-322 Created on: 06/23/08 iengrunits=µg/l iengrscaleoffset=5 chl=ug/l "iengrunits = µg/l for CHL, PC, PE. Ppb for CDOM and uranine." column 5 = input scale factor and offset. -
Page 28: Appendix A: Mounting Bracket Drawing
(MM/DD/YY) (HH:MM:SS) (µg/l) (counts) 6/14/2008 7:57:55 26.99 5194 3535 6/14/2008 7:57:56 26.91 5199 3525 6/14/2008 7:57:57 27.06 5201 3544 6/14/2008 7:57:58 26.82 5202 3513 Appendix A: Mounting Bracket Drawing ECO FL User’s Guide (FL) Revision AN 4 June 2012... -
Page 29: Wet Labs Weee Policy
WET Labs WEEE Policy In accordance with Directive 2002/96/EC and the Council of 27 January 2003, WET Labs policy regarding the collection and management of Waste Electrical and Electronic Equipment (WEEE) is published here and is available at www.wetlabs.com. A core component of our corporate vision is to accept responsibility for preserving our environment and we embrace the opportunity to work with our customers and the EU to reduce the environmental impact resulting from the continuous improvement of our products. -
Page 30: Revision History
Update sensitivity, range for phycocyanin (DCR 762) M. Johnson, H. Van Zee 9/14/11 Add WEEE statements (DCN 775) H. Van Zee 6/4/12 Add ESD operating notice (DCN 798) J. Pearson, H. Van Zee ECO FL User’s Guide (FL) Revision AN 4 June 2012...
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