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Related Manuals for NanoEnTek Adam rWBC2
Summary of Contents for NanoEnTek Adam rWBC2
- Page 1 Instruction Manual ADAM-rWBC2, residual Leukocyte Counter...
- Page 2 Fax. +82-2-6220-7999 The information in this manual is described as accurately as possible. Firmware and software changes and updates may change without prior consent or notification. Copyright ©2018 by NanoEntek Inc. All rights reserved. Published in Korea. Protected by patents US 7,842,157;...
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Page 3: Table Of Contents
Table of Contents Introduction General Description Technology - Mechanical Technology - residual Leukocytes Counting Product Description 7-10 Packing List Front View of ADAM-rWBC2 Rear View of ADAM-rWBC2 System Installation Environmental Requirements Power On and Initial Display General Operation 13-21 Calibration Sample Preparation and Testing Run Sample Result Analysis... -
Page 4: Introduction
Introduction ADAM-rWBC2 is an analyzing device that counts the number of residual leukocytes in a General blood component for transfusion. Targeting the leukocyte, ADAM-rWBC2 uses fluorescent Description dye to perform cell dyeing and automated cell counting. Automated cell counting elimi- nates user bias or subjective interpretation that can be found when counting residual leu- kocytes using other methods. -
Page 5: Technology - Mechanical
(4) interpretation may be subjective. Other devices that count residual leukocytes can be costly and very user technique dependent. NanoEntek has developed ADAM-rWBC2, which is based on fluorescent microscopy techniques for counting cells. ADAM-rWBC2 utilizes sensitive fluorescence dye stain- ing, LED optics and CMOS detection to make provide accurate and reliable leukocyte detection. -
Page 6: Technology - Residual Leukocytes Counting
Introduction ADAM-rWBC2 is based on the staining of mammalian cell DNA using the fluorescent dye, Technology Propidium Iodide (PI), a nucleic acid dye. When used with RNase, PI stains only cellular - residual Leukocytes DNA. In the case of blood samples, white blood cells (with DNA-containing nuclei) are stained while platelets, red blood cells and plasma (without nuclei) are not stained. -
Page 7: Product Description
ADAM-rWBC2 is shipped with the components listed in the table below. Once you receive Packing List your instrument, please check that all items listed below were shipped. If any items are missing or damaged, contact your local distributor or ivdst@nanoentek.com. Quantity Item... - Page 8 Product Description Packing list Adapter & Power cord Main device ADAM-rWBC Kit Installation USB USB cable (Starter kit with package insert) Cat. No. AD1K-050 Instruction manual ADAM-rWBC2 PC Software User Manual Optional Quick manual Barcode Scanner...
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Page 9: Front View Of Adam-Rwbc2
Product Description Door Power Front View of ADAM-rWBC2 Description Control buttons Ejects the slide holder from ADAM-rWBC2. EJECT Functions as unload. RUN SAMPLE Performs all procedures of automatic counting. Protects the alignment of stage from external shock LOCK when ADAM-rWBC2 is being moved. Lock ADAM-rWBC2 before turning it off or moving it. -
Page 10: Rear View Of Adam-Rwbc2
Product Description Rear View of ADAM-rWBC2 USB Port PC port Power plug Description Port USB Port Port for software and firmware updates Connects USB cable to ADAM-rWBC2 and PC for PC port using a software Power Plug Connects ADAM-rWBC2 power cord to wall outlet... -
Page 11: System Installation
2. Press the power button for 2~3 seconds. Initial Display If you get an error message, please contact your local distributor or ivdst@nanoentek.com. If booting is successful and no errors are detected, the home screens will be displayed as below. - Page 12 Initial Display [System State] It appears when booting is not working properly. Turn off main power and restart device. If this message still appears after restarting, contact your local distributor or ivdst@nanoentek.com. Cause Error code 0x00000C00 Failure of X-axis sensor...
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Page 13: General Operation
General Operation Calibration test Calibration Prior to use ADAM-rWBC2, calibration test MUST be performed. The calibration test should be performed to verify if optic and auxiliary structures of ADAM-rWBC2 are com- pletely ready for the real sample test by using Standard bead solution provided in ADAM- rWBC kit. - Page 14 General Operation Please check the procedure of sample preparation and testing below. Sample Preparation For more detailed information, please refer to the next page. and Testing r-Solution Sample 1. Mix sample with r-Solution & Load the mixed sample (100 μL) 2.
- Page 15 General Operation Sample Preparation 1. Leave the ADAM-rWBC Kit at room temperature for 10 minutes before use. and Testing 2. Carefully dispense 100 µL of well-mixed RBC or platelet or plasma sample into a clean test tube. 3. Add 400 µL of reagent into the tube. 4.
- Page 16 General Operation WARNING Sample Preparation [Sample loading error] and Testing Be cautious of loading the correct volume(100 μL) of the sample into r-Slide. The instrument will not detect low or high sample volumes. CAUTION Avoid bubbles which may negatively affect the result. Correct volume Low volume...
- Page 17 General Operation WARNING Sample Preparation [r-Slide insert error] and Testing Completely insert r-Slides face up, in the direction of the arrow on the slide. The instrument will not detect if slides are inserted incorrectly. See pictures below for proper insertion. ( O ) ( X ) Correctly inserted...
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Page 18: Run Sample
General Operation Start counting process by pressing RUN SAMPLE Run Sample It may take about 1 minute longer for auto focus at the initial test. Note: If the ADAM-rWBC2 instrument is not connected to the PC software, the pop-up window will appear as below. Select the Sample type and press the OK button to proceed with the counting process. -
Page 19: Result Analysis
General Operation The total number of leukocytes will be displayed after being automatically calculated by Result Analysis ADAM-rWBC2 software. 1.71/μL Total Number of Leukocytes Error Message [Bad Sample] 50.58 When 1 cell including dust consists of more than 100 pixels or the number of cells is more than 160 in 1 frame, this is considered as error frame. -
Page 20: Lock
General Operation Press LOCK before turning off the device. Lock If there is no operation for 1 minutes, the lock function will be activated automatically. When the device is locked, the screen will be changed as shown below. If you press the power button for 2~3 seconds, then ‘Slide to shut down your PC’ message Power Off will appear. -
Page 21: Maintenance And Cleaning
General Operation ADAM-rWBC2 does not need regularly scheduled maintenance because ADAM-rWBC2 Maintenance has no consumable materials to replace. and Cleaning Please clean the exposed surface of ADAM-rWBC2 frequently or before testing, using a soft cloth and isopropyl alcohol or deionized water. ADAM-rWBC2, residual Leukocyte Counter... -
Page 22: Trouble Shooting
Trouble Shooting Problem Cause Solution ADAM-rWBC2 does not power up No power from outlet Check power source. Bad power cord Replace or Repurchase. LED on the power button blinks and Unplug the adapter and plug it back in Problem with Windows OS the screen does not turn on to power on the device. -
Page 23: Warranty
Warranty If any defects occur in the ADAM-rWBC2 during one(1) year warranty period, NanoEntek will repair or replace the defective parts at its discretion without charge. The following defects, however, are specifically excluded: Defects caused by improper operation. Repair or modification done by anyone other than NanoEntek or an authorized agent. -
Page 24: Technical Specifications
Technical Specifications ADAM-rWBC2 Measuring range 1~100 cells /μL Analysis time < 1 min /test Voltage DC12V Current Objective lens 4W Green LED Camera CMOS camera Filter Excitation filter, Dichroic filter, Emission filter Weight 7 Kg Size (W×L×H) 227 × 276 × 270 mm Degree of protection IPX0 Operating environment condition... -
Page 25: Product List
Standard Bead Solution 7ml Instruction manual * Each component and power cord are available for purchase separately. Please contact your local distributor or ivdst@nanoentek.com. * This Instruction Manual can be downloaded from the NanoEntek website, www.nanoentek.com. ADAM-rWBC2, residual Leukocyte Counter... -
Page 26: Safety Precautions
Safety Precautions Review and follow the safety instructions below : • If water or other material enters the instrument, the adaptor, or power inlet, disconnect the power cord and contact a service person. For operating environment, refer to Product Specifications. •... - Page 27 Consignes de securite Examinez et suivez les consignes de securite ci-dessous : • Si de l'eau ou d'autres matières entrent dans l'instrument, l'adaptateur, ou l‘entrée de la prise, débrancher le cordon d'alimentation et contacter un technicien de service. Pour l'environnement d'exploitation, se reporter aux Spécifications du Produit.
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Page 28: Safety Symbols
Safety Symbols The following symbols are found on the instrument and this document. Al- ways use the equipment in the safest possible manner. Symbol Meaning Caution, warning, Consul accompanting documents Catalogue number/Reference number Consult Instructions for Use An electronic instructions for use (eIFU) indicator (website address) may accompany the symbol when used to indicate an instruction to consult an eIFU. - Page 29 Safety Symbols The following symbols are found on the instrument and this document. Al- ways use the equipment in the safest possible manner. Symbol Meaning For prescription use only CAUTION: Federal (The U.S.) law restricts this device to sale by or on order of a physician. This product conforms to UL 61010-1, CAN/CSA C22.2 No.61010-1 “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part l: General Requirements.
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Page 30: Warnings
Warnings 1. After using device, please turn off main power. If not, it can be cause of malfunction or reduce lifetime of device. 2. When turn off the device, be sure lock the device with Lock button. If not, it can be cause of mechanical problem or error message when device is booting. Item Warning Cover... -
Page 31: Technical Support
Technical Support Visit the our Website at www.nanoentek.com for : · Technical resources, including manuals, FAQs, etc. · Technical support contact information · Additional product information and special offers. For more information or technical assistance, please call or email. NanoEntek, Inc. - Page 32 NanoEntek, Inc. 851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea Tel : +82-2-6220-7940 / Fax : +82-2-6220-7999 NanoEntek America, Inc. 220 Bear Hill Road, Suite 102, Waltham, MA 02451, USA Tel : +1-781-472-2558 / Fax : +1-781-790-564 MT Promedt Consulting GmbH.