Related Manuals for BTX ECM 830
Summary of Contents for BTX ECM 830
- Page 1 User’s Manual 830 Electroporation System ® 45-0052 ® 830 Electroporator only (110 V) 45-0052INT ® 830 Electroporator only (220 V) Publication 5501-001-REV-E...
- Page 2 WEEE/RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers: We are committed to being a good corporate citizen. As part of that commitment, we strive to maintain an environmentally conscious manufacturing operation. The European Union (EU) has enacted two Directives, the first on product recycling (Waste Electrical and Electronic Equipment, WEEE) and the second limiting the use of certain substances (Restriction on the use of Hazardous Substances, RoHS).
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Page 3: Table Of Contents
® 830 Electroporation System Table of Contents General Information: Serial Number ..............2 Calibration ..............2 Warranty ..............2-3 Service ................4 Repair Facilities and Parts ..........5 General Safety Summary..........6-7 Electrical & Technical Specifications ......8-9 General Specifications............10 Introduction: Features ................11 Operation: Getting Started ..........12 Quick Start: Installation..............13 Connecting ..............13... - Page 4 ® 830 Electroporation System Table of Contents (continued) Appendix M: Plate Handlers ........51-52 830 Generator Troubleshooting ® Guidelines ..........53-54 Page 2 www.btxonline.com...
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Page 5: General Information
® 830 for a period of two years from the date of purchase. At its option, BTX – Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or materials. This warranty does not extend to any... -
Page 6: Service
This warranty gives you specific rights, and you may also have other rights, which vary from state to state. Service All service under the warranty will be made at the BTX - HARVARD APPARATUS, Holliston, Massachusetts facilities or an authorized service site. Owner will ship instrument prepaid to Holliston, Massachusetts, USA or the service site. -
Page 7: Repair Facilities And Parts
Repair service will be billed on the basis of labor and materials. A complete statement of time spent and materials used will be supplied. Shipment to BTX - HARVARD APPARATUS should be prepaid. Your bill will include return shipment freight charges. -
Page 8: General Safety Summary
DO NOT OPERATE WITH SUSPECTED FAILURES If you suspect there is damage to this product, have it inspected by qualified BTX service personnel. PROVIDE PROPER VENTILATION Refer to installation instructions for details on installing the product to ensure proper ventilation. - Page 9 (Continued) DO NOT OPERATE IN WET/DAMP CONDITIONS DO NOT OPERATE IN AN EXPLOSIVE ATMOSPHERE KEEP PRODUCT SURFACES CLEAN AND DRY Should you have any safety concerns, immediately contact BTX Technical Support ( 1-800-272-2775) Safety Terms and Symbols: Terms that appear in this manual: WARNING.
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Page 10: Electrical & Technical Specifications
® 830 Electroporation System Electrical & Technical Specifications Standard Capabilities: Display Type: 20-character by 4-line liquid crystal LED backlit Power Source Voltage 100 to 240 Vac, 50 to 60 Hz, CAT II Power Pulse: 500 W Idle: 150 W Fusing T2.5 250 V Environmental Characteristics: Intended Use... - Page 11 Less than a 10% V drop at the end of the pulse with the following PL ≤ 600 µs, load 3 40 Ω constraints: BTX instruments are designed for in vitro and in vivo animal and plant applications only and are not for human Clinic. Page 9...
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Page 12: General Specifications
® 830 Electroporation System General Specifications Certifications and Compliances Overvoltage Category: CAT III Products in this Category: Distribution-level mains, fixed installation CAT II: Local-level mains, applications and portable equipment CAT I: Signal levels in special equipment or parts of equipment, telecommunications and electronics Meets requirements of Directive 89/336/EEC for Electromagnetic Compatibility (EC) and Low-Voltage Directive 73/23/EEC for Product Safety. -
Page 13: Introduction
Introduction The ECM ® 830 is a square wave electroporation system designed for in vitro and in vivo electroporation applications. BTX square wave technology offers researchers the ability to transfect cells efficiently and with higher cell viabilites. 830 features include: ®... -
Page 14: Operation: Getting Started
830 Pulse Generator (1) Power Cord (1) Model 830E Electronic Manual (1) BTX Electroporation Systems may be customized with the addition of various electrodes and accessories. The following items com- plete a typical system order: Model 630B Electroporation Safety Stand (1) -
Page 15: Quick Start
® 830 Electroporation System Quick Start Installation 1. Install on a bench or work table. 2. Allow a 1 to 2 inch clearance for proper cooling. It is normal for the instrument to be slightly warmer than its’ operating environment. 3. -
Page 16: Instrument Controls
® 830 Electroporation System Quick Start (Continued) Instrument Controls Parameter Control Knob Start Switch Display High Voltage ™ Power Smart Link Output Switch Power Switch Switch located on the lower right front panel. Press once to initialize the ECM ® 830 and once more to turn off. - Page 17 ® 830 Electroporation System Quick Start (Continued) Parameter Control Knob The Parameter Control Knob is a rotary encoder controlling both the parameter (i.e. voltage) and the value of the parameter under control (i.e. 50 volts). The display will indicate which parameter is under control by the presence of an arrow to the left of the parameter.
- Page 18 ® 830 Electroporation System Quick Start (Continued) Voltage The Voltage indicates the set voltage in volts. The low voltage mode range is 5 V to 500 V in 1 V increments and the high voltage mode range is 505 V to 3000 V in 5 V increments. A beep is heard when transitioning between LV and HV modes.
- Page 19 ® 830 Electroporation System Quick Start (Continued) Figure 2 # Pulses The # Pulses indicates the number of set pulses from 1-99. Interval The Interval indicates the time duration between pulses. The interval range is 100 ms to 10.0 seconds, switching from units of ms to seconds after 999 ms, with 0.1 sec resolution.
- Page 20 ® 830 Electroporation System Quick Start (Continued) View To view the appropriate program parameters, push the parameter control knob to move the arrow to the left of the program number. Select the appropriate program number by rotating the parameter control knob, then hold the knob in, releasing after a new screen is displayed.