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Andor Technology Optosplit II User Manual

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Optosplit II
Image Splitter
User Guide v3.1

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Summary of Contents for Andor Technology Optosplit II

  • Page 1 Optosplit II Image Splitter User Guide v3.1...

  • Page 3: Important - Please Read Before Installation

    Important - Please read before installation Important - Please read before installation For maximum reliability we recommend using the equipment within certain guidelines. If in any doubt, then please feel free to contact our technical support department (e-mail tech@cairn- research.co.uk). The following points should be considered when using the Optosplit for the first time: 1.

  • Page 5: Package Contents

    Package Contents II.Package Contents Optosplit Image Splitter Optosplit Body  Filter Cube(s) ____________  2 slot covers  Shutter to occlude either optical path  Auxillary filter/lens holder  Coupling Rectangular diaphragm with female c mount input  Output focussing adjuster with male c mount output ...

  • Page 6: Table Of Contents

    III.Table of Contents Important - Please read before installation..................i II. Package Contents........................... iii III. Table of Contents........................... v Introduction............................. 1 Installation guide............................. 3 2.1 Configuring the filter and dichroic mirror holder..............4 2.2 Installing the Optosplit in the light path................5 2.3 Focussing the camera.

  • Page 7: Table Of Contents

    Table of Contents Page v...

  • Page 9: Introduction

    Introduction Introduction In fluorescence imaging applications it is often useful to acquire simultaneous images at two different emission wavelengths. Traditionally such applications have been restricted by the speed of an electronic filter changer, or by the cost and complexity of adding a second camera to a system. In many instances the region of interest does not require the full resolution of the camera so the ideal solution would be to simultaneously image at two different wavelengths on the same camera chip.

  • Page 11: Installation Guide

    Installation guide Installation guide The Optosplit comes supplied with a rectangular aperture, as shown below. This allows you to make the best use of the camera chip area, but there is the alternative of a circular iris available. Figure 1 : Optosplit with adjustable rectangular diaphragm Before installing the Optosplit you will first need to install an appropriate filter cube into the filter holder.

  • Page 12: Configuring The Filter And Dichroic Mirror Holder

    2.1 Installing the filter cube The first stage in configuring the Optosplit is to install the filter set. If you wish to change the configuration after installation then the instructions here should be followed, but it is not necessary to remove the Optosplit from the light path.

  • Page 13: Installing The Optosplit In The Light Path

    Installation guide Note: To control the relative intensity of the two pathways we recommend using neutral density filtering in the brighter pathway using the auxilliary component mounts (M in fig 2)This is neccesary only when one image is disproportinately brighter than the other. Figure 4 : Filter cube in place 2.2 Installing the Optosplit in the light path...

  • Page 14: Focussing The Camera

    2.3 Focussing the camera on the Optosplit aperture. Figure 6 : Adjustments to focus the image on the camera If the Optosplit aperture is not in sharp focus on the camera, then adjust the fine focus on the camera using the following procedure. Refer to fig.6 above for screw locations: 1.

  • Page 15: Operation Of The Optosplit

    Operation of the Optosplit Operation of the Optosplit The Optosplit is an intuitive device, with a single control for adjusting image separation (blue arrow in fig 7) to allow different sized samples to be used. There are additional controls for defining the field of view, and image centering which depend on the type of aperture fitted to the Optosplit.
  • Page 16 Separating the two Images Turning the horizontal image separation adjustment counterclockwise will split the two images (fig.7 blue arrow). Note: Only small adjustments are necessary to separate the images. Figure 9 : Image separation Adjust vertical position of short wavelength image Should the left and right images be at different vertical heights, this can be corrected by adjusting the position of the short wavelength image.

  • Page 17: Configuring A New Filter Set

    Operation of the Optosplit 3.2 Centering the Optosplit images on the camera When the Optosplit is initially installed, or if the filter block goes out of alignment, the Optosplit images can show significant aberrations, or in extreme cases only a single image may be observable for any setting of the separation control.

  • Page 19: Technical Summary

    Technical summary Technical summary The Optosplit uses a conventional dichroic mirror to separate the light into two different spectral bands. This selection of wavelength is usually augmented by the use of bandpass filters (as shown in the schematic below.) The design is loosely based on technology described by Kinosita et al. (J.Cell Biol.(1991) 115, 67-73), but includes several proprietary features, most notably: •...

  • Page 21: Technical Support

    Technical Support Technical Support e-mail : tech@cairn-research.co.uk Web : http://www.cairn-research..co.uk/ Address : Cairn Research Ltd Graveney Road Faversham Kent ME13 8UP Telephone : +44 (0) 1795 590 140 Fax : +44 (0) 1795 594 510 Page 13...

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