Related Manuals for NanoEnTek ADAM-MC
Summary of Contents for NanoEnTek ADAM-MC
- Page 1 ADAM-MC A New Standard of Automated Cell Counter USER MANUAL NESMU-AMC-001E (V.6.1)
- Page 2 The information in this manual is described as correctly as possible and is applicable to the latest firmware and software versions, but it may be changed without prior consent or notification. Copyright 2008, by NanoEntek, Inc. All rights reserved. Published in Korea. Documentation : NESMU-AMC-001E (V.6.1) Revision History : V.0.0...
-
Page 3: Table Of Contents
Result analysis Error code Maintenance and cleaning Software installation 26-33 Introduction Getting started Installation 27-28 Function guide Function buttons Data list Information Graph Sample image of error message Trouble shooting Warranty Product list ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc. -
Page 4: Product Contents
Product Contents The contents of the ADAM-MC are listed below: ADAM-MC Item Quantity Main device User manual USB Cable Installation CD Key Pad Power Cord Fuse External video monitor (Optional) The contents of the ADAM AccuChip Kit are listed below :... -
Page 5: Safety Information
12. Use this equipment only as specified in this manual and as specified in any documentation associated with its components. Any use of the equipment in an unspecified manner is strongly discouraged and may result in damage or injury as cautioned by signed warnings. ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc. - Page 6 Safety information The symbols used in the ADAM-MC and the manual are Safety explained below: symbols Symbol Meaning The Caution symbol denotes a risk of safety hazard. ON (Power) Protective earth (Ground) The CE mark symbolizes that the product conforms to all applicable European Community provisions for which this marking is required.
- Page 7 · After using AccuChips, appropriately dispose it as biohazardous waste. · Do not reuse the AccuChips. Operator · Must have the general knowledge of cell counting procedure and bio safety to handle the sample that may have the infectious biohazardous substance. ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc.
-
Page 8: Product Specifications
Product specifications ADAM-MC Voltage 100-240V~, 50/60 Hz Current max. 1.8A, max 100W Fuse F3.15AL250V Objective lens Green LED CCD camera B/W CCD Filter Excitation filter Dichroic filter Emission filter Weight 9 kg Size (W × L × H) 220 × 375 × 250 mm... -
Page 9: Description Of Adam-Mc
The ADAM-MC, an automated cellcounter, is a benchtop automated Front view of cell counter that performs cell count and viability measurements ADAM-MC using AccuStain solution. The front view showing various parts of the ADAM-MC is shown below: Control Button Door Key Pad Control buttons Eject : Ejects the chip holder from the ADAM-MC. - Page 10 Description of ADAM-MC The rear view showing various parts of the ADAM-MC. Front view of ADAM-MC Key Pad Port RS232C Port Power Switch USB Port Power Plug Video Port Fan : Cooling fan Power switch : Main power on/off switch...
-
Page 11: Introduction
LED optics and CCD detection technologies to make the cell analysis more accurate and reliable. To count cells using ADAM-MC, the cells are mixed with a Propidium Iodide (PI) stain and directly pipettedon to a disposable plastic chip. The chip is then loaded onto a precision stage. -
Page 12: Basic Principals Of Counting
Introduction ADAM-MC is based on staining mammalian cell DNA with a Basic fluorescent dye, Propidium Iodide (PI). PI does not enter cells principals of with intact cell membranes or active metabolism. In contrast, cells with damaged membranes or with inactive metabolism are counting unable to prevent PI entering the cell. -
Page 13: Getting Started
1. Room temperature between 20 and 35 °C. · Not recommended for cold room use (≤ 4 °C). · At low temperature (≤10 °C), warm up the ADAM-MC for 10 min. 2. Not exposed to direct sun light. 3. Not subject to direct or continuous vibration. -
Page 14: Installation
Getting started 1. Remove all components of ADAM-MC from their box. Installation 2. Place the instrument in a flat level and dry surface. 3. Plug to power cord into the electrical outlet. · Be sure to use only the power cord supplied with your instrument. -
Page 15: Start-Up Screen
2. System will go through self diagnostic tests. · If you get an error message, please contact your local distributor or sales@nanoentek.com 3. The home screens will be displayed as the image, no errors are detected. ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc. -
Page 16: Error Message During Booting
3. If this message still appears after restart, contact your local distributor or sales@nanoentek.com 1. It appears when a chip is inserted during start up. 2. Remove the chip from a device, and do not turn on the device with a chip. -
Page 17: Menu Setting
‘Enter’ key. [System Info.] Version : MC V1.21 3. The screen will return to Date : Sep 23, 11 the menu screen auto- CORE1 : 1.87 CORE2 : 1.52 matically. CORE3 : 1.53 ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc. -
Page 18: Chip Selection
Getting started 1. Press ‘1’ for chip selection. Chip · You can select the number selection from the menu. 2. User can select the 2 kinds of chip type. ‘1’ : Two channel chip · (AccuChip2X ) ‘2’ : Four channel chip ·... -
Page 19: Counting Method
High Precision : 22 frames capture High Speed : 11 frames capture 3. Press ‘1’ or ‘2’ and ‘Enter’ key. 4. After pressing the ‘Enter’ key, the screen will return to the menu screen auto- matically. ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc. -
Page 20: General Operation
Instruction is provided in this section for preparing the cell Introduction sample with AccuStain solution for use with disposable AccuChip for automated cell count using the ADAM-MC. 1. Cultivate the required number of cells. Preparing 2. Add an appropriate volume of growth media or PBS to dilute... -
Page 21: Operating Adam-Mc
☞ Note :Automatic focus will be carried out at the first time the device is booted. Once ADAM-MC has done the auto focus process and on the following time,focusing process will be skipped. 4. The instrument takes approximately 90 seconds to count sample. -
Page 22: Icon Function
Insert (After pressing the Eject button with AccuChip). 2. Display menu setting Shows that performance setting is high precision. ADAM-MC scans 22 fields in each chamber, representing a total volume of 3.1 μL. Shows that ADAM-MC reads 4 Channel chip. -
Page 23: Result Analysis
N1 (5.50E5) T2 (2.20E6) N2 (5.50E5) · The viability will be automatically calculated by the ADAM-MC software after each measurement of the total cells and the non-viable cells. · First, the total cell number and second, non-viable cell number were measured and then the cell viability is calculated as subtracting non- viable cell counting numbers from total cell counting. -
Page 24: Error Code
3. If this message come up again after removing a chip, contact your local distributor or sales@nanoentek.com Frames with errors are over 50% of total counting frame. Frame with error is a frame that contains cells whose diameter is larger than 30μm. -
Page 25: Maintenance And Cleaning
1. ADAM-MC does not need regular maintenance. Maintenance 2. ADAM-MC has no replacement of consumable materials. and cleaning 3. Clean the exposed outer surface of ADAM-MC using a soft cloth and isopropyl alcohol or deionizers water. CAUTION • Dispose of wipes in an appropriately labeled solvent contaminated waste container. -
Page 26: Software Installation
ADAM-MC. • All measurement results are saved on the memory of ADAM-MC, automatically. • User can download the data from the memory of ADAM-MC and export it to Excel (*.xls) format. • User can delete data from memory of ADAM-MC or can save captured images into desktop or laptop hard drive. -
Page 27: Installation
Software installation To install the ADAM-MC operation software, follow the directions Installation as below: 1. Insert the installation CD-ROM into the computer. Then open the file “Setup_ADAM_v1.x.x.x.exe”. • Report program can be installed in Windows 2000, XP or higher version. - Page 28 Software installation 5. After choosing installation Installation folder, click “Install” to proceed with the installation. 6. The computer activates the “Installation of the Software”. ☞ Initial installation folder is “C:\Program Files\Digital Bio\ADAM”. 7. Report program will be installed automatically. 8. Click “Ok” to finish the installation.
-
Page 29: Function Guide
Report Program are handled by using these buttons. (see p.29 for more detailed information of each button) Data List All saved data in ADAM-MC will be loaded and shown in data list section. Information Operation and counting results of each frame will be displayed here. -
Page 30: Function Buttons
Start cell counting buttons Park (Lock) stage of ADAM-MC Eject chip holder out of ADAM-MC Load the experiment data from the memory of the main device. Delete all of the loaded data and memory of the main device. Transfer the data list to Excel format and saves it. -
Page 31: Data List
“Saved experiment”. Up to 200 counting results are automatically saved to ADAM-MC memory. When memory of ADAM-MC is full, new counting result will replace old data. These data can be exported as Excel Sheet (*.xls) and stored in personal computer or can be erased from ADAM-MC memory. -
Page 32: Graph
Graph This section shows information of cell size distribution and counting results of each frame that ADAM-MC captured. Through cell size graph, you can figure out whether there are cell clumps or aggregates. In case of counting evenly distributed cells without any aggregation, there should be a single peak on distribution of cell size. -
Page 33: Sample Image Of Error Message
Software installation Sample image of error message ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc. -
Page 34: Trouble Shooting
Trouble shooting Problem Cause Solution ADAM-MC does not · Power switch in off position. 1. Check power switch on back of unit. power up · No power from outlet. 2. Check power source. · Bad power cord. 3. Replace. Inaccurate result ·... -
Page 35: Warranty
Warranty NanoEntek warrants that the ADAM-MC will be free from defects in Warranty material and workmanship for a period of one (1) year from date of purchase. If any defects occur in the ADAM-MC during this warranty period, NanoEntek will repair or replace the defective parts at its discretion without charge. -
Page 36: Product List
Product list Additional Cat. No. Product Contents Quantity product list ADM-001 External video monitor 7” LCD Monitor (optional) AD2K-200 AccuChip2X Kit 200 pcs AccuChip2X 12.5 ml AccuStain solution T 12.5 ml AccuStain solution N AD4K-200 AccuChip4X Kit 200 pcs AccuChip4X 12.5 ml AccuStain solution T 12.5 ml AccuStain... - Page 37 ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc.
- Page 39 ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc.
- Page 40 Contact Information E-mail : sales@nanoentek.com Website : www.nanoentek.com Developed and Manufactured by NanoEntek, Inc. NanoEntek, Inc. 851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea Tel: +82-2-6220-7940, Fax: +82-2-6220-7999 NanoEntek America, Inc. 220 Bear Hill Road, Suite 102, Waltham, MA 02451, USA...