Toyota prius Owner's Manual page 519

Taking out the spare tire
CAUTION
 Service plug
Be careful not to hit the service plug when loading or unloading the tool
holder or the spare tire. (P. 45)
PRIUS_OM_OM47871E_(EE)
5-2. Steps to take in an emergency
Loosen the center fastener that
secures the spare tire.
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