BTX ECM 630 User Manual

BTX ECM 630 User Manual

Electroporation system
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ECM
630 Electroporation System
®
MA1 45-0051
MA1 45-0051int
User's Manual
ECM
®
630 Electroporator only (110V)
ECM
®
630 Electroporator only (220V)
Publication 5501-003-REV-C

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Summary of Contents for BTX ECM 630

  • Page 1 User’s Manual 630 Electroporation System ® MA1 45-0051 ® 630 Electroporator only (110V) MA1 45-0051int ® 630 Electroporator only (220V) Publication 5501-003-REV-C...
  • Page 2 WEEE/RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers: We are committed to being a good corporate citizen. As part of that commitment, we strive to maintain an environmentally conscious manufacturing operation. The European Union (EU) has enacted two Directives, the first on product recycling (Waste Electrical and Electronic Equipment, WEEE) and the second limiting the use of certain substances (Restriction on the use of Hazardous Substances, RoHS).
  • Page 3: Table Of Contents

    ® 630 Electroporation System Table of Contents General Information: Serial Number ..............2 Calibration ..............2 Warranty ..............2-3 Service ................3-4 Repair Facilities and Parts ..........4 General Safety Summary..........5-6 Electrical & Technical Specifications ......7 General Specifications............8 Introduction: ® 630 Features ............9 Operation: Getting Started ..........10 Quick Start: Installation ..............11 Connecting ..............11...
  • Page 4: General Information

    ® 630 for a period of two years from the date of purchase. At its option, BTX – Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or materials. This warranty does not extend to any...
  • Page 5: Service

    This warranty gives you specific rights, and you may also have other rights, which vary from state to state. Service All service under the warranty will be made at the BTX - HARVARD APPARATUS, Holliston, Massachusetts facilities or an authorized service site. Owner will ship instrument prepaid to Holliston, Massachusetts, USA or the service site.
  • Page 6: Repair Facilities And Parts

    Repair service will be billed on the basis of labor and materials. A complete statement of time spent and materials used will be supplied. Shipment to BTX - HARVARD APPARATUS should be prepaid. Your bill will include return shipment freight charges.
  • Page 7: General Safety Summary

    Refer to installation instructions for details on installing the product to ensure proper ventilation. DO NOT OPERATE IN WET/DAMP CONDITIONS DO NOT OPERATE IN AN EXPLOSIVE ATMOSPHERE KEEP PRODUCT SURFACES CLEAN AND DRY Should you have any safety concerns, immediately contact BTX Technical Services (1-800-272-2775) Page 5 www.btxonline.com...
  • Page 8 ® 630 Electroporation System General Safety Summary (Continued) Safety Terms and Symbols: Terms that appear in this manual: WARNING. Warning statements identify conditions or practices that could result in injury or loss of life. CAUTION. Caution statements identify conditions or practices that could result in damage to these products or other property.
  • Page 9: Electrical & Technical Specifications

    ® 630 Electroporation System Electrical & Technical Specifications Standard Capabilities: Display Type: 20-character by 4-line liquid crystal Display. LED backlit Power Source 100 to 240 Vac, 50 to 60 Hz, Voltage CAT II Power 500 W (Pulsed), 50 W (Idle) Fusing 2.5 A, T rating 250 V Environmental Characteristics:...
  • Page 10: General Specifications

    ® 630 Electroporation System General Specifications Certifications and Compliances Overvoltage Category: CAT III: Products in this Category: Distribution-level mains, fixed installation. CAT II: Local-level mains, applications, portable equipment. CAT I: Signal levels in special equipment or parts of equipment, telecommunications, electronics. Meets requirements of Directive 89/336/EEC for Electromagnetic Compatibility (EC) and Low-Voltage Directive 73/23/EEC for Product Safety.
  • Page 11: Introduction

    This system incorporates features that make it the most technically advanced exponential decay electroporation system currrently available. 630 features include: ® • The generator utilizes the new BTX Power Platform Technology design and novel digital user interface. • The revolutionary Precision Pulse ™ System provides the researcher unparalleled power in controlling the time constant.
  • Page 12: Operation: Getting Started

    630 Pulse Generator (1) Power Cord (1) Model 630E Electronic Manual (1) BTX Electroporation Systems may be customized with the addition of various electrodes and accessories. The following items com- plete a typical system order: Model 630B Electroporation Safety Stand (1)
  • Page 13: Quick Start

    ® 630 Electroporation System Quick Start Installation 1. Install on a bench or work table. 2. Allow a 1 to 2 inch clearance for proper cooling. It is normal for the instrument to be slightly warmer than its’ operating environment. 3.
  • Page 14: Instrument Controls

    ® 630 Electroporation System Quick Start (Continued) Instrument Controls Pulse Switch Power High Voltage Parameter Display Switch Output Control Knob Power Switch 1. Electronic Power Switch located on the lower right front panel. 2. Press once to initialize the ECM ®...
  • Page 15 ® 630 Electroporation System Quick Start (Continued) Parameter Control Knob The Parameter Control Knob is a rotary encoder controlling both the parameter under control and the value of the parameter under control. 1. The display will indicate which parameter is under control by the presence of an arrow to the left of the parameter.
  • Page 16 ® 630 Electroporation System Quick Start (Continued) Resistor 1. Resistor indicates the set resistance in . 2. The resistance range is 25 to 1575 in 25 increments. 3. In the LV mode there is an additional resistor selection of “None”. This indicates that there is no timing resistor in the pulsing circuit, thus mimicking settings for competitive electroporators in which there is either no resistor to select, or the resistance is termed “unlimited.
  • Page 17 ® 630 Electroporation System Quick Start (Continued) View 1. To view the appropriate program parameters, push the parameter control knob to move the arrow to the left of the program number. 2. Select the appropriate program number by rotating the parameter control knob, then hold the knob in, releasing after a new screen is displayed.
  • Page 18 ® 630 Electroporation System Quick Start (Continued) Capacitor The value of the timing capacitance. Time constant t=RC Resistor The value of the timing resistance. Time constant t=RC Save Save current setup parameters to nonvolatile memory View Show the setup parameters stored in nonvolatile memory Load Load setup parameters from memory to use Status Messages...
  • Page 19 ® 630 Electroporation System Quick Start (Continued) Pulse Delivered Screen 1. The Pulse Delivered Screen is displayed following the delivery of a pulse and is indicated by the display “Pulse Delivered” on the top line of the screen. 2. Press the knob to return to the Set Parameters Screen. 3.
  • Page 20: Operating Basics

    Use with Alternative Electrode Chambers and Applications The ECM ® 630 may be used to power all BTX electroporation cuvettes and electrodes. Follow the above instructions in conjunction with instructions provided for the specific electrode. Refer to Appendix A for graphical representation of operating ranges of various chambers and applicators with the ECM ®...

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