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BX3-URA
BX3-RFAS
U-LH100HGAPO
U-LH100HG
U-RFL-T
U-AN-2
BX3-25ND6
BX3-25ND25
This instruction manual is for the Olympus Reflected Fluorescence System. To ensure the safety,
obtain optimum performance and to familiarize yourself fully with the use of this system, we
recommend that you study this manual thoroughly before operating the microscope. Retain this
instruction manual in an easily accessible place near the work desk for future reference.
INSTRUCTIONS
REFLECTED
FLUORESCENCE
SYSTEM
A X 7 8 8 8
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Related Manuals for Olympus BX3-URA
Summary of Contents for Olympus BX3-URA
- Page 1 FLUORESCENCE SYSTEM This instruction manual is for the Olympus Reflected Fluorescence System. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this system, we recommend that you study this manual thoroughly before operating the microscope. Retain this instruction manual in an easily accessible place near the work desk for future reference.
- Page 2 This device complies with the requirements of both directive 2004/108/EC concerning electromagnetic compatibility and directive 2006/95/EC concerning low voltage. The CE marking indicates compliance with the above directives.
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Page 3: Table Of Contents
Centering the Field Iris Diaphragm..........................11 Centering the Aperture Iris Diaphragm .........................12 Centering the Mercury Burner ............................13 · BX3-RFAS · BX3-URA Mounting the ND Filters ................................16 Attaching the Light Shield Sheet ...........................17 SIMULTANEOUS FLUORESCENCE OBSERVATIONS Simultaneous Reflected Fluorescence and Phase Contrast Observations ......18 Simultaneous Reflected Fluorescence and Transmitted Light Nomarski Differential Interference Contrast (DIC) Observations ..................18... -
Page 4: Important
6. Do not attempt to open, disassemble or modify the power supply unit because it includes high voltage parts inside. 7. Always use the power cord provided by Olympus. If no power cord is provided, please select the proper power cord by referring to the section “PROPER SELECTION OF THE POWER SUPPLY CORD”... -
Page 5: Safety Symbols
Caution indications are placed at parts where special precaution is required when handling and using the System. Always heed the warnings. · Lamp housing for mercury burner (U-LH100HG, U-LH100HGAPO) [Caution against · Illuminator high temperature] (BX3-URA, BX3-RFAS) Caution indication · ND filter high voltage] position (BX3-25ND6, BX3-25ND25) · ND filter slider [Caution against... -
Page 6: Maintenance And Storage
6. When disposing of the system, always follow your local regulations and rules. If you have any question, contact Olympus. Caution If the system is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the system equipment may also be damaged. -
Page 7: Nomenclature
NOMENCLATURE Universal Reflected Illuminator Apo Lamp Housing for 100 W Mercury Burner U-LH100HGAPO BX3-URA Lamp Housing for 100 W Mercury Burner U-LH100HG Field iris diaphragm centering screws (Page 11) x2 screws. Collector lens focusing knob (Page 13, 1 4) Burner centering knobs... - Page 8 Coded Fluorescence Illuminator BX3-RFAS Mirror unit inscription pockets (Page 25) ND filter slider insertion slot (Page 16) Mirror unit turret Insertion from right side. Aperture iris diaphragm knob (Page 12) Field iris diaphragm knob (Page 11) Field iris diaphragm centering screws (Page 11) Shutter knobs (Provided on both sides.) (Page 11) Analyzer inlet...
- Page 9 } Up to eight fluorescence mirror units can be mounted on the BX3-URA Fluorescence Mirror Units or BX3-RFAS. } It is recommended that you use the U-FF dummy mirror unit (which does not contain a filter) when making your original fluorescence mirror unit (page 27).
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Page 10: Reflected Fluorescence Observation Procedure
5Revolving nosepiece on the specimen 6Coarse/fine adjustment knobs Engage an ND filter in the 7ND filter (P.16) light path as required. BX3-URA BX3-RFAS Adjust so that the Adjust the brightness entire field is uniform 8Collector lens focusing knob (P.13, 1 4) as required. - Page 11 } Make a photocopy of the observation procedure pages and post it near your microscope.
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Page 12: Using The Controls
The turret must always be set to one of the click position. If it is deviated from a click position, the cover may be deformed by heat. 3. Always use Olympus immersion oil for oil immersion objectives. 4. If you use an objective with correction collar, you can correct contrast degradation due to variation in cover glass thickness by adjusting the correction collar. - Page 13 Dichroic Mirror and Filter Configurations of Fluorescence Mirror Units Excitation Mirror unit Dichroic mirror Excitation filter Barrier filter Applications method · Autofluorescence observation U-FUW BP340-390 DM410 BA420IF · DAPI: DNA staining U-FUN BP360-370 · Hoechest 33258, 33342: Chromosome · Catecholamine observation U-FVN DM455 BP400-410...
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Page 14: Turning The Power Supply Unit On
Turning the Power Supply Unit On Set the main switch to “ I ” (ON). The illumination light will stop flickering and will stabilize in 5 to 10 minutes after ignition. } The discharge type mercury burner may not be ignited from the beginning on rare occasions due to its characteristics. In this case, set the main switch to “... -
Page 15: Centering The Aperture Iris Diaphragm
Centering the Aperture Iris Diaphragm (BX3-URA) (Fig. 2) } This operation is not required with the BX3-RFAS code fluorescence illuminator 1. Close the light path by sliding the shutter knob @ to position marked {. 2. Engage a mirror unit other than the ones for U-excitation (U-FUW, U-FUN, U-FUNA) in the light path by rotating the turret. -
Page 16: Centering The Mercury Burner
Centering the Mercury Burner The mercury burner emits light by means of discharge produced when a current is supplied across the poles. If the positions of the poles are moved for example during replacement of the burner, the light would be improperly incident to the specimen and the observation image becomes dark. -
Page 17: Bx3-Ura
· Operation with BX3-URA When the illuminator is the BX3-URA, project the lighting section between the poles (arc image) on the stage and adjust the positions of the poles while observing the arc image position. } Set the main switch of the power supply unit for lamp housing to “ I ” (ON) and wait until the illumination light stops flickering and its brightness is stabilized. - Page 18 Precise Centering of the Mirror (Fig. 8) } The mirror position has been adjusted and fixed at the factory. When you want to make your adjustments very strict and precise, perform the following procedure after completing the mercury burner centering adjustment described on the previous pages.
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Page 19: Mounting The Nd Filters
To switch the ND filter position, hold the ND filter slider by the top and bottom edges and slide it. Operation with BX3-URA (Fig. 9) 1. Insert the filters required for the observation (BX3-25ND6, BX3-25ND25) 3 into the two ND filter insertion slots @ 2 Up to two filters can be inserted from both sides. -
Page 20: Attaching The Light Shield Sheet
Attaching the Light Shield Sheet } When a low-power objective is used in fluorescence observation, the fluorescence image may be degraded by the reflected light from the condenser and the area around it. In this case, use the light shield sheet. -
Page 21: Simultaneous Fluorescence Observations
SIMULTANEOUS FLUORESCENCE OBSERVATIONS } By properly combining equipment, this system can be used in transmitted light brightfield observation, transmitted phase contrast observation and transmitted light DIC observation in addition to the reflected fluorescence observation. With specimens that fade rapidly, fading can be minimized by initially using transmitted light phase contrast or transmitted light DIC observation for positioning. -
Page 22: Troubleshooting Guide
Field contains dark, spot-like areas. Dirt or dust on burner or on burner side Clean them. of collector lens. 2. Electrical system . If the following remedy does not solve the problem, please contact Olympus. a) Main switch cannot turn system ON. Power cord is not connected properly. Connect firmly. -
Page 23: Spectral Characteristics Of Filters
SPECTRAL CHARACTERISTICS OF FILTERS 1. U-excitation (Wide band) 5. B-excitation (Wide band) U-FUW U-FBW Wavelength (nm) Wavelength (nm) 2. U-excitation (Narrow band) 6. B-excitation (Narrow band) U-FUN U-FBN Wavelength (nm) Wavelength (nm) 3. V-excitation (Narrow band) 7. G-excitation (Wide band) U-FVN U-FGW Wavelength (nm) - Page 24 9. U-excitation, color separation (Narrow band) 13. G-excitation, color separation (Narrow band) U-FUNA U-FGNA Wavelength (nm) Wavelength (nm) 10. IB excitation, color separation (Wide band) 14. For cyan fluorescent protein (CFP) U-FBWA U-FCFP Wavelength (nm) Wavelength (nm) 11. IB excitation, color separation (Narrow band) 15.
- Page 25 17. For red fluorescent protein (RFP) 18. For mCherry U-FRFP U-FMCHE Wavelength (nm) Wavelength (nm) Typical Example of Emission Spectrum of Ultrahigh-Vacuum Mercury Burner Spectrum Wavelength (nm) For fluorochrome emission, a light beam having a specific wavelength is selected from a wide spectrum of wavelengths.
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Page 26: Specifications
Lamp housing for mercury burner · Lamp housing for 100 W mercury burner U-LH100HG · Apo lamp housing for 100 W mercury U-LH100HGAPO · Mercury burner: USH-103OL (OLYMPUS) · Power supply unit U-RFL-T Mercury burner · DC, 100 W, ultrahigh-pressure mercury burner ·... -
Page 27: Assembly
The module numbers shown in the following diagram are merely the typical examples. For the modules with which the module numbers are not given, please consult your Olympus representative or the catalogues. When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching CAUTION any parts or touching glass surfaces. -
Page 28: 8-2 Detailed Assembly Procedures
8-2 Detailed Assembly Procedures Attaching the Fluorescence Mirror Units (Figs. 12 to 15) 1. Hold the left and right sides of the cover @ on the front of the illuminator and remove the cover by pulling it toward you. 2. Rotate the turret 3 so that the turret position 2 you want to mount the fluorescence mirror unit comes on the front. - Page 29 4. Attach the + (positive) pole of the mercury burner | to the fixed mount on the upper side, then the − (negative) pole to the mount on the lower side. · Be sure to use the USH-103OL (mfd, by OLYMPUS) burner. CAUTION · Be careful and avoid leaving fingerprints or contaminants on the mercury burner.
- Page 30 Making an Optional Fluorescence Mirror Unit } You can also fabricate original fluorescence mirror units by fitting a commercially available barrier filter, excitation filter or dichroic mirror in the U-FF empty mirror unit frame. Dimensions of Optical Parts · Barrier filter Diameter 25 -0.1/-0.2 mm, max.
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Page 31: Lamp Housing Inspection Sheet
{ If there is any ( ) mark noted, immediately stop use of the product, and contact Olympus for detailed inspections or replace the lamp housing. { If you detect an abnormality other than that listed below or with other Olympus product, also stop the use of the product and contact Olympus for detailed inspections. -
Page 32: Proper Selection Of The Power Supply Cord
If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and “ Certified Cord ” below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the equipment. - Page 33 Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Alternative Marking Utilizing Printed or Embossed Harmoni- Black-Red-Yellow Thread (Length zation Marking (May be located of color section in mm) Approval Organization on jacket or insulation of internal wiring) Black Yellow...
- Page 34 MEMO...
- Page 36 M-03...