Related Manuals for Sartorius mLINE
Summary of Contents for Sartorius mLINE
- Page 1 ® pipette User Manual Bedienungsanleitung Mode d'emploi Manual Usuario Instruzioni d'impiego Инструкция пользователя...
- Page 2 ® pipette User Manual ............1 Bedienungsanleitung .......... 17 Mode d’ emploi ............. 35 Manual Usario ............53 Instruzioni d’impiego .......... 71 Инструкция пользователя ......... 89 Specifications ............109 Huom! Suomenkielinen käyttöohje ladattavissa osoitteesta: www.sartorius.com Obs: Manual på svenska kan du ladda ner...
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Page 3: Table Of Contents
Contents 1. Intended use ..................... 2 2. Product description ..................2 3. Sartorius pipette tips ..................4 4. Unpacking ......................4 5. Personal identification .................. 5 6. Pipette holder and carousel stand ............5 6.1. Installing the pipette holder ............5 6.2. -
Page 4: Intended Use
Intended use mLINE pipette is intended to be used in liquid handling applications for dispensing liquids in volume range of 0,1 µl to 10 ml. It is recommended to use Optifit Tips or SafetySpace™ Filter Tips to ensure the best compatibility and performance with Sartorius pipettes. - Page 5 Pipette description Cap ....... ID window and tag Operating button ....ejector ..Finger support ..Handle Display ..... Volume ... Calibration lock ....Colour coding....Tip ejector collar ...... Tip cone....Safe-Cone ..Filter ..Tip cone housing Tip cone .......
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Page 6: Sartorius Pipette Tips
Safe-Cone Filters to help prevent the contamination and damage of the pipette. The operating button of the mLINE allows safe and easy filter ejection. The pipette uses disposable tips. Sartorius pipette tips Sartorius pipette tips are recommended for use with mLINE pipettes. -
Page 7: Personal Identification
5. Personal identification Each mLINE pipette can be identified with a tag located under the ID window which on the finger support. 1. Remove the ID window by using the calibration tool. 2. Remove the tag and mark the identifying information on it. -
Page 8: Operating The Pipette
8.3. Protective tip cone filters The tip cone design of the mLINE pipettes (>10 µl) allows the use of Safe-Cone Filters in the tip cones. These removable filters prevent liquids... -
Page 9: Pipetting Techniques
Pipetting techniques Two basic pipetting techniques, forward and reverse pipetting, are associated with mLINE pipettes. Forward pipetting is the most common used pipetting technique. The technique employs the blow-out function ensuring complete delivery of the liquid. -
Page 10: Repetitive Reverse Technique
liquid (2-3 mm) and smoothly release the operating button up to the starting position. 4. Withdraw the tip from the liquid touching against the edge of the container to remove excess. 5. Press the operating button smoothly to the first stop to deliver the desired volume. Hold the operating button at the first stop. -
Page 11: Maintenance
11.1. Daily cleaning of the outer surface of the pipette Your mLINE pipette should be checked every day for cleanliness. To clean and decontaminate the outer surface of your pipette use ethanol (70%), isopropanol (60%) or a mild detergent and a soft lint-free cloth. - Page 12 Sartorius service representative for cleaning and greasing. To clean and decontaminate the lower parts of the single channel pipette, follow these steps: Disassembling and cleaning (see the pictures under the cover page): 1. Eject the tip cone filter (if fitted, Chapter 8.3.) 2.
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Page 13: Sterilization Of The Pipette
11.3. Sterilization of the pipette The entire mLINE pipette can be sterilized by steam, autoclaving at 121°C, (252°F), 1 atm for Connecting 20 minutes. Remove the tip cone filter (if fitted, collar Chapter 8.3). -
Page 14: Testing The Performance
testing routine for their pipettes with regard to accuracy requirements of the application, frequency of use, number of operators using the pipette, nature of the liquid dispensed and the acceptable maximum permissible errors established by the user. (ISO 8655-1.) 12.1. Testing the performance Performance testing should take place in a draught-free room at 15 - 30°C, constant to +/- 0.5°C and humidity above 50%. -
Page 15: Recalibration
1.0036 1.0037 NOTE: This method is based on ISO 8655. 12.2. Recalibration The calibration of your mLINE pipette has been factory-checked by using forward pipetting technique and certified at 22°C using grade 3 distilled water according to ISO 3696. The... - Page 16 ISO 8655-6: Gravimetric test method for volumetric instruments. The pipette's specifications are guaranteed only with genuine Sartorius tips. If you find the pipette to be inaccurate after performance testing, please follow the instructions below: 1. Remove the lid of the calibration nut, located on the back of the handle, with the aid of the calibration tool.
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Page 17: Troubleshooting
13. Troubleshooting Symptom Possible cause Solution Droplets left Unsuitable tip Use original inside the tip Sartorius tips Leakage or pipetted Non uniform wetting Attach new tip volume too small of the plastic Tip incorrectly Attach firmly attached Unsuitable tip Use original... -
Page 18: Warranty Information
14. Warranty information The mLINE pipettes are covered by warranty for 2 years against defects in materials and workmanship. Should your mLINE pipette fail to function at any time, please contact your local Sartorius representative. ANY WARRANTY WILL, HOWEVER, BE DEEMED AS... - Page 19 Specifications mLINE single-, 8- and 12-channel pipettes Cat. No Channels Volume Test Inacc. (%) Impr. (%) Range µl Volume µl 725010 0.1-3 1.30 0.80 2.40 1.60 10.0 6.00 725020 0.5-10 1.00 0.60 1.50 1.00 2.50 1.50 725030 2-20 0.90 0.40 1.20...
- Page 20 The status of the information, specifications and illustrations in this manual is indicated by the date given aside. Sartorius reserves the right to make changes to the technology, features, specifications and design of the equipment without notice. All trademarks are Sartorius property unless otherwise stated.
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