Asus P5B Premium Series Hardware User Manual page 50

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2-22
We recommend that you install a graphics card on the primary (blue)
PCI Express slot and install other PCI Express devices on the black PCI
Express x16 slot.
In CrossFire™ mode, install the ATI CrossFire™ Edition (Master) graphics
card on the primary (blue) PCI Express slot.
Install two ATI graphics cards from the same GPU family that support
CrossFire™ mode.
Chapter 2: Hardware information