Brunswick BIOFLO 3000 Manual To Operations
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Brunswick BIOFLO 3000 Manual To Operations

Brunswick BIOFLO 3000 Manual To Operations

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BIOFLO 3000
BENCH-TOP
FERMENTOR
MANUAL NO.: M1217-0050/K
Click here to view available Bioflo 3000 Bioreactor at
www.ssllc.com>>
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 . 44 TALMADGE ROAD . EDISON, NJ 08818-4005 . 732-287-1200
TELEPHONE: 800-631-5417 . FAX: 732-287-4222 . TELEX: 4753012 NBSCO
INTERNET: http://www.nbsc.com/ . E-MAIL: bioinfo@nbsc.com
M1217-0050
1

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Summary of Contents for Brunswick BIOFLO 3000

  • Page 1 BIOFLO 3000 BENCH-TOP FERMENTOR MANUAL NO.: M1217-0050/K Click here to view available Bioflo 3000 Bioreactor at www.ssllc.com>> NEW BRUNSWICK SCIENTIFIC CO., INC. BOX 4005 . 44 TALMADGE ROAD . EDISON, NJ 08818-4005 . 732-287-1200 TELEPHONE: 800-631-5417 . FAX: 732-287-4222 . TELEX: 4753012 NBSCO INTERNET: http://www.nbsc.com/ .
  • Page 2 M1217-0050...

  • Page 3: Table Of Contents

    3.12 Sampling Procedure 3.13 Shut-Down Procedures 3.14 Operation Tips MAINTENANCE General Console Cleaning Periodic Inspection Vessel & Tubing Cleaning Probe Maintenance & Storage The Agitator Bearing Housing Replacement Part List APPENDIX: Cell Culture on BioFlo 3000 LIST OF DRAWINGS M1217-0050...

  • Page 4: Scope Of Manual

    U.S.A.. DESCRIPTION OF EQUIPMENT BioFlo 3000 is a versatile bioreactor that provides a fully equipped fermentation and cell culture system in one compact package.* It can be employed for batch or continuous culture with microprocessor control of pH, DO2, agitation, temperature, pump feed, antifoam and vessel level.

  • Page 5: Ph Control

    PID controller which can change the speed of agitation and the percentage of oxygen in aeration. The D.O. probe used on BioFlo 3000 can be Phoenix polarographic or Ingold polarographic (Fig. 6, 7). FOAM CONTROL Foam is controlled during batch fermentation by the antifoam probe which is located in the headplate.

  • Page 6: Specifications

    See attached matrix. Voltage Universal 100V 120V 220V 240V There are five assignable peristaltic pumps on BioFlo 3000, namely, Feed 1, Feed 2, Feed 3, Feed 4 and Feed 5. Pumps Modes Operation Feed 1-5 Off, Manual, Base, Fixed Speed,...

  • Page 7: Operating Controls And Displays

    1.13 OPERATING CONTROLS AND DISPLAYS The main operating controls of the BioFlo 3000 is the membrane keypad. There are twenty keys on the keypad. The function of "Screen" key is to select the screen. There are three screens which can be selected on the display. The first one is the "Master" screen, the other two are: a "Calibration"...
  • Page 8 making these selections, move the cursor to the loop or the mode to be changed, then press the "Alter" key to get the desired selection and then press "Enter" key. If you have a loop set already, you cannot change into that loop. SCREEN <MASTER>...
  • Page 9 Calibration Value Zero Span Function 7.00 7.004.00 Read D.O. Read NOTE: 1. Change function to zero, calibrate zero. 2. Change function to span, calibrate span. Figure 16 There is a recorder output connector on the left hand side of the control cabinet. An external recorder with 0-1 volt high impedance input may be connected to the output.

  • Page 10: Inspection

    NOTE: See Fig. 24 for fuse replacing. INSTALLATION OF CONSOLE A. Position the BioFlo 3000 console on a firm level surface in an area where services are readily available. B. Level the horizontal surface of the base with four leveling glides if necessary.

  • Page 11: Computer Interface

    The RS232/422 Computer Interface is a 25 pin "D" type connector mounted on the left hand side of the electrical cabinet. It is by means of this connector that an external computer may communicate with and control the BioFlo 3000 Fermentor. Pin designations are as follows:...
  • Page 12 1200 2400 4800 * 9600 Switch positions 3 and 4 are used as follows: SW1-3 SW1-4 * Multidrop (8 data bits) and even parity check Multidrop (8 data bits) and no parity check No Multidrop (7 data bits) and even parity check No Multidrop (7 data bits) and no parity check OFF To select one of the sixteen addresses for the machine it is necessary to set the last four switches in accordance with the following table:...
  • Page 13 IMPORTANT NOTE: SW2-1 and SW2-2 have been set at "ON" position in the factory before shipping. If 1200 RPM agitation speed is required, please consult with New Brunswick Scientific Co. before taking action. M1217-0050...

  • Page 14: Cleaning Of Vessel

    CHAPTER 3 - PREPARATION & OPERATION (For preparation and operation of Cell Culture, please read CelliGen Plus Manual M1176-0050 - included with conversion kit - see p. 23) CLEANING OF VESSEL A. Fill the vessel with a mild detergent and water solution. Let stand for one hour then scour thoroughly.

  • Page 15: Ph Probe Preparation

    PROBE PREPARATION Inspect probe for possible shipping damage. If damage is observed notify the Service Department of the New Brunswick Scientific Co., immediately. Check the level of the reference electrolyte. It should be about 1cm below the filling orifice, which is closed with a rubber "T" stopper. To add reference electrolyte, take the filling pipette (P0740-4820) and fill it with Viscolyte B (P0860-0130) Electrolyte.

  • Page 16: Operation Of Dissolved Oxygen (Do) Probe

    NOTE: pH electrode is calibrated before autoclaving vessel. 1. Connect electrode to the pH connector using the appropriate cable. 2. Turn ON the main power switch. 3. Display calibration screen. NOTE: The pH measuring system is calibrated using two external buffer solution of known pH.
  • Page 17 sampler valve. Sterilize the complete assembly consisting of jar, headplate and components of headplate by inserting in an autoclave. NOTE: For continuous culture, vessel should not be sterilized empty. Use at least 100 mL of sterilized water. Probe tips must be moist during sterilization. After autoclaving medium will be brought from the medium reservoir, sterilized separately.

  • Page 18: Dissolved Oxygen Electrode (D.o.) Calibration

    O2 amplifier for polarization purpose prior to calibration. The electrode is polarized and ready for operation after six hours of polarization time. 1. There are two methods of obtaining zero on the BioFlo 3000. Use either Method 1 or Method 2. Method 1 Remove the D.O.

  • Page 19: Cascade System

    C. Add glycerin to the thermowell and insert (RTD) temperature probe. D. Turn on the water valve, adjust water pressure to 20 PSI. E. Connect air and O2 to the unit, adjust gas pressure to 10 PSI. CAUTION: NEVER ATTACH TUBINGS MADE OF HARD MATERIAL, SUCH AS TEFLON, DIRECTLY TO THE PLASTIC HOSE BARBS ON THE GAS ENTRY.

  • Page 20: Sampling Procedure

    3.11.1 Agitation/D.O. Cascade System To set up, proceed as follows: Set agitation and D.O. loop to PID mode. b. Set agitation to the minimum RPM you wish for D.O. control. Change agitation mode to D.O.. d. Set agitation speed to maximum RPM you wish for D.O. control. D.O.

  • Page 21: Operation Tips

    2. Set the agitation, temperature to "OFF". 3. Turn off the power. 4. If the system is not to be used for several days, disconnect power plug. Remove and clean vessel, and associated components as outlined in previous steps. NOTE: DO NOT WASH THE FILTERS OR GET THEM WET. 3.14 OPERATION TIPS A.
  • Page 22 Operate machine at 37C, 1000 RPM, air flow = (1/2 - 1 V) LPM. Calibrate DO to 100 and allow it to stabilize. Add Na2SO3 (with a small amount about 5gm of Cu SO4). The DO reading drops very quickly. At DO = 50, start counting time. When DO returns to 50, stop counting time.

  • Page 23: General

    CHAPTER 4 - MAINTENANCE GENERAL Preventive maintenance is performed to keep equipment in proper working condition. When periodically performed, it will result in longer life for the equipment and reduce time lost due to equipment failure. CONSOLE CLEANING At least once a month, clean all metal parts of unit. Use a damp cloth moistened with water or mild detergent.

  • Page 24: The Agitator Bearing Housing

    Check the level of the filling solution. It should be about 1cm below the filling orifice. To add solution, see section 3.3. The probe should be stored standing upright. The electrode tip should be immersed in a solution of 3-Molar KCL or a Buffer Solution between pH 4 and pH 7. The two rubber "T"...
  • Page 25 Glass Vessel 5L M1155-9904 Fuse, 10 Amp, Motor Drive P0380-3160 Bearing Housing Cover M1151-9444 Bearing Housing Cover O-Ring P0280-6093 Motor Assy. (1 1/4, 2 1/2 & 5L) M1169-0600 Flat Gasket, Vessel (1.25L) M1151-9905 Flat Gasket, Vessel (2.5L) M1152-9904 Flat Gasket, Vessel (5L) M1155-9902 Ball Bearing P0180-0170...

  • Page 26: Appendix Cell Culture On Bioflo 3000

    APPENDIX CELL CULTURE ON BIOFLO 3000 When BioFlo 3000 is used for cell culture, the following items should be prepared. A. Vessel Assembly (Basic) NBS Part No. Total Volume M1176-0006 2.2L M1176-0008 M1176-0003 7.5L B. One of the following items should be selected.
  • Page 27 NBS Part No. Total Volume M1176-0115 2.2L M1176-0213 M1176-0215 7.5L D. One of the following Perfusion Kits could be selected. 1. Perfusion Kit for Cell Lift Application. NBS Part No. Voltage M1176-0120 - Plus optional pump P0620-0485 110/120V M1176-0120 - Plus optional pump P0620-0486 220/240V 2.
  • Page 28 LIST OF DRAWINGS FIGURE 1 VESSEL ASSEMBLY FIGURE 2 HEADPLATE ARRANGEMENT FIGURE 3 THERMOWELL INSTALLATION FIGURE 4 GAS DELIVERY SYSTEM FIGURE 5 pH PROBE MOUNTING FIGURE 6 D.O. PROBE MOUNTING (Phoenix Probe) FIGURE 7 D.O. PROBE MOUNTING (Ingold Probe) FIGURE 8 FOAM PROBE FIGURE 9 EXHAUST CONDENSER...

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